Regulatory impact of the C-terminal tail on charge transfer pathways in drosophila cryptochrome

Interconnected transcriptional and translational feedback loops are at the core of the molecular mechanism of the circadian clock. Such feedback loops are synchronized to external light entrainment by the blue light photoreceptor cryptochrome (CRY) that undergoes conformational changes upon light absorption by an unknown photoexcitation mechanism. Light-induced charge transfer (CT) reactions in Drosophila CRY (dCRY) are investigated by state-of-the-art simulations that reveal a complex, multi-redox site nature of CT dynamics on the microscopic level. The simulations consider redox-active chromophores of the tryptophan triad (Trp triad) and further account for pathways mediated by W314 and W422 residues proximate to the C-terminal tail (CTT), thus avoiding a pre-bias to specific W-mediated CT pathways. The conducted dissipative quantum dynamics simulations employ microscopically derived model Hamiltonians and display complex and ultrafast CT dynamics on the picosecond timescale, subtly balanced by the electrostatic environment of dCRY. In silicio point mutations provide a microscopic basis for rationalizing particular CT directionality and demonstrate the degree of electrostatic control realized by a discrete set of charged amino acid residues. The predicted participation of CT states in proximity to the CTT relates the directionality of CT reactions to the spatial vicinity of a linear interaction motif. The results stress the importance of CTT directional charge transfer in addition to charge transfer via the Trp triad and call for the use of full-length CRY models including the interactions of photolyase homology region (PHR) and CTT domains. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

COS-Weak: Probing the CGM using analogs of weak Mg II absorbers at z < 0.3

We present a sample of 34 weak metal line absorbers at $z< 0.3$ complied via the simultaneous detections ($3\sigma$) of the SiII$\lambda1260$ and CII$\lambda1334$ absorption lines, with $W_{r}$(SiII)$<0.2$ \AA\ and $W_{r}$(CII)$<0.3$ \AA, in archival HST/COS spectra. Our sample increases the number of known low-$z$ "weak absorbers" by a factor of $>5$. The column densities of HI and low-ionization metal lines obtained from Voigt profile fitting are used to build simple photoionization models using CLOUDY. The inferred densities and total hydrogen column densities are in the ranges of $-3.3 < \log n_{\rm H}/{\rm cm^{-3}} < -2.4$ and $16.0 < \log N_{\rm H}/{\rm cm^{-2}}<20.3$, respectively. The line of sight thicknesses of the absorbers have a wide range of $\sim$1 pc$-$50 kpc with a median value of $\sim$500 pc. The high-ionization OVI absorption, detected in 12/18 cases, always stems from a different gas-phase. Most importantly, 85% (50%) of these absorbers show a metallicity of $\rm [Si/H] > -1.0$ (0.0). The fraction of systems showing high metallicity (i.e., $\rm [Si/H]>-1.0$) in our sample is significantly higher than the HI-selected sample (Wotta et al. 2016) and the galaxy-selected sample (Prochaska et al. 2017) of absorbers probing the circum-galactic medium (CGM) at similar redshift. A search for galaxies has revealed a significant galaxy-overdensity around these weak absorbers compared to random places with a median impact parameter of 166 kpc to the nearest galaxy. Moreover, we find the presence of multiple galaxies in $\sim80$% of the cases, suggesting group environments. The observed $d\mathcal{N}/dz$ of $0.8\pm0.2$ indicates that such metal-enriched, compact, dense structures are ubiquitous in the halos of low-$z$ galaxies that are in groups. We suggest that these are transient structures that are related to outflows and/or stripping of metal-rich gas from galaxies.Comment: Published (2018MNRAS.476.4965M) after minor revision. Appendix A is newly added

Россия в условиях современной экономической глобализации

В статье автор пытается определить содержание глобализации как экономической категории. Рассматривает различные подходы к определению глобализации. Выделяет наиболее характерные признаки глобализации, положительные и отрицательные стороны этого явления. На базе анализа глобализации как экономического явления он пытается сделать выводы для российской экономической политики. Акцентирует внимание на необходимости адекватно реагировать на вызовы мировой капиталистической реальности, обращает внимание на то, что абсолютизация рыночных начал дискредитирует идею глобализации. Делает вывод о необходимости усиления внимания к социальным аспектам и противоречиям глобализации с целью минимизации негативных последствий этого явления

Selektive Biofunktionalisierung dreidimensionaler Mikrostrukturen

Zellen werden in ihrer natürlichen Umgebung von einer Vielzahl von Faktoren beeinflusst. Für zukünftige Zellexperimente wäre es optimal alle Faktoren (Geometrie, Nanotopographie, Steifigkeit, räumliche und quantitative Ligandenverteilung) kontrollieren zu können um Aufschluss über eine optimale Wachstumsumgebung für Zellen zu erhalten. Deshalb werden in dieser Arbeit verschiedene Methoden zur selektiven Biofunktionalisierung dreidimensionaler Mikrostrukturen vorgestellt

Classical and Quantum Signatures of Quantum Phase Transitions in a (Pseudo) Relativistic Many-Body System

We identify a (pseudo) relativistic spin-dependent analogue of the celebrated quantum phase transition driven by the formation of a bright soliton in attractive one-dimensional bosonic gases. In this new scenario, due to the simultaneous existence of the linear dispersion and the bosonic nature of the system, special care must be taken with the choice of energy region where the transition takes place. Still, due to a crucial adiabatic separation of scales, and identified through extensive numerical diagonalization, a suitable effective model describing the transition is found. The corresponding mean-field analysis based on this effective model provides accurate predictions for the location of the quantum phase transition when compared against extensive numerical simulations. Furthermore, we numerically investigate the dynamical exponents characterizing the approach from its finite-size precursors to the sharp quantum phase transition in the thermodynamic limit

Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity

Due to the size and opacity of vertebrate brains, it has until now been impossible to simultaneously record neuronal activity at cellular resolution across the entire adult brain. As a result, scientists are forced to choose between cellular-resolution microscopy over limited fields-of-view or whole-brain imaging at coarse-grained resolution. Bridging the gap between these spatial scales of understanding remains a major challenge in neuroscience. Here, we introduce blazed oblique plane microscopy to perform brain-wide recording of neuronal activity at cellular resolution in an adult vertebrate. Contrary to common belief, we find that inferences of neuronal population activity are near-independent of spatial scale: a set of randomly sampled neurons has a comparable predictive power as the same number of coarse-grained macrovoxels. Our work thus links cellular resolution with brain-wide scope, challenges the prevailing view that macroscale methods are generally inferior to microscale techniques and underscores the value of multiscale approaches to studying brain-wide activity