Implantation of 3D-Printed Patient-Specific Aneurysm Models into Cadaveric Specimens: A New Training Paradigm to Allow for Improvements in Cerebrovascular Surgery and Research.

AimTo evaluate the feasibility of implanting 3D-printed brain aneurysm model in human cadavers and to assess their utility in neurosurgical research, complex case management/planning, and operative training.MethodsTwo 3D-printed aneurysm models, basilar apex and middle cerebral artery, were generated and implanted in four cadaveric specimens. The aneurysms were implanted at the same anatomical region as the modeled patient. Pterional and orbitozygomatic approaches were done on each specimen. The aneurysm implant, manipulation capabilities, and surgical clipping were evaluated.ResultsThe 3D aneurysm models were successfully implanted to the cadaveric specimens' arterial circulation in all cases. The features of the neck in terms of flexibility and its relationship with other arterial branches allowed for the practice of surgical maneuvering characteristic to aneurysm clipping. Furthermore, the relationship of the aneurysm dome with the surrounding structures allowed for better understanding of the aneurysmal local mass effect. Noticeably, all of these observations were done in a realistic environment provided by our customized embalming model for neurosurgical simulation.Conclusion3D aneurysms models implanted in cadaveric specimens may represent an untapped training method for replicating clip technique; for practicing certain approaches to aneurysms specific to a particular patient; and for improving neurosurgical research

Optimal power flow for resistive DC networks: a port-hamiltonian approach

This paper studies the optimal power flow problem for resistive DC networks. The gradient method algorithm is written in a port-Hamiltonian form and the stability of the resulting dynamics is studied. Stability conditions are provided for general cyclic networks and a solution, when these conditions fail, is proposed. In addition, the results are exemplified by means of numerical simulations.Postprint (published version

Analysis of SPME or SBSE extracted volatile compounds from cooked cured pork ham differing in intramuscular fat profiles

We studied the influence of the IMF content (high - HI\ua0vs. low - LI) and the fatty acid saturation profile on cooked cured pork ham volatiles. LI hams had higher PUFA and lower MUFA contents than HI hams. Using SPME we identified 29 compounds novel to cooked cured pork ham profiles, mostly lipid derivatives. Group differences were related to the PUFA/MUFA contents but not to the IMF content. Differences were also identified in amino acid breakdown derivatives with potential aroma implications. The SBSE method, a novelty in pork meat science, revealed differences which included board taint-related volatiles, terpenes and 36 novel compounds; 14 of these compounds were only found by the SBSE method

Self-induced large-scale motions in a three-dimensional diffuser

A direct numerical simulation of a three-dimensional diffuser at Reynolds number Re = 10,000 (based on inlet bulk velocity) has been performed using a low-dissipation finite element code. The geometry chosen for this work is the Stanford diffuser, introduced by Cherry et al. (Int. J. Heat Fluid Flow 29:803–811, 2008). Results have been exhaustively compared with the published data with a quite good agreement. Additionally, further turbulent statistics have been provided such as the Reynolds stresses or the turbulent kinetic energy. A proper orthogonal decomposition and a dynamic mode decomposition analyses of the main flow variables have been performed to identify the main characteristics of the large-scale motions. A combined, self-induced movement of the large-scales has been found to originate in the top-right expansion corner with two clear features. A low-frequency diagonal cross-stream travelling wave first reported by Malm et al. (J. Fluid Mech. 699:320–351, 2012), has been clearly identified in the spatial modes of the stream-wise velocity components and the pressure, associated with the narrow band frequency of St¿[0.083,0.01] . This movement is caused by the geometrical expansion of the diffuser in the cross-stream direction. A second low-frequency trait has been identified associated with the persisting secondary flows and acting as a back and forth global accelerating-decelerating motion located on the straight area of the diffuser, with associated frequencies of St<0.005 . The smallest frequency observed in this work has been St=0.0013 . This low-frequency observed in the Stanford diffuser points out the need for longer simulations in order to obtain further turbulent statistics.The research leading to this work has been partially funded by the European Project NextSim which has received funding from the European High-Performance Computing Joint Undertaking (JU) under grant agreement No 956104 and co-founded by the Spanish Agencia Estatal de Investigacion (AEI) under grant agreement PCI2021-121962. Benet Eiximeno also acknowledges the financial support by the Ministerio de Economía y Competitividad, Secretaría de Estado de Investigación, Desarrollo e Innovación, Spain (Refs: PID2020-116937RB-C21 and PID2020-116937RB-C22). Oriol Lehmkuhl has been partially supported by a Ramon y Cajal postdoctoral contract (Ref: RYC2018-025949-I). He also acknowledges the support of the European Project HiFi-TURB which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 814837. We also acknowledge the Barcelona Supercomputing Center for awarding us access to the MareNostrum IV machine based in Barcelona, Spain. The authors acknowledge the support of Departament de Recerca i Universitats de la Generalitat de Catalunya to the Research Group Large-scale Computational Fluid Dynamics (Code: 2021 SGR 00902).Peer ReviewedPostprint (published version

Implantation of 3D-Printed Patient-Specific Aneurysm Models into Cadaveric Specimens: A New Training Paradigm to Allow for Improvements in Cerebrovascular Surgery and Research

Aim. To evaluate the feasibility of implanting 3D-printed brain aneurysm model in human cadavers and to assess their utility in neurosurgical research, complex case management/planning, and operative training. Methods. Two 3D-printed aneurysm models, basilar apex and middle cerebral artery, were generated and implanted in four cadaveric specimens. The aneurysms were implanted at the same anatomical region as the modeled patient. Pterional and orbitozygomatic approaches were done on each specimen. The aneurysm implant, manipulation capabilities, and surgical clipping were evaluated. Results. The 3D aneurysm models were successfully implanted to the cadaveric specimens’ arterial circulation in all cases. The features of the neck in terms of flexibility and its relationship with other arterial branches allowed for the practice of surgical maneuvering characteristic to aneurysm clipping. Furthermore, the relationship of the aneurysm dome with the surrounding structures allowed for better understanding of the aneurysmal local mass effect. Noticeably, all of these observations were done in a realistic environment provided by our customized embalming model for neurosurgical simulation. Conclusion. 3D aneurysms models implanted in cadaveric specimens may represent an untapped training method for replicating clip technique; for practicing certain approaches to aneurysms specific to a particular patient; and for improving neurosurgical research

On the wake dynamics of an oscillating cylinder via proper orthogonal decomposition

The coherent structures and wake dynamics of a two-degree-of-freedom vibrating cylinder with a low mass ratio at Re=5300 are investigated by means of proper orthogonal decomposition (POD) of a numerical database generated using large-eddy simulations. Two different reduced velocities of U*=3.0 and U*=5.5, which correspond with the initial and super-upper branches, are considered. This is the first time that this kind of analysis is performed in this kind of system in order to understand the role of large coherent motions on the amplification of the forces. In both branches of response, almost 1000 non-correlated in-time velocity fields have been decomposed using the snapshot method. It is seen that a large number of modes is required to represent 95% of the turbulent kinetic energy of the flow, but the first two modes contain a large percentage of the energy as they represent the wake large-scale vortex tubes. The energy dispersion of the high-order modes is attributed to the cylinder movement in the inline and cross-stream directions. Substantially different POD modes have been found in the two branches. While the first six modes resemble those observed in the static cylinder or in the initial branch of a one-degree of freedom cylinder in the initial branch, the modes not only contain information about the wake vortexes in the super-upper branch but also about the formation of the 2T vortex pattern and the Taylor–Görtler structures. It is shown that the 2T vortex pattern is formed by the interplay between the Taylor–Görtler stream-wise vortical structures and the cylinder movement and is responsible for the increase in the lift force and larger elongation in the super-upper branch.This work has been partially financially supported by the Ministerio de Economía, Industria y Competitividad, Secretaría de Estado de Investigación, Desarrollo e Innovación, Spain (Ref. PID2020-116937RB-C21, PID2020-116937RB-C22), and by the European High-Performance Computing Joint Undertaking (JU) under grant agreement No 956104. The JU receives support from the European Union’s Horizon 2020 research and innovation programme and Spain, France, Germany. O. Lehmkuhl work is financed by a Ramón y Cajal postdoctoral contract by the Ministerio de Economía y Competitividad, Secretaría de Estado de Investigación, Desarrollo e Innovación, Spain (RYC2018-025949-I).Peer ReviewedPostprint (published version

Inference of the life cycle of environmental phages from genomic signature distances to their hosts

The environmental impact of uncultured phages is shaped by their preferred life cycle (lytic or lysogenic). However, our ability to predict it is very limited. We aimed to discriminate between lytic and lysogenic phages by comparing the similarity of their genomic signatures to those of their hosts, reflecting their co-evolution. We tested two approaches: (1) similarities of tetramer relative frequencies, (2) alignment-free comparisons based on exact k = 14 oligonucleotide matches. First, we explored 5126 reference bacterial host strains and 284 associated phages and found an approximate threshold for distinguishing lysogenic and lytic phages using both oligonucleotide-based methods. The analysis of 6482 plasmids revealed the potential for horizontal gene transfer between different host genera and, in some cases, distant bacterial taxa. Subsequently, we experimentally analyzed combinations of 138 Klebsiella pneumoniae strains and their 41 phages and found that the phages with the largest number of interactions with these strains in the laboratory had the shortest genomic distances to K. pneumoniae. We then applied our methods to 24 single-cells from a hot spring biofilm containing 41 uncultured phage-host pairs, and the results were compatible with the lysogenic life cycle of phages detected in this environment. In conclusion, oligonucleotide-based genome analysis methods can be used for predictions of (1) life cycles of environmental phages, (2) phages with the broadest host range in culture collections, and (3) potential horizontal gene transfer by plasmids

Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1

HIV-1; Siglec-1; CervixVIH-1; Siglec-1; CèrvixVIH-1; Siglec-1; CérvixAntigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. Yet, the molecular mechanism at the cervical mucosa underlying this viral transmission pathway remains unknown. Here we identified a subset of HLA-DR+ CD14+ CD11c+ cervical DCs at the lamina propria of the ectocervix and the endocervix that expressed the type-I interferon inducible lectin Siglec-1 (CD169), which promoted viral uptake. In the cervical biopsy of a viremic HIV-1+ patient, Siglec-1+ cells harbored HIV-1-containing compartments, demonstrating that in vivo, these cells trap viruses. Ex vivo, a type-I interferon antiviral environment enhanced viral capture and trans-infection via Siglec-1. Nonetheless, HIV-1 transfer via cervical DCs was effectively prevented with antibodies against Siglec-1. Our findings contribute to decipher how cervical DCs may boost HIV-1 replication and promote systemic viral spread from the cervical mucosa, and highlight the importance of including inhibitors against Siglec-1 in microbicidal strategies

Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1

Altres ajuts: JM-P and NI-U are supported by the Spanish Secretariat of State of Research, Development and Innovation through grant SAF2016-80033-R. MG is supported by a Marie Curie Career Integration Grant (CIG) from the European Commission and by the Pla estratègic de recerca i innovació en salut (PERIS), from the Catalan government.Antigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. Yet, the molecular mechanism at the cervical mucosa underlying this viral transmission pathway remains unknown. Here we identified a subset of HLA-DR+ CD14+ CD11c+ cervical DCs at the lamina propria of the ectocervix and the endocervix that expressed the type-I interferon inducible lectin Siglec-1 (CD169), which promoted viral uptake. In the cervical biopsy of a viremic HIV-1+ patient, Siglec-1+ cells harbored HIV-1-containing compartments, demonstrating that in vivo, these cells trap viruses. Ex vivo, a type-I interferon antiviral environment enhanced viral capture and trans-infection via Siglec-1. Nonetheless, HIV-1 transfer via cervical DCs was effectively prevented with antibodies against Siglec-1. Our findings contribute to decipher how cervical DCs may boost HIV-1 replication and promote systemic viral spread from the cervical mucosa, and highlight the importance of including inhibitors against Siglec-1 in microbicidal strategies

Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1

Antigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. Yet, the molecular mechanism at the cervical mucosa underlying this viral transmission pathway remains unknown. Here we identified a subset of HLA-DR+ CD14+ CD11c+ cervical DCs at the lamina propria of the ectocervix and the endocervix that expressed the type-I interferon inducible lectin Siglec-1 (CD169), which promoted viral uptake. In the cervical biopsy of a viremic HIV-1+ patient, Siglec-1+ cells harbored HIV-1-containing compartments, demonstrating that in vivo, these cells trap viruses. Ex vivo, a type-I interferon antiviral environment enhanced viral capture and trans-infection via Siglec-1. Nonetheless, HIV-1 transfer via cervical DCs was effectively prevented with antibodies against Siglec-1. Our findings contribute to decipher how cervical DCs may boost HIV-1 replication and promote systemic viral spread from the cervical mucosa, and highlight the importance of including inhibitors against Siglec-1 in microbicidal strategies