High pCO 2 levels affect metabolic rate, but not feeding behavior and fitness, of farmed giant mussel Choromytilus chorus

Indexación: Scopus.Acknowledgements. We thank Luisa Saavedra and Araceli Rodriguez-Romero for their help in the field and during laboratory activities. We also acknowledge Laura Ramajo for help with AT estimations. Emily Giles Neill provided valuable comments that greatly improved the manuscript. Special thanks are due to the reviewers and the editor for very constructive comments on the manuscript. This study was supported by the Millennium Nucleus Center for the Study of Multiple drivers on Marine Socio-Ecological Systems (MUSELS) funded by MINECON NC120086, PIA CONICYT ACT-172037 and FONDECYT grant nos. 1140938 and 1140092 to N.A.L. and M.A.L.Mar Ecol Prog Ser 454: 65−74 Findlay HS, Wood HL, Kendall MA, Spicer JI, Twitchett RJ, Widdicombe S (2009) Calcification, a physiological pro-cess to be considered in the context of the whole organ-ism. 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Reg Environ Change 14(Suppl 1): 19−30 Sabine C, Feely RA, Gruber N, Key RM and others (2004) The oceanic sink of anthropogenic CO2. Science 305: 367–371 SERNAPESCA (Servicio Nacional de Pesca y Acuicultura) (2014) Anuarios estadísticos del Servicio Nacional de Pesca y Acuicultura. www.sernapesca.cl Solórzano L (1969) Determination of ammonia in natural waters by the phenolhypochlorite method. Limnol Oce - anogr 14: 799−801 Thomsen J, Melzner F (2010) Moderate seawater acidifica-tion does not elicit long-term metabolic depression in the blue mussel Mytilus edulis. Mar Biol 157: 2667−2676 Thomsen J, Casties I, Pansch C, Körtzinger A, Melzner F (2013) Food availability outweighs ocean acidification effects in juvenile Mytilus edulis: laboratory and field experiments. Glob Change Biol 19: 1017−1027 Thomsen J, Stapp LS, Haynert K, Schade H, Danelli M, Lannig G, Melzner F (2017) Naturally acidified habitat selects for ocean acidification-tolerant mussels. Sci Adv 3: e1602411 Toro B, Navarro JM, Palma-Fleming H (2003) Relationship between bioenergetics responses and organic pollutants in the giant mussel, Choromytilus chorus (Mollusca: Mytilidae). Aquat Toxicol 63: 257−269 Torres R, Pantoja S, Harada N, González HE, Daneri G, Frangopulos M, Fukasawa M (2011) Air-sea CO2 fluxes along the coast of Chile: from CO2 outgassing in central northern upwelling waters to CO2 uptake in southern Patagonian fjords. J Geophys Res 116: C09006 Torres R, Manriquez PH, Duarte C, Navarro JM, Lagos NA, Vargas CA, Lardies MA (2013) Evaluation of a semi - automatic system for long-term seawater carbonate chemistry manipulation. Rev Chil Hist Nat 86: 443−451 Vargas CA, Aguilera V, Martín V, Manríquez P and others (2015) CO2-driven ocean acidification disrupts the filter feeding behavior in Chilean gastropod and bivalve spe-cies from different geographic localities. Estuaries Coasts 38: 1163−1177 Vargas CA, Lagos NA, Lardies MA, Duarte C and others (2017) Species-specific responses to ocean acidification should account for local adaptation and adaptive plasti-city. Nature Ecol Evol 1: 0084 Vargas CA, Cuevas LA, Silva N, Gonzalez HE, Pol-Holz D, Narvaez DA (2018) Influence of glacier melting and river discharges on the nutrient distribution and DIC recycling in the southern Chilean Patagonia. J Geophys Res Bio-geosci 123: 256−270 Velasco LA, Navarro JM (2003) Energetic balance of infau-nal (Mulinia edulis King, 1831) and epifaunal (Mytilus chilensis Hupé, 1854) bivalves in response to wide varia-tions in concentration and quality of seston. J Exp Mar Biol Ecol 296: 79−92 Vihtakari M, Hendriks IE, Holding J, Renaud PE, Duarte CM, Havenhand JN (2013) Effects of ocean acidification and warming on sperm activity and early life stages of the Mediterranean mussel (Mytilus galloprovincialis). Water 5: 1890−1915 Wang Y, Li L, Hu M, Lu W (2015) Physiological energetic of the thick shell mussel Mytilus coruscus exposed to sea-water acidification and thermal stress. Sci Total Environ 514: 261−272 White MM, McCorkle DC, Mullineaux LS, Cohen AL (2013)Benthic habitats such as intertidal areas, sandy or rocky shores, upwelling zones, and estuaries are characterized by variable environmental conditions. This high variability of environmental stressors such as temperature, salinity, and pH/pCO 2 levels have been shown to impose restrictions on organismal performance. The giant mussel Choromytilus chorus forms intertidal and subtidal mussel beds in estuarine zones associated with fjords occurring in southern Chile and is an important aquacultural resource in Patagonia. In this study, we estimated the sensitivity of physiological traits and energy balance of C. chorus juveniles exposed to 3 pCO 2 treatments (500, 750, and 1200 μatm) for 30 d. Results showed that in acidified, high pCO 2 conditions, C. chorus juveniles had increased metabolic rates; however, other physiological traits (clearance and ingestion rates, ammonia excretion, absorption efficiency, growth rate, biomass production, net calcification, and dissolution rates) were not affected. These results suggest that when subjected to acidification, the adaptive response of C. chorus triggers tradeoffs among physiological traits that favor sustained feeding and growth in order to combat increased metabolic stress. As has been reported for other marine organisms, chronic exposure to variable pH/pCO 2 in their native habitats, such as estuarine zones, could explain the differential acclimatization capacity of giant mussels to cope with the increase in pCO 2 . Additionally, the fact that the mussels did not suffer from mortality indicates that increased pCO 2 levels may have chronic, but not lethal, effects on this species under these experimental conditions. © The authors 2017.https://www.int-res.com/abstracts/aei/v10/p267-278

Free-form optics for Fresnel-lens-based photovoltaic concentrators

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