Dual enzyme-triggered controlled release on capped nanometric silica mesoporous supports

We thank the Spanish Government (project MAT2009-14564-C04 and CTQ2007-64735-AR07) the Generalitat Valencia (project PROMETEO/2009/016) for support. A.A. and L.M. thank the Generalitat Valenciana for their Santiago Grisolia Fellowship and VALI+D postdoctoral contract, respectively. We thank the confocal microscopy service from CIPF for technical support.Agostini, A.; Mondragón Martínez, L.; Coll Merino, MC.; Aznar Gimeno, E.; Marcos Martínez, MD.; Martínez Mañez, R.; Sancenón Galarza, F.... (2012). Dual enzyme-triggered controlled release on capped nanometric silica mesoporous supports. ChemistryOpen. 1:17-20. https://doi.org/10.1002/open.201200003S17201Saha, S., Leung, K. C.-F., Nguyen, T. D., Stoddart, J. F., & Zink, J. I. (2007). Nanovalves. Advanced Functional Materials, 17(5), 685-693. doi:10.1002/adfm.200600989Trewyn, B. G., Slowing, I. I., Giri, S., Chen, H.-T., & Lin, V. S.-Y. (2007). Synthesis and Functionalization of a Mesoporous Silica Nanoparticle Based on the Sol–Gel Process and Applications in Controlled Release. Accounts of Chemical Research, 40(9), 846-853. doi:10.1021/ar600032uAznar, E., Martínez-Máñez, R., & Sancenón, F. (2009). Controlled release using mesoporous materials containing gate-like scaffoldings. Expert Opinion on Drug Delivery, 6(6), 643-655. doi:10.1517/17425240902895980Beck, J. S., Vartuli, J. C., Roth, W. J., Leonowicz, M. E., Kresge, C. T., Schmitt, K. D., … Schlenker, J. L. (1992). A new family of mesoporous molecular sieves prepared with liquid crystal templates. Journal of the American Chemical Society, 114(27), 10834-10843. doi:10.1021/ja00053a020Wight, A. P., & Davis, M. E. (2002). Design and Preparation of Organic−Inorganic Hybrid Catalysts. Chemical Reviews, 102(10), 3589-3614. doi:10.1021/cr010334mKickelbick, G. (2004). Mesoporöse anorganisch-organische Hybridmaterialien. Angewandte Chemie, 116(24), 3164-3166. doi:10.1002/ange.200301751Kickelbick, G. (2004). Hybrid Inorganic–Organic Mesoporous Materials. Angewandte Chemie International Edition, 43(24), 3102-3104. doi:10.1002/anie.200301751Mal, N. K., Fujiwara, M., & Tanaka, Y. (2003). Photocontrolled reversible release of guest molecules from coumarin-modified mesoporous silica. Nature, 421(6921), 350-353. doi:10.1038/nature01362Mal, N. K., Fujiwara, M., Tanaka, Y., Taguchi, T., & Matsukata, M. (2003). Photo-Switched Storage and Release of Guest Molecules in the Pore Void of Coumarin-Modified MCM-41. Chemistry of Materials, 15(17), 3385-3394. doi:10.1021/cm0343296Zhu, Y., & Fujiwara, M. (2007). Installing Dynamic Molecular Photomechanics in Mesopores: A Multifunctional Controlled-Release Nanosystem. Angewandte Chemie, 119(13), 2291-2294. doi:10.1002/ange.200604850Zhu, Y., & Fujiwara, M. (2007). Installing Dynamic Molecular Photomechanics in Mesopores: A Multifunctional Controlled-Release Nanosystem. Angewandte Chemie International Edition, 46(13), 2241-2244. doi:10.1002/anie.200604850Liu, N., Chen, Z., Dunphy, D. R., Jiang, Y.-B., Assink, R. A., & Brinker, C. J. (2003). Angewandte Chemie, 115(15), 1773-1776. doi:10.1002/ange.200250189Liu, N., Chen, Z., Dunphy, D. R., Jiang, Y.-B., Assink, R. A., & Brinker, C. J. (2003). Photoresponsive Nanocomposite Formed by Self-Assembly of an Azobenzene-Modified Silane. Angewandte Chemie International Edition, 42(15), 1731-1734. doi:10.1002/anie.200250189Aznar, E., Casasús, R., García-Acosta, B., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., … Amorós, P. (2007). Photochemical and Chemical Two-Channel Control of Functional Nanogated Hybrid Architectures. Advanced Materials, 19(17), 2228-2231. doi:10.1002/adma.200601958Park, C., Lee, K., & Kim, C. (2009). Photoresponsive Cyclodextrin-Covered Nanocontainers and Their Sol-Gel Transition Induced by Molecular Recognition. Angewandte Chemie International Edition, 48(7), 1275-1278. doi:10.1002/anie.200803880Ferris, D. P., Zhao, Y.-L., Khashab, N. M., Khatib, H. A., Stoddart, J. F., & Zink, J. I. (2009). Light-Operated Mechanized Nanoparticles. Journal of the American Chemical Society, 131(5), 1686-1688. doi:10.1021/ja807798gLin, Q., Huang, Q., Li, C., Bao, C., Liu, Z., Li, F., & Zhu, L. (2010). Anticancer Drug Release from a Mesoporous Silica Based Nanophotocage Regulated by Either a One- or Two-Photon Process. Journal of the American Chemical Society, 132(31), 10645-10647. doi:10.1021/ja103415tKnežević, N. Ž., Trewyn, B. G., & Lin, V. S.-Y. (2011). Light- and pH-Responsive Release of Doxorubicin from a Mesoporous Silica-Based Nanocarrier. Chemistry - A European Journal, 17(12), 3338-3342. doi:10.1002/chem.201002960Knežević, N. Ž., Trewyn, B. G., & Lin, V. S.-Y. (2011). Functionalized mesoporous silica nanoparticle-based visible light responsive controlled release delivery system. Chemical Communications, 47(10), 2817. doi:10.1039/c0cc04424eTrewyn, B. G., Giri, S., Slowing, I. I., & Lin, V. S.-Y. (2007). Mesoporous silica nanoparticle based controlled release, drug delivery, and biosensor systems. Chemical Communications, (31), 3236. doi:10.1039/b701744hTorney, F., Trewyn, B. G., Lin, V. S.-Y., & Wang, K. (2007). Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nature Nanotechnology, 2(5), 295-300. doi:10.1038/nnano.2007.108Radu, D. R., Lai, C.-Y., Jeftinija, K., Rowe, E. W., Jeftinija, S., & Lin, V. S.-Y. (2004). A Polyamidoamine Dendrimer-Capped Mesoporous Silica Nanosphere-Based Gene Transfection Reagent. Journal of the American Chemical Society, 126(41), 13216-13217. doi:10.1021/ja046275mGiri, S., Trewyn, B. G., Stellmaker, M. P., & Lin, V. S.-Y. (2005). Stimuli-Responsive Controlled-Release Delivery System Based on Mesoporous Silica Nanorods Capped with Magnetic Nanoparticles. Angewandte Chemie, 117(32), 5166-5172. doi:10.1002/ange.200501819Giri, S., Trewyn, B. G., Stellmaker, M. P., & Lin, V. S.-Y. (2005). Stimuli-Responsive Controlled-Release Delivery System Based on Mesoporous Silica Nanorods Capped with Magnetic Nanoparticles. Angewandte Chemie International Edition, 44(32), 5038-5044. doi:10.1002/anie.200501819Fujiwara, M., Terashima, S., Endo, Y., Shiokawa, K., & Ohue, H. (2006). Switching catalytic reaction conducted in pore void of mesoporous material by redox gate control. Chemical Communications, (44), 4635. doi:10.1039/b610444dLiu, R., Zhao, X., Wu, T., & Feng, P. (2008). Tunable Redox-Responsive Hybrid Nanogated Ensembles. Journal of the American Chemical Society, 130(44), 14418-14419. doi:10.1021/ja8060886Nguyen, T. D., Liu, Y., Saha, S., Leung, K. C.-F., Stoddart, J. F., & Zink, J. I. (2007). Design and Optimization of Molecular Nanovalves Based on Redox-Switchable Bistable Rotaxanes. Journal of the American Chemical Society, 129(3), 626-634. doi:10.1021/ja065485rCasasús, R., Marcos, M. D., Martínez-Máñez, R., Ros-Lis, J. V., Soto, J., Villaescusa, L. A., … Latorre, J. (2004). Toward the Development of Ionically Controlled Nanoscopic Molecular Gates. Journal of the American Chemical Society, 126(28), 8612-8613. doi:10.1021/ja048095iCasasús, R., Climent, E., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., Soto, J., … Ruiz, E. (2008). Dual Aperture Control on pH- and Anion-Driven Supramolecular Nanoscopic Hybrid Gate-like Ensembles. Journal of the American Chemical Society, 130(6), 1903-1917. doi:10.1021/ja0756772Aznar, E., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., Soto, J., Amorós, P., & Guillem, C. (2009). pH- and Photo-Switched Release of Guest Molecules from Mesoporous Silica Supports. Journal of the American Chemical Society, 131(19), 6833-6843. doi:10.1021/ja810011pAngelos, S., Yang, Y.-W., Khashab, N. M., Stoddart, J. F., & Zink, J. I. (2009). Dual-Controlled Nanoparticles Exhibiting AND Logic. Journal of the American Chemical Society, 131(32), 11344-11346. doi:10.1021/ja9042752Angelos, S., Yang, Y.-W., Patel, K., Stoddart, J. F., & Zink, J. I. (2008). pH-Responsive Supramolecular Nanovalves Based on Cucurbit[6]uril Pseudorotaxanes. Angewandte Chemie, 120(12), 2254-2258. doi:10.1002/ange.200705211Angelos, S., Yang, Y.-W., Patel, K., Stoddart, J. F., & Zink, J. I. (2008). pH-Responsive Supramolecular Nanovalves Based on Cucurbit[6]uril Pseudorotaxanes. Angewandte Chemie International Edition, 47(12), 2222-2226. doi:10.1002/anie.200705211Angelos, S., Khashab, N. M., Yang, Y.-W., Trabolsi, A., Khatib, H. A., Stoddart, J. F., & Zink, J. I. (2009). pH Clock-Operated Mechanized Nanoparticles. Journal of the American Chemical Society, 131(36), 12912-12914. doi:10.1021/ja9010157Du, L., Liao, S., Khatib, H. A., Stoddart, J. F., & Zink, J. I. (2009). Controlled-Access Hollow Mechanized Silica Nanocontainers. Journal of the American Chemical Society, 131(42), 15136-15142. doi:10.1021/ja904982jYang, Q., Wang, S., Fan, P., Wang, L., Di, Y., Lin, K., & Xiao, F.-S. (2005). pH-Responsive Carrier System Based on Carboxylic Acid Modified Mesoporous Silica and Polyelectrolyte for Drug Delivery. Chemistry of Materials, 17(24), 5999-6003. doi:10.1021/cm051198vPark, C., Oh, K., Lee, S. C., & Kim, C. (2007). Controlled Release of Guest Molecules from Mesoporous Silica Particles Based on a pH-Responsive Polypseudorotaxane Motif. Angewandte Chemie, 119(9), 1477-1479. doi:10.1002/ange.200603404Park, C., Oh, K., Lee, S. C., & Kim, C. (2007). Controlled Release of Guest Molecules from Mesoporous Silica Particles Based on a pH-Responsive Polypseudorotaxane Motif. Angewandte Chemie International Edition, 46(9), 1455-1457. doi:10.1002/anie.200603404Chen, L., Di, J., Cao, C., Zhao, Y., Ma, Y., Luo, J., … Jiang, L. (2011). A pH-driven DNA nanoswitch for responsive controlled release. Chemical Communications, 47(10), 2850. doi:10.1039/c0cc04765aCliment, E., Bernardos, A., Martínez-Máñez, R., Maquieira, A., Marcos, M. D., Pastor-Navarro, N., … Amorós, P. (2009). Controlled Delivery Systems Using Antibody-Capped Mesoporous Nanocontainers. Journal of the American Chemical Society, 131(39), 14075-14080. doi:10.1021/ja904456dCliment, E., Martínez-Máñez, R., Sancenón, F., Marcos, M. D., Soto, J., Maquieira, A., & Amorós, P. (2010). Controlled Delivery Using Oligonucleotide-Capped Mesoporous Silica Nanoparticles. Angewandte Chemie, 122(40), 7439-7441. doi:10.1002/ange.201001847Climent, E., Martínez-Máñez, R., Sancenón, F., Marcos, M. D., Soto, J., Maquieira, A., & Amorós, P. (2010). Controlled Delivery Using Oligonucleotide-Capped Mesoporous Silica Nanoparticles. Angewandte Chemie International Edition, 49(40), 7281-7283. doi:10.1002/anie.201001847Patel, K., Angelos, S., Dichtel, W. R., Coskun, A., Yang, Y.-W., Zink, J. I., & Stoddart, J. F. (2008). Enzyme-Responsive Snap-Top Covered Silica Nanocontainers. Journal of the American Chemical Society, 130(8), 2382-2383. doi:10.1021/ja0772086Schlossbauer, A., Kecht, J., & Bein, T. (2009). Biotin-Avidin as a Protease-Responsive Cap System for Controlled Guest Release from Colloidal Mesoporous Silica. Angewandte Chemie, 121(17), 3138-3141. doi:10.1002/ange.200805818Schlossbauer, A., Kecht, J., & Bein, T. (2009). Biotin-Avidin as a Protease-Responsive Cap System for Controlled Guest Release from Colloidal Mesoporous Silica. Angewandte Chemie International Edition, 48(17), 3092-3095. doi:10.1002/anie.200805818Bernardos, A., Aznar, E., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., Soto, J., … Amorós, P. (2009). Enzyme-Responsive Controlled Release Using Mesoporous Silica Supports Capped with Lactose. Angewandte Chemie, 121(32), 5998-6001. doi:10.1002/ange.200900880Bernardos, A., Aznar, E., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., Soto, J., … Amorós, P. (2009). Enzyme-Responsive Controlled Release Using Mesoporous Silica Supports Capped with Lactose. Angewandte Chemie International Edition, 48(32), 5884-5887. doi:10.1002/anie.200900880Bernardos, A., Mondragón, L., Aznar, E., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., … Amorós, P. (2010). Enzyme-Responsive Intracellular Controlled Release Using Nanometric Silica Mesoporous Supports Capped with «Saccharides». ACS Nano, 4(11), 6353-6368. doi:10.1021/nn101499dPark, C., Kim, H., Kim, S., & Kim, C. (2009). Enzyme Responsive Nanocontainers with Cyclodextrin Gatekeepers and Synergistic Effects in Release of Guests. Journal of the American Chemical Society, 131(46), 16614-16615. doi:10.1021/ja9061085Thornton, P. D., & Heise, A. (2010). Highly Specific Dual Enzyme-Mediated Payload Release from Peptide-Coated Silica Particles. Journal of the American Chemical Society, 132(6), 2024-2028. doi:10.1021/ja9094439Coll, C., Mondragón, L., Martínez-Máñez, R., Sancenón, F., Marcos, M. D., Soto, J., … Pérez-Payá, E. (2011). Enzyme-Mediated Controlled Release Systems by Anchoring Peptide Sequences on Mesoporous Silica Supports. Angewandte Chemie, 123(9), 2186-2188. doi:10.1002/ange.201004133Coll, C., Mondragón, L., Martínez-Máñez, R., Sancenón, F., Marcos, M. D., Soto, J., … Pérez-Payá, E. (2011). Enzyme-Mediated Controlled Release Systems by Anchoring Peptide Sequences on Mesoporous Silica Supports. Angewandte Chemie International Edition, 50(9), 2138-2140. doi:10.1002/anie.201004133Cabrera, S., El Haskouri, J., Guillem, C., Latorre, J., Beltrán-Porter, A., Beltrán-Porter, D., … Amorós *, P. (2000). Generalised syntheses of ordered mesoporous oxides: the atrane route. Solid State Sciences, 2(4), 405-420. doi:10.1016/s1293-2558(00)00152-7Goldcamp, M. J., Rosa, D. T., Landers, N. A., Mandel, S. M., Krause Bauer, J. A., & Baldwin, M. J. (2000). Facile and Versatile Synthesis of Polydentate Metal Chelators with Both Amide and Oxime Donor Groups. Synthesis, 2000(14), 2033-2038. doi:10.1055/s-2000-8724Felix, F., Ferguson, J., Guedel, H. U., & Ludi, A. (1980). The electronic spectrum of tris(2,2’-bipyridine)ruthenium(2+). Journal of the American Chemical Society, 102(12), 4096-4102. doi:10.1021/ja00532a019Lytle, F. E., & Hercules, D. M. (1969). Luminescence of tris(2,2’-bipyridine)ruthenium(II) dichloride. Journal of the American Chemical Society, 91(2), 253-257. doi:10.1021/ja01030a00

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