The Yin-Yang of the Green Fluorescent Protein:Impact on Saccharomyces cerevisiae stress resistance

International audienceAlthough fluorescent proteins are widely used as biomarkers (Yin), no study focuses on their influence on the microbial stress response. Here, the Green Fluorescent Protein (GFP) was fused to two proteins of interest in Saccharomyces cerevisiae. Pab1p and Sur7p, respectively involved in stress granules structure and in Can1 membrane domains. These were chosen since questions remain regarding the understanding of the behavior of S. cerevisiae facing different heat kinetics or oxidative stresses. The main results showed that Pab1p-GFP fluorescent mutant displayed a higher resistance than that of the wild type under a heat shock. Moreover, fluorescent mutants exposed to oxidative stresses displayed changes in the cultivability compared to the wild type strain. In silico approaches showed that the presence of the GFP did not influence the structure and so the functionality of the tagged proteins meaning that changes in yeast resistance were certainly related to GFP ROS-scavenging ability (Yang)

Flow of partially molten crust controlling construction, growth and collapse of the Variscan orogenic belt: 1 the geologic record of the French Massif Central

We present here a tectonic-geodynamic model for the generation and flow of partially molten rocks and for magmatism during the Variscan orogenic evolution from the Silurian to the late Carboniferous based on a synthesis of geological data from the French Massif Central. Eclogite facies metamorphism of mafic and ultramafic rocks records the subduction of the Gondwana hyperextended margin. Part of these eclogites are forming boudins-enclaves in felsic HP granulite facies migmatites partly retrogressed into amphibolite facies attesting for continental subduction followed by thermal relaxation and decompression. We propose that HP partial melting has triggered mechanical decoupling of the partially molten continental rocks from the subducting slab. This would have allowed buoyancy-driven exhumation and entrainment of pieces of oceanic lithosphere and subcontinental mantle. Geochronological data of the eclogite-bearing HP migmatites points to diachronous emplacement of distinct nappes from middle to late Devonian. These nappes were thrusted onto metapelites and orthogneisses affected by MP/MT greenschist to amphibolite facies metamorphism reaching partial melting attributed to the late Devonian to early Carboniferous thickening of the crust. The emplacement of laccoliths rooted into strike-slip transcurrent shear zones capped by low-angle detachments from c. 345 to c. 310 Ma is concomitant with the southward propagation of the Variscan deformation front marked by deposition of clastic sediments in foreland basins. We attribute these features to horizontal growth of the Variscan belt and formation of an orogenic plateau by gravity-driven lateral flow of the partially molten orogenic root. The diversity of the magmatic rocks points to various crustal sources with modest, but systematic mantle-derived input. In the eastern French Massif Central, the southward decrease in age of the mantle- and crustal-derived plutonic rocks from c. 345 Ma to c. 310 Ma suggests southward retreat of a northward subducting slab toward the Paleotethys free boundary. Late Carboniferous destruction of the Variscan belt is dominantly achieved by gravitational collapse accommodated by the activation of low-angle detachments and the exhumation-crystallization of the partially molten orogenic root forming crustal-scale LP migmatite domes from c. 305 Ma to c. 295 Ma, coeval with orogen-parallel flow in the external zone. Laccoliths emplaced along low-angle detachments and intrusive dykes with sharp contacts correspond to the segregation of the last melt fraction leaving behind a thick accumulation of refractory LP felsic and mafic granulites in the lower crust. This model points to the primordial role of partial melting and magmatism in the tectonic-geodynamic evolution of the Variscan orogenic belt. In particular, partial melting and magma transfer (i) triggers mechanical decoupling of subducted units from the downgoing slab and their syn-orogenic exhumation; (ii) the development of an orogenic plateau by lateral flow of the low-viscosity partially molten crust; and, (iii) the formation of metamorphic core complexes and domes that accommodate post-orogenic exhumation during gravitational collapse. All these processes contribute to differentiation and stabilisation of the orogenic crust

Production d'anticorps monoclonaux in vitro, mise au point d'un milieu de culture synthetique et selection d'un procede

SIGLEAvailable from INIST (FR), Document Supply Service, under shelf-number : T 82969 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Accounting and reimbursement schemes for inpatient care in France

International audienceThe new French case-mix system of hospital payment was adopted in 2004 for public hospitals and in March 2005 for private-for-profit hospitals. Implementing this reform requires a period of transition but the challenges ahead can already be predicted. Prices will have to change before this mode of reimbursement can have any real impact. This requires producing more detailed hospital cost data and using fine measuring tools such as the cost accounting method developed for use in this context. This article describes and analyses the main tools and methods selected to implement the new French prospective payment system

Accounting and reimbursement schemes for inpatient care in France

The new French case-mix system of hospital payment was adopted in 2004 for public hospitals and in March 2005 for private-for-profit hospitals. Implementing this reform requires a period of transition but the challenges ahead can already be predicted. Prices will have to change before this mode of reimbursement can have any real impact. This requires producing more detailed hospital cost data and using fine measuring tools such as the cost accounting method developed for use in this context. This article describes and analyses the main tools and methods selected to implement the new French prospective payment system. Copyright Springer Science + Business Media, LLC 2006Cost accounting methodology, Tariffs, Hospital costs, Prospective payment system, France,

1919, les quatre saisons de l’urbanisme- Introduction. Un après-guerre politique et urbanistique

https://www.inventerlegrandparis.fr/link/?id=703Emmanuel Bellanger Suresnes est une ville emblématique dans l'histoire des banlieues et du Grand Paris. Elle est incarnée par Henri Sellier figure incontournable de l’histoire urbaine, sociale et politique de l’entre-deux-guerres. Cette journée élaborée en partenariat avec le Musée d'histoire urbaine et sociale de Suresnes en témoigne (MUS). Suresnes est associée au nom d’Henri Sellier, maire de cette commune de 1919 à 1941, animé d'une passion pour les villes et singulièrement pour la sienne. Henri Sellier est porté par une expérience édilitaire et un idéal militant, révolutionnaire et réformiste à la fois. Son parcours est caractérisé par une action publique..

Heart signal recognition by Hidden Markov Models: the ECG case.

International audienceWave recognition in ECG signals by Hidden Markov Models (HMMs) relies on the stationary assumption for the set of parameters used to describe ECG waves. This approach seems unnatural and consequently generates severe errors in practice. A new class of HMMs called Modified Continuous Variable Duration HMMs is proposed to account for the specific properties of the ECG signal. An application of the latter, coupled with a multiresolution front-end analysis of the ECG is presented. Results show these methods can increase the performance of ECG recognition compared to classical HMMs

Are Crustal Fault Zones viable geothermal resources? Insights from the Pontgibaud Fault in French Massif Central

International audienceNumerous recent studies indicate that crustal-scale fault zones represent efficient conduits for meteoric fluid to flow down to mid-crustal depths (Haines et al., 2016), in particular near the brittle-ductile transition where temperature are around 350-450°C (Violay et al., 2016). The present study aims to understand the potential of a new and novel type of geothermal system for high temperature and electricity production: the Crustal Fault Zones (CFZ). The geothermal potential estimation in a basement context requires combination of geological, geophysical and thermal data. The Pontgibaud fault zone (Puy-de-Dôme, French Massif Central) includes an intensive multivariate dataset