Transcription specificity of Xenopus laevis RNA polymerase A

Based on a combination of an Ugi four component reaction and a ring closing metathesis, a library of novel artificial macrocyclic inhibitors of the p53-MDM2 interaction was designed and synthesized. These macrocycles, alternatively to stapled peptides, target for the first time the large hydrophobic surface area formed by Tyr67, Gln72, His73, Val93, and Lys94 yielding derivatives with affinity to MDM2 in the nanomolar range. Their binding affinity with MDM2 was evaluated using fluorescence polarization (FP) assay and 1H-15N two-dimensional HSQC nuclear magnetic resonance experiments

Design of strapdown gyroscopes for a dynamic environment Semiannual report, 1 Dec. 1968 - 31 May 1969

Strapdown gyros which employ time modulation torquing techniqu

Literary Ethics

Review of 1997-98 books by Rey Chow, Thomas Keenan, Colin McGin

Design of strapdown gyroscopes for a dynamic environment Semiannual report, Dec. 1967 - May 1968

Systems analysis, design, and operating characteristics of strapdown gyroscopes for dynamic environmen

Conservation practice could benefit from routine testing and publication of management outcomes

Effective conservation requires a step change in the way practitioners can contribute to science and can have access to research outputs. The journal Conservation Evidence was established in 2004 to help practitioners surmount several obstacles they face when attempting to document the effects of their conservation actions scientifically. It is easily and freely accessible online. It is free to publish in and it enables global communication of the effects of practical trials and experiments, which are virtually impossible to get published in most scientific journals. The driving force behind Conservation Evidence is the need to generate and share scientific information about the effects of interventions

Developmental toxicity of bisphenol A diglycidyl ether (epoxide resin badge) during the early life cycle of a native amphibian species

Bisphenol A diglycidyl ether (BADGE) is used in packaging materials, in epoxy adhesives, and as an additive for plastics, but it is also a potential industrial wastewater contaminant. The aim of the present study was to evaluate the adverse effects of BADGE on Rhinella arenarum by means of standardized bioassays at embryo–larval development. The results showed that BADGE was more toxic to embryos than to larvae at all exposure times. At acute exposure, lethality rates of embryos exposed to concentrations of 0.0005 mg/L BADGE and greater were significantly higher than rates in the vehicle control, whereas lethality rates of larvae were significantly higher in concentrations of 10 mg/L BADGE and greater. The toxicity then increased significantly, with 96‐h median lethal concentrations (LC50s) of 0.13 mg/L and 6.9 mg/L BADGE for embryos and larvae, respectively. By the end of the chronic period, the 336‐h LC50s were 0.04 mg/L and 2.2 mg/L BADGE for embryos and larvae, respectively. This differential sensitivity was also ascertained by the 24‐h pulse exposure experiments, in which embryos showed a stage‐dependent toxicity, with blastula being the most sensitive stage and S.23 the most resistant. The most important sublethal effects in embryos were cell dissociation and delayed development, whereas the main abnormalities observed in larvae related to neurotoxicity, as scare response to stimuli and narcotic effect.Fil: Hutler Wolkowicz, Ianina Ruth. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Svartz, Gabriela Veronica. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aronzon, Carolina Mariel. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez Coll, Cristina Silvia. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Genetic Divergence for the Amphibian Pleurodeles waltl in Southwest Portugal: Dispersal Barriers Shaping Geographic Patterns

Dispersal and vicariance are the principal mechanisms responsible for the formation of biogeographic patterns, driven or maintained by the role of past and current barriers to dispersal. Southwest Portugal harbors a rich endemic fauna and flora where strongly differentiated taxa can be observed, suggesting ancient isolating mechanisms acted in this region. In this study, we used information from two partial mitochondrial sequences, combined with information from microsatellite loci and amplification success, to investigate biogeographic patterns of genetic divergence in populations of Pleurodeles waltl in Portugal. Our results demonstrate genetic differentiation at different time frames creating distinct populations in southwest Portugal, which might have arisen following isolation by a large river estuary and mountainous barriers. Habitat destruction associated with agricultural intensification raises conservation concerns over the unique biodiversity in this region.Fundacao para a Ciencia e Tecnologia (Portugal); FEDER [PTDC/BIA-BDE/68730/2006, SFRH/BD/24064/2005