Study Results of the e-Learning Readiness of Mongolian Students

While the pandemic has increased the use of e-learning in education, access varies by location. In order to determine what kind of policy support is needed to support e-learning in the future, it was found that the readiness of students for e-learning is at average level. The purpose of this study is the correlation between the e-learning readiness and technical skills, the ability to communicate with the teacher, the ability to communicate with colleagues, and the communication skills of our country's high school students using an international survey questionnaire. Research analysis were done using SPSS. According to the study results, the location of the schools, the age and gender of the students do not affect the e-learning readiness. However, the fact that the student has his own computer and has sufficient Internet access affects the readiness of e-learning. According to the correlation analysis, technical competence, communication competence with the teacher, communication competence with colleagues, and communication competence are positively related to each other. Also, a survey of students' e-readiness was taken from the teacher, and a discrepancy was observed between the students and the teachers

Second extracellular loop of human glucagon-like peptide-1 receptor (GLP-1R) has a critical role in GLP-1 peptide binding and receptor activation

The glucagon-like peptide-1 receptor (GLP-1R) is a therapeutically important family B G protein-coupled receptor (GPCR) that is pleiotropically coupled to multiple signaling effectors and, with actions including regulation of insulin biosynthesis and secretion, is one of the key targets in the management of type II diabetes mellitus. However, there is limited understanding of the role of the receptor core in orthosteric ligand binding and biological activity. To assess involvement of the extracellular loop (ECL) 2 in ligand-receptor interactions and receptor activation, we performed alanine scanning mutagenesis of loop residues and assessed the impact on receptor expression and GLP-1(1-36)-NH 2 or GLP-1(7-36)-NH2 binding and activation of three physiologically relevant signaling pathways as follows: cAMP formation, intracellular Ca 2+ (Ca 2+ i) mobilization, and phosphorylation of extracellular signal-regulated kinases 1 and 2 (pERK1/2). Although antagonist peptide binding was unaltered, almost all mutations affected GLP-1 peptide agonist binding and/or coupling efficacy, indicating an important role in receptor activation. However, mutation of several residues displayed distinct pathway responses with respect to wild type receptor, including Arg-299 and Tyr-305, where mutation significantly enhanced both GLP-1(1-36)-NH2- and GLP-1(7-36)- NH 2-mediated signaling bias for pERK1/2. In addition, mutation of Cys-296, Trp-297, Asn-300, Asn-302, and Leu-307 significantly increased GLP-1(7-36)-NH 2-mediated signaling bias toward pERK1/2. Of all mutants studied, only mutation of Trp-306 to alanine abolished all biological activity. These data suggest a critical role of ECL2 of the GLP-1R in the activation transition(s) of the receptor and the importance of this region in the determination of both GLP-1 peptide- and pathway-specific effects

Abschlussbericht des Projekts SiLEST (Software in teh Loop for Embedded Software Test) in der BMBF-Forschungsoffensive "Software Engineering 2006"

Der Bericht beschreibt einen Testprozess für die Durchführung von Model-, Software-, Processor- und Hardware-in-the-Loop-Tests mit der Möglichkeit der Fehlerinjektion. Der Testprozess ist weitestgehend automatisiert und das Werkzeug zur Testautomatisierung ist offen für die Einbindung in unterschiedliche Testinfrastrukturen und Testmanagementsystemen. Ein besonderes Augenmerk wurde dabei auf die Wiederverwendung von Fehlerfällen und Simulationsumgebungen gelegt. Der Testprozess wurde exemplarisch an einem Motormanagementsystem und einem Satellitenonboardsystem erprobt und bewertet

Hexa-histidin tag position influences disulfide structure but not binding behavior of in vitro folded N-terminal domain of rat corticotropin-releasing factor receptor type 2a

The oxidative folding, particularly the arrangement of disulfide bonds of recombinant extracellular N-terminal domains of the corticotropin-releasing factor receptor type 2a bearing five cysteines (C2 to C6), was investigated. Depending on the position of a His-tag, two types of disulfide patterns were found. In the case of an N-terminal His-tag, the disulfide bonds C2–C3 and C4–C6 were found, leaving C5 free, whereas the C-terminal position of the His-tag led to the disulfide pattern C2–C5 and C4–C6, and leaving C3 free. The latter pattern is consistent with the disulfide arrangement of the extracellular N-terminal domain of the corticotropin-releasing factor (CRF) receptor type 1, which has six cysteines (C1 to C6) and in which C1 is paired with C3. However, binding data of the two differently disulfide-bridged domains show no significant differences in binding affinities to selected ligands, indicating the importance of the C-terminal portion of the N-terminal receptor domains, particularly the disulfide bond C4–C6 for ligand binding