1,113 research outputs found
Epitaxial Growth of LaSrFeO thin films by laser ablation
We report on the synthesis of high quality LaSrFeO (LSFO)
thin films using the pulsed laser deposition technique on both SrTiO (STO)
and LaAlO (LAO) substrates (100)-oriented. From X-Ray diffraction (XRD)
studies, we find that the films have an out-of-plane lattice parameter around
0.3865nm, almost independent of the substrate (i.e. the nature of the strains).
The transport properties reveal that, while LSFO films deposited on STO exhibit
an anomaly in the resistivity vs temperature at 180K (corresponding to the
charge-ordered transition and associated with a transition from a paramagnetic
to an antiferromagnetic state), the films grown on LAO display a very small
magnetoresistance behavior and present an hysteresis around 270K under the
application of a 4T magnetic field. The changes in transport properties between
both substrates are discussed and compared with the corresponding single
crystals.Comment: 9 pages, 4 figure
Motif Discovery through Predictive Modeling of Gene Regulation
We present MEDUSA, an integrative method for learning motif models of
transcription factor binding sites by incorporating promoter sequence and gene
expression data. We use a modern large-margin machine learning approach, based
on boosting, to enable feature selection from the high-dimensional search space
of candidate binding sequences while avoiding overfitting. At each iteration of
the algorithm, MEDUSA builds a motif model whose presence in the promoter
region of a gene, coupled with activity of a regulator in an experiment, is
predictive of differential expression. In this way, we learn motifs that are
functional and predictive of regulatory response rather than motifs that are
simply overrepresented in promoter sequences. Moreover, MEDUSA produces a model
of the transcriptional control logic that can predict the expression of any
gene in the organism, given the sequence of the promoter region of the target
gene and the expression state of a set of known or putative transcription
factors and signaling molecules. Each motif model is either a -length
sequence, a dimer, or a PSSM that is built by agglomerative probabilistic
clustering of sequences with similar boosting loss. By applying MEDUSA to a set
of environmental stress response expression data in yeast, we learn motifs
whose ability to predict differential expression of target genes outperforms
motifs from the TRANSFAC dataset and from a previously published candidate set
of PSSMs. We also show that MEDUSA retrieves many experimentally confirmed
binding sites associated with environmental stress response from the
literature.Comment: RECOMB 200
Coulomb correlation and magnetic ordering in double-layered manganites: LaSrMnO
A detailed study of the electronic structure and magnetic configurations of
the 50 % hole-doped double layered manganite LaSrMnO is presented.
We demonstrate that the on-site Coulomb correlation (U) of Mn d electrons {\it
(i)} significantly modifies the electronic structure, magnetic ordering (from
FM to AFM), and interlayer exchange interactions, and {\it (ii)} promotes
strong anisotropy in electrical transport, reducing the effective hopping
parameter along the {\it c} axis for electrically active electrons. This
findng is consistent with observations of anisotropic transport -- a property
which sets this manganite apart from conventional 3D systems. A half-metallic
band structure is predicted with both the LSDA and LSDA+U methods. The
experimentally observed A-type AFM ordering in LaSrMnO is found to
be energetically more favorable with U 7 eV. A simple interpretation of
interlayer exchange coupling is given within double and super-exchange
mechanisms based on the dependencies on U of the effective exchange parameters
and state sub-band widths.Comment: 10 pages, 5 figure
Orbital Structure and Magnetic Ordering in Layered Manganites: Universal Correlation and Its Mechanism
Correlation between orbital structure and magnetic ordering in bilayered
manganites is examined. A level separation between the and
orbitals in a Mn ion is calculated in the ionic model for a
large number of the compounds. It is found that the relative stability of the
orbitals dominates the magnetic transition temperatures as well as the magnetic
structures. A mechanism of the correlation between orbital and magnetism is
investigated based on the theoretical model with the two orbitals under
strong electron correlation.Comment: 4 pages, 4 figure
Solid-phase extraction and purification of membrane proteins using a UV-modified PMMA microfluidic bioaffinity mu SPE device
We present a novel microfluidic solid-phase extraction (??SPE) device for the affinity enrichment of biotinylated membrane proteins from whole cell lysates. The device offers features that address challenges currently associated with the extraction and purification of membrane proteins from whole cell lysates, including the ability to release the enriched membrane protein fraction from the extraction surface so that they are available for downstream processing. The extraction bed was fabricated in PMMA using hot embossing and was comprised of 3600 micropillars. Activation of the PMMA micropillars by UV/O3 treatment permitted generation of surface-confined carboxylic acid groups and the covalent attachment of NeutrAvidin onto the ??SPE device surfaces, which was used to affinity select biotinylated MCF-7 membrane proteins directly from whole cell lysates. The inclusion of a disulfide linker within the biotin moiety permitted release of the isolated membrane proteins via DTT incubation. Very low levels (???20 fmol) of membrane proteins could be isolated and recovered with ???89% efficiency with a bed capacity of 1.7 pmol. Western blotting indicated no traces of cytosolic proteins in the membrane protein fraction as compared to significant contamination using a commercial detergent-based method. We highlight future avenues for enhanced extraction efficiency and increased dynamic range of the ??SPE device using computational simulations of different micropillar geometries to guide future device designs.close2
Charge Ordering and Phase Competition in the Layered Perovskite Lasr2mn2o7
Charge-lattice fluctuations are observed in the layered perovskite manganite
LaSr2Mn2O7 by Raman spectroscopy as high as 340 K and with decreasing
temperature they become static and form a charge ordered (CO) phase below
TCO=210 K. In the static regime, superlattice reflections are observed through
neutron and x-ray diffraction with a propagation vector (h+1/4,k-1/4,l).
Crystallographic analysis of the CO state demonstrates that the degree of
charge and orbital ordering in this manganite is weaker than the charge
ordering in three dimensional perovskite manganites. A TN=170K a type-A
antiferromagnetism (AF) develops and competes with the charge ordering, that
eventually melts below T*=100K. High resolution diffraction measurements
suggest that that CO- and AF-states do not coincide within the same region in
the material but rather co-exist as separate phases. The transition to type-A
antiferromagnetism at lower temperatures is characterized by the competition
between these two phases.Comment: 9 pages, 6 figure
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