60 research outputs found
Acyl-Imidazoles A Privileged Ester Surrogate for Enantioselective Synthesis
International audienceSince the first report by Evans in asymmetric FriedelâCrafts reactions, the use of acylâimidazoles has blossomed as powerful ester/amide surrogates. The imidazole scaffold indeed displays stability and special activation features allowing both better reactivity and selectivity in traditional ester/amide functionalizations: αâ(enolate chemistry), ÎČâ(conjugate additions), α,ÎČâ(cycloadditions) or Îł/ÎŽâ(vinylogous). An overview of the contemporary and growing interest in acylâimidazoles in metalâ and organoâcatalyzed transformations (bioâhybrid catalytic systems will be fully described in a backâtoâback Minireview) will be highlighted. Moreover, postâfunctionalization expediencies are also going to be discussed in this Minireview
Structure and assembly of the S-layer in C. difficile
Many bacteria and archaea possess a two-dimensional protein array, or S-layer, that covers the cell surface and plays crucial roles in cell physiology. Here, we report the crystal structure of SlpA, the main S-layer protein of the bacterial pathogen Clostridioides difficile, and use electron microscopy to study S-layer organisation and assembly. The SlpA crystal lattice mimics S-layer assembly in the cell, through tiling of triangular prisms above the cell wall, interlocked by distinct ridges facing the environment. Strikingly, the array is very compact, with pores of only ~10âĂ
in diameter, compared to other S-layers (30â100âĂ
). The surface-exposed flexible ridges are partially dispensable for overall structure and assembly, although a mutant lacking this region becomes susceptible to lysozyme, an important molecule in host defence. Thus, our work gives insights into S-layer organisation and provides a basis for development of C. difficile-specific therapeutics
Reactions Catalysed by a Binuclear Copper Complex: Aerobic Cross Dehydrogenative Coupling of N-Aryl Tetrahydroisoquinolines
A hybrid bioorganic interface for neuronal photoactivatio
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