Household Water Supply Strategies in Urban Bandung, Indonesia: Findings and Implications for Future Water Access Reporting

Through structured interviews and statistical analyses, this study investigated access to water and strategies of 1227 vulnerable households in Bandung, Indonesia. The use of mixed water sources, household water treatment, and home storage suggest low trust in improved sources, and compromised safety and reliability of water. While official statistics suggest a high level of access to improved water sources, full-time access to such sources is overestimated. Integration of user behavior into the new monitoring approach for the water supply sector in the post-2015 development framework is proposed

Temporal and spatial patterns of bovine Escherichia coli O157 prevalence and comparison of temporal changes in the patterns of phage types associated with bovine shedding and human E. coli O157 cases in Scotland between 1998-2000 and 2002-2004

Background: Escherichia coli O157 is an important cause of acute diarrhoea, haemorrhagic colitis and, especially in children, haemolytic uraemic syndrome (HUS). Incidence rates for human E. coli O157 infection in Scotland are higher than most other United Kingdom, European and North American countries. Cattle are considered the main reservoir for E. coli O157. Significant associations between livestock related exposures and human infection have been identified in a number of studies. Results: Animal Studies: There were no statistically significant differences (P = 0.831) in the mean farm-level prevalence between the two studies (SEERAD: 0.218 (95% CI: 0.141-0.32); IPRAVE: 0.205 (95% CI: 0.135-0.296)). However, the mean pat-level prevalence decreased from 0.089 (95% CI: 0.075-0.105) to 0.040 (95% CI: 0.028-0.053) between the SEERAD and IPRAVE studies respectively (P < 0.001). Highly significant (P < 0.001) reductions in mean pat-level prevalence were also observed in the spring, in the North East and Central Scotland, and in the shedding of phage type (PT) 21/28. Human Cases: Contrasting the same time periods, there was a decline in the overall comparative annual reported incidence of human cases as well as in all the major PT groups except 'Other' PTs. For both cattle and humans, the predominant phage type between 1998 and 2004 was PT21/28 comprising over 50% of the positive cattle isolates and reported human cases respectively. The proportion of PT32, however, was represented by few (<5%) of reported human cases despite comprising over 10% of cattle isolates. Across the two studies there were differences in the proportion of PTs 21/28, 32 and 'Other' PTs in both cattle isolates and reported human cases; however, only differences in the cattle isolates were statistically significant (P = 0.002). Conclusion: There was no significant decrease in the mean farm-level prevalence of E. coli O157 between 1998 and 2004 in Scotland, despite significant declines in mean pat-level prevalence. Although there were declines in the number of human cases between the two study periods, there is no statistically significant evidence that the overall rate (per 100,000 population) of human E. coli O157 infections in Scotland over the last 10 years has altered. Comparable patterns in the distribution of PTs 21/28 and 32 between cattle and humans support a hypothesized link between the bovine reservoir and human infections. This emphasizes the need to apply and improve methods to reduce bovine shedding of E. coli O157 in Scotland where rates appear higher in both cattle and human populations, than in other countrie

PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells

Immunolabeling and autoradiography have traditionally been applied as the methods-of-choice to visualize and collect molecular information about physiological and pathological processes. Here, we introduce PharmacoSTORM super-resolution imaging that combines the complementary advantages of these approaches and enables cell-type- and compartment-specific nanoscale molecular measurements. We exploited rational chemical design for fluorophore-tagged high-affinity receptor ligands and an enzyme inhibitor; and demonstrated broad PharmacoSTORM applicability for three protein classes and for cariprazine, a clinically approved antipsychotic and antidepressant drug. Because the neurobiological substrate of cariprazine has remained elusive, we took advantage of PharmacoSTORM to provide in vivo evidence that cariprazine predominantly binds to D3 dopamine receptors on Islands of Calleja granule cell axons but avoids dopaminergic terminals. These findings show that PharmacoSTORM helps to quantify drug-target interaction sites at the nanoscale level in a cell-type- and subcellular context-dependent manner and within complex tissue preparations. Moreover, the results highlight the underappreciated neuropsychiatric significance of the Islands of Calleja in the ventral forebrain.NWO724.017.002Molecular Physiolog

Endothelin-1 as a neuropeptide: neurotransmitter or neurovascular effects?

Endothelin-1 (ET-1) is an endothelium-derived peptide that also possesses potent mitogenic activity. There is also a suggestion the ET-1 is a neuropeptide, based mainly on its histological identification in both the central and peripheral nervous system in a number of species, including man. A neuropeptide role for ET-1 is supported by studies showing a variety of effects caused following its administration into different regions of the brain and by application to peripheral nerves. In addition there are studies proposing that ET-1 is implicated in a number of neural circuits where its transmitter affects range from a role in pain and temperature control to its action on the hypothalamo-neurosecretory system. While the effect of ET-1 on nerve tissue is beyond doubt, its action on nerve blood flow is often ignored. Here, we review data generated in a number of species and using a variety of experimental models. Studies range from those showing the distribution of ET-1 and its receptors in nerve tissue to those describing numerous neurally-mediated effects of ET-1

Dues tombes romanes a Llafranc

El descobriment de dues inhumacions acompanyades d'ofrenes que van ser recuperades i salvades, permet realitzar un estudi detallat i fixar amb precisió la data dels enterraments que ajuden a precisar la història de la necròpoli romana de Llafran

The linkage of water quality measurement the downstream of the Citarum cascade reservoir management

Cascade Citarum Reservoirs, located in the western part of the island of Java in Indonesia country, cover the main building dams, hydropower, irrigation facilities and other supporting facilities. The study aimed to evaluate the characteristics of the existing condition of the downstream reservoir, including the relation of the water quality and quantity, by using a conceptual model of the Citarum Cascade Reservoir management. The study mostly used the secondary data acquired from Indonesia Power, the Management Bureau of Cirata Dam (BPWC), Perum Jasa Tirta II (PJT) Jatiluhur, the Management Bureau of the Citarum-Ciliwung River Basin (BPDAS Citarum-Ciliwung), and the Water Resource Management Agency of the West Java Province. This research aims to predict the discharge plans R5 and R20 using the Weibull method and the uncertainty of future discharge using the continuous model approach. Furthermore, the multivariate statistical test will analyse the linkage of water quality in reservoir management. To solve the problem of the allocation of the raw water discharge to fulfil the water requirement in the downstream area was developed proportionally in this study, in which the raw water discharge allocations were 46.92 m3/sec, 91.9 m3 /sec and 97.95 m3/sec for Saguling, Cirata, and Jatiluhur, respectively. The tests to determine the relationship between the discharge and water quality using the Discriminant test indicated that the discharge correlates strongly with the parameters such as NH3. BOD and CO