907 research outputs found
A spectroscopic and thermal investigation into the relationship between composition, secondary structure and physical characteristics of electrospun zein nanofibers
Electrospun zein nanofibers have attracted interest as drug delivery systems due to their propensity for controlled drug release, flexible structure and low toxicity. However, comparatively little is known regarding the relationship between production method and fiber characteristics, both in terms of fiber architecture and protein structure. Here we use a range of imaging and spectroscopic techniques to elucidate the effects of solvent composition on zein secondary structure, fiber diameter and fiber integrity, plus we utilize the new technique of transition temperature microscopy to examine the thermal properties of the fibers. Zein nanofibers were prepared using ethanol, acetic acid and water mixes as solvents, alone and with plasticizers (polyethylene glycol, glycerol) and casein. Electrospinning was performed under controlled conditions and the products characterized using scanning electron microscopy (SEM), attenuated total reflection Fourier Transform infrared spectrometry (ATR - FTIR) and transition temperature microscopy (TTM). The choice of solvent, concentration and voltage, alongside the presence of additives (plasticizers and casein) were noted to influence both the diameter of the fibers and the tendency for bead formation. A relationship was noted between protein secondary structure and fiber architecture, with an enhanced β-sheet content, enhanced by the inclusion of casein, being associated with higher beading. In addition, thermal imaging of electrospun zein fiber mats was successfully achieved using TTM via two dimensional mapping of the softening temperatures across the spun samples, in particular demonstrating the plasticizing effects of the polyethylene glycol and glycerol
Associations between sole ulcer, white line disease and digital dermatitis and the milk yield of 1824 dairy cows on 30 dairy cow farms in England and Wales from February 2003–November 2004
The milk yields of 1824 cows were used to investigate the effect of lesion-specific causes of lameness, based on farmer treatment and diagnosis of lame cows, on milk yield. A three level hierarchical model of repeated test day yields within cows within herds was used to investigate the impact of lesion-specific causes of lameness (sole ulcer, white line disease, digital dermatitis and other causes) on milk yield before and after treatment compared with unaffected cows. Cattle which developed sole ulcer (SU) and white line disease (WLD) were higher yielding cattle before they were diagnosed. Their milk production fell to below that of the mean of unaffected cows before diagnosis and remained low after diagnosis. In cattle which developed digital dermatitis (DD) there was no significant difference in milk yield before treatment and a slightly raised milk yield immediately after treatment. The estimated milk loss attributable to SU and WLD was approximately 570kg and 370kg respectively. These results highlight that specific types of lameness vary by herds and within herds they are associated with higher yielding cattle. Consequently lesion-specific lameness reduction programmes targeting the cow and farm specific causes of lameness might be more effective than generic recommendations. They also highlight the importance of milk loss when estimating the economic impact of SU and WLD on the farms profitability
On parton distributions beyond the leading order
The importance of properly taking into account the factorization scheme
dependence of parton distribution functions is emphasized. A serious error in
the usual handling of this topic is pointed out and the correct procedure for
transforming parton distribution functions from one factorisation scheme to
another recalled. It is shown that the conventional and
DIS definitions thereof are ill-defined due to the lack of distinction between
the factorisation scheme dependence of parton distribution functions and
renormalisation scheme dependence of the strong coupling constant . A
novel definition of parton distribution functions is suggested and its role in
the construction of consistent next-to-leading order event generators briefly
outlined.Comment: PRA-HEP-93/05, Latex, 10 pages and 2 Postscript figures appended at
the end of this fil
On the pion-nucleon coupling constant
In view of persisting misunderstanding about the determination of the
pion-nucleon coupling constants in the Nijmegen multienergy partial-wave
analyses of pp, np, and pbar-p scattering data, we present additional
information which may clarify several points of discussion. We comment on
several recent papers addressing the issue of the pion-nucleon coupling
constant and criticizing the Nijmegen analyses.Comment: 19 pages, Nijmegen preprint THEF-NYM-92-0
An Equation of State of a Carbon-Fibre Epoxy Composite under Shock Loading
An anisotropic equation of state (EOS) is proposed for the accurate
extrapolation of high-pressure shock Hugoniot (anisotropic and isotropic)
states to other thermodynamic (anisotropic and isotropic) states for a shocked
carbon-fibre epoxy composite (CFC) of any symmetry. The proposed EOS, using a
generalised decomposition of a stress tensor [Int. J. Plasticity \textbf{24},
140 (2008)], represents a mathematical and physical generalisation of the
Mie-Gr\"{u}neisen EOS for isotropic material and reduces to this equation in
the limit of isotropy. Although a linear relation between the generalised
anisotropic bulk shock velocity and particle velocity was
adequate in the through-thickness orientation, damage softening process
produces discontinuities both in value and slope in the -
relation. Therefore, the two-wave structure (non-linear anisotropic and
isotropic elastic waves) that accompanies damage softening process was proposed
for describing CFC behaviour under shock loading. The linear relationship
- over the range of measurements corresponding to non-linear
anisotropic elastic wave shows a value of (the intercept of the
- curve) that is in the range between first and second
generalised anisotropic bulk speed of sound [Eur. Phys. J. B \textbf{64}, 159
(2008)]. An analytical calculation showed that Hugoniot Stress Levels (HELs) in
different directions for a CFC composite subject to the two-wave structure
(non-linear anisotropic elastic and isotropic elastic waves) agree with
experimental measurements at low and at high shock intensities. The results are
presented, discussed and future studies are outlined.Comment: 12 pages, 9 figure
Microfibrous Solid Dispersions of Poorly Water-Soluble Drugs Produced via Centrifugal Spinning: Unexpected Dissolution Behavior on Recrystallization
Temperature-controlled, solvent-free centrifugal spinning may be used as a means of rapid production of amorphous solid dispersions in the form of drug-loaded sucrose microfibers. However, due to the high content of amorphous sucrose in the formulations, such microfibers may be highly hygroscopic and unstable on storage. In this study, we explore both the effects of water uptake of the microfibers and the consequences of deliberate recrystallization for the associated dissolution profiles. The stability of sucrose microfibers loaded with three selected BCS class II model drugs (itraconazole (ITZ), olanzapine (OLZ), and piroxicam (PRX)) was investigated under four different relative humidity conditions (11, 33, 53, and 75% RH) at 25 °C for 8 months, particularly focusing on the effect of the highest level of moisture (75% RH) on the morphology, size, drug distribution, physical state, and dissolution performance of microfibers. While all samples were stable at 11% RH, at 33% RH the ITZ-sucrose system showed greater resistance against devitrification compared to the OLZ- and PRX-sucrose systems. For all three samples, the freshly prepared microfibers showed enhanced dissolution and supersaturation compared to the drug alone and physical mixes; surprisingly, the dissolution advantage was largely maintained or even enhanced (in the case of ITZ) following the moisture-induced recrystallization under 75% RH. Therefore, this study suggests that the moisture-induced recrystallization process may result in considerable dissolution enhancement compared to the drug alone, while overcoming the physical stability risks associated with the amorphous state
Profiling social, emotional and behavioural difficulties of children involved in direct and indirect bullying behaviours
Being involved in bullying places a child at risk of poor psychosocial and educational outcomes. This study aimed to examine the profile of behavioural, emotional and social functioning for two subtypes of bullying; direct and indirect (relational). Pupils aged between seven and eleven years old completed sociometric measures of social inclusion and bullying behaviour to identify 192 pupils considered to be involved in either direct, indirect, both or neither types of bullying. These pupils and their teachers completed a battery of assessments relating to behaviour, social competence and self-perception. All bully-groups experienced similar levels of significant social rejection. ‘Direct’ and ‘both’ groups showed the greatest number of behavioural, emotional and social difficulties, while the ‘indirect’ group showed weaknesses in self-perception, but no teacher-rated problems. Understanding the behavioural, emotional and social correlates of bullying is of particular importance for early identification of children at risk of becoming bullies and for developing targeted interventions
New Upper Limit of Terrestrial Equivalence Principle Test for Rotating Extended Bodies
Improved terrestrial experiment to test the equivalence principle for
rotating extended bodies is presented, and a new upper limit for the violation
of the equivalence principle is obtained at the level of 1.6, which is limited by the friction of the rotating gyroscope. It
means the spin-gravity interaction between the extended bodies has not been
observed at this level.Comment: 4 page
Cross-talk between GABAergic postsynapse and microglia regulate synapse loss after brain ischemia.
Microglia interact with neurons to facilitate synapse plasticity; however, signal(s) contributing to microglia activation for synapse elimination in pathology are not fully understood. Here, using in vitro organotypic hippocampal slice cultures and transient middle cerebral artery occlusion (MCAO) in genetically engineered mice in vivo, we report that at 24 hours after ischemia, microglia release brain-derived neurotrophic factor (BDNF) to downregulate glutamatergic and GABAergic synapses within the peri-infarct area. Analysis of the cornu ammonis 1 (CA1) in vitro shows that proBDNF and mBDNF downregulate glutamatergic dendritic spines and gephyrin scaffold stability through p75 neurotrophin receptor (p75 <sup>NTR</sup> ) and tropomyosin receptor kinase B (TrkB) receptors, respectively. After MCAO, we report that in the peri-infarct area and in the corresponding contralateral hemisphere, similar neuroplasticity occurs through microglia activation and gephyrin phosphorylation at serine-268 and serine-270 in vivo. Targeted deletion of the Bdnf gene in microglia or GphnS268A/S270A (phospho-null) point mutations protects against ischemic brain damage, neuroinflammation, and synapse downregulation after MCAO
Search for the Rare Decay KL --> pi0 ee
The KTeV/E799 experiment at Fermilab has searched for the rare kaon decay
KL--> pi0ee. This mode is expected to have a significant CP violating
component. The measurement of its branching ratio could support the Standard
Model or could indicate the existence of new physics. This letter reports new
results from the 1999-2000 data set. One event is observed with an expected
background at 0.99 +/- 0.35 events. We set a limit on the branching ratio of
3.5 x 10^(-10) at the 90% confidence level. Combining the results with the
dataset taken in 1997 yields the final KTeV result: BR(KL --> pi0 ee) < 2.8 x
10^(-10) at 90% CL.Comment: 4 pages, three figure
- …