Dynamics of the planktonic food web in Colgada Lake (Lagunas de Ruidera Natural Park)

In Colgada Lake, one of the 15 lakes belonging to Lagunas de Ruidera Natural Park, the components of the lineal food chain (phytoplankton and metazooplankton) and the microbial loop phytoplankton, metazooplankton, ciliates, autotrophic picoplankton, and bacterioplankton) were studied from June 2003 to December 2004 with a monthly sampling frequency. This lake has monomictic and mesotrophic characteristics and a mean depth of 8 m. Sixty-two species of the phytoplankton community, 27 species of metazooplankton community, and 12 species of ciliates were identified. Phytoplankton and metazooplankton integrated biomass followed seasonal patterns with higher values in summer, up to 105 mg WW/m2 and 2 × 104 mg WW/m2, respectively. Autotrophic picoplankton did not exceed 500 mg WW/m2. Microbial loop components did not show seasonality, and its biomass concentration fluctuated between 500-2500 mg WW/m2 for ciliates and 100- 2000 mg WW/m2 for bacterioplankton. Centric Bacillariophyceae, Cryptophyceae, and Dinophyceae represented most of the phytoplanktonic biomass throughout the studied period, although an increase of cyanobacteria was also observed. Cladocerans and calanoid copepods dominated during summer stratification, rotifers and cyclopoid copepods were present during the winter mixing period. The microbial loop biomass in relation to total planktonic biomass was higher in winter and spring 2004. Interannual changes in the presence of planktonic groups were observed in the different periods of the year: i) the algal composition included larger and non-edible species (Cyanobacteria, Dinophyceae), ii) a reduction in the clearance function by cladocerans was produced, which were substituted by a cyclopoid predator that iii) can feed on rotifers and ciliates, favouring in this way bacterioplankton and autotrophic pico-nanoplankton. This interannual variation could be related to the ongoing eutrophication process in the Lake. All of this may change the way the lake looks: if spring primary production is not strongly controlled by herbivory, this could threaten the annual recruitment of submerged macrophytes that significantly contribute to improve the water quality of the lake.En la laguna Colgada, una de las 15 lagunas que componen el Parque Natural de Las lagunas de Ruidera, se han estudiado los componentes de la red trófica planctónica (fitoplancton, metazooplancton, ciliados, picoplancton autotrófico -PPA- y bacterioplancton), desde junio de 2003 a diciembre de 2004, con una frecuencia de muestreo mensual. Esta laguna posee un carácter monomíctico y mesotrófico y una profundidad media de 8 m. Se identificaron 62 especies de la comunidad fitoplanctónica, 27 especies de la comunidad de metazooplancton y 12 especies de ciliados. La biomasa integrada de fitoplancton y metazooplancton mostró un patrón estacional con valores más elevados en verano, hasta 105 mg PF/m2 y 2 × 104 mg PF/m2, respectivamente. El PPA no superó los 500 mg PF/m2. Bacterias y ciliados no presentaron estacionalidad, y su concentración fluctuó entre 500-2500 mg PF/m2 en el caso de los ciliados y 100-2000 mg PF/m2 en el bacterioplancton. Poblaciones de Bacillariophyceae de tipo centrales, Cryptophyceae y Dinophyceae constituyeron la mayor parte de la biomasa fitoplanctónica durante todo el periodo de estudio, aunque se observó un incremento de cianobacterias. Cladóceros y copépodos calanoides fueron dominantes durante la estratificación estival, rotíferos y copépodos ciclopoides estaban presentes en la mezcla invernal. La biomasa relativa del bucle microbiano frente al total de biomasa planctónica fue superior en inverno y primavera de 2004. Se han observado cambios interanuales en la presencia de los grupos planctónicos de los diferentes periodos del año: i) la composición algal incluye especies más grandes y menos comestibles (Cyanobacteria, Dinophyceae), ii) se produce una reducción de la función de aclarado de los cladóceros que son sustituidos por un depredador ciclopoide que iii) se pue de alimentar de rotíferos y ciliados, favoreciendo así a las bacterias y el pico-nanoplancton autotrófico. Esta variación interanual podría estar relacionada con el proceso de eutrofización que está sufriendo la laguna. Todo ello puede llegar a cambiar el aspecto de la laguna: si la producción primaria en primavera no está fuertemente controlada por la herbivoría, se puede impedir el reclutamiento anual de los macrófitos sumergidos que contribuyen significativamente a mejorar la calidad de la aguas de esta laguna

Variabilidad espacial y temporal en la comunidad de cladóceros de la Ciénaga de Paredes (Santander, Colombia) a lo largo de un ciclo anual

En la Ciénaga de Paredes (73 · 45'-73· 49'W y 7·26'-7·29'N), ubicada en el Departamento de Santander (Colombia), se determinó la composición, la variación espacial y temporal de la estructura de la comunidad de cladóceros, con base en arrastres verticales con malla de 68 μm, en ocho estaciones de muestreo en un ciclo anual (febrero de 1998 a enero de 1999).Para evaluar la estructura, se utilizaron los números de Hill (N0, N1 y N2) y la equidad. El soporte del muestreo fue calculado con los estimadores Chao 1 y 2. La existencia de diferencias significativas de los números de Hill, la equidad, la densidad numérica, la columna de agua, el pH, el OD, y la temperatura, entre campañas y entre estaciones, se realizó a través de un ANDEVA. Las especies y morfoespecies encontradas (31) poseen distribución tropical, subtropical y cosmopolita; pertenecen generalmente a cuerpos de agua temporales, llanuras de inundación o ciénagas. Las mayores abundancias fueron registradas para Moina minuta, Moina cf. micrura, Diaphanosoma brevireme y Ceriodaphnia cornuta, las cuales representaron el 81.9 % del total de individuos colectados. Los resultados obtenidos por los estimadores de riqueza indican que si se aumentara el esfuerzo de muestreo con las técnicas utilizadas, no incrementaría el número de morfoespecies. Con respecto a la variación espacial de la estructura, la estación V presento mayor equidad, riqueza y diversidad, pero menor densidad numérica, esta condición muestra la diferencia de esta estación en comparación con las demás; su tendencia atípica es explicada ya que dicha estación se encuentra cerca del afluente principal de la Ciénaga (Quebrada La Gómez). En la variación temporal, la estructura de la comunidad de cladóceros cambió entre campañas de muestreo ya que la equidad y la riqueza presentaron diferencias significativas, que se evidencian en el cambio de la abundancia relativa de las morfoespecies, mas no en la abundancia de cladóceros. Esto es causado por las fluctuaciones de la precipitación y el alto de la columna de agua.In the Ciénaga de Paredes (73 · 45'-73· 49'W y 7·26'-7·29'N), located in the Department of Santander (Colombia), the composition, and the spatial and temporal variation of the cladoceran community structure was determined with samples taken with a 68 μm vertical-hauled net, at eight sampling stations in an annual cycle (February 1998 to January 1999). To evaluate the structure, Hill numbers (N0, N1 and N2) and evenness were used. The sampling support was calculated with the Chao 1 and 2 estimators. The existence of significant differences for Hill numbers, evenness, numeric density, water column, pH, OD, and temperature among field trips and among stations, was analysed through an ANOVA. The species and morphospecies found (31), had a tropical, subtropical, and cosmopolitan distribution; belonging to temporary water bodies, floodplains or "ciénagas". The highest abundances were registered for Moina minuta, Moina cf. micrura, Diaphanosoma brevireme and Ceriodaphnia cornuta, which represented 81.9 % of the total collected individuals. The results obtained with the richness estimates suggest that if the sampling effort were increased using the same techniques, the morphospecies' number would have not increased. With regard to the structure's spatial variation, the station V showed higher evenness, richness, and diversity, but lower numeric density; this condition shows the difference between this station and the other ones; ical trend is explained by this station being near to the main tributary of the "Ciénaga" ("Quebrada La Gómez").Regarding the temporal variation, the cladocerans' community structure changed between field trips, since the evenness and the richness showed significant differences, reflected by the variation in the relative abundance of the morphospecies but not in the cladocerans' abundance. This was caused by the fluctuations in rainfall and water level

Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components. Dendritic cells (DCs) play a key role in the defense against viral infections, for instance plasmacytoid DCs (pDCs), have the capacity to produce vast amounts of interferon-alpha (IFN-α). In COVID-19 there is a deficit in DC numbers and IFN-α production, which has been associated with disease severity. In this work, we described that in addition to the DC deficiency, several DC activation and homing markers were altered in acute COVID-19 patients, which were associated with multiple inflammatory markers. Remarkably, previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+ myeloid DCs and pDCs seven months after SARS-CoV-2 infection. Moreover, the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients, while no restoration of integrin β7 and indoleamine 2,3-dyoxigenase (IDO) levels were observed. These findings contribute to a better understanding of the immunological sequelae of COVID-19

Consumption of caffeinated beverages and kidney function decline in an elderly Mediterranean population with metabolic syndrome

It remains unclear whether caffeinated beverages could have deleterious renal effects in elderly population with underlying comorbid conditions. We investigated the associations between coffee, tea, or caffeine intake and 1-year changes in glomerular filtration rate (eGFR) in a large Spanish cohort of overweight/obese elderly with metabolic syndrome (MetS). This prospective analysis includes 5851 overweight/obese adults (55-75 years) with MetS from the PREDIMED-Plus study. We assessed coffee, tea, and caffeine consumption from a validated food-frequency questionnaire and creatinine-based eGFR using the Chronic Kidney Disease Epidemiology Collaboration equation. Multivariate-adjusted regression models were applied to test associations between baseline coffee, tea, or caffeine intake and 1-year eGFR changes. Caffeinated coffee (> 2 cups/day) and tea (at least 1 cup/day) drinkers had 0.88 and 0.93 mL/min/1.73 m2 greater eGFR decrease respectively, compared to those with less than 1 cup/day of coffee consumption or non-tea drinkers. Furthermore, caffeinated coffee consumption of > 2 cups/day was associated with 1.19-fold increased risk of rapid eGFR decline > 3 mL/min/1.73 m2 (95% CI 1.01-1.41). Similarly, individuals in the highest (median, 51.2 mg/day) tertile of caffeine intake had a 0.87 mL/min/1.73 m2 greater eGFR decrease. Decaffeinated coffee was not associated with eGFR changes. In conclusion, higher consumption of caffeinated coffee, tea, and caffeine was associated with a greater 1-year eGFR decline in overweight/obese adults with MetS

Integrative development of a short screening questionnaire of highly processed food consumption (sQ-HPF)

Background: Recent lifestyle changes include increased consumption of highly processed foods (HPF), which has been associated with an increased risk of non-communicable diseases (NCDs). However, nutritional information relies on the estimation of HPF consumption from food-frequency questionnaires (FFQ) that are not explicitly developed for this purpose. We aimed to develop a short screening questionnaire of HPF consumption (sQ-HPF) that integrates criteria from the existing food classification systems. Methods: Data from 4400 participants (48.1% female and 51.9% male, 64.9 +/- 4.9 years) of the Spanish PREDIMED-Plus (PREvention with MEDiterranean DIet) trial were used for this analysis. Items from the FFQ were classified according to four main food processing-based classification systems (NOVA, IARC, IFIC and UNC). Participants were classified into tertiles of HPF consumption according to each system. Using binomial logistic regression, food groups associated with agreement in the highest tertile for at least two classification systems were chosen as items for the questionnaire. ROC analysis was used to determine cut-off points for the frequency of consumption of each item, from which a score was calculated. Internal consistency of the questionnaire was assessed through exploratory factor analysis (EFA) and Cronbach's analysis, and agreement with the four classifications was assessed with weighted kappa coefficients. Results: Regression analysis identified 14 food groups (items) associated with high HPF consumption for at least two classification systems. EFA showed that items were representative contributors of a single underlying factor, the HPF dietary pattern (factor loadings around 0.2). We constructed a questionnaire asking about the frequency of consumption of those items. The threshold frequency of consumption was selected using ROC analysis. Comparison of the four classification systems and the sQ-HPF showed a fair to high agreement. Significant changes in lifestyle characteristics were detected across tertiles of the sQ-HPF score. Longitudinal changes in HPF consumption were also detected by the sQ-HPF, concordantly with existing classification systems. Conclusions: We developed a practical tool to measure HPF consumption, the sQ-HPF. This may be a valuable instrument to study its relationship with NCDs

Pro-vegetarian food patterns and cardiometabolic risk in the PREDIMED-Plus study: a cross-sectional baseline analysis

[Purpose]: We explored the cross-sectional association between the adherence to three different provegetarian (PVG) food patterns defined as general (gPVG), healthful (hPVG) and unhealthful (uPVG), and the cardiometabolic risk in adults with metabolic syndrome (MetS) of the PREDIMED-Plus randomized intervention study. [Methods]: We performed a cross-sectional analysis of baseline data from 6439 participants of the PREDIMED-Plus randomized intervention study. The gPVG food pattern was built by positively scoring plant foods (vegetables/fruits/legumes/grains/potatoes/nuts/olive oil) and negatively scoring, animal foods (meat and meat products/animal fats/eggs/fish and seafood/dairy products). The hPVG and uPVG were generated from the gPVG by adding four new food groups (tea and coffee/fruit juices/sugar-sweetened beverages/sweets and desserts), splitting grains and potatoes and scoring them differently. Multivariable-adjusted robust linear regression using MM-type estimator was used to assess the association between PVG food patterns and the standardized Metabolic Syndrome score (MetS z-score), a composed index that has been previously used to ascertain the cardiometabolic risk, adjusting for potential confounders. [Results]: A higher adherence to the gPVG and hPVG was associated with lower cardiometabolic risk in multivariable models. The regression coefficients for 5th vs. 1st quintile were − 0.16 (95% CI: − 0.33 to 0.01) for gPVG (p trend: 0.015), and − 0.23 (95% CI: − 0.41 to − 0.05) for hPVG (p trend: 0.016). In contrast, a higher adherence to the uPVG was associated with higher cardiometabolic risk, 0.21 (95% CI: 0.04 to 0.38) (p trend: 0.019). [Conclusion]: Higher adherence to gPVG and hPVG food patterns was generally associated with lower cardiovascular risk, whereas higher adherence to uPVG was associated to higher cardiovascular risk.This work was supported by the official Spanish Institutions for funding scientific biomedical research, CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN) and Instituto de Salud Carlos III (ISCIII), through the Fondo de Investigación para la Salud (FIS), which is co-funded by the European Regional Development Fund (six coordinated FIS projects leaded by JS-S and JVi, including the following projects: PI13/00673, PI13/00492, PI13/00272, PI13/01123, PI13/00462, PI13/00233, PI13/02184, PI13/00728, PI13/01090, PI13/01056, PI14/01722, PI14/00636, PI14/00618, PI14/00696, PI14/01206, PI14/01919, PI14/00853, PI14/01374, PI14/00972, PI14/00728, PI14/01471, PI16/00473, PI16/00662, PI16/01873, PI16/01094, PI16/00501, PI16/00533, PI16/00381, PI16/00366, PI16/01522, PI16/01120, PI17/00764, PI17/01183, PI17/00855, PI17/01347, PI17/00525, PI17/01827, PI17/00532, PI17/00215, PI17/01441, PI17/00508, PI17/01732, PI17/00926, PI19/00957, PI19/00386, PI19/00309, PI19/01032, PI19/00576, PI19/00017, PI19/01226, PI19/00781, PI19/01560, PI19/01332, PI20/01802, PI20/00138, PI20/01532, PI20/00456, PI20/00339, PI20/00557, PI20/00886, PI20/01158); the Especial Action Project entitled: Implementación y evaluación de una intervención intensiva sobre la actividad física Cohorte PREDIMED-Plus grant to JS-S; the European Research Council (Advanced Research Grant 2014–2019; agreement #340918) granted to MÁM-G.; the Recercaixa (number 2013ACUP00194) grant to JS-S; grants from the Consejería de Salud de la Junta de Andalucía (PI0458/2013, PS0358/2016, PI0137/2018); the PROMETEO/2017/017 grant from the Generalitat Valenciana; the SEMERGEN grant; None of the funding sources took part in the design, collection, analysis, interpretation of the data, or writing the report, or in the decision to submit the manuscript for publication

Mediterranean, DASH, and MIND Dietary Patterns and Cognitive Function: The 2-Year Longitudinal Changes in an Older Spanish Cohort

Background and Aims: Plant-forward dietary patterns have been associated with cardiometabolic health benefits, which, in turn, have been related to cognitive performance with inconsistent findings. The objective of this study was to examine the relationship between baseline adherence to three a priori dietary patterns (Mediterranean, DASH, and MIND diets) with 2-year changes in cognitive performance in older adults with overweight or obesity and high cardiovascular disease risk. Methods: A prospective cohort analysis was conducted within the PREDIMED-Plus trial, involving 6,647 men and women aged 55-75 years with overweight or obesity and metabolic syndrome. Using a validated, semiquantitative 143-item food frequency questionnaire completed at baseline, the dietary pattern adherence scores were calculated. An extensive neuropsychological test battery was administered at baseline and 2-year follow-up. Multivariable-adjusted linear regression models were used to assess associations between 2-year changes in cognitive function z-scores across tertiles of baseline adherence to the a priori dietary patterns. Results: Adherence to the Mediterranean diet at baseline was associated with 2-year changes in the general cognitive screening Mini-Mental State Examination (MMSE, β: 0.070; 95% CI: 0.014, 0.175, P-trend = 0.011), and two executive function-related assessments: the Trail Making Tests Part A (TMT-A, β: −0.054; 95% CI: −0.110, − 0.002, P-trend = 0.047) and Part B (TMT-B, β: −0.079; 95% CI: −0.134, −0.024, P-trend = 0.004). Adherence to the MIND diet was associated with the backward recall Digit Span Test assessment of working memory (DST-B, β: 0.058; 95% CI: 0.002, 0.114, P-trend = 0.045). However, higher adherence to the DASH dietary pattern was not associated with better cognitive function over a period of 2 years. Conclusion: In older Spanish individuals with overweight or obesity and at high cardiovascular disease risk, higher baseline adherence to the Mediterranean dietary pattern may be associated with better cognitive performance than lower adherence over a period of 2 years

Impact of COVID-19 pandemic on the PREDIMED-Plus randomized clinical trial: Effects on the interventions, participants follow-up, and adiposity

Background: The COVID-19 pandemic has affected the implementation of most ongoing clinical trials worldwide including the PREDIMED-Plus study. The PREDIMED-Plus is an ongoing, multicenter, controlled intervention trial, aimed at weight-loss and cardiovascular disease prevention, in which participants were randomized (1:1 ratio) to an intervention group (energy-reduced Mediterranean diet, promotion of physical activity, and behavioral support) or to a control group (Mediterranean diet with usual care advice). When the pandemic began, the trial was in the midst of the planned intervention. The objective of this report was to examine the effects of the pandemic on the delivery of the intervention and to describe the strategies established to mitigate the possible adverse effects of the pandemic lockdown on data collection and adiposity. Methods: We assessed the integrity of the PREDIMED-Plus trial during 5 identified periods of the COVID-19 pandemic determined according to restrictions dictated by the Spanish government authorities. A standardized questionnaire was delivered to each of the 23 PREDIMED-Plus recruiting centers to collected data regarding the trial integrity. The effect of the restrictions on intervention components (diet, physical activity) was evaluated with data obtained in the three identified lockdown phases: pre lockdown, lockdown proper, and post lockdown. Results: During the lockdown (March/2020-June/2021), 4,612 participants (48% women, mean age 65y) attended pre-specified yearly follow-up visits to receive lifestyle recommendations and obtain adiposity measures. The overall mean (SD) of the proportions reported by each center showed that 40.4% (25.4) participants had in-person visits, 39.8% (18.2) participants were contacted by telephone and 35% (26.3) by electronic means. Participants' follow-up and data collection rates increased across lockdown periods (from ≈10% at onset to ≈80% at the end). Compared to pre-lockdown, waist circumference increased during (0.75 cm [95% CI: 0.60-0.91]) and after (0.72 cm [95% CI: 0.56-0.89]) lockdown. Body weight did not change during lockdown (0.01 kg [95% CI: -0.10 to 0.13) and decreased after lockdown (-0.17 kg [95% CI: -0.30 to -0.04]). Conclusion: Mitigating strategies to enforce the intervention and patient's follow-up during lockdown have been successful in preserving the integrity of the trial and ensuring its continuation, with minor effects on adiposity. Clinical trial registration: https://doi.org/10.1186/ISRCTN89898870, identifier ISRCTN89898870. Keywords: COVID-19; Mediterraean diet; PREDIMED-Plus; clinical trial; lockdown; weight-loss. Copyright © 2023 Paz-Graniel, Fitó, Ros, Buil-Cosiales, Corella, Babio, Martínez, Alonso-Gómez, Wärnberg, Vioque, Romaguera, López-Miranda, Estruch, Tinahones, Lapetra, Serra-Majem, Bueno-Cavanillas, Tur, Martín-Sánchez, Pintó, Gaforio, Matía-Martín, Vidal, Vázquez, Daimiel, García-Gavilán, Toledo, Nishi, Sorlí, Castañer, García-Ríos, García de la Hera, Barón-López, Ruiz-Canela, Morey, Casas, Garrido-Garrido, Tojal-Sierra, Fernández-García, Vázquez-Ruiz, Fernández-Carrión, Goday, Peña-Orihuela, Compañ-Gabucio, Schröder, Martínez-Gonzalez and Salas-Salvadó. Conflict of interest statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants

© 2015 Castillo-Lizardo et al. Background: The phytohormone indole-3-acetic acid (IAA) is widely distributed among plant-associated bacteria. Certain strains of the Pseudomonas syringae complex can further metabolize IAA into a less biologically active amino acid conjugate, 3-indole-acetyl-ε-L-lysine, through the action of the iaaL gene. In P. syringae and Pseudomonas savastanoi strains, the iaaL gene is found in synteny with an upstream gene, here called matE, encoding a putative MATE family transporter. In P. syringae pv. tomato (Pto) DC3000, a pathogen of tomato and Arabidopsis plants, the HrpL sigma factor controls the expression of a suite of virulence-associated genes via binding to hrp box promoters, including that of the iaaL gene. However, the significance of HrpL activation of the iaaL gene in the virulence of Pto DC3000 is still unclear. Results: A conserved hrp box motif is found upstream of the iaaL gene in the genomes of P. syringae strains. However, although the promoter region of matE is only conserved in genomospecies 3 of this bacterial group, we showed that this gene also belongs to the Pto DC3000 HrpL regulon. We also demonstrated that the iaaL gene is transcribed both independently and as part of an operon with matE in this pathogen. Deletion of either the iaaL or the matE gene resulted in reduced fitness and virulence of Pto DC3000 in tomato plants. In addition, we used multicolor fluorescence imaging to visualize the responses of tomato plants to wild-type Pto DC3000 and to its ΔmatE and ΔiaaL mutants. Activation of secondary metabolism prior to the development of visual symptoms was observed in tomato leaves after bacterial challenges with all strains. However, the observed changes were strongest in plants challenged by the wild-type strain, indicating lower activation of secondary metabolism in plants infected with the ΔmatE or ΔiaaL mutants. Conclusions: Our results provide new evidence for the roles of non-type III effector genes belonging to the Pto DC3000 HrpL regulon in virulence.This research was supported by the Spanish Plan Nacional I+D+i grants AGL2011-30343-CO2-01, AGL2014-53242-C2-1-R and BIO2007-67874-C02-02 as well as by grants ref. P08-CVI-03475 and P12-AGR-0370 from the Junta de Andalucía (Spain).Peer Reviewe