Draft Genome Sequences of Five Enterococcus Species Isolated from the Gut of Patients with Suspected Clostridium difficile Infection

Indexación: Scopus.We present draft genome sequences of five Enterococcus species from patients suspected of Clostridium difficile infection. Genome completeness was confirmed by presence of bacterial orthologs (97%). Gene searches using Hidden-Markov models revealed that the isolates harbor between seven and 11 genes involved in antibiotic resistance to tetracyclines, beta-lactams, and vancomycin.http://genomea.asm.org/content/5/20/e00379-17.ful

¿voluntad de vivir o voluntad de morir? el suicidio en schopenhauer y mainländer

Revista de Filosofía (Universidad de Chile: Santiago, Chile) 63 (2007): 117-126

The trimer interface in the quaternary structure of the bifunctional prokaryotic FAD synthetase from Corynebacterium ammoniagenes

Bifunctional FAD synthetases (FADSs) fold in two independent modules; The C-terminal riboflavin kinase (RFK) catalyzes the RFK activity, while the N-terminal FMN-adenylyltransferase (FMNAT) exhibits the FMNAT activity. The search for macromolecular interfaces in the Corynebacterium ammoniagenes FADS (CaFADS) crystal structure predicts a dimer of trimers organization. Within each trimer, a head-to-tail arrangement causes the RFK and FMNAT catalytic sites of the two neighboring protomers to approach, in agreement with active site residues of one module influencing the activity at the other. We analyze the relevance of the CaFADS head-to-tail macromolecular interfaces to stabilization of assemblies, catalysis and ligand binding. With this aim, we evaluate the effect of point mutations in loop L1c-FlapI, loop L6c, and helix a1c of the RFK module (positions K202, E203, F206, D298, V300, E301 and L304), regions at the macromolecular interface between two protomers within the trimer. Although none of the studied residues is critical in the formation and dissociation of assemblies, residues at L1c-FlapI and helix a1c particularly modulate quaternary architecture, as well as ligand binding and kinetic parameters involved with RFK and FMNAT activities. These data support the influence of transient oligomeric structures on substrate accommodation and catalysis at both CaFADS active sites

Influence of different dietary fats on triacylglycerol deposition in rat adipose tissue

It has been demonstrated that triacylglycerol (TAG) mobilization from adipose tissue is selective and depends on fatty acid (FA) chain length, unsaturation and positional isomerism. The present study was performed to determine the influence of dietary fat on the composition of TAG stored in rat perirenal and subcutaneous adipose tissues. These results may provide information on the susceptibility of stored TAG to hydrolysis and further mobilization, and may help to establish an interrelationship between dietary composition and the FA efflux from adipose tissue. TAG molecular species and FA composition were determined by HPLC and GLC respectively. No significant differences were found in either FA or TAG composition between perirenal and subcutaneous adipose depots. The major FA in the dietary fats were present in the adipose tissues of the animals; in most cases, in similar proportions. However, differences were found between dietary and adipose tissue content of minor FA, which suggests that dietary FA composition is altered between ingestion and deposition in adipose tissue. The TAG molecular species of rat adipose tissue were enriched with the FA characteristic of each dietary fat. Dietary sunflower oil was responsible for enrichment with the most polar TAG. This finding may suggest easier mobilization of stored TAG. In conclusion, the process of fatty acid and TAG deposition in rat adipose tissue is selective, and depends on the composition of the diet.Supported by grants (OLI96-2126 and ALI99-0863) from the Comisión Interministerial de Ciencia y Tecnología (CICYT) and the Government of the País Vasco (PI 96/22) and by a felIowship from this Government

Evaluación de riesgo de incendio urbano en el centro histórico de la ciudad de Guimarães

The main objective of this work was to evaluate the urban fire risk of the Historic Centre of Guimarães, Portugal, which was declared by UNESCO as a World Heritage Site in 2001. From the study of the singular urban, architectural and constructive characteristics of Historic Centre of Guimarães, the present research focused on the analysis of a broad set of information collected in situ and on its subsequent use to apply a simplified urban fire risk assessment methodology. A GIS tool (Geographic Information System) was used to process and plot the results, allowing to obtain vulnerability maps from which it is possible to assess the global vulnerability of the Historical Centre of Guimarães, as well as to identify the areas where, from the evaluation of four global factors related with the fire ignition, propagation, evacuation and combat, urban fire risk is potentially higher.El principal objetivo de este trabajo fue evaluar el riesgo de incendio urbano en el Centro Histórico de Guimaraes, Portugal, declarado Patrimonio Cultural de la Humanidad en 2001 por la UNESCO. A partir del estudio de las caráctertísticas urbanas, arquitectónicas y constructivas del Centro Histórico de Guimarães, la presente investigación se centró en el análisis de un amplio conjunto de información recogida in situ y en su posterior utilización para la aplicación de una metodología simplificada de evaluación de riesgo de incendio urbano. Los datos obtenidos fueron procesados con la herramienta GIS (Geographic Information System), preparándose planos donde se muestra el nivel de vulnerabilidad del Centro Histórico de Guimarães, además de detectar las zonas de mayor riesgo en función de los cuatro factores globales de riesgo asociado al inicio, propagación, evacuación y combate al incendio

Lactante con mal progreso pondo-estatural y letargia

Paciente de 4 meses de edad, sexo masculino, que consulta por presentar retardo pondoestatural y mala conducta alimentaria. Antecedentes: - Recién nacido de término, peso: 3400 gr. - Embarazo controlado, infección urinaria tercer trimestre. - Parto eutósico, presentación cefálica, 2º gesta. - Serologías negativas para: VDRL, Chagas, Toxoplasmosis e HIV. - Caída del cordón umbilical al mes de vida. - Pesquisa neonatal para tirotrofina (TSH), 17 hidroxiprogesterona (17 OHP4),  Tripsina, Fenilalanina, normal. - Permaneció internado en neonatología por sepsis a Klebsiella, requirió ARM   por 4 días y 10 días con halo de oxígeno. - Presentó colestasis a los 10 días de vida, sin hipocolia,  fue dado de alta con ácido ursodesoxicólico. - Madre de 27 años, sana, talla 161 cm, menarca 14 años de edad. - Padre  de 28 años, sano, talla 175 cm. - No consanguíneos.- Hermana de 3 años sana.Fil: Dujovne, Dras N.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Canteros, V.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Aguiar, M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Warman, M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Baquedano, S.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Herzovich, V.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Belgorosky, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentin

How Strong Do Global Commodity Prices Influence Domestic Food Prices in Developing Countries? A Global Price Transmission and Vulnerability Mapping Analysis

This paper analyzes the transmission from global commodity to domestic food prices for a large set of countries. First, a theoretical model is developed to explain price transmission for different trade regimes. Drawing from the competitive storage model under rational expectations, it is shown that domestic prices can respond instantaneously to global prices even if no trade takes place but future trade is expected. Using a global database on food prices, we construct national and international grain price indices. With an autoregressive distributed lag model, we empirically detect countries in which food prices are influenced by global commodity prices, including futures prices. Mapping transmission elasticities with the size of the population below the poverty line which spends typically a large share of its income on food, we are able to estimate the size of vulnerable population. Our empirical analysis reveals that 90 percent of the global poor (income below 1.25$/day) live in countries where domestic food prices respond to international prices - but the extent of transmission varies substantially. For 360 million poor people, international prices transmit to their country at rates of 30 percent or higher within three months

Evidence of fatal skeletal injuries on Malapa Hominins 1 and 2

Malapa is one of the richest early hominin sites in Africa and the discovery site of the hominin species, Australopithecus sediba. The holotype and paratype (Malapa Hominin 1 and 2, or MH1 and MH2, respectively) skeletons are among the most complete in the early hominin record. Dating to approximately two million years BP, MH1 and MH2 are hypothesized to have fallen into a natural pit trap. All fractures evident on MH1 and MH2 skeletons were evaluated and separated based on wet and dry bone fracture morphology/characteristics. Most observed fractures are post-depositional, but those in the right upper limb of the adult hominin strongly indicate active resistance to an impact, while those in the juvenile hominin mandible are consistent with a blow to the face. The presence of skeletal trauma independently supports the falling hypothesis and supplies the first evidence for the manner of death of an australopith in the fossil record that is not attributed to predation or natural death