Image Analysis on Bacteria Time-Lapse Movies

This study has been developed a methodology for identifying accurately the boundaries of individual bacterial cells and tracking them from frame to frame so as to construct the cells’ genealogy (bacterial cell segmentation and lineage tree construction) even in large-size microbial communities where there is great difficulty in identifying the individual cell boundaries

Fusing face and body display for Bi-modal emotion recognition: Single frame analysis and multi-frame post integration

This paper presents an approach to automatic visual emotion recognition from two modalities: expressive face and body gesture. Pace and body movements are captured simultaneously using two separate cameras. For each face and body image sequence single "expressive" frames are selected manually for analysis and recognition of emotions. Firstly, individual classifiers are trained from individual modalities for mono-modal emotion recognition. Secondly, we fuse facial expression and affective body gesture information at the feature and at the decision-level. In the experiments performed, the emotion classification using the two modalities achieved a better recognition accuracy outperforming the classification using the individual facial modality. We further extend the affect analysis into a whole image sequence by a multi-frame post integration approach over the single frame recognition results. In our experiments, the post integration based on the fusion of face and body has shown to be more accurate than the post integration based on the facial modality only. © Springer-Verlag Berlin Heidelberg 2005

Markedly Different Pathogenicity of Four Immunoglobulin G Isotype-Switch Variants of an Antierythrocyte Autoantibody Is Based on Their Capacity to Interact in Vivo with the Low-Affinity Fcγ Receptor III

Using three different Fcγ receptor (FcγR)-deficient mouse strains, we examined the induction of autoimmune hemolytic anemia by each of the four immunoglobulin (Ig)G isotype-switch variants of a 4C8 IgM antierythrocyte autoantibody and its relation to the contributions of the two FcγR, FcγRI, and FcγRIII, operative in the phagocytosis of opsonized particles. We found that the four IgG isotypes of this antibody displayed striking differences in pathogenicity, which were related to their respective capacity to interact in vivo with the two phagocytic FcγRs, defined as follows: IgG2a > IgG2b > IgG3/IgG1 for FcγRI, and IgG2a > IgG1 > IgG2b > IgG3 for FcγRIII. Accordingly, the IgG2a autoantibody exhibited the highest pathogenicity, ∼20–100-fold more potent than its IgG1 and IgG2b variants, respectively, while the IgG3 variant, which displays little interaction with these FcγRs, was not pathogenic at all. An unexpected critical role of the low-affinity FcγRIII was revealed by the use of two different IgG2a anti–red blood cell autoantibodies, which displayed a striking preferential utilization of FcγRIII, compared with the high-affinity FcγRI. This demonstration of the respective roles in vivo of four different IgG isotypes, and of two phagocytic FcγRs, in autoimmune hemolytic anemia highlights the major importance of the regulation of IgG isotype responses in autoantibody-mediated pathology and humoral immunity

An early history of T cell-mediated cytotoxicity.

After 60 years of intense fundamental research into T cell-mediated cytotoxicity, we have gained a detailed knowledge of the cells involved, specific recognition mechanisms and post-recognition perforin-granzyme-based and FAS-based molecular mechanisms. What could not be anticipated at the outset was how discovery of the mechanisms regulating the activation and function of cytotoxic T cells would lead to new developments in cancer immunotherapy. Given the profound recent interest in therapeutic manipulation of cytotoxic T cell responses, it is an opportune time to look back on the early history of the field. This Timeline describes how the early findings occurred and eventually led to current therapeutic applications