The effect of carob (Ceratonia siliqua) bean extract on male New Zealand White rabbit semen

[EN] The carob tree (Ceratonia siliqua) grows naturally in the Mediterranean region. The empiric use of carob cures for their aphrodisiac properties is very common in Turkey. Thus, the experiment was conducted to determine the effects of carob bean extracts on some reproductive parameters in male New Zealand White rabbits. During the adaptation period (stage 1), 6-8 mo old rabbits were trained in semen collection for 30 d. At the beginning of the treatment period (stage 2), rabbits were assigned randomly to 2 groups of 8 animals each. For a period of 49 d (1 spermatogenesis duration), one group was treated with a daily oral dose (10 mL) of carob extract and the other group received the corresponding volume of tap water. Semen was collected weekly. Semen samples taken at week 1 and 7 were analysed separately. At the beginning of stage 2, no differences were observed in the volume and pH of the ejaculate, sperm concentration, percentage of motility, percentage of live spermatozoa, percentage of sperm plasma membrane integrity, plasma concentration of testosterone, and seminal plasma protein levels between the control and carob extract treated animals. Similarly, at the end of stage 2, there were no differences in the volume and pH of the ejaculate, motility percentage, the percentage of live spermatozoa, percentage of sperm plasma membrane integrity, and the seminal plasma protein levels between the control and the carob extract treated animals. However, sperm concentration (P<0.05), plasma concentration of testosterone (P<0.05), and percentage of change in spermatozoa concentration (P<0.02) between groups were affected at the end of stage 2. The data suggested that the use of carob cures prepared by boiling carob fruit could have beneficial influences on sperm concentration in rabbits.Ata, A.; Yildiz-Gulay, O.; Güngör, S.; Balic, A.; Gulay, M. (2018). The effect of carob (Ceratonia siliqua) bean extract on male New Zealand White rabbit semen. World Rabbit Science. 26(3):209-215. doi:10.4995/wrs.2018.10154SWORD209215263Abd El-Ghany M.A. 2007. The relation of antioxidants and sodium nitrite on the oxidation reduction system and reproductive ability of male rats. Egypt J. Nutr., 2: 33-64.Agnes V.F., Akbarsha M.A. 2003. Spermatotoxic effect of aflatoxin B1 in the albino Mouse. Food. Chem. Tox., 41: 119-130. https://doi.org/10.1016/S0278-6915(02)00171-0Amico F.P., Source E.G. 1997. Medical plants and phytotheraphy in Mussomeli area (Caltenisseta, Scily, Italy). Fitoterapia, 68: 143-159.Ata A., Hatipoglu F.S., Yildiz-Gulay O., Gulay M.S. 2007. Protective role of ascorbic acid on subacute sperm toxicity in male New Zealand White Rabbits treated with endosulfan. Drug Chem. Toxicol., 30: 181-195. https://doi.org/10.1080/01480540701374896Ayaz F.A., Torun H., Ayaz S., Correia P.J., Alaiz M., Sanz C., Gruz J., Strand M. 2007. Determination of chemical composition of Anatolian carob pod (Ceratonia siliqua L.), Sugars, amino and organic acids, minerals and phenolic compounds. J. Food Quality, 30: 1040-1055. https://doi.org/10.1111/j.1745-4557.2007.00176.xFeng P., Li TL., Guan Z.X., Franklin R.B., Costello L.C. 2002. Direct effect of zinc on mitochondrial apoptogenesis in prostate cells. Prostate, 52: 311-318. https://doi.org/10.1002/pros.10128Hala M.A.W. 2011. Protective effect of Nigella sativa, linseed and celery oil against testicular toxicity induced by sodium valproate in male rats. J. Am. Sci., 7: 687-693.Inouce M., Suzuki R., Koide T., Sakaguchi N., Ogihera Y., Yabu Y. 1994. Antioxidant, gallic acid, induces apaptosis in HL- 60RG cells. Biochem. Res. Commun., 204: 898-904. https://doi.org/10.1006/bbrc.1994.2544Karkacier M., Artik N. 1995. Determination of physical properties, chemical composition and extraction conditions of carob bean (Ceratonia siliqua L.). Gida, 20: 131-136.Katsiya G.V., Todua T.N., Gorlushkin V.M., Chirkov A.M., Goncharov N.P. 1989. Effect of immobilization stress on the gonadotropic function of the hypophysis in male hamadryas baboons (Papio hamadryas). Biol. Medit., 107: 231-234.Marakis S. 1996. Carob bean in food and feed: current status and future potentials: a critical appraisal. J. Food Sci. Technol., 33: 365-383.Merzouki A., Ed-Derfoufi F., El Allau A., Molero-Mesas J. 1997. Wild medicinal plants used by local Bouhmed population (Morocco). Fitoterapia, 68: 444-460.Rickard I.J., Holopainen J., Helama S., Helle S., Russell A.F., Lummaa V. 2010. Food availability at birth limited reproductive success in historical humans. Ecology, 91: 3515-3525. https://doi.org/10.1890/10-0019.1Robbins W.A., Xun L., FitzGerald L.Z., Esguerra S., Henning S.M., Carpenter C.L. 2012. Walnuts Improve Semen Quality in Men Consuming a Western-Style Diet: Randomized Control Dietary Intervention Trial. Biol. Reprod., 87: 101-108. https://doi.org/10.1095/biolreprod.112.101634Osaretin A.T., Gabriel A.A. 2008. Effect of zinc deficiency on memory, oxidative stress and blood chemistry in rats. Adv. Med. Dent. Sci., 2: 74-82.Saracoglu A.I. 2011. Şifa Kitabı, 1st ed. Haygrup Publishers, Istanbul, Turkey.Takeda A., Tamano H., Tochigi M. 2005. Zinc homeostasis in the hypocampus of zinc deficient young adult rats. J. Neurochem. Int., 46: 221-225. https://doi.org/10.1016/j.neuint.2004.10.003Tokeshi I., Yoshimoto T., Muto N., Nakamura S., Ashizawa K., Nakada T., Tatemoto H. 2007. Antihyaluronidase action of ellagic acid effectively prevents polyspermy as a result of suppression of the acrosome reaction induced by sperm-zona interaction during in vitro fertilization of porcine oocytes. J. Reprod. Develop., 53: 755-764. https://doi.org/10.1262/jrd.18173Turk G., Sonmez M., Aydin M., Yuce A., Gur S., Yuksel M., Aksu E.H., Aksoy H. 2008. Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clin. Nutr., 27: 289-296. https://doi.org/10.1016/j.clnu.2007.12.006Würsch P. 1979. Influence of tannin-rich carob pod fiber on the cholesterol metabolism in the rat. J. Nutr., 109: 685-692. https://doi.org/10.1093/jn/109.4.685Zago M.P., Oteiza P.I. 2001. The antioxidant properties of zinc: interactions with iron and antioxidants. Free Rad. Biol. Med., 31: 266-274. https://doi.org/10.1016/S0891-5849(01)00583-

Life-history trait of the Mediterranean keystone species Patella rustica: growth and microbial bioerosion

The age and shell growth patterns in populations of Patella rustica of the Adriatic Sea were determined by analyzing the inner growth lines visible in shell sections. Marginal increment analysis showed annual periodicity with annual growth line being deposited in May. The growth analysis of 120 individual shells showed that 90.8 % of collected individuals were less than 4 years of age and only two individuals (1.6 %) were older than 6 years. Population structure was described and the generalized von Bertalanffy growth parameters were calculated: asymptotic length (L∞) was 38.22 mm and the growth constant (K) was 0.30 year-1. Growth performance index value of P. rustica (Ø’) was 2.64 and is among the lowest ranges reported for limpet species. Patella rustica shells were degraded to different degrees by microbial bioerosion. Microboring organisms identified were pseudofilamentous and filamentous cyanobacteria Hormathonema paulocellulare, Hyella caespitosa, Mastigocoleus testarum and Leptolyngbya sp. The overall intensity of infestation was relatively low, but increased in severity with shell length. The damage was most often restricted to the oldest parts of the shell, i.e. apex of the shell, posing difficulties in determining the exact position of the first growth line. The present study is first to introduce the use of inner growth lines in Patella rustica shell sections as a reliable method for age determination and it provides the first insight into the growth patterns of this keystone species while taking the interference of microbial shell bioerosion in consideration

Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation

Enamel is secreted by ameloblasts derived from tooth epithelial stem cells (SCs). Humans cannot repair or regenerate enamel, due to early loss of tooth epithelial SCs. Contrarily in the mouse incisors, epithelial SCs are maintained throughout life and endlessly generate ameloblasts, and thus enamel. Here we isolated Sox2-GFP+ tooth epithelial SCs which generated highly cellular spheres following a novel in vitro strategy. This system enabled analysis of SC regulation by various signaling molecules, and supported the stimulatory and inhibitory roles of Shh and Bmp, respectively; providing better insight into the heterogeneity of the SCs. Further, we generated a novel mouse reporter, Enamelin-tdTomato for identification of ameloblasts in live tissues and cells, and used it to demonstrate presence of ameloblasts in the new 3D co-culture system of dental SCs. Collectively, our results provide means of generating 3D tooth epithelium from adult SCs which can be utilized toward future generation of enamel.Peer reviewe

Efficient all-optical switching using slow light within a hollow fiber

We demonstrate a fiber-optical switch that is activated at tiny energies corresponding to few hundred optical photons per pulse. This is achieved by simultaneously confining both photons and a small laser-cooled ensemble of atoms inside the microscopic hollow core of a single-mode photonic-crystal fiber and using quantum optical techniques for generating slow light propagation and large nonlinear interaction between light beams

Switching of light with light using cold atoms inside a hollow optical fiber

We demonstrate a fiber-optical switch that operates with a few hundred photons per switching pulse. The light-light interaction is mediated by laser-cooled atoms. The required strong interaction between atoms and light is achieved by simultaneously confining photons and atoms inside the microscopic hollow core of a single-mode photonic-crystal fiber

Exercise-based interventions to enhance long-term sustainability of physical activity in older adults: a systematic review and meta-analysis of randomized clinical trials

Older adults; Physical activity; AdherenceAdultos mayores; Actividad física; AdherenciaAdults majors; Activitat física; AdherènciaExercise is a form of physical activity (PA). PA is an important marker of health and quality of life in older adults. The purpose of this study was to conduct a systematic review of the literature to assess the effect of exercise-based interventions on an at least six-month follow up PA measure, and to describe the specific strategies implemented during the intervention to strengthen the sustainability of PA in community-dwelling 65+ year-old adults. We registered and conducted a systematic review and meta-analysis (PROSPERO: CRD42017070892) of randomized clinical trials (RCT). We searched three electronic databases during January 2018 to identify RCT assessing any type of exercise-based intervention. Studies had to report a pre-, post-, and at least 6-month post-intervention follow-up. To be included, at least one PA outcome had to be assessed. The effect of exercise-based interventions was assessed compared to active (e.g., a low-intensity type of exercise, such as stretching or toning activities) and non-active (e.g., usual care) control interventions at several time points. Secondary analyses were conducted, restricted to studies that reported specific strategies to enhance the sustainability of PA. The intervention effect was measured on self-reported and objective measures of time spent in PA, by means of standardized mean differences. Standardized mean differences of PA level were pooled. Pooled estimates of effect were computed with the DerSimonian–Laird method, applying a random effects model. The risk of bias was also assessed. We included 12 studies, comparing 18 exercise intervention groups to four active and nine non-active control groups. Nine studies reported specific strategies to enhance the long-term sustainability of PA. The strategies were mostly related to the self-efficacy, self-control, and behavior capability principles based on the social cognitive theory. Exercise interventions compared to active control showed inconclusive and heterogeneous results. When compared to non-active control, exercise interventions improved PA time at the six-months follow up (standardized mean difference (SMD) 0.30; 95%CI 0.15 to 0.44; four studies; 724 participants; I2 0%), but not at the one- or two-years follow-ups. No data were available on the mid- and long-term effect of adding strategies to enhance the sustainability of PA. Exercise interventions have small clinical benefits on PA levels in community-dwelling older adults, with a decline in the observed improvement after six months of the intervention cessation.The present study was funded by United States Department of Health & Human Services National Institutes of Health (NIH), USA, and NIH National Institute on Aging (NIA), USA, (K24 AG057728)

Predicting Neutron Production from Cosmic-ray Muons

Fast neutrons from cosmic-ray muons are an important background to underground low energy experiments. The estimate of such background is often hampered by the difficulty of measuring and calculating neutron production with sufficient accuracy. Indeed substantial disagreement exists between the different analytical calculations performed so far, while data reported by different experiments is not always consistent. We discuss a new unified approach to estimate the neutron yield, the energy spectrum, the multiplicity and the angular distribution from cosmic muons using the Monte Carlo simulation package FLUKA and show that it gives a good description of most of the existing measurements once the appropriate corrections have been applied.Comment: 8 pages, 7 figure

Multimodal Treatment Eliminates Cancer Stem Cells and Leads to Long-Term Survival in Primary Human Pancreatic Cancer Tissue Xenografts.

Copyright: 2013 Hermann et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.PURPOSE: In spite of intense research efforts, pancreatic ductal adenocarcinoma remains one of the most deadly malignancies in the world. We and others have previously identified a subpopulation of pancreatic cancer stem cells within the tumor as a critical therapeutic target and additionally shown that the tumor stroma represents not only a restrictive barrier for successful drug delivery, but also serves as a paracrine niche for cancer stem cells. Therefore, we embarked on a large-scale investigation on the effects of combining chemotherapy, hedgehog pathway inhibition, and mTOR inhibition in a preclinical mouse model of pancreatic cancer. EXPERIMENTAL DESIGN: Prospective and randomized testing in a set of almost 200 subcutaneous and orthotopic implanted whole-tissue primary human tumor xenografts. RESULTS: The combined targeting of highly chemoresistant cancer stem cells as well as their more differentiated progenies, together with abrogation of the tumor microenvironment by targeting the stroma and enhancing tissue penetration of the chemotherapeutic agent translated into significantly prolonged survival in preclinical models of human pancreatic cancer. Most pronounced therapeutic effects were observed in gemcitabine-resistant patient-derived tumors. Intriguingly, the proposed triple therapy approach could be further enhanced by using a PEGylated formulation of gemcitabine, which significantly increased its bioavailability and tissue penetration, resulting in a further improved overall outcome. CONCLUSIONS: This multimodal therapeutic strategy should be further explored in the clinical setting as its success may eventually improve the poor prognosis of patients with pancreatic ductal adenocarcinoma

Measurement-Induced Entanglement for Excitation Stored in Remote Atomic Ensembles

A critical requirement for diverse applications in Quantum Information Science is the capability to disseminate quantum resources over complex quantum networks. For example, the coherent distribution of entangled quantum states together with quantum memory to store these states can enable scalable architectures for quantum computation, communication, and metrology. As a significant step toward such possibilities, here we report observations of entanglement between two atomic ensembles located in distinct apparatuses on different tables. Quantum interference in the detection of a photon emitted by one of the samples projects the otherwise independent ensembles into an entangled state with one joint excitation stored remotely in 10^5 atoms at each site. After a programmable delay, we confirm entanglement by mapping the state of the atoms to optical fields and by measuring mutual coherences and photon statistics for these fields. We thereby determine a quantitative lower bound for the entanglement of the joint state of the ensembles. Our observations provide a new capability for the distribution and storage of entangled quantum states, including for scalable quantum communication networks .Comment: 13 pages, 4 figures Submitted for publication on August 31 200