Alternative splicing in lung cancer

Abstract: Alterations in alternative splicing affect essential biologic processes and are the basis for a number of pathologic conditions, including cancer. In this review we will summarize the evidence supporting the relevance of alternative splicing in lung cancer. An example that illustrates this relevance is the altered balance between Bcl-xL and Bcl-xS, two splice variants of the apoptosis regulator Bcl-x. Splice modifications in cancer-related genes can be associated with modifications either in cis-acting splicing regulatory sequences or in trans-acting splicing factors. In fact, lung tumors show abnormal expression of splicing regulators such as ASF/SF2 or some members of the heterogeneous nuclear ribonucleoprotein family. The potential significance of alternative splicing as a target for lung cancer diagnosis or treatment will also be discussed

An inverse source problem for the heat equation and the enclosure method

An inverse source problem for the heat equation is considered. Extraction formulae for information about the time and location when and where the unknown source of the equation firstly appeared are given from a single lateral boundary measurement. New roles of the plane progressive wave solutions or their complex versions for the backward heat equation are given.Comment: 23page

Automating senior fitness testing through gesture detection with depth sensors

Sedentarism has a negative impact on health, life expectancy and quality of life, especially in older adults. The assessment of functional fitness helps evaluating the effects of ageing and sedentarism, and this assessment is typically done through validated battery tests such as the Senior Fitness Test (SFT). In this paper we present a computer-based system for assisting and automating SFT administration and scoring in the elderly population. Our system assesses lower body strength, agility and dynamic balance, and aerobic endurance making use of a depth sensor for body tracking and multiple gesture detectors for the evaluation of movement execution. The system was developed and trained with optimal data collected in laboratory conditions and its performance was evaluated in a real environment with 22 elderly end-users, and compared to traditional SFT administered by an expert. Results show a high accuracy of our system in identifying movement patterns (>95%) and consistency with the traditional fitness assessment method. Our results suggest that this technology is a viable low cost option to assist in the fitness assessment of elderly that could be deployed for at home use in the context of fitness programs.info:eu-repo/semantics/publishedVersio

A new type of arthropod photoreceptor

A new type of photoreceptor for the phylum Arthropoda, found in the class Collembola (Arthropoda, Hexapoda) is reported. This new light-sensitive structure consists of a pair of interocular vesicles present in the genus Vesicephalus Richards, 1964 and is anatomically related to the cluster of ommatidia. The absence of a lens, the presence of a rabdome in the upper part of the vesicle and the reflection and refraction of light by a hemolymph bubble with incidence to the rhabdomeric structure are the main traits of this new photoreceptor

Energy Efficiency Support through Intra-Layer Cloud Stack Adaptation

Energy consumption is a key concern in cloud computing. The paper reports on a cloud architecture to support energy efficiency at service construction, deployment, and operation. This is achieved through SaaS, PaaS and IaaS intra-layer self-adaptation in isolation. The self-adaptation mechanisms are discussed, as well as their implementation and evaluation. The experimental results show that the overall architecture is capable of adapting to meet the energy goals of applications on a per layer basis

Measured and perceived physical responses in multidimensional fitness training through exergames in older adults

Exergames have been used to increase physical activity levels to produce health benefits in older adults. However, only a small number of studies have quantified the physical activity levels produced by custom-made Exergames and their capacity to elicit recommended levels of exercise. This study investigates the effectiveness of custom-made Exergames, designed for multidimensional fitness training, in eliciting recommended levels of exercise. We rely on both objective (accelerometry) and subjective (perceived exertion) information collected in two different modalities of exercise, consisting of 40- minutes sessions: Exergaming and conventional training (Control). A between-subjects analysis was done involving two groups of active older adults (n=33). Participants in the Control Between condition performed physical activity in conventional group fitness training, while the intervention group used individualized Exergaming as training modality. In addition, a sub-group of the Exergaming participants also performed a conventional training session (Control-Within), which enabled a within-subjects comparison. Results show that participants spent significantly more time in moderate-to-vigorous intensities during Exergaming, interestingly, perceiving significantly lower exertion levels. The between-subjects analysis only presented statistically significant differences for the perceived exertion scale. This study helps to unveil the impact of custom-made Exergames in physical activity levels during training when compared to conventional training for the older adult population.info:eu-repo/semantics/publishedVersio

Radiating and non-radiating sources in elasticity

In this work, we study the inverse source problem of a fixed frequency for the Navier's equation. We investigate that nonradiating external forces. If the support of such a force has a convex or non-convex corner or edge on their boundary, the force must be vanishing there. The vanishing property at corners and edges holds also for sufficiently smooth transmission eigenfunctions in elasticity. The idea originates from the enclosure method: The energy identity and new type exponential solutions for the Navier's equation.Comment: 17 page

Novel and natural knockout lung cancer cell lines for the LKB1/STK11 tumor suppressor gene

Germline mutations of the LKB1 gene are responsible for Peutz-Jeghers syndrome (PJS), an autosomal dominant inherited disorder bestowing an increased risk of cancer. We have recently demonstrated that LKB1 inactivating mutations are not confined to PJS, but also appear in lung adenocarcinomas of sporadic origin, including primary tumors and lung cancer cell lines. To accurately determine the frequency of inactivating LKB1 gene mutations in lung tumors we have sequenced the complete coding region of LKB1 in 21 additional lung cancer cell lines. Here we describe the mutational status of LKB1 gene in 30 lung cancer cell lines from different histopathological types, including 11 lung adenocarcinomas (LADs) and 11 small cell lung cancers (SCLCs). LKB1 gene alterations were present in six (54%) of the LAD cell lines tested but in none of the other histological types. Similar to our previous observations in primary tumors, all point mutations were of the nonsense or frameshift type, leading to an abnormal, truncated protein. Moreover, 2 cell lines (A427 and H2126) harbored large gene deletions that spanned several exons. Hence, we have identified additional lung cancer cell lines carrying inactivating mutations of the LKB1 tumor suppressor gene, further attesting to the significance of this gene in the development of LADs and providing new natural LKB1 knockouts for studies of the biological function of the LKB1 protein

Dielectric confinement in quantum dots of arbitrary shape within the local spin density approximation: Diluted regimes in elongated quantum dots

We propose a simplified and computationally feasible model accounting for the dielectric confinement in arbitrarily shaped many-electron quantum dots, within the local spin density approximation. The model yields quite a good agreement with full configuration interaction calculations including exact dielectric confinement. The model is used to study the influence of the dielectric confinement on the electronic charge distribution of elongated quantum dots in the low density regime