Triple‐negative breast carcinomas are a heterogeneous entity that differs between young and old patients

OBJECTIVE: To compare the frequency and immunohistochemical profiles of triple-negative breast carcinomas in younger and older women. METHODS AND RESULTS: We selected patients diagnosed with triple-negative breast carcinomas. The groups examined were women who were 35 years old or younger between 1997 and 2007 (n = 74) and, for comparison, women who were 60 years old or older (n = 19, consecutive cases). All formalin-fixed and paraffin-embedded tumor samples were reviewed and immunohistochemically stained for ER, PR, HER2, Ki-67 antigen, epidermal growth factor receptor, cytokeratin 5/6, p53, vimentin, CD117, and p63 using tissue microarrays blocks. Triple-negative breast carcinomas corresponded to 34.6% (74/213) of the carcinomas from the younger patients and 16.2% (19/117) of the carcinomas from the older patients (p = 0.002). No significant differences in the frequency of the basal phenotype were observed in the two patient groups based on CK5/6 and/or epidermal growth factor receptor expression (74.3% vs. 68.4%). However, triple-negative breast carcinomas in the older patients presented a higher frequency of CK5/6 expression compared to those of younger patients (42.1% vs. 9.6%; p = 0.005), whereas triplenegative breast carcinomas of younger patients had a higher expression level of epidermal growth factor receptor (71.6% vs. 47.3%). CONCLUSIONS: These results show that there were significant molecular differences between the triple-negative basal-like breast carcinomas that were diagnosed in younger women and those that were diagnosed in older women. These findings may provide a basis for describing the more aggressive phenotype of the triple-negative breast carcinomas observed in younger women

Deriving a practical analytical-probabilistic method to size flood routing reservoirs

In the engineering practice routing reservoir sizing is commonly performed by using the design storm method, although its effectiveness has been debated for a long time. Conversely, continuous simulations and direct statistical analyses of recorded hydrographs are considered more reliable and comprehensive, but are indeed complex or seldom practicable. In this paper a handier tool is provided by the analytical-probabilistic approach to construct probability functions of peak discharges issuing from natural watersheds or routed through on-line and off-line reservoirs. A simplified routing scheme and a rainfall-runoff model based on a few essential hydrological parameters were implemented. To validate the proposed design methodology, on-line and off-line routing reservoirs were firstly sized by means of a conventional design storm method for a test watershed located in northern Italy. Their routing efficiencies were then estimated by both analytical-probabilistic models and benchmarking continuous simulations. Bearing in mind practical design purposes, adopted models evidenced a satisfactory consistency

Hydrological aspects of the Mesoscale Alpine Programme: finding from field experiments and simulations

Proc. International Conference on Alpine Metorology, Zagreb 23-27 Ma

Multivariate statistical analysis of flood variables by copulas: two italian case studies

Multivariate statistics are important to determine the flood hydrograph for the design of hydraulic structures and for the hydraulic risk assessment. In the last decade, the copula approach has been investigated in hydrological practice to assess the design flood hydrograph in terms of flood peak, volume and duration. In this paper, the copula approach is exploited to perform pair analyses of these three random variables for two Italian watersheds, in the Apennine and the Alps respectively. The criterion to separate continuous flow series into independent events is discussed along with its implications on the dependence structure. The goodness-of-fits of the proposed copulas are then assessed by non-parametric tests. Marginal distributions to derive joint distributions are briefly suggested. The possibility of generating flood events according to the proposed model and potential applications to hydraulic structure design and flood management are finally examined

Diffuse radio sources in the cluster of galaxies Abell 548b

We report extensive VLA and ATCA observations of the two diffuse radio sources in the cluster of galaxies Abell 548b, which confirm their classification as relics. The two relics (named A and B) show similar flux density, extent, shape, polarization and spectral index and are located at projected distances of about 430 and 500 kpc from the cluster center, on the same side of the cluster's X-ray peak. On the basis of spectral indices of discrete radio sources embedded within the diffuse features, we have attempted to distinguish emission peaks of the diffuse sources from unrelated sources. We have found that both relics, in particular the B-relic, show possible fine structure, when observed at high resolution. Another diffuse source (named C) is detected close in projection to the cluster center. High-resolution images show that it contains two discrete radio sources and a diffuse component, which might be a candidate for a small relic source. The nature and properties of the diffuse radio sources are discussed. We conclude that they are likely related to the merger activity in the cluster.Comment: Accepted for publication in MNRAS. 11 pages, 7 figures. Some figures are degraded to reduce their size. A version with high resolution images is available at http://www.ira.inaf.it/~lferetti/OUTGOING/papA548b.ps.g

Epstein-Barr virus infection and gastric carcinoma in São Paulo State, Brazil

Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and most people have serological evidence of previous viral infection at adult age. EBV is associated with infectious mononucleosis and human cancers, including some lymphomas and gastric carcinomas. Although EBV was first reported in lymphoepithelioma-like gastric carcinoma, the virus was also found in conventional adenocarcinomas. In the present study, 53 gastric carcinomas diagnosed in São Paulo State, Brazil, were evaluated for EBV infection by non-isotopic in situ hybridization with a biotinylated probe (Biotin-AGACACCGTCCTCACCACCC GGGACTTGTA) directed to the viral transcript EBER-I, which is actively expressed in EBV latently infected cells. EBV infection was found in 6 of 53 (11.32%) gastric carcinomas, mostly from male patients (66.7%), with a mean age of 59 years old. Most EBV-positive tumors were in gastric antrum. Two EBV-positive tumors (33.3%) were conventional adenocarcinomas, whereas four (66.7%) were classified as lymphoepithelioma-like carcinomas. EBV infection in gastric carcinomas was reported elsewhere in frequencies that range from 5.6% (Korea) up to 18% (Germany). In Brazil, a previous work found EBV infection in 4 of 80 (5%) gastric carcinomas, whereas another study found 4.7 and 11.2% of EBV-positive gastric carcinomas of Brazilians of Japanese origin or not, respectively. In the present study, the frequency of EBV-positive gastric carcinomas is similar to that reported in other series, and the clinicopathologic characteristics of these EBV-positive tumors are in agreement with the data in the literature.1707171

HYDROLOGICAL ASPECTS OF THE MESOSCALE ALPINE PROGRAMME: FINDINGS FROM FIELD EXPERIMENTS AND SIMULATIONS

Results of the Mesoscale Alpine Programme provide some answers to basic scientific questions of hydrological relevance posed in its scientific plan i.e: 1. verify the forecasting capabilities of a hydrological flood model, forced by the special measurements or coupled with advanced mesoscale atmospheric prediction models; 2. assess the role of the water storage in reservoirs on the runoff generation during floods in mountainous regions; 3. study the influence of soil moisture conditions prior to flood events in determining the production of runoff and investigate the capabilities and limitations of some soil moisture monitoring techniques over rugged terrain. Summary results from investigations in some areas in the southern (Toce, Ticino, Verzasca and Maggia watersheds) and northern (Ammer watershed) side of the Alps during the Mesoscale Alpine Programme and the 1999 Special Observing Period experiment are presented

Epstein-barr Virus Infection And Gastric Carcinoma In São Paulo, Brazil

Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and most people have serological evidence of previous viral infection at adult age. EBV is associated with infectious mononucleosis and human cancers, including some lymphomas and gastric carcinomas. Although EBV was first reported in lymphoepithelioma-like gastric carcinoma, the virus was also found in conventional adenocarcinomas. In the present study, 53 gastric carcinomas diagnosed in São Paulo State, Brazil, were evaluated for EBV infection by non-isotopic in situ hybridization with a biotinylated probe (Biotin-AGACACCGTCCTCACCACCC GGGACTTGTA) directed to the viral transcript EBER-I, which is actively expressed in EBV latently infected cells. EBV infection was found in 6 of 53 (11.32%) gastric carcinomas, mostly from male patients (66.7%), with a mean age of 59 years old. Most EBV-positive tumors were in gastric antrum. Two EBV-positive tumors (33.3%) were conventional adenocarcinomas, whereas four (66.7%) were classified as lymphoepithelioma-like carcinomas. EBV infection in gastric carcinomas was reported elsewhere in frequencies that range from 5.6% (Korea) up to 18% (Germany). In Brazil, a previous work found EBV infection in 4 of 80 (5%) gastric carcinomas, whereas another study found 4.7 and 11.2% of EBV-positive gastric carcinomas of Brazilians of Japanese origin or not, respectively. 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Fatores de influência no preço do milho no Brasil.

Introdução; Revisão Bibliográfica; O mercado da soja; Resultados e discussão; Conclusões

Two crystal forms of a hydrated 2:1 b-cyclodextrin× fluconazole complex: Single crystal x-ray structures, dehydration profiles, and conditions for their individual isolation

Inclusion complexes between cyclodextrins (CDs) and active pharmaceutical ingredients (APIs) have potential for pharmaceutical formulation. Since crystallization of a given complex may result in the isolation of multiple crystal forms, it is essential to characterize these forms with respect to their structures and physicochemical properties to optimize pharmaceutical candidate selection. Here, we report the preparation and characterization of two crystallographically distinct hydrated forms of an inclusion complex between β-cyclodextrin (β-CD) and the antifungal API fluconazole (FLU) as well as temperature–concentration conditions required for their individual isolation. Determination of crystal water contents was achieved using thermoanalytical methods. X-ray analyses revealed distinct structural differences between the triclinic (TBCDFLU, space group P1) and monoclinic (MBCDFLU, space group C2) crystal forms. Removal of the crystals from their mother liquors led to rapid dehydration of the MBCDFLU crystal, while the TBCDFLU crystal was stable, a result that could be reconciled with the distinct packing arrangements in the respective crystals. This study highlights (a) the importance of identifying possible multiple forms of a cyclodextrin×API complex and controlling the crystallization conditions, and (b) the need to characterize such crystal forms to determine the extent to which their physicochemical properties may differ