Efecto estacional sobre la composición de ácidos grasos de fosfolípidos y triacilgliceroles de machos de Capoeta umbla

The seasonal changes in muscle tissue, total lipids and fatty acid composition of phospholipids (PL), triacylglycerol (TAG), and phospholipid classes of male Capoeta umbla were investigated in this study. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS) were identified as the major phospholipids (PLs) in the muscle tissue (PS). Triacylglycerols showed high contents of MUFA, 14:0, 16:1n-7, 18:1n-9, 18:2n-6, and 18:3n-3; while phospholipids presented high contents of AA, DHA, and 18:0. Myristic acid, 16:1n-7, 18:1n-9, monounsaturated fatty acids (MUFA), linoleic acid (18:2n-6), and linolenic acid (18:3n-3) were shown to be present in larger concentrations in TAG than in PL classes. In PL classes, 16:0, 18:0, arachidonic acid (20:4n-6) (AA), eicosapentaenoic acid (EPA) (20:5n-3), docosahexaenoic acid (DHA) (22:6n-3) and polyunsaturated fatty acid (ΣPUFA) were higher than the TAG fraction. The fatty acid composition of total lipid and lipid classes (TAG and PL) were affected by the seasonal variations and lipid fraction.En este estudio se investigaron los cambios estacionales en el tejido muscular, la composición total de lípidos y ácidos grasos de fosfolípidos (PL), triacilglicerol (TAG) y clases de fosfolípidos de Capoeta umbla machos. Fosfatidilcolina (PC), fosfatidiletanolamina (PE), fosfatidilinositol (PI) y fosfatidilserina (PS) se identificaron como los principales fosfolípidos (PL) en el tejido muscular (PS). Los triacilgliceroles tenían un alto contenido de 14:0, de los MUFA 16:1n-7 y 18:1n-9 y de poliinsaturados 18:2n-6 y 18:3n-3, mientras que los fosfolípidos tenían un alto contenido de araquidónico (20:4n-6) (AA), docosahexaenoico (22:6n-3) (DHA) y 18:0. Se demostró que el ácido mirístico, los monoinsaturados (MUFA) 16:1n-7 y 18:1n-9, el ácido linoleico (18:2n-6) y el ácido linolénico (18:3n-3), están presentes en concentraciones superiores en TAG que en los PL. En las diferentes clases de PL, los ácidos 16:0, 18:0, AA, eicosapentaenoico (20:5n-3) (EPA), DHA y la suma de ácidos grasos poliinsaturados (ΣPUFA) fueron más altos que la fracción TAG. La composición de ácidos grasos de los lípidos totales y las clases de lípidos (TAG y PL) se vieron afectados por las variaciones estacionales y la fracción lipídica

Efecto estacional sobre el perfil de ácidos grasos de lípidos totales, fosfolípidos y triglicéridos de Mastacembelus mastacembelus (Lago Atatürk Dam, Turquía)

The present study investigated the lipid content and FA profiles of total lipids, phospholipids (PL) and triacylglycerols (TAG) of Mastacembelus mastacembelus from the Atatürk Dam Lake (Turkey). The results showed that the total lipid content of the dorsal muscle varied seasonally from 0.50% to 3.59%. Wide ranges of saturated fatty acids (SFAs) (36.21 to 50.52%) and polyunsaturated fatty acids (PUFAs) (25.22 to 42.02%) were found in the PL fraction. However, higher monounsaturated fatty acids (MUFAs) (37.63 to 45.07%) and SFA (34.71 to 38.82%) were found in TAG (triacylglycerol). The ratios of ω-3 to ω-6 PUFA ranged from 0.65 to 1.32 and 1.07 to 3.48 in PL (phospholipid) and TAG fraction, respectively. The results also showed that the major components were palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1ω-9), palmitoleic acid (C16:1ω-7), docosahexaenoic acid (DHA, C22:6ω-3), arachidonic acid (AA, C20:4ω-6) and docosapentaenoic acid (C22:5ω-3) in total lipid, C16:0, C18:1ω-9, AA and DHA in PL, C16:0, C16:1ω-7, C18:1ω-9, linoleic acid (LA, C18:2ω-6), and DHA in TAG extracted from the muscle of M. mastacembelus in all seasons.En el presente estudio se investigó el contenido de lípidos y los perfiles de ácidos grasos (FA) de lípidos totales, fosfolípidos (PL) y trigliceridos (TAG) de Mastacembelus mastacembelus del lago Atatürk Dam (Turquía). Los resultados mostraron que el contenido lipídico total del músculo dorsal varió estacionalmente de 0,50% a 3,59%. Se encontraron amplios rangos de ácidos grasos saturados (SFA) (36,21 a 50,52%) y ácidos grasos poliinsaturados (PUFA) (25,22 a 42,02%) en la fracción PL. Sin embargo, se encontraron ácidos grasos monoinsaturados (MUFA) más altos (37,63 a 45,07%) y SFA (34,71 a 38,82%) en TAG (triacilglicerol). Las relaciones de AGPI ω-3 a ω-6 variaron de 0,65 a 1,32 y de 1,07 a 3,48 en PL (fosfolípido) y fracción de TAG, respectivamente. Los resultados también muestran que los principales componentes fueron ácido palmítico (C16:0), esteárico (C18:0), oleico (C18:1 ω-9), palmitoleico (C16:1 ω-7), ácido docosahexaenoico (DHA), C22:6 ω-3), ácido araquidónico (AA, C20: 4 ?-6) y ácido docosapentaenoico (C22: 5 ω-3) en lípidos totales, C16:0, C18:1 ω-9, AA y DHA en PL, C16:0, C16:1 ω-7, C18:1 ω-9, ácido linoleico (LA, C18:2 ω-6) y DHA en el TAG extraído del músculo de M. mastacembelus en todas las estaciones

Seasonal effects of the fatty acid composition of phospholipid and triacylglycerol in the muscle and liver of male Salmo trutta macrostigma

The seasonal effects on the fatty acid composition of triacylglycerol (TAG) and phospholipid (PL) in the muscle and liver of male Salmo trutta macrostigma were determined using the gas chromatographic (GC) method. The fatty acid (FA) compositions of total lipid, PL and TAG fractions were determined in muscle and liver tissues of S. trutta macrostigma. The phospholipids contained a higher proportion of 16:0 compared to the TAG in the muscle tissue of S. trutta macrostigma. Docosahexaenoic acid (22:6 ω-3) and eicosapentaenoic acid (20:5 ω-3) contents were high in both muscle and liver tissues. The total lipid contents in the muscle and liver were 1.07-2.45 and 3.00-4.64%, respectively. S. trutta macrostigma is a rich source of ω-3 and ω-6, polyunsaturated fatty acids (PUFA) with numerous benefits to human health. © 2021 CSIC Consejo Superior de Investigaciones Cientificas. All rights reserved

Fatty acid compositions of colostrum and mature breast milk in Turkey (Mardin)

In this study, 25–25 milk samples (25 colostral and 25 mature) collected at different lactational stages were used to analyse the fatty acid composition of breast milk. A gas-chromatographic method was used to perform and analyse the transmethylation of total milk lipid extracts. The milk samples contained 20 different fatty acids. Palmitic acid (C16:0), stearic acid (C18:0), myristic acid (C14:0), oleic acid (C18:1n-9), and linoleic acid (LA, C18:2n-6) were the major components of total lipid, phospholipid (PL), and triacylglycerol (TAG) fractions. Colostrum had a lower percentage of polyunsaturated fatty acids (PUFAs), a higher percentage of saturated fatty acids (SFAs), and a lower level of eicosapentaenoic acid (EPA, C20:5 n-3) and docosahexaenoic acid (DHA, C22:6 n-3) in total lipid than mature milk. Triacylglycerol and PL fractions between colostrum and mature milk samples did not differ statistically

The protective role of vitamin E on the fatty acid composition of phospholipid structure in gill and liver tissues of Oreochromis niloticus exposed to deltamethrin

PubMedID: 22541830Deltamethrin is a commonly used pyrethroid pesticide. Vitamin E is a antioxidant that plays an important role in protecting cells against toxicity by inactivating free radicals generated following pesticides exposure. Therefore, in the present study, it was evaluated whether deltamethrin induced changes on the fatty acid composition of phospholipid in gill and liver tissues in Oreochromis niloticus and, the possible protective effect of vitamin E against deltamethrin was determined. Fish was fed with no pesticide+control diet, no pesticide+vitamin E-supplemented diet, 1.45 µg/l deltamethrin+control diet, 1.45 µg/l deltamethrin+vitamin E-supplemented diet for twenty days. Pesticide and diet quality made an impact on the fatty acid composition of phospholipid. In treatments of deltamethrin, group fed with control diet showed much greater damage in comparison with group fed with vitamin E supplemented diet. The results indicated that the deltamethrin led to an increase in the percentages of total SFAs (saturated fatty acids) and total MUFAs (monounsaturated fatty acids) and a decrease in total PUFAs (polyunsaturated fatty acids) in the gill tissues. However, coadministration of deltamethrin and vitamin E showed decrease in the percentages of saturated fatty acids and increase in the percentages of polyunsaturated fatty acids in the gill tissues. In group fed with control diet, deltamethrin led to a decrease in the percentage of SFAs and a increase in total MUFAs in the liver. Coadministration of deltamethrin and vitamin E showed increase in the percentages of polyunsaturated fatty acids in the liver tissues. These results have demonstrated that administration of vitamin E along with deltamethrin decreases the peroxidation of unsaturated fatty acids and thus protects the cell membranes. © 2012 Elsevier Inc.DUBAP-10-FF-12This study was financially supported by Dicle University, Research Fund Project (Project no: DUBAP-10-FF-12)

Factors effecting influenza vaccination uptake among health care workers: a multi-center cross-sectional study

Background: The present study aimed to identify factors affecting vaccination against influenza among health professionals. Methods: We used a multi-centre cross-sectional design to conduct an online self-administered questionnaire with physicians and nurses at state and foundation university hospitals in the south-east of Turkey, between 1 January 2015 and 1 February 2015. The five participating hospitals provided staff email address lists filtered for physicians and nurses. The questionnaire comprised multiple choice questions covering demographic data, knowledge sources, and Likert-type items on factors affecting vaccination against influenza. The target response rate was 20 %. Results: In total, 642 (22 %) of 2870 health professionals (1220 physicians and 1650 nurses) responded to the questionnaire. Participants' mean age was 29.6 +/- 9.2 years (range 17-62 years); 177 (28.2 %) were physicians and 448 (71.3 %) were nurses. The rate of regular vaccination was 9.2 % (15.2 % for physicians and 8.2 % for nurses). Increasing age, longer work duration in health services, being male, being a physician, working in an internal medicine department, having a chronic disease, and living with a person over 65 years old significantly increased vaccination compliance (p < 0.05). We found differences between vaccine compliant and non-compliant groups for expected benefit from vaccination, social influences, and personal efficacy (p < 0.05). Univariate analysis showed differences between the groups in perceptions of personal risks, side effects, and efficacy of the vaccine (p < 0.05). Multivariate analysis found that important factors influencing vaccination behavior were work place, colleagues' opinions, having a chronic disease, belief that vaccination was effective, and belief that flu can be prevented by natural ways. Conclusion: Numerous factors influence health professionals' decisions about influenza vaccination. Strategies to increase the ratio of vaccination among physicians and nurses should consider all of these factors to increase the likelihood of success