190 research outputs found
Polyphenols journey through blood-brain barrier towards neuronal protection
Age-related complications such as neurodegenerative disorders are increasing and remain cureless.
The possibility of altering the progression or the development of these multifactorial diseases through
diet is an emerging and attractive approach with increasing experimental support. We examined
the potential of known bioavailable phenolic sulfates, arising from colonic metabolism of berries, to
infuence hallmarks of neurodegenerative processes. In silico predictions and in vitro transport studies
across blood-brain barrier (BBB) endothelial cells, at circulating concentrations, provided evidence for
diferential transport, likely related to chemical structure. Moreover, endothelial metabolism of these
phenolic sulfates produced a plethora of novel chemical entities with further potential bioactivies.
Pre-conditioning with phenolic sulfates improved cellular responses to oxidative, excitotoxicity and
infammatory injuries and this attenuation of neuroinfammation was achieved via modulation of
NF-κB pathway. Our results support the hypothesis that these small molecules, derived from dietary
(poly)phenols may cross the BBB, reach brain cells, modulate microglia-mediated infammation and
exert neuroprotective efects, with potential for alleviation of neurodegenerative diseases.info:eu-repo/semantics/publishedVersio
Efeito de reguladores de crescimento no potencial osmótico de tomateiro (Lycopersicon esculentum Mill. cv. Angela)
This research deals with the effects of exogenous growth regulators on leaf osmotic potential of tomato cultivar "Angela". To study the influence of growth substances on osmotic potentia, (2-chloroethyl) trimethylammonium chloride (CCC) at concentration of 2,000 ppm, succinic acid-2,2-dirnethylhydrazide (Alar) (3,000 ppm), and potassium gibberellate (Gibrel) (100 ppm) were applied. The leaf osmotic potential established everyday during six days showed that plants treated with Gibrel presented lower (negative) osmotic potential. CCC promoted better equilibrium in the osmotic potential of tomato plants. Leaves of tomato cultivar "Angela" presented higher osmotic potential in the morning in relation to leaves collected in the afternoon.A importância do estudo do potencial osmótico das plantas deve-se ao fato deste parâmetro ser considerado o principal componente do potencial hídrico, refletindo o equilíbrio de água na planta. Determinou-se o potencial osmótico foliar do tomateiro (Lycopersicon esculentum Mill. cv. "Angela") através de osmômetro de precisão. As plantas haviam sido tratadas com cloreto de (2-cloroetil) trimetilamônio (CCC) 2000 ppm, ácido succínico-2,2-dimetilhidrazida (Alar) 3000 ppm e giberelato de potássio (Gibrel) 100 ppm, além do controle. Verificou-se que o Gibrel promoveu uma redução no potencial osmótico; sendo que Alar provocou flutuações no potencial do tomateiro "Angela". O CCC causou maior equilíbrio no potencial osmótico foliar do tomateiro. Observou-se que as folhas da cultivar "Angela'' mostraram potencial osmótico mais alto pela manhã com relação ao período da tarde
4D gravity on a non-BPS bent dilatonic brane
We investigate the localization of metastable four-dimensional gravity around
a bent dilatonic brane, embedded into a five-dimensional space, that exists
only up to distances sufficiently small compared to a crossover scale. Far from
such scale, five-dimensional effects strongly deviate the Newtonian potential.
We study this effect by considering localization of massive gravity on a
non-BPS bent dilatonic 3-brane solution of a five-dimensional supergravity. Our
results show that the cosmological constant on the bent brane controls the size
of the crossover scale. For sufficiently small positive cosmological constant,
that is in accord with the present observations, the crossover scale becomes
very large.Comment: 7 pages, version to appear in JCA
Dissecting the sugarcane expressed sequence tag (SUCEST) database: unraveling flower-specific genes
There are almost 260,000 independent clones sequenced from the 5? end in the Sugarcane Expressed Sequence Tag (SUCEST) database, which have been obtained from 37 cDNA libraries prepared from different tissues. This large number of expressed sequence tags (ESTs) provides an opportunity, unprecedented in plants, to perform ?digital differential screening? on selected cDNA libraries. In general, the frequency of a particular EST correlates with transcript accumulation in the tissues from which the cDNA libraries were constructed, so it is possible to compare the whole transcriptome from different tissues using computer-assisted analysis of an EST database. In our research we analyzed sugarcane ESTs according to tissue expression and identified more than 1,000 putative flower-specific genes. The fact that using this technique we were able to identify sugarcane homologues of several genes previously described as pollen-specific justifies this method of assessing tissue specificity. In addition, ESTs similar to genes specific to reproductive organs were detected e.g. a sugarcane gene encoding a meiotic protein essential for assembly of the synaptonemal complex and normal synapsis. This approach also allowed the identification of many flower-specific anonymous sequences that are good candidates for being novel genes involved in plant reproduction. This paper describes the analysis of the gene expression levels of 24 EST clusters during flower development using a ?digital northern blot? constructed from direct EST counts made on the non-normalized sugarcane cDNA libraries.Existem quase 260.000 clones independentes, seqüenciados a partir da extremidade 5?, no banco de dados do SUCEST (Sugarcane Expressed Sequence Tag), os quais foram obtidos a partir de 37 bibliotecas de cDNA preparadas de diferentes tecidos. Este grande número de etiquetas de sequências expressas (ESTs) fornece uma oportunidade, sem precedentes em plantas, de realizar um ?digital differential screening? em bibliotecas de cDNA selecionadas. Geralmente, a frequência de um determinado EST está correlacionada ao acúmulo de transcritos nos tecidos dos quais as bibliotecas de cDNA foram construídas, e desta forma, é possível comparar o transcriptoma completo de diferentes tecidos, usando uma análise computacional de um banco de dados de ESTs. Em nossa pesquisa, analisamos os ESTs de cana-de-açúcar de acordo com sua expressão tecidual e identificamos mais de 1.000 putativos genes específicos de flor. O fato de que usando esta técnica fomos capazes de identificar homológos em cana-de-açúcar, de vários genes previamente descritos como específicos de pólen, sustenta este método de estimar especificidade tecidual. Além disto, ESTs com similaridade a genes específicos de órgãos reprodutivos foram revelados, como por exemplo, o gene que codifica uma proteína meiótica essencial para a montagem do complexo sinaptonêmico e sinapse normal. Esta abordagem também permitiu a identificação de muitas sequências anônimas, específicas de flor, que são boas candidatas para novos genes envolvidos com a reprodução de plantas. Este trabalho descreve a análise dos níveis de expressão gênica de 24 clusters de ESTs, durante o desenvolvimento floral, usando um ?northern blot digital? construído a partir da contagem direta dos ESTs das bibliotecas não-normalizadas de cDNAs de cana-de-açúcar.7784Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq
Thrips species (Insecta: Thysanoptera) associated with flowers in a restinga fragment in northeastern Brazil
Phylogenetic and pathotype analysis of Escherichia coli swine isolates from Southern Brazil
Helminths of the Lizard Salvator merianae (Squamata, Teiidae) in the Caatinga, Northeastern Brazil
Superradiance in the BTZ black hole with Robin boundary conditions
We show the existence of superradiant modes of massive scalar fields propagating in BTZ black holes when certain Robin boundary conditions, which never include the commonly considered Dirichlet boundary conditions, are imposed at spatial infinity. These superradiant modes are defined as those solutions whose energy flux across the horizon is towards the exterior region. Differently from rotating, asymptotically flat black holes, we obtain that notall modes which grow up exponentially in time are superradiant; for some of these, the growth is sourced by a bulk instability of AdS(3), triggered by the scalar field with Robin boundary conditions, rather than by energy extraction from the BTZ black hole. Thus, this setup provides an example wherein Bosonic modes with low frequency are pumping energy into, rather than extracting energy from, a rotating black hole. (C) 2018 The Authors. Published by Elsevier B.V.publishe
Dissecting the sugarcane expressed sequence tag (SUCEST) database: unraveling flower-specific genes
- …