INITIAL EVALUATION OF CYCLIC ADENOSINE MONOPHOSPHATE ENZYME IMMUNOASSAY FOR USE WITH CRANE SEMEN SAMPLES

The management of ex situ and in situ populations of many wildlife species requires detailed knowledge of a species’ reproductive biology. For species such as cranes, where artificial insemination is a critical component of ex situ management strategies, understanding normal sperm function is especially important. Previous research has shown that captive cranes exhibit highly variable production and quality of semen samples produced by individual males and high levels of variation of cell concentration and motility across different species. Cyclic adenosine monophosphate (cyclic AMP) has been implicated in regulating sperm function, such as cell motility, and may affect an individual’s ability to successfully fertilize. Here we demonstrate the feasibility of an enzyme-linked immunosorbent assay (ELISA) for measuring cyclic AMP produced by crane sperm to facilitate future research into its role in sperm function and fertilization

EFFECTS OF RELEASE TECHNIQUES ON PARENT-REARED WHOOPING CRANES IN THE EASTERN MIGRATORY POPULATION

Reintroduction of an Eastern Migratory Population (EMP) of whooping cranes (Grus americana) in the United States by release of captive-reared individuals began in 2001. As of 2020, the EMP has approximately 21 breeding pairs and has had limited recruitment of wild-hatched individuals, thus captive-reared juveniles continue to be released into breeding areas in Wisconsin to maintain the population. We investigated the effects of release techniques on survival, behavior, site fidelity, and conspecific associations of 42 captive-parent-reared whooping cranes released during 2013-2019 into the EMP. Individuals were monitored intensively post-release, then as a part of a long-term monitoring program, locational, behavioral, and habitat use data were collected and analyzed. Most cranes roosted in water post-release; however, we documented 4 parent-reared cranes roosting on dry land. Most cranes eventually associated with other whooping cranes; however, juveniles released near single adult cranes were less likely to associate with other whooping cranes during their first migration or winter than juveniles released near other types of whooping crane pairs or groups. Parent-reared and costume-reared whooping cranes had similar rates of survival 1 year post-release (69.0% and 64.4%, respectively). The highest risk of mortality was within the first 100 days post-release, and the leading known causes of death were predation and impact trauma due to powerline or vehicle collisions. Both costume- and parent-reared cranes had strong fidelity to release sites. We advise releasing parent-reared cranes near pairs or groups of whooping cranes and taking measures to reduce the risk of mortality during the immediate period after release (e.g., predator aversion training, marking powerlines)

What makes re-finding information difficult? A study of email re-finding

Re-nding information that has been seen or accessed before is a task which can be relatively straight-forward, but often it can be extremely challenging, time-consuming and frustrating. Little is known, however, about what makes one re-finding task harder or easier than another. We performed a user study to learn about the contextual factors that influence users' perception of task diculty in the context of re-finding email messages. 21 participants were issued re-nding tasks to perform on their own personal collections. The participants' responses to questions about the tasks combined with demographic data and collection statistics for the experimental population provide a rich basis to investigate the variables that can influence the perception of diculty. A logistic regression model was developed to examine the relationships be- tween variables and determine whether any factors were associated with perceived task diculty. The model reveals strong relationships between diculty and the time lapsed since a message was read, remembering when the sought-after email was sent, remembering other recipients of the email, the experience of the user and the user's ling strategy. We discuss what these findings mean for the design of re-nding interfaces and future re-finding research

How can health systems be strengthened to control and prevent an Ebola outbreak? a narrative review

The emergence and re-emergence of infectious diseases are now more than ever considered threats to public health systems. There have been over 20 outbreaks of Ebola in the past 40 years. Only recently, the World Health Organization has declared a public health emergency of international concern (PHEIC) in West Africa, with a projected estimate of 1.2 million deaths expected in the next 6 months. Ebola virus is a highly virulent pathogen, often fatal in humans and non-human primates. Ebola is now a great priority for global health security and often becomes fatal if left untreated. This study employed a narrative review. Three major databases MEDLINE, EMBASE, and Global Health were searched using both ‘text-words’ and ‘thesaurus terms’. Evidence shows that low- and middle-income countries (LMICs) are not coping well with the current challenges of Ebola, not only because they have poor and fragile systems but also because there are poor infectious disease surveillance and response systems in place. The identification of potential cases is problematic, particularly in the aspects of contact tracing, infection control, and prevention, prior to the diagnosis of the case. This review therefore aims to examine whether LMICs’ health systems would be able to control and manage Ebola in future and identifies two key elements of health systems strengthening that are needed to ensure the robustness of the health system to respond effectively

Social Determinants of Smoking Behavior: The Healthy Twin Study, Korea

Objectives: The purpose of this study was to identify any influence of socioeconomic status on smoking and smokingcessation in a situation where genetic factors are controlled.Methods: The sample for this study was 2502 members of the twins and families cohort who participated in the KoreanHealthy Twins Study from 2005 to 2009. Groups of brothers or sisters, including twins and fraternal twins, were comparedin terms of smoking and smoking cessation behaviors according to differences in socioeconomic status and gender.Results: In a situation with complete control of genetic factors, results showed that the daily smoking amount, cumulativesmoking amount, and dependence on nicotine decreased with higher-status occupations, and the rate of smoking andamount of cumulative smoking decreased with higher levels of education. Regarding smoking cessation behavior, a higherlevel of education was associated with a lower smoking cessation rate, and no significant gender differences were found.Conclusions: Environmental factors had a stronger influence on smoking behavior than did genetic factors. Geneticfactors had greater influence on smoking cessation than did environmental factors; however, this requires verification infurther studies.Key words: Smoking, Smoking cessation, Social class, TwinsOAIID:oai:osos.snu.ac.kr:snu2012-01/102/0000040632/2SEQ:2PERF_CD:SNU2012-01EVAL_ITEM_CD:102USER_ID:0000040632ADJUST_YN:NEMP_ID:A077602DEPT_CD:902CITE_RATE:0FILENAME:2.Social Determinants of Smoking Behavior.pdfDEPT_NM:보건학과EMAIL:[email protected]:

High-temporal resolution fluvial sediment source fingerprinting with uncertainty: a Bayesian approach

This contribution addresses two developing areas of sediment fingerprinting research. Specifically, how to improve the temporal resolution of source apportionment estimates whilst minimizing analytical costs and, secondly, how to consistently quantify all perceived uncertainties associated with the sediment mixing model procedure. This first matter is tackled by using direct X-ray fluorescence spectroscopy (XRFS) and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) analyses of suspended particulate matter (SPM) covered filter papers in conjunction with automatic water samplers. This method enables SPM geochemistry to be quickly, accurately, inexpensively and non-destructively monitored at high-temporal resolution throughout the progression of numerous precipitation events. We then employed a Bayesian mixing model procedure to provide full characterization of spatial geochemical variability, instrument precision and residual error to yield a realistic and coherent assessment of the uncertainties associated with source apportionment estimates. Applying these methods to SPM data from the River Wensum catchment, UK, we have been able to apportion, with uncertainty, sediment contributions from eroding arable topsoils, damaged road verges and combined subsurface channel bank and agricultural field drain sources at 60- and 120-minute resolution for the duration of five precipitation events. The results presented here demonstrate how combining Bayesian mixing models with the direct spectroscopic analysis of SPM-covered filter papers can produce high-temporal resolution source apportionment estimates that can assist with the appropriate targeting of sediment pollution mitigation measures at a catchment level

Mental, social, and physical well-being in New Hampshire, Oregon, and Washington, 2010 Behavioral Risk Factor Surveillance System: implications for public health research and practice related to Healthy People 2020 foundation health measures on well-being

BACKGROUND: Well-being is now accepted as one of four cross-cutting measures in gauging progress for Healthy People 2020. This shift to population indicators of well-being redresses notions of health that have focused on absence of illness (negative health) as a primary or sufficient indicator of positive functioning. The purpose of this study was to estimate mental, social, and physical well-being in three US states using new measures piloted on the 2010 Behavioral Risk Factor Surveillance Survey System (BRFSS). Baseline estimates were provided for states overall, and within states for demographic subgroups, those with chronic health conditions or disabilities, and those with behavioral risk factors. METHODS: Ten validated questions designed to assess mental (e.g., satisfaction with life, satisfaction with life domains, happiness), physical (e.g., satisfaction with energy level), and social dimensions (e.g., frequency of social support) of well-being were selected with state input for inclusion on BRFSS. 18,622 individuals responded to the BRFSS surveys administered by New Hampshire (N = 3,139), Oregon (N = 2,289), and Washington (N = 13,194). Multivariate adjusted proportions of positive responses to well-being items were examined. RESULTS: After adjustment for confounders, about 67% of adults in these states had high levels of well-being, including >80% reporting experiencing happiness. Most adults were satisfied with their work, neighborhood, and education, but significant differences were seen in subgroups. Well-being differed by demographic characteristics such as marital status, health behaviors, chronic conditions, and disability status, with those who reported a disability and smokers consistently experiencing the worst well-being. CONCLUSIONS: Well-being is accepted as one of four cross-cutting measures in gauging progress for Healthy People 2020. Well-being differs by important sociodemographic factors and health conditions (e.g., age, employment, smoking, disability status). These findings provide baseline estimates for the three states to use in gauging improvements in well-being and can serve as a model for other state-level or national surveillance systems. These findings also assist states in identifying vulnerable subgroups who may benefit from potential interventions such as those in the National Prevention Strategy that focus on enhancing well-being where such disparities exist

A robust system for RNA interference in the chicken using a modified microRNA operon

AbstractRNA interference (RNAi) provides an effective method to silence gene expression and investigate gene function. However, RNAi tools for the chicken embryo have largely been adapted from vectors designed for mammalian cells. Here we present plasmid and retroviral RNAi vectors specifically designed for optimal gene silencing in chicken cells. The vectors use a chicken U6 promoter to express RNAs modelled on microRNA30, which are embedded within chicken microRNA operon sequences to ensure optimal Drosha and Dicer processing of transcripts. The chicken U6 promoter works significantly better than promoters of mammalian origin and in combination with a microRNA operon expression cassette (MOEC), achieves up to 90% silencing of target genes. By using a MOEC, we show that it is also possible to simultaneously silence two genes with a single vector. The vectors express either RFP or GFP markers, allowing simple in vivo tracking of vector delivery. Using these plasmids, we demonstrate effective silencing of Pax3, Pax6, Nkx2.1, Nkx2.2, Notch1 and Shh in discrete regions of the chicken embryonic nervous system. The efficiency and ease of use of this RNAi system paves the way for large-scale genetic screens in the chicken embryo

Clinical Presentations and Neurodevelopmental Outcomes of Perinatal Stroke in Preterm and Term Neonates: A Case Series

Perinatal stroke in neonates can lead to disability in later life. However, its etiology and prognosis are poorly understood. The aim of this study was to describe clinical presentations and neurodevelopmental outcomes of our case series of perinatal stroke in Korea. Thirteen term and preterm neonates who were diagnosed with perinatal stroke in two university hospitals from March 2003 to March 2007 were enrolled. Seven term and 6 preterm neonates were diagnosed with perinatal stroke, based on the brain MRI findings. Perinatal stroke presented with seizure (4/13), perinatal distress (3/13) in term neonates, whereas stroke in preterm neonates did not present with noticeable clinical symptoms. Only one neonate had positive thrombophilic test (homozygous C677T polymorphism for MTHFR). Ten neonates had infarctions in the territory of the middle cerebral artery (MCA), and 3 neonates had borderzone infarctions between the anterior cerebral artery and MCA. Neurodevelopmental outcome was abnormal in 4 neonates. Infarction in MCA main branch or posterior limb of internal capsule showed an abnormal neurodevelopmental outcome. Our study is the first systematic study of perinatal stroke in Korea, and shows its clinical presentations and neurodevelopmental outcomes. The population-based study on incidence and prognosis of perinatal stroke in Korea is required in the future