132 research outputs found
Urginea capitata Baker - the berg slangkop, its toxic effects on ruminants, with a brief botanical survey of area
The green leaves proved poisonous when fed, mixed with lucerne, to cattle. The symptoms preceding death were " tucked up" appearance, sunken eyeballs, loss of appetite and profuse diarrhoea with blood stained faeces. The minimum fatal dose for an average sized animal is given as 6-8 lb. of the green material.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201
Diminished Virulence Of A Sar-lagr- Mutant Of Staphylococcus Aureus In The Rabbit Model Of Endocarditis
Microbial pathogenicity in Staphylococcus aureus is a complex process involving a number of virulence genes that are regulated by global regulatory systems including sar and agr. To evaluate the roles of these two loci in virulence, we constructed sar-lagr- mutants of strains RN6390 and RN450 and compared their phenotypic profiles to the corresponding single sar- and agr- mutants and parents. The secretion of all hemolysins was absent in the sar-lagr- mutants while residual β-hemolysin activity remained in single agr- mutants. The fibronectin binding capacity was significantly diminished in both single sar- mutants and double mutants when compared with parents while the reduction in fibrinogen binding capacity in the double mutants was modest. In the rabbit endocarditis model, there was a significant decrease in both infectivity rates and intravegetation bacterial densities with the double mutant as compared to the parent (RN6390) at 103-106 CFU inocula despite comparable levels of early bacteremia among various challenge groups. Notably, fewer bacteria in the double mutant group adhered to valvular vegetations at 30 min after challenge (106 CFU) than the parent group. These studies suggest that both the sar and agr loci are involved in initial valvular adherence, intravegetation persistence and multiplication of S. aureus in endocarditis.94518151822Waldvogel, F.A., Staphylococcus aureus (1985) Principles and Practice of Infectious Diseases, pp. 1097-1116. , G. L. Mandell, R. G. Douglas, Jr., and J. E. Bennett, editors. John Wiley & Sons, New YorkNeu, H.C., The crisis in antibiotic resistance (1992) Science (Wash. DC), 257, pp. 1064-1072Cohen, M.L., Epidemiology of drug resistance: Implications for a post-antibiotic era (1992) Science (Wash. DC), 257, pp. 1050-1055Easmon, C.S.F., Adlam, C., (1983) Staphylococci and Staphylococcal Infections, pp. 705-740. , Academic Press, New YorkCheung, A.L., Krishnan, M., Jaffe, E.A., Fischetti, V.A., Fibrinogen acts as a bridging molecule in the adherence of Staphylococcus aureus to cultured human endothelial cells (1991) J. Clin. Invest., 87, pp. 2236-2245Herrmann, M., Vaudaux, P.E., Pittet, D., Auckenthaler, R., Lew, P.D., Schumacher Perdreau, F., Peters, G., Waldvogel, F.A., Fibronectin, fibrinogen, and laminin act as mediators of adherence of clinical staphylococcal isolates to foreign material (1988) J. Infect. Dis., 158, pp. 693-701Kuypers, J.M., Proctor, R.A., Reduced adherence to traumatized rat heart valves by a low-fibronectin-binding mutant of Staphylococcus aureus (1989) Infect. Immun., 57, pp. 2306-2312DeRita, V.J., Mekalanos, J.J., Genetic regulation of bacterial virulence (1989) Annu. Rev. Genet., 23, pp. 455-482Kornblum, J., Kreiswirth, B., Projan, S.J., Ross, H., Novick, R.P., Agr: A polycistronic locus regulating exoprotein synthesis in Staphylococcus aureus (1990) Molecular Biology of the Staphylococci, pp. 373-402. , R. P. Novick, editor. VCH Publishers, New YorkNixon, B.T., Ronson, C.W., Ausubel, R.M., Two component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and btrC (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 7850-7854Smeltzer, M.S., Hart, M.E., Iandolo, J.J., Phenotypic characterization of xpr, a global regulator of extracellular virulence factors in Staphylococcus aureus (1993) Infect. Immun., 61, pp. 919-925Cheung, A.L., Koomey, J.M., Butler, C.A., Projan, S.J., Fischetti, V.A., Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 6462-6466Cheung, A.L., Yeaman, M., Bayer, A.S., The role of the sar locus of Staphylococcus aureus in the induction of endocarditis in rabbits (1994) Infect. Immun., 62, pp. 1719-1725Vandenesch, F., Kornblum, J., Novick, R.P., A temporal signal, independent of agr, is required for hla but not spa transcription in Staphylococcus aureus (1991) J. Bacteriol., 173, pp. 6313-6320Cheung, A.L., Ying, P., Regulation of α and β hemolysins by the sar locus of Staphylococcus aureus (1994) J. Bacteriol., 176, pp. 580-585Rescei, P., Kreiswirth, B., O'Reilly, M., Schlievert, P., Gruss, A., Novick, R.P., Regulation of exoprotein gene expression in Staphylococcus aureus by agr (1986) Mol. & Gen. Genet., 202, pp. 58-61Fröman, G., Switalski, L., Speziale, P., Höök, M., Isolation and characterization of a fibronectin receptor from Staphylococcus aureus (1987) J. Biol. Chem., 262, pp. 6564-6571Lantz, M., Allen, R.D., Bounelis, P., Switalski, L.M., Höök, M., Bacteroides gingivalis and Bacteroides intermedius recognize different sites on human fibrinogen (1990) J. Bacteriol., 172, pp. 716-726Yeaman, M.R., Sullam, P.M., Dazin, P.F., Norman, D.C., Bayer, A.S., Characterization of Staphylococcus aureus-platelet binding by quantitative flow cytometric analysis (1992) J. Infect. Dis., 166, pp. 65-73Clawson, C.C., White, J.G., Herzberg, M.C., Platelet interaction with bacteria. VI. Contrasting the role of fibrinogen and fibronectin (1980) Am. J. Hematol., 9, pp. 43-53Yeaman, M.R., Norman, D.C., Bayer, A.S., Staphylococcus aureus susceptibility to thrombin-induced platelet microbicidal protein is independent of platelet adherence and aggregation in vitro (1992) Infect. Immun., 60, pp. 2368-2374Sullam, P.M., Payan, D.G., Dazin, P.F., Valone, F.H., Binding of viridans group streptococci to human platelets: A quantitative analysis (1990) Infect. Immun., 58, pp. 3802-3806Yeaman, M.R., Puentes, S.M., Norman, D.C., Bayer, A.S., Partial characterization and staphylocidal activity of thrombin-induced platelet microbicidal protein (1992) Infect. Immun., 60, pp. 1202-1209Kornblum, J., Projan, S.J., Moghazeh, S.L., Novick, R., A rapid method to quantitate non-labeled RNA species in bacterial cells (1988) Gene, 63, pp. 75-85Maniatis, T., Fritsch, E.F., Sambrook, J., (1989) Molecular Cloning, a Laboratory Manual, , Cold Spring Harbor Laboratory, Cold Spring Harbor, NYDurack, D.T., Beeson, P.B., Experimental bacterial endocarditis. I. Colonization of a sterile vegetation (1972) Br. J. Exp. Pathol., 53, pp. 44-49Scheld, W.M., Valone, J.A., Sande, M.A., Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets and fibrin (1978) J. Clin. Invest., 61, pp. 1394-1404Herzberg, M.C., Gong, K., McMarlane, G.D., Phenotypic characterization of Streptococcus sanguis virulence factors associated with bacterial endocarditis (1990) Infect. Immun., 58, pp. 515-522Cheung, A.L., Fischetti, V.A., The role of fibrinogen in staphylococcal adherence to catheters in vitro (1990) J. Infect. Dis., 161, pp. 1177-1186Herrmann, M., Vaudaux, P.E., Pittet, D., Auckenthaler, R., Lew, P.D., Schumacher Perdreau, F., Peters, G., Waldvogel, F.A., Fibronectin, fibrinogen, and laminin act as mediators of adherence of clinical staphylococcal isolates to foreign material (1988) J. Infect. Dis., 158, pp. 693-701Cheung, A.L., Projan, S.J., Cloning and sequencing of sarA: A gene required for the expression of agr (1994) J. Bacteriol., 176, pp. 4168-4172Scheld, W.M., Strunk, R.W., Balian, G., Calderone, R.A., Microbial adhesion to fibronectin in vitro correlates with production of endocarditis in rabbits (1985) Proc. Soc. Exp. Biol. Med., 180, pp. 474-482Durack, D.T., Beeson, P.B., Pathogenesis of infective endocarditis (1980) Infective Endocarditis, pp. 1-53. , S. H. Rahimtoola, editor. Grune and Stratton, Inc., New YorkJaffe, E., Cell biology of endothelial cells (1987) Hum. Pathol., 18, pp. 234-239Lopes, J.D., Reis, M.D., Bretani, R.R., Presence of laminin receptors in Staphylococcus aureus (1985) Science (Wash. DC), 229, pp. 275-277Patti, J.M., Bremell, T., Krajewska-Paetrasik, D., Adelnour, A., Tarkowski, A., Ryden, C., Höök, M., The Staphylococcus aureus collagen adhesin is a virulence determinant in experimental septic arthritis Infect. Immun., 62, pp. 152-161Herrmann, M., Suchard, S.J., Boxer, L.A., Waldvogel, F.A., Lew, P.D., Thrombospondin binds to Staphylococcus aureus and promotes staphylococcal adhesion to surfaces (1991) Infect. Immun., 59, pp. 279-288Bhakdi, S., Muhly, M., Mannhardt, U., Hugo, F., Klapettek, K., Muller-Eckhardt, C., Roka, L., Staphylococcal α-toxin promotes blood coagulation via attack on human platelets (1988) J. Exp. Med., 168, pp. 527-542Suttorp, N., Hessz, T., Seeger, W., Wilke, A., Koob, R., Lutz, F., Drenckhahn, D., Bacterial exotoxins and endothelial permeability for water and albumin in vitro (1988) Am. J. Physiol., 255, pp. C368Novick, R.P., Genetic systems in staphylococci (1991) Methods Enzymol., 204, pp. 587-636O'Reilly, M., De Azavedo, J.C.S., Kennedy, S., Foster, T.J., Inactivation of the alpha-hemolysin of Staphylococcus aureus 8325-4 by site directed mutagenesis and studies on the expression of its haemolysins (1986) Microb. 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Resonant driving of magnetization precession in a ferromagnetic layer by coherent monochromatic phonons
We realize resonant driving of the magnetization precession by monochromatic phonons in a thin ferromagnetic layer embedded into a phononic Fabry-Pérot resonator. A femtosecond laser pulse excites resonant phonon modes of the structure in the 10−40 GHz frequency range. By applying an external magnetic field, we tune the precession frequency relative to the frequency of the phonons localized in the cavity and observe an enormous increase in the amplitude of the magnetization precession when the frequencies of free magnetization precession and phonons localized in the cavity are equal
Quantitative predictions on auxin-induced polar distribution of PIN proteins during vein formation in leaves
The dynamic patterning of the plant hormone auxin and its efflux facilitator
the PIN protein are the key regulator for the spatial and temporal organization
of plant development. In particular auxin induces the polar localization of its
own efflux facilitator. Due to this positive feedback auxin flow is directed
and patterns of auxin and PIN arise. During the earliest stage of vein
initiation in leaves auxin accumulates in a single cell in a rim of epidermal
cells from which it flows into the ground meristem tissue of the leaf blade.
There the localized auxin supply yields the successive polarization of PIN
distribution along a strand of cells. We model the auxin and PIN dynamics
within cells with a minimal canalization model. Solving the model analytically
we uncover an excitable polarization front that triggers a polar distribution
of PIN proteins in cells. As polarization fronts may extend to opposing
directions from their initiation site we suggest a possible resolution to the
puzzling occurrence of bipolar cells, such we offer an explanation for the
development of closed, looped veins. Employing non-linear analysis we identify
the role of the contributing microscopic processes during polarization.
Furthermore, we deduce quantitative predictions on polarization fronts
establishing a route to determine the up to now largely unknown kinetic rates
of auxin and PIN dynamics.Comment: 9 pages, 4 figures, supplemental information included, accepted for
publication in Eur. Phys. J.
Study of KS KL Coupled Decays and KL -Be Interactions with the CMD-2 Detector at VEPP-2M Collider
The integrated luminosity about 4000 inverse nanobarn of around phi meson
mass ( 5 millions of phi mesons) has been collected with the CMD-2 detector at
the VEPP-2M collider. A latest analysis of the KS KL coupled decays based on 30
% of available data is presented in this paper.
The KS KL pairs from phi meson decays were reconstructed in the drift chamber
when both kaons decayed into two charged particles. From a sample of 1423
coupled decays a selection of candidates to the CP violating KL into pi+ pi-
decay was performed. CP violating decays were not identified because of the
domination of events with a KL regenerating at the Be beam pipe into KS and a
background from KL semileptonic decays.
The regeneration cross section of 110 MeV/c KL mesons was found to be 53 +-
17 mb in agreement with theoretical expectations. The angular distribution of
KS mesons after regeneration and the total cross section of KL for Be have been
measured.Comment: 14 pages, 8 figure
Qualidade do solo em sistemas de manejo avaliada pela dinâmica da matéria orgânica e atributos relacionados
Semeadura direta na produção do milho em agricultura de sequeiro na região Nordeste do Brasil
Aplicação aérea de fungicidas com diferentes equipamentos e volumes sobre a produtividade, qualidade de grãos e controle de doenças em arroz irrigado
Detector Description and Performance for the First Coincidence Observations between LIGO and GEO
For 17 days in August and September 2002, the LIGO and GEO interferometer
gravitational wave detectors were operated in coincidence to produce their
first data for scientific analysis. Although the detectors were still far from
their design sensitivity levels, the data can be used to place better upper
limits on the flux of gravitational waves incident on the earth than previous
direct measurements. This paper describes the instruments and the data in some
detail, as a companion to analysis papers based on the first data.Comment: 41 pages, 9 figures 17 Sept 03: author list amended, minor editorial
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