Trypanosoma brucei brucei: differences in the nuclear chromatin of bloodstream forms and procyclic culture forms

Nucleosome filaments of two stages of the life-cycle of Trypanosoma brucei brucei, namely bloodstream forms and procyclic culture forms, were investigated by electron microscopy. Chromatin of bloodstream forms showed a salt-dependent condensation. The level of condensation was higher than that shown by chromatin from procyclic culture forms, but 30 nm fibres as formed in rat liver chromatin preparations were not found. Analysis of histones provided new evidence for the existence of H1-like proteins, which comigrated in the region of the core histones in SDS-PAGE and in front of the core histones in Triton acid urea gels. Differences were found between the H1-like proteins of the two trypanosome stages as well as between the core histones in their amount, number of bands and banding pattern. It can be concluded that T. b. brucei contains a full set of histones, including H1-like proteins, and that the poor condensation of its chromatin is not due to the absence of H1, but most probably due to histone-DNA interaction being weak. It is obvious that structural and functional differences of the chromatin exist not only between T. b. brucei and higher eukaryotes, but also between various stages of the life-cycle of the parasite. It is therefore not adequate to investigate the chromatin only of the procyclic culture forms as a model for all stages of the life-cycle of T. b. bruce

Deuteron form factor measurements at low momentum transfers

A precise measurement of the elastic electron-deuteron scattering cross section at four-momentum transfers of 0.24 fm(-1) \u3c = Q \u3c = 2.7 fm(-1) has been performed at the Mainz Microtron. In this paper we describe the utilized experimental setup and the necessary analysis procedure to precisely determine the deuteron charge form factor from these data. Finally, the deuteron charge radius r(d) can be extracted from an extrapolation of that form factor to Q(2) = 0

Recoil polarization and beam-recoil double polarization measurement of \eta electroproduction on the proton in the region of the S_{11}(1535) resonance

The beam-recoil double polarization P_{x'}^h and P_{z'}^h and the recoil polarization P_{y'} were measured for the first time for the p(\vec{e},e'\vec{p})\eta reaction at a four-momentum transfer of Q^2=0.1 GeV^2/c^2 and a center of mass production angle of \theta = 120^\circ at MAMI C. With a center of mass energy range of 1500 MeV < W < 1550 MeV the region of the S_{11}(1535) and D_{13}(1520) resonance was covered. The results are discussed in the framework of a phenomenological isobar model (Eta-MAID). While P_{x'}^h and P_{z'}^h are in good agreement with the model, P_{y'} shows a significant deviation, consistent with existing photoproduction data on the polarized-target asymmetry.Comment: 4 pages, 1 figur

Search for Light Gauge Bosons of the Dark Sector at the Mainz Microtron

A new exclusion limit for the electromagnetic production of a light U(1) gauge boson {\gamma}' decaying to e^+e^- was determined by the A1 Collaboration at the Mainz Microtron. Such light gauge bosons appear in several extensions of the standard model and are also discussed as candidates for the interaction of dark matter with standard model matter. In electron scattering from a heavy nucleus, the existing limits for a narrow state coupling to e^+e^- were reduced by nearly an order of magnitude in the range of the lepton pair mass of 210 MeV/c^2 < m_e^+e^- < 300 MeV/c^2. This experiment demonstrates the potential of high current and high resolution fixed target experiments for the search for physics beyond the standard model.Comment: 4 pages, 7 figure

Reply to Comment on "High-Precision Determination of the Electric and Magnetic Form Factors of the Proton"

In arXiv:1108.3058v1 [nucl-ex], Arrington criticizes the Coulomb corrections we applied in the analysis of high precision form factor data (see Phys.Rev.Lett.105:242001, 2010, arXiv:1007.5076v3 [nucl-ex]). We show, by comparing different calculations cited in the Comment, that the criticism of the Comment neglects the large uncertainty of "more modern" TPE corrections. This uncertainty has also been seen in recent polarized measurements. We rerun our analysis using one of these calculations. The results show that the Comment exaggerates the quantitative effect at small Q^2.Comment: 1 page, 2 figure, To appear as a Reply Comment in Physical Review Letter

Measurement of polarization-transfer to bound protons in carbon and its virtuality dependence

We measured the ratio $P_{x}/P_{z}$ of the transverse to longitudinal components of polarization transferred from electrons to bound protons in $^{12}\mathrm{C}$ by the $^{12}\mathrm{C}(\vec{e},e'\vec{p})$ process at the Mainz Microtron (MAMI). We observed consistent deviations from unity of this ratio normalized to the free-proton ratio, $(P_{x}/P_{z})_{^{12}\mathrm{C}}/(P_{x}/P_{z})_{^{1}\mathrm{H}}$, for both $s$- and $p$-shell knocked out protons, even though they are embedded in averaged local densities that differ by about a factor of two. The dependence of the double ratio on proton virtuality is similar to the one for knocked out protons from $^{2}\mathrm{H}$ and $^{4}\mathrm{He}$, suggesting a universal behavior. It further implies no dependence on average local nuclear density

High-precision determination of the electric and magnetic form factors of the proton

New precise results of a measurement of the elastic electron-proton scattering cross section performed at the Mainz Microtron MAMI are presented. About 1400 cross sections were measured with negative four-momentum transfers squared up to Q^2=1 (GeV/c)^2 with statistical errors below 0.2%. The electric and magnetic form factors of the proton were extracted by fits of a large variety of form factor models directly to the cross sections. The form factors show some features at the scale of the pion cloud. The charge and magnetic radii are determined to be r_E=0.879(5)(stat.)(4)(syst.)(2)(model)(4)(group) fm and r_M=0.777(13)(stat.)(9)(syst.)(5)(model)(2)(group) fm.Comment: 5 pages, 2 figures, published in Phys. Rev. Lett. v3: added references, updated text, color figure

A Large-Scale FPGA-Based Trigger and Dead-Time Free DAQ System for the Kaos Spectrometer at MAMI

The Kaos spectrometer is maintained by the A1 collaboration at the Mainz Microtron MAMI with a focus on the study of (e,e'K^+) coincidence reactions. For its electron-arm two vertical planes of fiber arrays, each comprising approximately 10 000 fibers, are operated close to zero degree scattering angle and in close proximity to the electron beam. A nearly dead-time free DAQ system to acquire timing and tracking information has been installed for this spectrometer arm. The signals of 144 multi-anode photomultipliers are collected by 96-channel front-end boards, digitized by double-threshold discriminators and the signal time is picked up by state-of-the-art F1 time-to-digital converter chips. In order to minimize background rates a sophisticated trigger logic was implemented in newly developed Vuprom modules. The trigger performs noise suppression, signal cluster finding, particle tracking, and coincidence timing, and can be expanded for kinematical matching (e'K^+) coincidences. The full system was designed to process more than 4 000 read-out channels and to cope with the high electron flux in the spectrometer and the high count rate requirement of the detectors. It was successfully in-beam tested at MAMI in 2009.Comment: Contributed to 17th IEEE Real Time Conference (RT10), Lisbon, 24-28 May 201

The electric and magnetic form factors of the proton

The paper describes a precise measurement of electron scattering off the proton at momentum transfers of $0.003 \lesssim Q^2 \lesssim 1$\ GeV$^2$. The average point-to-point error of the cross sections in this experiment is $\sim$ 0.37%. These data are used for a coherent new analysis together with all world data of unpolarized and polarized electron scattering from the very smallest to the highest momentum transfers so far measured. The extracted electric and magnetic form factors provide new insight into their exact shape, deviating from the classical dipole form, and of structure on top of this gross shape. The data reaching very low $Q^2$ values are used for a new determination of the electric and magnetic radii. An empirical determination of the Two-Photon-Exchange (TPE) correction is presented. The implications of this correction on the radii and the question of a directly visible signal of the pion cloud are addressed.Comment: 38 pages, 20 figures. Updated data files. PRC versio