16,342 research outputs found
Characterization of the Noise in Secondary Ion Mass Spectrometry Depth Profiles
The noise in the depth profiles of secondary ion mass spectrometry (SIMS) is
studied using different samples under various experimental conditions. Despite
the noise contributions from various parts of the dynamic SIMS process, its
overall character agrees very well with the Poissonian rather than the Gaussian
distribution in all circumstances. The Poissonian relation between the measured
mean-square error (MSE) and mean can be used to describe our data in the range
of four orders. The departure from this relation at high counts is analyzed and
found to be due to the saturation of the channeltron used. Once saturated, the
detector was found to exhibit hysteresis between rising and falling input flux
and output counts.Comment: 14 pages, 4 postscript figures, to appear on J. Appl. Phy
Equilibrium binding energies from fluctuation theorems and force spectroscopy simulations
Brownian dynamics simulations are used to study the detachment of a particle
from a substrate. Although the model is simple and generic, we attempt to map
its energy, length and time scales onto a specific experimental system, namely
a bead that is weakly bound to a cell and then removed by an optical tweezer.
The external driving force arises from the combined optical tweezer and
substrate potentials, and thermal fluctuations are taken into account by a
Brownian force. The Jarzynski equality and Crooks' fluctuation theorem are
applied to obtain the equilibrium free energy difference between the final and
initial states. To this end, we sample non--equilibrium work trajectories for
various tweezer pulling rates. We argue that this methodology should also be
feasible experimentally for the envisioned system. Furthermore, we outline how
the measurement of a whole free energy profile would allow the experimentalist
to retrieve the unknown substrate potential by means of a suitable
deconvolution. The influence of the pulling rate on the accuracy of the results
is investigated, and umbrella sampling is used to obtain the equilibrium
probability of particle escape for a variety of trap potentials.Comment: 21 pages, 11 figures, To appear in Soft Matte
Standardization and application of microsatellite markers for variety identification in tomato and wheat
The present study is part of a EU project that aims to demonstrate the technical viability of STMS markers for variety identification. As examples two important European crop species, tomato and wheat were chosen. Initially, about 30-40 STMS markers were used to identify a set of 20 good markers per crop and to standardise the methodology and the interpretation of the results in different laboratories. Several systems were used for the detection of STMS polymorphisms. The selected STMS markers are being tested on 500 varieties of each species and databases are being constructed. The first comparisons of data generated by the different laboratories revealed a high degree of agreement. The causes of discrepancies between duplicate samples analysed in different laboratories and precautions to prevent them, are discussed
Obtaining Stiffness Exponents from Bond-diluted Lattice Spin Glasses
Recently, a method has been proposed to obtain accurate predictions for
low-temperature properties of lattice spin glasses that is practical even above
the upper critical dimension, . This method is based on the observation
that bond-dilution enables the numerical treatment of larger lattices, and that
the subsequent combination of such data at various bond densities into a
finite-size scaling Ansatz produces more robust scaling behavior. In the
present study we test the potential of such a procedure, in particular, to
obtain the stiffness exponent for the hierarchical Migdal-Kadanoff lattice.
Critical exponents for this model are known with great accuracy and any
simulations can be executed to very large lattice sizes at almost any bond
density, effecting a insightful comparison that highlights the advantages -- as
well as the weaknesses -- of this method. These insights are applied to the
Edwards-Anderson model in with Gaussian bonds.Comment: corrected version, 10 pages, RevTex4, 12 ps-figures included; related
papers available a http://www.physics.emory.edu/faculty/boettcher
The Lombard intelligibility benefit of native and non-native speech for native and non-native listeners
Speech produced in noise (Lombard speech) is more intelligible than speech produced in quiet (plain speech). Previous research on the Lombard intelligibility benefit focused almost entirely on how native speakers produce and perceive Lombard speech. In this study, we investigate the size of the Lombard intelligibility benefit of both native (American-English) and non-native (native Dutch) English for native and non-native listeners (Dutch and Spanish). We used a glimpsing metric to measure the energetic masking potential of speech, which predicted that both native and non-native Lombard speech could withstand greater amounts of masking to a similar extent, compared to plain speech. In an intelligibility experiment, native English, Spanish, and Dutch listeners listened to the same words, mixed with noise. While the non-native listeners appeared to benefit more from Lombard speech than the native listeners did, each listener group experienced a similar benefit for native and non-native Lombard speech. Energetic masking, as captured by the glimpsing metric, only accounted for part of the Lombard benefit, indicating that the Lombard intelligibility benefit does not only result from a shift in spectral distribution. Despite subtle native language influences on non-native Lombard speech, both native and non-native speech provides a Lombard benefit
Protein-mediated dethreading of a biotin-functionalised pseudorotaxane
In this article, we describe the synthesis of new biotin-functionalised naphthalene derivatives 3 and 4 and their complexation behaviour with avidin and neutravidin using a range of analytical techniques. We have shown using 2-(4prime or minute-hydroxyazobenzene)benzoic acid displacement and ITC experiments{,} that compounds 3 and 4 have the propensity to form reasonably high-affinity bioconjugates with avidin and neutravidin. We have also demonstrated using 1H NMR{,} UV-vis and fluorescence spectroscopy that the naphthalene moiety of 3 and 4 facilitates the formation of pseudorotaxane-like structures with 1 in water. We have then investigated the ability of avidin and neutravidin to modulate the complexation between 1 and 3 or 4. UV-vis and fluorescence spectroscopy has shown that in both cases the addition of the protein disrupts complexation between the naphthalene moieties of 3 and 4 with 1
Deep MOS Spectroscopy of NGC 1316 Globular Clusters
The giant elliptical galaxy NGC 1316 is the brightest galaxy in the Fornax
cluster, and displays a number of morphological features that might be
interpreted as an intermediate age merger remanent (3 Gyr). Based on the
idea that globular clusters systems (GCS) constitute genuine tracers of the
formation and evolution of their host galaxies, we conducted a spectroscopic
study of approximately 40 globular clusters (GCs) candidates associated with
this interesting galaxy. We determined ages, metallicities, and
-element abundances for each GC present in the sample, through the
measurement of different Lick indices and their subsequent comparison with
simple stellar populations models (SSPs).Comment: 5 pages, 3 figure
NASA-JSC antenna near-field measurement system
Work was completed on the near-field range control software. The capabilities of the data processing software were expanded with the addition of probe compensation. In addition, the user can process the measured data from the same computer terminal used for range control. The design of the laser metrology system was completed. It provides precise measruement of probe location during near-field measurements as well as position data for control of the translation beam and probe cart. A near-field range measurement system was designed, fabricated, and tested
Recommended from our members
Trait anxiety but not state anxiety during critical Illness was associated with anxiety and depression over 6 months after ICU
Objective:
To determine the association between anxiety during critical illness and symptoms of anxiety and depression over 6 months after ICU discharge in survivors of intensive care treatment.
Design:
Longitudinal study.
Setting:
One closed mixed ICU in an adult tertiary hospital in Brisbane, Australia.
Patients:
Participants (n = 141) were adults (≥ 8 yr), admitted to ICU for at least 24 hours, able to communicate either verbally or nonverbally, understand English, and open their eyes spontaneously or in response to voice.
Interventions: None.
Measurements and Main Results:
The outcomes of symptoms of anxiety and depression over 6 months after ICU discharge were assessed using the Hospital Anxiety Depression Scale. The primary variable of interest was anxiety during critical illness. Two components of anxiety (state and trait) were assessed during critical illness using the Faces Anxiety Scale and the trait component of the State-Trait Anxiety Inventory. Perceived social support, cognitive functioning, and posttraumatic stress symptoms were also assessed using standardized instruments. Clinical and demographic data were obtained from patients and medical records. Participants were followed up in hospital wards and at 3 and 6 months after ICU discharge. During ICU treatment, 81 of the 141 participants (57%) reported moderate to severe levels of state anxiety. Of the 92 participants who completed the surveys at the 6-month follow-up, 26 participants (28%) reported symptoms of anxiety and 21 (23%) symptoms of depression. Symptoms of anxiety and depression were strongly correlated in this cohort of survivors. Trait anxiety was significantly associated with both anxiety and depression symptoms over time; however, state anxiety was not associated with either outcome. Participants who reported post-ICU memories of intra-ICU anxiety were significantly more anxious during recovery over 6 months. Cognitive functioning and posttraumatic stress symptoms were both significantly associated with anxiety and depression symptoms over time.
Conclusion:
Symptoms of anxiety and depression are a significant issue for general ICU survivors. Trait anxiety was significantly associated with adverse emotional outcomes over 6 months after ICU discharge. There was also a significant relationship between post-ICU memories of intra-ICU anxiety and anxiety during recovery. Interventions to reduce anxiety during critical illness need to be considered and evaluated for their longer term benefits for survivors of critical illness
- …