Uncertainties of size measurements in electron microscopy characterization of nanomaterials in foods

Electron microscopy is a recognized standard tool for nanomaterial characterization, and recommended by the European Food Safety Authority for the size measurement of nanomaterials in food. Despite this, little data have been published assessing the reliability of the method, especially for size measurement of nanomaterials characterized by a broad size distribution and/or added to food matrices. This study is a thorough investigation of the measurement uncertainty when applying electron microscopy for size measurement of engineered nanomaterials in foods. Our results show that the number of measured particles was only a minor source of measurement uncertainty for nanomaterials in food, compared to the combined influence of sampling, sample preparation prior to imaging and the image analysis. The main conclusion is that to improve the measurement reliability, care should be taken to consider replications and matrix removal prior to sample preparation

Spinodal decomposition in alkali feldspar studied by atom probe tomography

We used atom probe tomography to complement electron microscopy for the investigation of spinodal decomposition in alkali feldspar. To this end, gem-quality alkali feldspar of intermediate composition with a mole fraction of aK=0.43 of the K end-member was prepared from Madagascar orthoclase by ion-exchange with (NaK)Cl molten salt. During subsequent annealing at 550∘C and close to ambient pressure the ion-exchanged orthoclase unmixed producing a coherent lamellar intergrowth of Na-rich and K-rich lamellae. The chemical separation was completed, and equilibrium Na–K partitioning between the different lamellae was attained within four days, which was followed by microstructural coarsening. After annealing for 4 days, the wavelength of the lamellar microstructure was ≈17nm and it increased to ≈30nm after annealing for 16 days. The observed equilibrium compositions of the Na-rich and K-rich lamellae are in reasonable agreement with an earlier experimental determination of the coherent solvus. The excess energy associated with compositional gradients at the lamellar interfaces was quantified from the initial wavelength of the lamellar microstructure and the lamellar compositions as obtained from atom probe tomography using the Cahn–Hilliard theory. The capability of atom probe tomography to deliver quantitative chemical compositions at nm resolution opens new perspectives for studying the early stages of exsolution. In particular, it helps to shed light on the phase relations in nm scaled coherent intergrowth

A foundation for provitamin A biofortification of maize: genome-wide association and genomic prediction models of carotenoid levels.

Efforts are underway for development of crops with improved levels of provitamin A carotenoids to help combat dietary vitamin A deficiency. As a global staple crop with considerable variation in kernel carotenoid composition, maize (Zea mays L.) could have a widespread impact. We performed a genome-wide association study (GWAS) of quantified seed carotenoids across a panel of maize inbreds ranging from light yellow to dark orange in grain color to identify some of the key genes controlling maize grain carotenoid composition. Significant associations at the genome-wide level were detected within the coding regions of zep1 and lut1, carotenoid biosynthetic genes not previously shown to impact grain carotenoid composition in association studies, as well as within previously associated lcyE and crtRB1 genes. We leveraged existing biochemical and genomic information to identify 58 a priori candidate genes relevant to the biosynthesis and retention of carotenoids in maize to test in a pathway-level analysis. This revealed dxs2 and lut5, genes not previously associated with kernel carotenoids. In genomic prediction models, use of markers that targeted a small set of quantitative trait loci associated with carotenoid levels in prior linkage studies were as effective as genome-wide markers for predicting carotenoid traits. Based on GWAS, pathway-level analysis, and genomic prediction studies, we outline a flexible strategy involving use of a small number of genes that can be selected for rapid conversion of elite white grain germplasm, with minimal amounts of carotenoids, to orange grain versions containing high levels of provitamin A

Low-extinction windows in the inner Galactic Bulge

We built K band extinction maps in the area of two candidate low-extinction windows in the inner Bulge: W0.2-2.1 at (l,b) = (0.25o,-2.15o), and W359.4-3.1 at (l,b) = (359.40o,-3.10o). We employed JHKs photometry from the 2MASS Point Source Catalog. Extinction values were determined by fitting the upper giant branch found in the present 2MASS Ks x J-Ks diagrams to a de-reddened bulge stellar population reference giant branch. We tested the method on the well known Baade's and Sgr I windows: the 2MASS mean extinction values in these fields agreed well with those of previous studies. We confirm the existence of low-extinction windows in the regions studied, as local minima in the A_K maps reaching A_K values about 2 standard deviations below the mean values found in the neighbouring areas. Schlegel et al.'s (1998) FIR extinction maps, which integrate dust contributions throughout the Galaxy, are structurally similar to those derived with 2MASS photometry in the two studied windows. We thus conclude that the dust clouds affecting the 2MASS and FIR maps in these directions are basically the same and are located on foreground of the bulk of bulge stars. However, the A_K absolute values differ significantly. In particular, the FIR extinction values for W359.4-3.1 are a factor ~1.45 larger than those derived from the 2MASS photometry. Possible explanations of this effect are discussed. The lower Galactic latitudes of the low-extinction windows W359.4-3.1 and W0.2-2.1, as compared to Baade's Window, make them promising targets for detailed studies of more central bulge regions.Comment: 8 pages, 10 figures, LaTeX, aa.cls. To appear in Astron. & Astroph., in pres

The Frequency of Barred Spiral Galaxies in the Near-IR

We have determined the fraction of barred galaxies in the H-band for a statistically well-defined sample of 186 spirals drawn from the Ohio State University Bright Spiral Galaxy survey. We find 56% of our sample to be strongly barred at H, while another 16% is weakly barred. Only 27% of our sample is unbarred in the near-infrared. The RC3 and the Carnegie Atlas of Galaxies both classify only about 30% of our sample as strongly barred. Thus strong bars are nearly twice as prevalent in the near-infrared as in the optical. The frequency of genuine optically hidden bars is significant, but lower than many claims in the literature: 40% of the galaxies in our sample that are classified as unbarred in the RC3 show evidence for a bar in the H-band, while for the Carnegie Atlas this fraction is 66%. Our data reveal no significant trend in bar fraction as a function of morphology in either the optical or H-band. Optical surveys of high redshift galaxies may be strongly biased against finding bars, as bars are increasingly difficult to detect at bluer rest wavelengths.Comment: LaTeX with AASTeX style file, 23 pages with 6 figures. Accepted for publication in The Astronomical Journal (Feb. 2000

Simultaneous Optical Model Analyses of Elastic Scattering, Breakup, and Fusion Cross Section Data for the 6^{6}He + 209^{209}Bi System at Near-Coulomb-Barrier Energies

Based on an approach recently proposed by us, simultaneous $\chi^{2}$-analyses are performed for elastic scattering, direct reaction (DR) and fusion cross sections data for the $^{6}$He+$^{209}$Bi system at near-Coulomb-barrier energies to determine the parameters of the polarization potential consisting of DR and fusion parts. We show that the data are well reproduced by the resultant potential, which also satisfies the proper dispersion relation. A discussion is given of the nature of the threshold anomaly seen in the potential