Comparison of Saturated Hydraulic Conductivity Measurement Methods for a Glacial-Till Soil

Hydraulic conductivity is the single most important hydraulic parameter for flow and transport-related phenomena in soil, but the results from different measuring methods vary under different field conditions. To evaluate the performance of four in situ saturated hydraulic conductivity (Ks) measuring methods, Ks measurements were made at four depths (15, 30, 60, and 90 cm) and five locations on a glacial-till soil of Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll)-Clarion (fine-loamy, mixed, mesic Typic Hapludoll) association. The four in situ methods were: (i) Guelph permeameter, (ii) velocity permeameter, (iii) disk permeameter, and (iv) double-tube method. The Ks was also determined in the laboratory on undisturbed soil cores collected from all the five sites and four depths. The Guelph permeameter method gave the lowest Ks values, possibly because of small sample size, whereas the disk permeameter and double-tube methods gave maximum values for Ks with minimum variability, possibly because of large sample size. Maximum variability in Ks values for soil cores at shallow depths may have occurred because of the presence or absence of open-ended macropores. Estimates of Ks, however, are most comparable for the velocity permeameter and the laboratory method using a constant-head permeameter

Temporal Dynamics of Preferential Flow to a Subsurface Drain

We conducted a sequential tracer leaching study on a 24.4 by 42.7 m field plot to investigate the temporal behavior of chemical movement to a 1.2-m deep field drain during irrigation and subsequent rainfall events over a 14-d period. The herbicides atrazine [6-chloroN-ethyl-N′-(1-methylethyl)-1,3,5-triazine-2,4-diamine], and alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide] along with the conservative tracer Br were applied to a 1-m wide strip, offset 1.5 m laterally from a subsurface drain pipe, immediately before an 11.3-h long, 4.2-mm h−1 irrigation. Three additional conservative tracers, pentafluorobenzoate (PF), o-trifluoromethylbenzoate (TF), and difluorobenzoate (DF) were applied to the strip during the irrigation at 2-h intervals. Breakthrough of Br and the two herbicides occurred within the first 2-h of irrigation, indicating that a fraction of the solute transport was along preferential flow paths. Retardation and attenuation of the herbicides indicated that there was interaction between the chemicals and the soil lining the preferential pathways. The conservative tracers applied during the later stages of irrigation arrived at the subsurface drain much faster than tracers applied earlier. The final tracer, applied 6 h after the start of irrigation (DF), took only 15 min and 1 mm of irrigation water to travel to the subsurface drain. Model simulations using a two-dimensional, convective, and dispersive numerical model without an explicit preferential flow component failed to reproduce Br tracer concentrations in the drain effluent, confirming the importance of preferential flow. This study showed that preferential flow in this soil is not a uniform process during a leaching event

Antimicrobial and toxicological studies of some metal complexes of 4-methylpiperazine-1-carbodithioate and phenanthroline mixed ligands

A few mixed ligand transition metal carbodithioate complexes of the general formula [M(4-MPipzcdt)x(phen)y]Y (M = Mn(II), Co(II), Zn(II); 4-MPipzcdt = 4-methylpiperazine-1-carbodithioate; phen = 1,10-phenanthroline; x = 1 and y = 2 when Y = Cl; x = 2 and y = 1 when Y = nil) were synthesized and screened for their antimicrobial activity against Candida albicans, Escherichia coli, Pseudomonas aeruginosa,Staphylococcus aureus and Enterococcusfaecalis by disk diffusion method. All the complexes exhibited prominent antimicrobial activity against tested pathogenic strains with the MIC values in the range &lt;8-512 &mu;gmL-1. The complexes [Mn(4-MPipzcdt)2(phen)] and [Co(4-MPipzcdt)(phen)2]Cl inhibited the growth of Candida albicans at a concentration as low as 8 &micro;gmL-1.The complexes were also evaluated for their toxicity towards human transformed rhabdomyosarcoma cells (RD cells). Moderate cell viability of the RD cells was exhibited against the metal complexes.<br /

MLPerf Inference Benchmark

Machine-learning (ML) hardware and software system demand is burgeoning. Driven by ML applications, the number of different ML inference systems has exploded. Over 100 organizations are building ML inference chips, and the systems that incorporate existing models span at least three orders of magnitude in power consumption and five orders of magnitude in performance; they range from embedded devices to data-center solutions. Fueling the hardware are a dozen or more software frameworks and libraries. The myriad combinations of ML hardware and ML software make assessing ML-system performance in an architecture-neutral, representative, and reproducible manner challenging. There is a clear need for industry-wide standard ML benchmarking and evaluation criteria. MLPerf Inference answers that call. In this paper, we present our benchmarking method for evaluating ML inference systems. Driven by more than 30 organizations as well as more than 200 ML engineers and practitioners, MLPerf prescribes a set of rules and best practices to ensure comparability across systems with wildly differing architectures. The first call for submissions garnered more than 600 reproducible inference-performance measurements from 14 organizations, representing over 30 systems that showcase a wide range of capabilities. The submissions attest to the benchmark's flexibility and adaptability.Comment: ISCA 202

Urinary MicroRNA Profiling in the Nephropathy of Type 1 Diabetes

Background: Patients with Type 1 Diabetes (T1D) are particularly vulnerable to development of Diabetic nephropathy (DN) leading to End Stage Renal Disease. Hence a better understanding of the factors affecting kidney disease progression in T1D is urgently needed. In recent years microRNAs have emerged as important post-transcriptional regulators of gene expression in many different health conditions. We hypothesized that urinary microRNA profile of patients will differ in the different stages of diabetic renal disease. Methods and Findings: We studied urine microRNA profiles with qPCR in 40 T1D with >20 year follow up 10 who never developed renal disease (N) matched against 10 patients who went on to develop overt nephropathy (DN), 10 patients with intermittent microalbuminuria (IMA) matched against 10 patients with persistent (PMA) microalbuminuria. A Bayesian procedure was used to normalize and convert raw signals to expression ratios. We applied formal statistical techniques to translate fold changes to profiles of microRNA targets which were then used to make inferences about biological pathways in the Gene Ontology and REACTOME structured vocabularies. A total of 27 microRNAs were found to be present at significantly different levels in different stages of untreated nephropathy. These microRNAs mapped to overlapping pathways pertaining to growth factor signaling and renal fibrosis known to be targeted in diabetic kidney disease. Conclusions: Urinary microRNA profiles differ across the different stages of diabetic nephropathy. Previous work using experimental, clinical chemistry or biopsy samples has demonstrated differential expression of many of these microRNAs in a variety of chronic renal conditions and diabetes. Combining expression ratios of microRNAs with formal inferences about their predicted mRNA targets and associated biological pathways may yield useful markers for early diagnosis and risk stratification of DN in T1D by inferring the alteration of renal molecular processes. © 2013 Argyropoulos et al

Interpreting the Evolution of the Size - Luminosity Relation for Disk Galaxies from Redshift 1 to the Present

A sample of very high resolution cosmological disk galaxy simulations is used to investigate the evolution of galaxy disk sizes back to redshift 1 within the Lambda CDM cosmology. Artificial images in the rest frame B band are generated, allowing for a measurement of disk scale lengths using surface brightness profiles as observations would, and avoiding any assumption that light must follow mass as previous models have assumed. We demonstrate that these simulated disks are an excellent match to the observed magnitude - size relation for both local disks, and for disks at z=1 in the magnitude/mass range of overlap. We disentangle the evolution seen in the population as a whole from the evolution of individual disk galaxies. In agreement with observations, our simulated disks undergo roughly 1.5 magnitudes/arcsec^2 of surface brightness dimming since z=1. We find evidence that evolution in the magnitude - size plane varies by mass, such that galaxies with M* > 10^9 M_sun undergo more evolution in size than luminosity, while dwarf galaxies tend to evolve potentially more in luminosity. The disks grow in such a way as to stay on roughly the same stellar mass - size relation with time. Finally, due to an evolving stellar mass - SFR relation, a galaxy at a given stellar mass (or size) at z=1 will reside in a more massive halo and have a higher SFR, and thus a higher luminosity, than a counterpart of the same stellar mass at z=0.Comment: Version resubmitted to ApJ, after referee's comment

Regulation of mammary gland branching morphogenesis by the extracellular matrix and its remodeling enzymes.

A considerable body of research indicates that mammary gland branching morphogenesis is dependent, in part, on the extracellular matrix (ECM), ECM-receptors, such as integrins and other ECM receptors, and ECM-degrading enzymes, including matrix metalloproteinases (MMPs) and their inhibitors, tissue inhibitors of metalloproteinases (TIMPs). There is some evidence that these ECM cues affect one or more of the following processes: cell survival, polarity, proliferation, differentiation, adhesion, and migration. Both three-dimensional culture models and genetic manipulations of the mouse mammary gland have been used to study the signaling pathways that affect these processes. However, the precise mechanisms of ECM-directed mammary morphogenesis are not well understood. Mammary morphogenesis involves epithelial 'invasion' of adipose tissue, a process akin to invasion by breast cancer cells, although the former is a highly regulated developmental process. How these morphogenic pathways are integrated in the normal gland and how they become dysregulated and subverted in the progression of breast cancer also remain largely unanswered questions

The Wnt/β-catenin pathway regulates growth and maintenance of colonospheres

<p>Abstract</p> <p>Background</p> <p>Recent evidence suggests that epithelial cancers, including colorectal cancer are driven by a small sub-population of self-renewing, multi-potent cells termed cancer stem cells (CSCs) which are thought to be responsible for recurrence of cancer. One of the characteristics of CSCs is their ability to form floating spheroids under anchorage-independent conditions in a serum-free defined media. The current investigation was undertaken to examine the role of Wnt/β-catenin pathway in regulating the growth and maintenance of colonospheres. Human colon cancer cells HCT-116 (p53 wild type; <it>K-ras </it>mutant), HCT-116 (p53 null; <it>K-ras </it>mutant) and HT-29 (p53 mutant) were used.</p> <p>Results</p> <p>Colonospheres formed <it>in vitro </it>exhibited higher expression of colon CSCs markers LGR5, CD44, CD166 and Musashi-1 along with putative CSC marker EpCAM, compared to the corresponding parental cancer cells and also exhibit the ability to form spheroids under extreme limiting dilution, indicating the predominance of CSCs in colonospheres. Colonospheres formed by HCT-116 cells show over 80% of the cells to be CD44 positive, compared to ≤ 1% in the corresponding parental cells. Additionally, colonospheres showed reduced membrane bound β-catenin but had increased levels of total β-catenin, cyclin-D1 and c-myc and down regulation of axin-1 and phosphorylated β-catenin. Increased expression of β-catenin was associated with a marked transcriptional activation of TCF/LEF. The latter was greatly decreased following down regulation of β-catenin by the corresponding siRNA, leading to a marked reduction in CD44 positive cells as well as colonospheres formation. In contrast, upregulation of c-myc, a down-stream effector of TCF/LEF greatly augmented the formation of colonospheres.</p> <p>Conclusion</p> <p>Our data suggest that colonospheres formed by colon cancer cell lines are highly enriched in CSCs and that Wnt/β-catenin pathway plays a critical role in growth and maintenance of colonospheres.</p

IL-17 Expression in the Time Course of Acute Anti-Thy1 Glomerulonephritis

Background Interleukin-17 (IL-17) is a new pro-inflammatory cytokine involved in immune response and inflammatory disease. The main source of IL-17 is a subset of CD4+ T-helper cells, but is also secreted by non-immune cells. The present study analyzes expression of IL-17 in the time course of acute anti- thy1 glomerulonephritis and the role of IL-17 as a potential link between inflammation and fibrosis. Methods Anti-thy1 glomerulonephritis was induced into male Wistar rats by OX-7 antibody injection. After that, samples were taken on days 1, 5, 10 (matrix expansion phase), 15 and 20 (resolution phase). PBS-injected animals served as controls. Proteinuria and histological matrixes score served as the main markers for disease severity. In in vitro experiments, NRK-52E cells were used. For cytokine expressions, mRNA and protein levels were analyzed by utilizing RT-PCR, in situ hybridization and immunofluorescence. Results Highest IL-17 mRNA-expression (6.50-fold vs. con; p<0.05) was found on day 5 after induction of anti-thy1 glomerulonephritis along the maximum levels of proteinuria (113 ± 13 mg/d; p<0.001), histological glomerular-matrix accumulation (82%; p<0.001) and TGF-β1 (2.2-fold; p<0.05), IL-6 mRNA expression (36-fold; p<0.05). IL-17 protein expression co-localized with the endothelial cell marker PECAM in immunofluorescence. In NRK-52E cells, co-administration of TGF-β1 and IL-6 synergistically up-regulated IL-17 mRNA 4986-fold (p<0.001). Conclusions The pro-inflammatory cytokine IL-17 is up-regulated in endothelial cells during the time course of acute anti-thy1 glomerulonephritis. In vitro, NRK-52E cells secrete IL-17 under pro-fibrotic and pro-inflammatory conditions