Writing the Experiences and (Corporeal) Knowledges of Women of Color into Educational Studies: A Colloquium

In this colloquium, we share collaborative ideas that came about during a weekend retreat. We center our discussions on Chicana and Black feminisms and Womanism, specifically addressing how women of color feminisms inspire us; imagining/defining space; tensions within our sisterhoods; transforming (inner)coloniality by embracing our lived herstories; and how Chicana and Black feminisms and Womanism transform educational studies. We leave readers with hopes for our-selves, our fields, our sisters, and for the world. While not exact tellings of our pláticas during our retreat, we capture and share the essence of burning questions, ideas, and hopes that arose for us when thinking and talking about women of color feminisms and educational studies

Spectroscopic confirmation of the planetary nebula nature of PM1-242, PM1-318 and PM1-333 and morphological analysis of the nebulae

We present intermediate resolution long-slit spectra and narrow-band Halpha, [NII] and [OIII] images of PM1-242, PM318 and PM1-333, three IRAS sources classified as possible planetary nebulae. The spectra show that the three objects are true planetary nebulae and allow us to study their physical properties; the images provide a detailed view of their morphology. PM1-242 is a medium-to-high-excitation (e.g., HeII4686/Hbeta ~0.4; [NII]6584/Halpha ~0.3) planetary nebula with an elliptical shape containing [NII] enhanced point-symmetric arcs. An electron temperature [Te([SIII])] of ~10250 K and an electron density [Ne([SII])] of ~2300 cm-3 are derived for PM1-242. Abundance calculations suggest a large helium abundance (He/H ~0.29) in PM1-242. PM1-318 is a high-excitation (HeII4686/Hbeta ~1) planetary nebula with a ring-like inner shell containing two enhanced opposite regions, surrounded by a fainter round attached shell brighter in the light of [OIII]. PM1-333 is an extended planetary nebula with a high-excitation (HeII4686/Hbeta up to ~0.9) patchy circular main body containing two low-excitation knotty arcs. A low Ne([SII]) of ~450 cm-3 and Te([OIII]) of ~15000 K are derived for this nebula. Abundance calculations suggest that PM1-333 is a type I planetary nebula. The lack of a sharp shell morphology, low electron density, and high-excitation strongly suggest that PM1-333 is an evolved planetary nebula. PM1-333 also shows two low-ionization polar structures whose morphology and emission properties are reminiscent of collimated outflows. We compare PM1-333 with other evolved planetary nebulae with collimated outflows and find that outflows among evolved planetary nebulae exhibit a large variety of properties, in accordance with these observed in younger planetary nebula.Comment: Accepted in The Astronomical Journal, 23 pages, 6 figure

Antimicrobial Effect of Titanium Dioxide Nanoparticles

The widespread use of antibiotics has led to the emergence of multidrug-resistant bacterial strains, and therefore a current concern for food safety and human health. The interest for new antimicrobial substances has been focused toward metal oxide nanoparticles. Specifically, titanium dioxide (TiO2) has been considered as an attractive antimicrobial compound due to its photocatalytic nature and because it is a chemically stable, non-toxic, inexpensive, and Generally Recognized as Safe (GRAS) substance. Several studies have revealed this metal oxide demonstrates excellent antifungal and antibacterial properties against a broad range of both Gram-positive and Gram-negative bacteria. These properties were significantly improved by titanium dioxide nanoparticles (TiO2 NPs) synthesis. In this chapter, latest developments on routes of synthesis of TiO2 NPs and antimicrobial activity of these nanostructures are presented. Furthermore, TiO2 NPs favor the inactivation of microorganisms due to their strong oxidizing power by free radical generation, such as hydroxyl and superoxide anion radicals, showing reductions growth against several microorganisms, such as Escherichia coli and Staphylococcus aureus. Understanding the main mechanisms of antimicrobial action of these nanoparticles was the second main purpose of this chapter

In vivo potential of recombinant granulysin against human melanoma

9-kDa granulysin is a protein expressed into the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It has been shown to exert cytolysis on microbes and tumors. We showed previously that 9-kDa granulysin exerted cell death by apoptosis in vitro on hematological tumor cell lines and also on cells from B-cell chronic lymphocytic leukemia (B-CLL) patients. In addition, we have shown the anti-tumor efficiency of granulysin as a single agent in two in vivo models of human tumor development in athymic mice, the MDA-MB-231 mammary adenocarcinoma and the NCI-H929 multiple myeloma, without signs of overt secondary effects by itself. In this work, we have tested recombinant 9-kDa granulysin in an in vivo and especially aggressive model of melanoma development, xenografted UACC62 cells in athymic mice. Recombinant granulysin was administered once UACC62-derived tumors were detectable and it substantially retarded the in vivo development of this aggressive tumor. We could also detect apoptosis induction and increased NK cell infiltration inside granulysin-treated tumor tissues. These observations are especially interesting given the possibility of treating melanoma by intra-tumor injection

Self-assembled zinc blende GaN quantum dots grown

Zinc blende ~ZB! GaN quantum dots have been grown by plasma-assisted molecular-beam epitaxy on AlN buffer layers using 3C-SiC~001! substrates. The two- to three-dimensional growth mode transition is studied by following the evolution of the reflection high-energy electron diffraction pattern. ZB GaN island layers are further examined by atomic force microscopy and transmission electron microscopy, extracting a mean island height of 1.6 nm and a mean diameter of 13 nm at a density of 1.331011 cm22. Embedded ZB GaN quantum dots show strong ultraviolet photoluminescence without any thermal quenching up to room temperature.SFERERegion Rhône-AlpesConsejo Nacional de Ciencia y Tecnologí

Optical properties of cubic AlGaN

In this work we report optical characterization on several cubic c-AlGaN layers grown by MBE on SiC on Si pseudo-substrates, with different aluminum concentrations ranging from 0 to 70 %. Excitation power evolution of AlGaN photoluminescence (PL) spectra as well as reflectivity spectra allow to attribute PL peak to band gap recombination. PL energy dependence versus aluminum concentration is given. Reflectivity investigations are performed in the energy range between 1.5 eV and 4 eV on the samples. Theoretical calculations of multilayered structure reflectivity are fitted to experimental results, allowing an accurate determination of refractive index evolution versus Al concentration. From this analysis, qualitative information about interface roughness at AlGaN/SiC is also be derived.SFERERegion Rhône-AlpesConsejo Nacional de Ciencia y Tecnologí

DNA-based Self-Assembly of Chiral Plasmonic Nanostructures with Tailored Optical Response

Surface plasmon resonances generated in metallic nanostructures can be utilized to tailor electromagnetic fields. The precise spatial arrangement of such structures can result in surprising optical properties that are not found in any naturally occurring material. Here, the designed activity emerges from collective effects of singular components equipped with limited individual functionality. Top-down fabrication of plasmonic materials with a predesigned optical response in the visible range by conventional lithographic methods has remained challenging due to their limited resolution, the complexity of scaling, and the difficulty to extend these techniques to three-dimensional architectures. Molecular self-assembly provides an alternative route to create such materials which is not bound by the above limitations. We demonstrate how the DNA origami method can be used to produce plasmonic materials with a tailored optical response at visible wavelengths. Harnessing the assembly power of 3D DNA origami, we arranged metal nanoparticles with a spatial accuracy of 2 nm into nanoscale helices. The helical structures assemble in solution in a massively parallel fashion and with near quantitative yields. As a designed optical response, we generated giant circular dichroism and optical rotary dispersion in the visible range that originates from the collective plasmon-plasmon interactions within the nanohelices. We also show that the optical response can be tuned through the visible spectrum by changing the composition of the metal nanoparticles. The observed effects are independent of the direction of the incident light and can be switched by design between left- and right-handed orientation. Our work demonstrates the production of complex bulk materials from precisely designed nanoscopic assemblies and highlights the potential of DNA self-assembly for the fabrication of plasmonic nanostructures.Comment: 5 pages, 4 figure

A role for dystroglycan in the pathophysiology of acute leukemic cells.

AIMS: Previous reports have demonstrated that alterations or reduced expression of Dystroglycan (Dg) complex (αDg and βDg subunits) are related to progression and severity of neoplastic solid tissues. Therefore we determined the expression pattern and subcellular distribution of Dg complex in Acute Myeloid Leukemia (AML) primary blasts (M1, M2, and M3 phenotypes), as well as HL-60 and Kasumi-1 leukemia cell lines. Additionally, we evaluated the relative expression of the main enzymes controlling α-Dg glycosylation to ascertain the post-translational modifications in the leukemia cell phenotype. MAIN METHODS: Primary leukemia blasts and leukemia cell lines were processed by confocal analysis to determine the subcellular distribution of α-Dg, β-Dg, and phosphorylated β-Dg (Y892), to evaluate the expression pattern of the different Dg species we performed Western Blot (WB) assays, while the messenger RNA (mRNA) expression of enzymes involved in α-Dg glycosylation, such as POMGnT1, POMT1, POMT2, LARGE, FKTN, and FKRP, were evaluated by qualitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). Finally, in an attempt to ameliorate the leukemia cell phenotype, we transfected leukemia cells with a plasmid expressing the Dg complex. KEY FINDINGS: The Dg complex was altered in leukemia cells, including decreased mRNA, protein, and α-Dg glycosylated levels, mislocalization of β-Dg, and a diminution of mRNA expression of LARGE in patients leukemia blasts and in cell lines. Interestingly, the exogenous expression of Dg complex promoted filopodial formation, differentiation, and diminished proliferation, attenuating some HL-60 and Kasumi cells characteristics. SIGNIFICANCE: Dg complex integrity and balance are required for a proper hematopoietic cell function, in that its disruption might contribute to leukemia pathophysiology