Human germinal center B cells differ from naive and memory B cells by their aggregated MHC class II‐rich compartments lacking HLA‐DO

To generate memory B cells bearing high‐affinity antibodies, naive B cells first encounter antigen in the T cell‐rich areas of secondary lymphoid organs. There, they are activated by antigen‐specific T cells and become germinal center (GC) founder B cells. GC founders enter the GC to become centroblasts that proliferate and mutate their BCR. Centroblasts differentiate into centrocytes that undergo selection, which requires both the recognition/capture of antigen on follicular dendritic cells and the presentation of processed antigen to GC T cells. Because at each stage of differentiation B cells act as antigen‐presenting cells, we analyzed their content of HLA‐DR+‐rich compartments (MIIC), as well as their expression of HLA‐DM, which catalyzes peptide loading of class II molecules, and HLA‐DO, which interacts with HLA‐DM and focuses MHC class II peptide loading on antigens internalized by the BCR. Naive and memory B cells concentrate HLA‐DR, ‐DM and ‐DO into compartments dispersed under the cell surface, which are identified by their expression of lysosome‐associated membrane protein (Lamp)‐1 as late endosomes/lysosomes. GC founders and GC B cells express larger Lamp‐1+DR+ compartments that are concentrated in the juxta‐nuclear region. These compartments express lower levels of HLA‐DM and virtually no HLA‐DO. Upon induction of a GC founder phenotype through the prolonged (days) co‐ligation of BCR and CD40, the naive B cell's peripheral DR+DM+Lamp‐1+ compartments aggregate in a polar fashion close to the nucleus. Furthermore, HLA‐DO expression virtually disappears, whereas low levels of HLA‐DM remain co‐localized with HLA‐DR. Anti‐κ/λ antibodies, used as surrogate antigens, are promptly (minutes) endocytosed in naive, memory and GC B cells. Then, naive and memory B cells target the surrogate antigen to their peripheral HLA‐DO+ MIIC, while GC B cells target it to their HLA‐DO- MIIC aggregates. Taken together, our results show that human GC B cells differ from naive and memory B cells by their aggregated MIIC that lack HLA‐D

Epidemiological study of canine trypanosomosis in an urban area of Ivory Coast

Following confirmed cases of trypanosomosis in military working dogs, c cross-sectional study was undertaken to evaluate the source of infection and determine the prevalence of canine infection with Trypanosoma congolense in the urban focus of Abidjan, Ivory Coast. Blood from 123 dogs were collected and subjected to PCR using specific primers for Trypanosoma congolense "forest type". In addition, an entomological study was conducted in an urban area near the forest surronding the military camp. The observed prevalence was 30.1% end PCR positivity to Trypanosoma congolense was not significantly associated with sex or age of animals. This study demonstrates the high contamination rate of dogs in enzootic zones, the potential risk of introduction of the disease in free animal populations and the ability of Glossina palpalis to adopt to urban areas and to transmit trypanosomosis in such areas. The factors leading to a possible emergence of canine trypanosomiasis in enzootic zones need further investigations

Stars and gas in the very large interacting galaxy NGC 6872

The dynamical evolution of the large (> 100 kpc), barred spiral galaxy NGC 6872 and its small companion IC 4970 in the southern group Pavo is investigated. We present N-body simulations with stars and gas and 21 cm HI observations carried out with the Australia Telescope Compact Array of the large-scale distribution and kinematics of atomic gas. HI is detected toward the companion, corresponding to a gas mass of ca 1.3 10^9 Msun. NGC 6872 contains ca 1.4 10^{10} Msun of HI gas, distributed in an extended rotating disk. Massive concentrations of gas (10^9 Msun) are found at the tip of both tidal tails and towards the break seen in the optical northern arm near the companion. We detect no HI counterpart to the X-ray trail between NGC 6872 and NGC 6876, the dominant elliptical galaxy in the Pavo group located 8 arcmin to the southeast. At the sensitivity and the resolution of the observations, there is no sign in the overall HI distribution that NGC 6876 has affected the evolution of NGC 6872. There is no evidence of ram pressure stripping either. The X-ray trail could be due to gravitational focusing of the hot gas in the Pavo group behind NGC 6872 as the galaxy moves supersonically through the hot medium. The simulations of a gravitational interaction with a small nearby companion on a low-inclination prograde passage are able to reproduce most of the observed features of NGC 6872, including the general morphology of the galaxy, the inner bar, the extent of the tidal tails and the thinness of the southern tail.Comment: 12 pages, 11 figures. Accepted for publication in Astronomy & Astrophysics. The resolution of the figures has been greatly reduced. The paper with the original figures can found at http://www.oso.chalmers.se/~horellou/PAPERS/2006n6872.pd

Multifrequency Study of The Radio Galaxy NGC326

We present the results of a multi-frequency study of the inversion symmetric radio galaxy NGC326 based on Very Large Array observations at 1.4, 1.6, 4.8, 8.5 and 14.9 GHz. The morphological, spectral and polarization properties of this peculiar object are studied at different levels of spatial resolutions. The interpretation of the data will be discussed in forthcoming papers.Comment: 15 pages, 15 ps figures, accepted by A&

The Northern ROSAT All-Sky (NORAS) Galaxy Cluster Survey I: X-ray Properties of Clusters Detected as Extended X-ray Sources

In the construction of an X-ray selected sample of galaxy clusters for cosmological studies, we have assembled a sample of 495 X-ray sources found to show extended X-ray emission in the first processing of the ROSAT All-Sky Survey. The sample covers the celestial region with declination $\delta \ge 0\deg$ and galactic latitude $|b_{II}| \ge 20\deg$ and comprises sources with a count rate $\ge 0.06$ counts s$^{-1}$ and a source extent likelihood of 7. In an optical follow-up identification program we find 378 (76%) of these sources to be clusters of galaxies. ...Comment: 61 pages; ApJS in press; fixed bug in table file; also available at (better image quality) http://www.xray.mpe.mpg.de/theorie/NORAS

Controlling the dimensionality of low-Rm MHD turbulence experimentally

International audienc

The Mass Function of an X-Ray Flux-Limited Sample of Galaxy Clusters

A new X-ray selected and X-ray flux-limited galaxy cluster sample is presented. Based on the ROSAT All-Sky Survey the 63 brightest clusters with galactic latitude |bII| >= 20 deg and flux fx(0.1-2.4 keV) >= 2 * 10^{-11} ergs/s/cm^2 have been compiled. Gravitational masses have been determined utilizing intracluster gas density profiles, derived mainly from ROSAT PSPC pointed observations, and gas temperatures, as published mainly from ASCA observations, assuming hydrostatic equilibrium. This sample and an extended sample of 106 galaxy clusters is used to establish the X-ray luminosity--gravitational mass relation. From the complete sample the galaxy cluster mass function is determined and used to constrain the mean cosmic matter density and the amplitude of mass fluctuations. Comparison to Press--Schechter type model mass functions in the framework of Cold Dark Matter cosmological models and a Harrison--Zeldovich initial density fluctuation spectrum yields the constraints OmegaM = 0.12^{+0.06}_{-0.04} and sigma8 = 0.96^{+0.15}_{-0.12} (90% c.l.). Various possible systematic uncertainties are quantified. Adding all identified systematic uncertainties to the statistical uncertainty in a worst case fashion results in an upper limit OmegaM < 0.31. For comparison to previous results a relation sigma8 = 0.43 OmegaM^{-0.38} is derived. The mass function is integrated to show that the contribution of mass bound within virialized cluster regions to the total matter density is small, i.e., OmegaCluster = 0.012^{+0.003}_{-0.004} for cluster masses larger than 6.4^{+0.7}_{-0.6} * 10^{13} h_{50}^{-1} Msun.Comment: 35 pages; accepted for publication in The Astrophysical Journal; this and related papers, supplementary information, as well as electronic files of the tables given in this paper are available at http://www.astro.virginia.edu/~thr4f

Survey of Canine Dirofilaria immitis Infection in New Caledonia

Canine dirofilariosis is a frequent parasitic disease in New-Caledonia. A survey of canine heartworm (Dirofilaria immitis) infection among dogs from the cities of Tontouta, Nandaï and Nouméa, was performed in March 2009 using two antigen test kits; the microwell ELISA test: DiroCHE (Synbiotics Europe) and the Rapid Immuno Migration (RIM) test: WITNESS DIROFILARIA (Synbiotics Europe). Blood samples were collected from 64 dogs: 49 strays and 15 military working dogs. The military dogs received a permanent chemoprophylaxis (moxidectin). In 11 stray dogs, both tests were positive (22.4%). All the military dogs were negative, showing efficiency of chemoprophaxis. Results were discrepant in 6 dogs, negative with one test and doubtful with the other. Antigen heartworm test kits are available and reliable diagnostic tools. They are useful to evaluate the efficiency of chemoprophylaxis and to detect infected animals in order to treat them and to prevent the spreading of the disease

The Updated Zwicky Catalog (UZC)

The Zwicky Catalog of galaxies (ZC), with m_Zw<=15.5mag, has been the basis for the Center for Astrophysics (CfA) redshift surveys. To date, analyses of the ZC and redshift surveys based on it have relied on heterogeneous sets of galaxy coordinates and redshifts. Here we correct some of the inadequacies of previous catalogs by providing: (1) coordinates with <~2 arcsec errors for all of the Nuzc catalog galaxies, (2) homogeneously estimated redshifts for the majority (98%) of the data taken at the CfA (14,632 spectra), and (3) an estimate of the remaining "blunder" rate for both the CfA redshifts and for those compiled from the literature. For the reanalyzed CfA data we include a calibrated, uniformly determined error and an indication of the presence of emission lines in each spectrum. We provide redshifts for 7,257 galaxies in the CfA2 redshift survey not previously published; for another 5,625 CfA redshifts we list the remeasured or uniformly re-reduced value. Among our new measurements, Nmul are members of UZC "multiplets" associated with the original Zwicky catalog position in the coordinate range where the catalog is 98% complete. These multiplets provide new candidates for examination of tidal interactions among galaxies. All of the new redshifts correspond to UZC galaxies with properties recorded in the CfA redshift compilation known as ZCAT. About 1,000 of our new measurements were motivated either by inadequate signal-to-noise in the original spectrum or by an ambiguous identification of the galaxy associated with a ZCAT redshift. The redshift catalog we include here is ~96% complete to m_Zw<=15.5, and ~98% complete (12,925 galaxies out of a total of 13,150) for the RA(1950) ranges [20h--4h] and [8h--17h] and DEC(1950) range [-2.5d--50d]. (abridged)Comment: 34 pp, 7 figs, PASP 1999, 111, 43