272 research outputs found
Reorientation kinetics of superparamagnetic nanostructured rods
The attractive interactions between oppositely charged species (colloids,
macromolecules etc) dispersed in water are strong, and the direct mixing of
solutions containing such species generally yields to a precipitation, or to a
phase separation. We have recently developed means to control the
electrostatically-driven attractions between nanoparticles and polymers in
water, and at the same time to preserve the stability of the dispersions. We
give here an account of the formation of supracolloidal aggregates obtained by
co-assembly of 7 nm particles with copolymers. Nanostructured rods of length
comprised between 5 and 50 microns and diameter 500 nm were investigated. By
application of a magnetic field, the rods were found to reorient along with the
magnetic field lines. The kinetics of reorientation was investigated using step
changes of the magnetic field of amplitude 90 degrees. From the various results
obtained, among which an exponential decay of the tangent of the angle made
between the rod and the field, we concluded that the rods are
superparamagnetic.Comment: 12 pages - 452kB 7 - figures - 1 Table will be published in Journal
of Physics : Condensed Matte
Electrosteric enhanced stability of functional sub-10 nm cerium and iron oxide particles in cell culture medium
Applications of nanoparticles in biology require that the nanoparticles
remain stable in solutions containing high concentrations of proteins and
salts, as well as in cell culture media. In this work, we developed simple
protocols for the coating of sub-10 nm nanoparticles and evaluated the
colloidal stability of dispersions in various environments. Ligands (citric
acid), oligomers (phosphonate-terminated poly(ethylene oxide)) and polymers
(poly(acrylic acid)) were used as nanometer-thick adlayers for cerium (CeO2)
and iron (gamma-Fe2O3) oxide nanoparticles. The organic functionalities were
adsorbed on the particle surfaces via physical (electrostatic) forces.
Stability assays at high ionic strength and in cell culture media were
performed by static and dynamic light scattering. Among the three coating
examined, we found that only poly(acrylic acid) fully preserved the dispersion
stability on the long term (> weeks). The improved stability was explained by
the multi-point attachments of the chains onto the particle surface, and by the
adlayer-mediated electrosteric interactions. These results suggest that
anionically charged polymers represent an effective alternative to conventional
coating agents.Comment: 8 figures, 10 pages, 4 tables. to appear in Langmui
Delayed hepatic uptake of multi-phosphonic acid poly(ethylene glycol) coated iron oxide measured by real-time Magnetic Resonance Imaging
We report on the synthesis, characterization, stability and pharmacokinetics
of novel iron based contrast agents for magnetic resonance imaging (MRI).
Statistical copolymers combining multiple phosphonic acid groups and
poly(ethylene glycol) (PEG) were synthesized and used as coating agents for 10
nm iron oxide nanocrystals. In vitro, protein corona and stability assays show
that phosphonic acid PEG copolymers outperform all other coating types
examined, including low molecular weight anionic ligands and polymers. In vivo,
the particle pharmacokinetics is investigated by monitoring the MRI signal
intensity from mouse liver, spleen and arteries as a function of the time,
between one minute and seven days after injection. Iron oxide particles coated
with multi-phosphonic acid PEG polymers are shown to have a blood circulation
lifetime of 250 minutes, i.e. 10 to 50 times greater than that of recently
published PEGylated probes and benchmarks. The clearance from the liver takes
in average 2 to 3 days and is independent of the core size, coating and
particle stability. By comparing identical core particles with different
coatings, we are able to determine the optimum conditions for stealth MRI
probes.Comment: 19 pages 8 figures, RSC Advances, 201
Intracellular micro-rheology probed by micron-sized wires
In the last decade, rapid advances have been made in the field of
micro-rheology of cells and tissues. Given the complexity of living systems,
there is a need for the development of new types of nano- and micron-sized
probes, and in particular of probes with controlled interactions with the
surrounding medium. In the present paper, we evaluate the use of micron-sized
wires as potential probes of the mechanical properties of cells. The wire-based
micro-rheology technique is applied to living cells such as murine fibroblasts
and canine kidney epithelial cells. The mean-squared angular displacement
(MSAD) of wires associated to their rotational dynamics is obtained as a
function of the time using optical microscopy and image processing. It reveals
a Brownian-like diffusive regime where the MSA scale linearly with time and as
the inverse of the cube of the wire length. This scaling suggests that an
effective viscosity of the intracellular medium can be determined, and that in
the range 1 - 10 micrometers it does not depend on the length scale over which
it is measured.Comment: 13 page, 5 figures, accepted at Biomaterial
Linear and nonlinear rheology of wormlike micelles
Several surfactant molecules self-assemble in solution to form long,
cylindrical, flexible wormlike micelles. These micelles can be entangled with
each other leading to viscoelastic phases. The rheological properties of such
phases are very interesting and have been the subject of a large number of
experimental and theoretical studies in recent years. We shall report on our
recent work on the macrorheology, microrheology and nonlinear flow behaviour of
dilute aqueous solutions of a surfactant CTAT (Cetyltrimethylammonium
Tosilate). This system forms elongated micelles and exhibits strong
viscoelasticity at low concentrations ( 0.9 wt%) without the addition of
electrolytes. Microrheology measurements of have been done using
diffusing wave spectroscopy which will be compared with the conventional
frequency sweep measurements done using a cone and plate rheometer. The second
part of the paper deals with the nonlinear rheology where the measured shear
stress is a nonmonotonic function of the shear rate . In
stress-controlled experiments, the shear stress shows a plateau for
larger than some critical strain rate, similar to the earlier
reports on CPyCl/NaSal system. Cates et al have proposed that the plateau is a
signature of mechanical instability in the form of shear bands. We have carried
out extensive experiments under controlled strain rate conditions, to study the
time-dependence of shear stress. The measured time series of shear stress has
been analysed in terms of correlation integrals and Lyapunov exponents to show
unambiguously that the behaviour is typical of low dimensional dynamical
systems.Comment: 15 pages, 10 eps figure
Universal scattering behavior of co-assembled nanoparticle-polymer clusters
Water-soluble clusters made from 7 nm inorganic nanoparticles have been
investigated by small-angle neutron scattering. The internal structure factor
of the clusters was derived and exhibited a universal behavior as evidenced by
a correlation hole at intermediate wave-vectors. Reverse Monte-Carlo
calculations were performed to adjust the data and provided an accurate
description of the clusters in terms of interparticle distance and volume
fraction. Additional parameters influencing the microstructure were also
investigated, including the nature and thickness of the nanoparticle adlayer.Comment: 5 pages, 4 figures, paper published in Physical Review
Electrostatic co-assembly of iron oxide nanoparticles and polymers : towards the generation of highly persistent superparamagnetic nanorods
A paradigm proposed recently by Boal et al. (A.K. Boal et al., Nature 404,
746-748, 2000) deals with the possibility to use inorganic nanoparticles as
building blocks for the design and fabrication of colloidal and supracolloidal
assemblies. It is anticipated that these constructs could be made of different
shapes, patterns and functionalities and could constitute the components of
future nanodevices including sensors, actuators or nanocircuits. Here we report
a protocol that allowed us to fabricate such nanoparticle aggregates. The
building blocks of the constructs were anionically coated iron oxide
nanocrytals (superparamagnetic, size 7 nm) and cationic-neutral block
copolymers. We have shown that the electrostatic interactions between charged
species can be controlled by tuning the ionic strength of the dispersion. Under
appropriate conditions, the control of electrostatics resulted in the
elaboration of spherical or elongated aggregates at the micrometer length
scale. The elongated aggregates were found to be rod-like, with diameters of a
few hundred nanometers and lengths between 1 and 50 micrometers. In addition to
their remarkable stiffness, the nanostructured rods were found to reorient
along with an externally applied magnetic field, in agreement with the laws of
superparamagnetism.Comment: 6 pages, 5 figures, appeared in Advanced materials in September 2008,
reference
Loss of solutions in shear banding fluids in shear banding fluids driven by second normal stress differences
Edge fracture occurs frequently in non-Newtonian fluids. A similar
instability has often been reported at the free surface of fluids undergoing
shear banding, and leads to expulsion of the sample. In this paper the
distortion of the free surface of such a shear banding fluid is calculated by
balancing the surface tension against the second normal stresses induced in the
two shear bands, and simultaneously requiring a continuous and smooth meniscus.
We show that wormlike micelles typically retain meniscus integrity when shear
banding, but in some cases can lose integrity for a range of average applied
shear rates during which one expects shear banding. This meniscus fracture
would lead to ejection of the sample as the shear banding region is swept
through. We further show that entangled polymer solutions are expected to
display a propensity for fracture, because of their much larger second normal
stresses. These calculations are consistent with available data in the
literature. We also estimate the meniscus distortion of a three band
configuration, as has been observed in some wormlike micellar solutions in a
cone and plate geometry.Comment: 23 pages, to be published in Journal of Rheolog
Dynamic Response of Block Copolymer Wormlike Micelles to Shear Flow
The linear and non-linear dynamic response to an oscillatory shear flow of
giant wormlike micelles consisting of Pb-Peo block copolymers is studied by
means of Fourier transform rheology. Experiments are performed in the vicinity
of the isotropic-nematic phase transition concentration, where the location of
isotropic-nematic phase transition lines is determined independently. Strong
shear-thinning behaviour is observed due to critical slowing down of
orientational diffusion as a result of the vicinity of the isotropic- nematic
spinodal. This severe shear-thinning behaviour is shown to result in gradient
shear banding. Time-resolved Small angle neutron scattering experiments are
used to obtain insight in the microscopic phenomena that underly the observed
rheological response. An equation of motion for the order-parameter tensor and
an expression of the stress tensor in terms of the order-parameter tensor are
used to interpret the experimental data, both in the linear and non-linear
regime. Scaling of the dynamic behaviour of the orientational order parameter
and the stress is found when critical slowing down due to the vicinity of the
isotropic-nematic spinodal is accounted for.Comment: Accepted by J. Phys.: Condens. Matter, CODEF II Special Issue. 20
pages, 9 figure
Dynamic Response of Block Copolymer Wormlike Micelles to Shear Flow
The linear and non-linear dynamic response to an oscillatory shear flow of
giant wormlike micelles consisting of Pb-Peo block copolymers is studied by
means of Fourier transform rheology. Experiments are performed in the vicinity
of the isotropic-nematic phase transition concentration, where the location of
isotropic-nematic phase transition lines is determined independently. Strong
shear-thinning behaviour is observed due to critical slowing down of
orientational diffusion as a result of the vicinity of the isotropic- nematic
spinodal. This severe shear-thinning behaviour is shown to result in gradient
shear banding. Time-resolved Small angle neutron scattering experiments are
used to obtain insight in the microscopic phenomena that underly the observed
rheological response. An equation of motion for the order-parameter tensor and
an expression of the stress tensor in terms of the order-parameter tensor are
used to interpret the experimental data, both in the linear and non-linear
regime. Scaling of the dynamic behaviour of the orientational order parameter
and the stress is found when critical slowing down due to the vicinity of the
isotropic-nematic spinodal is accounted for.Comment: Accepted by J. Phys.: Condens. Matter, CODEF II Special Issue. 20
pages, 9 figure
- …