Performance and cost efficiency of KRAS mutation testing for metastatic colorectal cancer in routine diagnosis: the MOKAECM study, a nationwide experience.

International audiencePURPOSE: Rapid advances in the understanding of cancer biology have transformed drug development thus leading to the approval of targeted therapies and to the development of molecular tests to select patients that will respond to treatments. KRAS status has emerged as a negative predictor of clinical benefit from anti-EGFR antibodies in colorectal cancer, and anti-EGFR antibodies use was limited to KRAS wild type tumors. In order to ensure wide access to tumor molecular profiling, the French National Cancer Institute (INCa) has set up a national network of 28 regional molecular genetics centers. Concurrently, a nationwide external quality assessment for KRAS testing (MOKAECM) was granted to analyze reproducibility and costs. METHODS: 96 cell-line DNAs and 24 DNA samples from paraffin embedded tumor tissues were sent to 40 French laboratories. A total of 5448 KRAS results were collected and analyzed and a micro-costing study was performed on sites for 5 common methods by an independent team of health economists. RESULTS: This work provided a baseline picture of the accuracy and reliability of KRAS analysis in routine testing conditions at a nationwide level. Inter-laboratory Kappa values were >0.8 for KRAS results despite differences detection methods and the use of in-house technologies. Specificity was excellent with only one false positive in 1128 FFPE data, and sensitivity was higher for targeted techniques as compared to Sanger sequencing based methods that were dependent upon local expertise. Estimated reagent costs per patient ranged from €5.5 to €19.0. CONCLUSION: The INCa has set-up a network of public laboratories dedicated to molecular oncology tests. Our results showed almost perfect agreements in KRAS testing at a nationwide level despite different testing methods ensuring a cost-effective equal access to personalized colorectal cancer treatment

Interferences of Peltier thermal waves produced in ohmic contacts upon integrated circuits

We have experimentally detected a Peltier effect produced by the current flow in ohmic contacts upon integrated circuits. These contacts are small size heat sources which are very useful for investigating integrated circuits. We have characterised their thermal properties with a high resolution interferometric laser probe by measuring absolute thermal expansions. Subpicometric resolution has allowed original imaging of semiconductor components : we have mapped the thermal waves created at ohmic contacts under normal operating conditions

Equipement d'une centrale de technologie de microelectronique hybride a l'Universite de Bordeaux 1. Premiere tranche: couches epaisses

SIGLECNRS-CDST / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Melodic contour and mid-level global features applied to the analysis of flamenco cantes

This work focuses on the topic of melodic characterization and similarity in a specific musical repertoire: a cappella flamenco singing, more specifically in debla and martinete styles. We propose the combination of manual and automatic description. First, we use a state-of-the-art automatic transcription method to account for general melodic similarity from music recordings. Second, we define a specific set of representative mid-level melodic features, which are manually labelled by flamenco experts. Both approaches are then contrasted and combined into a global similarity measure. This similarity measure is assessed by inspecting the clusters obtained through phylogenetic algorithms and by relating similarity to categorization in terms of style. Finally, we discuss the advantage of combining automatic and expert annotations as well as the need to include repertoire-specific descriptions for meaningful melodic characterization in traditional music collections.This article has been funded by the Andalusian Government under a Proyecto de Excelencia research project with reference number P12-TIC-1362