Comparison of Gastrocnemius Shear Wave Elastography Stiffness Over 5 Different Zones

A few studies report that shear wave elastography (SWE) is a reliable method of measuring gastrocnemius muscle stiffness. To date there are no studies assessing variance in stiffness measures at different locations on the muscle. This led us to ask whether the spot at which gastrocnemius stiffness is measured matters. PURPOSE: To determine if measurement site on the medial and lateral gastrocnemius affects SWE stiffness values. METHODS: Twelve subjects (3 men, 9 women) completed this study (Mean age is 23.0 (SD = 1.0) for men and 21.7 (SD = 2.0) for women (t p = 0.3035). Mean height (cm) is 179.5 (SD = 2.9) for men and 167.1 (SD = 7.8) for women (t p = 0.0256). Mean weight (kg) is 74.9 (SD =10.4) for men and 62.5 (SD = 9.7) for women (t p = 0.0892). Subjects wore shorts and lay prone on a treatment table with their bare feet hanging off the edge of the table. Ultrasound images to confirm borders of the medial and lateral gastrocnemius were confirmed and marked. SWE of both the Medial head (MG) and lateral head (LG) were taken with each head area divided into 4 zones (1=superior medial, 2=superior lateral, 3=inferior medial, 4= inferior lateral). A fifth zone was collected at the center of the muscle at 70% of the length of the lateral malleolus to lateral femoral epicondyle. All 5 points were assessed for SWE in both a relaxed and neutral (900) ankle joint position. Three separate elastogram frames were used to calculate muscle stiffness using both the velocity (m/s) and young’s modulus model (kPa) utilizing an overall area analysis for each. RESULTS: MG velocity and kPa values were significantly greater than LG in the relaxed and neutral positions (pCONCLUSION: MG stiffness is greater than LG regardless of position while intrinsic stiffness of MG increases more relative to LG when placed in neutral. Measurement of stiffness is best represented at the center of the muscle belly in the neutral position, but zones 2,3 and 5 produce similar stiffness values in the relaxed position

Acute Stretching Effect on Hamstring Muscle Stiffness using Elastography

Having first been introduced in the 1990s, shear wave elastography (SWE) has more recently been used to investigate intrinsic muscle stiffness. While studies have shown SWE to be an effective way of determining muscle stiffness, few have been conducted to measure the effects of stretching on the hamstring muscles. PURPOSE: to determine if there is a measurable difference of hamstring muscle stiffness with acute stretching using ultrasound elastography. METHODS: 16 subjects participated in this study (11 men and 5 women). Mean age 23.4 ± 2.6yrs for men and 21.2 ±1.5 for women. Mean height (cm) is 180.3± 4.7 for men and 172.7±2.5 for women. Mean weight (kg) is 76.8±9.7 for men and 70.0±14.9 for women. Participants lay supine and positioned to 90° hip and 90° knee flexion. Each participant came in for two sessions that consisted of (1) static stretching and (2) PNF stretching protocols. Maximum ROM of the knee joint, force, and muscle stiffness of the biceps femoris (BF), semimembranosus (SM) and semitendinosus (ST) were recorded both before and 1 minute after stretching. RESULTS: After accounting for age, weight and height, there was no significant difference between the use of static and PNF stretching techniques on either velocity (p= 0.4805) or kPa (p=0.5423) stiffness values. Both static and PNF stretching resulted in significant reductions in both velocity and kPa stiffness (pCONCLUSION: Stiffness as measured using SWE decreases following an acute stretching session for both static and PNF stretching techniques. This suggests alterations to intrinsic muscle characteristics beyond just “stretch tolerance” as suggested in previous literature. Changes in both ROM and force also support this claim. Further research on retention of decreased stiffness are needed as acute stretching has previously been shown to be temporary. Longer term stretching studies to determine if intrinsic muscle stiffness changes models plastic deformation are also needed and may help better elucidate duration and stretch technique differences

The stability of the fractional quantum Hall effect in topological insulators

With the recent observation of graphene-like Landau levels at the surface of topological insulators, the possibility of fractional quantum Hall effect, which is a fundamental signature of strong correlations, has become of interest. Some experiments have reported intra-Landau level structure that is suggestive of fractional quantum Hall effect. This paper discusses the feasibility of fractional quantum Hall effect from a theoretical perspective, and argues that while this effect should occur, ideally, in the $n=0$ and $|n|=1$ Landau levels, it is ruled out in higher $|n|$ Landau levels. Unlike graphene, the fractional quantum Hall effect in topological insulators is predicted to show an interesting asymmetry between $n=1$ and $n=-1$ Landau levels due to spin-orbit coupling.Comment: 8 pages, 2 figure

The Sex Specific Effect of Alcohol Consumption on Circulating Levels of CTRP3

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The goal of this project was to establish the effect of alcohol consumption on the circulating levels of the adipose tissue derived protein C1q TNF Related Protein 3 (CTRP3). Adipose tissue secretes several adipokines, such as adiponectin and leptin, which exert a multitude of biological effects important for human health. However, adipose tissue is extremely sensitive to alcohol consumption, leading not only to disrupted fat storage, but also to disruptions in adipokine production. Changes to adipokine secretion could have widespread biological effects and potentially contribute to alcohol-induced ailments, such as alcoholic fatty liver disease (ALD). CTRP3 has been previously demonstrated to attenuate fatty liver disease, and suppression of CTRP3 with alcohol consumption could contribute to development of and progression to alcoholic fatty liver disease. To examine the effect of ethanol consumption on circulating adipokine levels, male and female mice were fed an ethanol containing diet (Lieber- DeCarli 5% (v/v) ethanol diet) for 10-days followed by a single gavage of 5 g/kg ethanol (the NIAAA model), or for 6-weeks with no binge added (chronic model). In female mice, adiponectin levels increased ~2-fold in both models of ethanol feeding, but in male mice increased adiponectin levels were only observed after chronic ethanol feeding. On the other hand, in female mice, circulating CTRP3 levels decreased by ~75% and ~50% in the NIAAA and chronic model, respectively, with no changes observed in the male mice in either feeding model. Leptin levels were unchanged with ethanol feeding regardless of model or sex of mice. Lastly, chronic ethanol feeding led to a significant increase in mortality (~50%) in female mice, with no difference in relative ethanol consumption. These findings indicate that ethanol consumption can dysregulate adipokine secretion, but that the effects vary by sex of animal, method of ethanol consumption, and adipokine examined. These findings also indicate that female mice are more sensitive to the chronic effects of ethanol than male mice. Notably, this is the first study to document the effects of ethanol consumption on the circulating levels of CTRP3. Understanding the impact of excessive alcohol consumption on adipokine production and secretion could identify novel mechanisms of alcohol-induced human disease. However, the mechanism responsible for the increased sensitivity remains elusive

In children, the microbiota of the nasopharynx and bronchoalveolar lavage fluid are both similar and different

RATIONALE: Sputum and bronchoalveolar lavage fluid (BALF) are often obtained to elucidate the lower airway microbiota in adults. Acquiring sputum samples from children is difficult and obtaining samples via bronchoscopy in children proves challenging due to the need for anesthesia and specialized procedural expertise; therefore nasopharyngeal (NP) swabs are often used as surrogates when investigating the pediatric airway microbiota. In adults, the airway microbiota differs significantly between NP and BALF samples however, minimal data exist in children. OBJECTIVES: To compare NP and BALF samples in children undergoing clinically indicated bronchoscopy. METHODS: NP and BALF samples were collected during clinically indicated bronchoscopy. Bacterial DNA was extracted from 72 samples (36 NP/BALF pairs); the bacterial V1-V3 region of the 16S rRNA gene was amplified and sequenced on the Illumina Miseq platform. Analysis was performed using mothur software. RESULTS: Compared to NP samples, BALF had increased richness and diversity. Similarity between paired NP and BALF (intra-subject) samples was greater than inter-subject samples (P = 0.0006). NP samples contained more Actinobacteria (2.2% vs 21%; adjusted P = 1.4 × 10-6 ), while BALF contained more Bacteroidetes (29.5% vs 3.2%; adjusted P = 1.2 × 10-9 ). At the genus level several differences existed, however Streptococcus abundance was similar in both sample types (NP 37.3% vs BAL 36.1%; adjusted P = 0.8). CONCLUSION: Our results provide evidence that NP samples can be used to distinguish differences between children, but the relative abundance of organisms may differ between the nasopharynx and lower airway in pediatric patients. Studies utilizing NP samples as surrogates for the lower airway should be interpreted with caution

Warm Absorbers and Outflows in the Seyfert-1 Galaxy NGC 4051

We present both phenomenological and more physical photoionization models of the Chandra HETG spectra of the Seyfert-1 AGN NGC 4051. We detect 40 absorption and emission lines, encompassing highly ionized charge states from O, Ne, Mg, Si, S and the Fe L-shell and K-shell. Two independent photoionization packages, XSTAR and Cloudy, were both used to self-consistently model the continuum and line spectra. These fits detected three absorbing regions in this system with densities ranging from 10^{10} to 10^{11} cm^{-3}. In particular, our XSTAR models require three components that have ionization parameters of log \xi = 4.5, 3.3, & 1.0, and are located within the BLR at 70, 300, and 13,000 R_g, respectively, assuming a constant wind density. Larger radii are inferred for density profiles which decline with radius. The Cloudy models give a similar set of parameters with ionization parameters of log \xi = 5.0, 3.6, & 2.2 located at 40, 200, and 3,300 R_g. We demonstrate that these regions are out-flowing from the system, and carry a small fraction of material out of the system relative to the implied mass accretion rate. The data suggest that magnetic fields may be an important driving mechanism.Comment: 21 pages, 11 Figures, Accepted to Ap

A Jet Model for the Broadband Spectrum of the Seyfert-1 Galaxy NGC 4051

Recent radio VLBI observations of the ~parsec-scale nuclear region of the narrow line Seyfert 1 galaxy NGC 4051 hint toward the presence of outflowing plasma. From available literature we have collected high-quality, high-resolution broadband spectral energy distribution data of the nuclear region of NGC 4051 spanning from radio through X-rays, to test whether the broadband SED can be explained within the framework of a relativistically outflowing jet model. We show that once the contribution from the host galaxy is taken into account, the broadband emission from the active galactic nucleus of NGC 4051 can be well described by the jet model. Contributions from dust and ongoing star-formation in the nuclear region tend to dominate the IR emission even at the highest resolutions. In the framework of the jet model, the correlated high variability of the extreme ultraviolet and X-rays compared to other wavelengths suggests that the emission at these wavelengths is optically thin synchrotron originating in the particle acceleration site(s) in the jet very close (few $r_g=GM_{BH}/c^2$) to the central supermassive black hole of mass M_{BH}. Our conclusions support the hypothesis that narrow line Seyfert 1 galaxies (which NGC 4051 is a member of) harbor a "jetted" outflow with properties similar to what has already been seen in low-luminosity AGNs and stellar mass black holes in hard X-ray state.Comment: Accepted for publication in ApJ. 24 pages, 2 figures, 2 tables (in aastex preprint format

An NF-κB - EphrinA5-Dependent Communication between NG2+ Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates

SummarySkeletal muscle growth immediately following birth is critical for proper body posture and locomotion. However, compared with embryogenesis and adulthood, the processes regulating the maturation of neonatal muscles is considerably less clear. Studies in the 1960s predicted that neonatal muscle growth results from nuclear accretion of myoblasts preferentially at the tips of myofibers. Remarkably, little information has been added since then to resolve how myoblasts migrate to the ends of fibers. Here, we provide insight into this process by revealing a unique NF-κB-dependent communication between NG2+ interstitial cells and myoblasts. NF-κB in NG2+ cells promotes myoblast migration to the tips of myofibers through cell-cell contact. This occurs through expression of ephrinA5 from NG2+ cells, which we further deduce is an NF-κB target gene. Together, these results suggest that NF-κB plays an important role in the development of newborn muscles to ensure proper myoblast migration for fiber growth

Mapping our Universe in 3D with MITEoR

Mapping our universe in 3D by imaging the redshifted 21 cm line from neutral hydrogen has the potential to overtake the cosmic microwave background as our most powerful cosmological probe, because it can map a much larger volume of our Universe, shedding new light on the epoch of reionization, inflation, dark matter, dark energy, and neutrino masses. We report on MITEoR, a pathfinder low-frequency radio interferometer whose goal is to test technologies that greatly reduce the cost of such 3D mapping for a given sensitivity. MITEoR accomplishes this by using massive baseline redundancy both to enable automated precision calibration and to cut the correlator cost scaling from N^2 to NlogN, where N is the number of antennas. The success of MITEoR with its 64 dual-polarization elements bodes well for the more ambitious HERA project, which would incorporate many identical or similar technologies using an order of magnitude more antennas, each with dramatically larger collecting area.Comment: To be published in proceedings of 2013 IEEE International Symposium on Phased Array Systems & Technolog