School abductions in Chibok and Zamfara, Nigeria : the nexus between gender, terror and official responses

School abductions in Nigeria have received global attention since 2014. Although several studies exist on gender-based terrorism in Nigeria, none has sought to understand the relationship between gender, gender constructions, terror directed towards women and girls, and official responses. This thesis, therefore, addresses gender-based terrorism in Nigeria by focusing on responses to terror on women and the girl-child. Specifically, it analyses how gender and gender constructions influenced extremist groups in Nigeria to perpetrate violence against women and girls and what responses this generated from officials nationally and internationally. The research used both primary and secondary data. The primary data was collected through semi-structured interviews with academics, journalists, officials in Nigeria, and national and international organizations. The secondary data was sourced from academic publications, video material, reports, and other information released by non-governmental organizations, the media, and state institutions in Nigeria. The findings indicate that the socio-cultural fabric of the Nigerian society influenced the reasons for and rationale behind abductions of women and girls in the country. The study found that the socio-cultural fabric of the Nigerian society had created a sense of value for women and girls, which made extremist organizations in the country regard them as, firstly, a vital economic tool for bargaining and wealth making and, secondly, as a source of achieving group cohesion and loyalty among the extremists. The research also found that official responses to the cases under study were influenced by many factors, including gender, education, human rights abuses and forced separation from families; they also reflected concern with the rise of the Islamic State (IS) coupled with the objectives of Boko Haram. Moreover, the construction of gender and gender relations was connected to terror directed towards women and girls, and to the official responses to terror, through the unequal social and power relation, and the sense of value conditioned by the construction of gender, which created an avenue for extremists to exploit women and girls for their strategic advantage. Therefore, the study argues that fostering societal transformation, promotion of gender-sensitive prevention of violent extremism, and the enactment of comprehensive legislation are key factors in challenging gender-based violence, including terror. Overall, the thesis contributes to the growing literature on gender-based terrorism and the growing number of studies in international relations that focus on gender.M-I

Daily activity rhythm of the African stingless bee Hypotrigona gribodoi (Hymenoptera: Meliponini) in the dry season, with notes on nest structure and colony composition

We studied 18 colonies of Hypotrigona gribodoi (Magretti) nesting in the tubing of a disused horticultural sprinkler system during the late dry season in Ghana, West Africa.  Flight activity began around dawn (about 06:00), rising to a peak in the half-hour starting 07:30 and gradually declining to end around dusk (about 18:00).  Bees returning with pollen showed a more extended peak from 07:30 to 09:00.  Dissection of 18 nests showed a mean inner volume of 466.5 ml, while 17 active colonies had a mean number of 851 adults.  Colony size is positively correlated with nest size.  It is likewise positively correlated with the fraction of fully mature adult females. Several nests had branching entrance tubes with an angle of roughly 30⁰ between branches.  In each of three such nests studied, there was a marked division in the use of the two branches, such that one served mainly as an exit and the other as an entrance

Spatiotemporal co-existence of two Mycobacterium ulcerans clonal complexes in the Offin River Valley of Ghana

In recent years, comparative genome sequence analysis of African Mycobacterium ulcerans strains isolated from Buruli ulcer (BU) lesion specimen has revealed a very limited genetic diversity of closely related isolates and a striking association between genotype and geographical origin of the patients. Here, we compared whole genome sequences of five M. ulcerans strains isolated in 2004 or 2013 from BU lesions of four residents of the Offin river valley with 48 strains isolated between 2002 and 2005 from BU lesions of individuals residing in the Densu river valley of Ghana. While all M. ulcerans isolates from the Densu river valley belonged to the same clonal complex, members of two distinct clonal complexes were found in the Offin river valley over space and time. The Offin strains were closely related to genotypes from either the Densu region or from the Asante Akim North district of Ghana. These results point towards an occasional involvement of a mobile reservoir in the transmission of M. ulcerans, enabling the spread of bacteria across different regions

Development of Composite Material for Wind Turbine Blades

Rapid increases in the prices of conventional turbine blade making materials as well as future sustainability issues is attracting research interest in the development of polymer composites for turbine blade applications. This study examines the suitability of using a novel bamboo fibre and recycled plastics composite developed for wind turbine blade application. Bamboo fibre was extracted from raw bamboo by chemical and mechanical processes. Recycled High Density Polyethylene (HDPE) was collected, cut into pieces, cleaned and combined with extracted bamboo fibre to form the composite. The percentage ratio of fibre content in the ten specimens ranged from 2.5% to 25%. A series of mechanical tests were conducted on the specimens, including tensile test, impact test, water absorption test and sun radiation test. Results established that as the percentage of bamboo fibre in the specimen increased, the tensile strength and impact energy also increased. Water absorption and sun radiation tests conducted on specimen X revealed no meaningful impact its mechanical properties. Hence, the most significant deduction from this study is that the specimen with 25% bamboo fibre and 75% HDPE matrix possesses the quality that qualifies it to be used as a material for wind turbine blade fabrication

Relevance of genomic diversity of Mycobacterium tuberculosis complex in Africa

BACKGROUND: The diversity in the lineages of Mycobacterium tuberculosis complex (MTBC) was initially considered insignificant. However, comparative genomics analysis of MTBC have found genomic variation among the genotypes with potential phenotypic implications. OBJECTIVE: Therefore, this viewpoint seeks to discuss the impact of the identified genotypic diversity on the physiology of MTBC and the potential implications on TB control. RESULTS: Studies conducted in West Africa and other parts of Africa have unravelled the implications of the genomic diversity on phenotypes such as disease outcome, transmission dynamics and host immune response. The understanding of the phenotypic diversity among the different lineages of MTBC may be an important key to the fight against TB. CONCLUSION: The relevance of these differences has been observed in the design of new control tools such as diagnostics and anti-TB drugs/vaccines. This only points to the fact that the diversity in MTBC cannot be ignored in future studies especially clinical trials for new vaccines and new anti-TB drugs

Adherence to standard nursing protocols on nasogastric tube feeding in a secondary referral hospital in Ghana:comparing self-ratings by professional and auxiliary nurses

BackgroundData on nurses’ adherence to standard protocol on nasogastric (NG) tube feeding remain scanty in Ghana even though patients in critical medical conditions are routinely managed using this procedure. This study explored self-rated adherence to standard protocols on NG tube feeding among professional and auxiliary nurses and the perceived barriers impeding compliance to these standard protocols.MethodsThis is a descriptive analytical cross-sectional study among professional (n = 89) and auxiliary (n = 24) nurses in a major referral hospital in one of the ten administrative regions in Ghana. Four-point Likert scale was used to ascertain the level of adherence to standard guidelines on nasogastric tube, ranging from 4 “Very large extent” to 1 “Very little extent”. Wilcoxon Mann-Whitney test and univariate ordered logistic regression tests (proportional odds models) were performed to determine the odds of higher self-ratings among professional and auxiliary nurses.ResultsOverall, the odds of higher self-ratings on adherence to standard nursing protocols on NG tube feeding was higher among auxiliary nurses than professional nurses (OR = 2.76, p = 0.031) after adjusting for age, gender, education and years of work experience. Key barriers to adherence to standard protocols on NG tube feeding were: limited opportunities for in-service trainings and insufficiency of NG tube feeding protocols on the wards.ConclusionThere is the need for more routine in-service trainings for nursing staff to update their knowledge on NG tube feeding. Hospital management should also make current nursing protocols available to nurses to guide their practice alongside routine onsite supervision of nurses

Whole genome sequencing and spatial analysis identifies recent tuberculosis transmission hotspots in Ghana

Whole genome sequencing (WGS) is progressively being used to investigate the transmission dynamics of; Mycobacterium tuberculosis; complex (MTBC). We used WGS analysis to resolve traditional genotype clusters and explored the spatial distribution of confirmed recent transmission clusters. Bacterial genomes from a total of 452 MTBC isolates belonging to large traditional clusters from a population-based study spanning July 2012 and December 2015 were obtained through short read next-generation sequencing using the illumina HiSeq2500 platform. We performed clustering and spatial analysis using specified R packages and ArcGIS. Of the 452 traditional genotype clustered genomes, 314 (69.5%) were confirmed clusters with a median cluster size of 7.5 genomes and an interquartile range of 4-12. Recent tuberculosis (TB) transmission was estimated as 24.7%. We confirmed the wide spread of a Cameroon sub-lineage clone with a cluster size of 78 genomes predominantly from the Ablekuma sub-district of Accra metropolis. More importantly, we identified a recent transmission cluster associated with isoniazid resistance belonging to the Ghana sub-lineage of lineage 4. WGS was useful in detecting unsuspected outbreaks; hence, we recommend its use not only as a research tool but as a surveillance tool to aid in providing the necessary guided steps to track, monitor, and control TB

Second-line anti-tuberculosis drug resistance testing in Ghana identifies the first extensively drug-resistant tuberculosis case

Background: Drug resistance surveillance is crucial for tuberculosis (TB) control. Therefore, our goal was to determine the prevalence of second-line anti-TB drug resistance among diverse primary drug-resistant Mycobacterium tuberculosis complex (MTBC) isolates in Ghana. Materials and methods: One hundred and seventeen MTBC isolates with varying first-line drug resistance were analyzed. Additional resistance to second-line anti-TB drugs (streptomycin [STR], amikacin [AMK] and moxifloxacin [MOX]) was profiled using the Etest and GenoType MTBDRsl version 2.0. Genes associated with resistance to AMK and MOX (gyrA, gyrB, eis, rrs, tap, whiB7 and tlyA) were then analyzed for mutation. Results: Thirty-seven (31.9%) isolates had minimum inhibitory concentration (MIC) values ≥2 µg/mL against STR while 12 (10.3%) isolates had MIC values ≥1 µg/mL for AMK. Only one multidrug-resistant (MDR) isolate (Isolate ID: TB/Nm 919) had an MIC value of ≥0.125 µg/mL for MOX (MIC = 3 µg/mL). This isolate also had the highest MIC value for AMK (MIC = 16 µg/mL) and was confirmed as resistant to AMK and MOX by the line probe assay GenoType MTBDRsl version 2.0. Mutations associated with the resistance were: gyrA (G88C) and rrs (A514C and A1401G). Conclusion: Our findings suggest the need to include routine second-line anti-TB drug susceptibility testing of MDR/rifampicin-resistant isolates in our diagnostic algorithm

Molecular epidemiology and whole genome sequencing analysis of clinical Mycobacterium bovis from Ghana.

BACKGROUND: Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a re-emerging problem in both livestock and humans. The association of some M. bovis strains with hyper-virulence, MDR-TB and disseminated disease makes it imperative to understand the biology of the pathogen. METHODS: Mycobacterium bovis (15) among 1755 M. tuberculosis complex (MTBC) isolated between 2012 and 2014 were characterized and analyzed for associated patient demography and other risk factors. Five of the M. bovis isolates were whole-genome sequenced and comparatively analyzed against a global collection of published M. bovis genomes. RESULTS: Mycobacterium bovis was isolated from 3/560(0.5%) females and 12/1195(1.0%) males with pulmonary TB. The average age of M. bovis infected cases was 46.8 years (7-72years). TB patients from the Northern region of Ghana (1.9%;4/212) had a higher rate of infection with M. bovis (OR = 2.7,p = 0.0968) compared to those from the Greater Accra region (0.7%;11/1543). Among TB patients with available HIV status, the odds of isolating M. bovis from HIV patients (2/119) was 3.3 higher relative to non-HIV patients (4/774). Direct contact with livestock or their unpasteurized products was significantly associated with bTB (p<0.0001, OR = 124.4,95% CI = 30.1-508.3). Two (13.3%) of the M. bovis isolates were INH resistant due to the S315T mutation in katG whereas one (6.7%) was RIF resistant with Q432P and I1491S mutations in rpoB. M. bovis from Ghana resolved as mono-phyletic branch among mostly M. bovis from Africa irrespective of the host and were closest to the root of the global M. bovis phylogeny. M. bovis-specific amino acid mutations were detected among MTBC core genes such as mce1A, mmpL1, pks6, phoT, pstB, glgP and Rv2955c. Additional mutations P6T in chaA, G187E in mgtC, T35A in Rv1979c, S387A in narK1, L400F in fas and A563T in eccA1 were restricted to the 5 clinical M. bovis from Ghana. CONCLUSION: Our data indicate potential zoonotic transmission of bTB in Ghana and hence calls for intensified public education on bTB, especially among risk groups