Cyclin B–Cdk1 activates its own pump to get into the nucleus

The transition to mitosis requires extensive nuclear and cytoplasmic rearrangements that must be spatially and temporally coordinated. In this issue, Gavet and Pines (2010a. J. Cell Biol. doi:10.1083/jcb.200909144) report on a simple yet elegant mechanism as to how this is achieved. By monitoring the activity of cyclin B–Cdk1 in real time, the authors show that concomitant with its activation in the cytoplasm, the kinase complex is rapidly imported into the nucleus by modifying the activity of the nucleocytoplasmic transport machinery. Thus, cyclin B–Cdk1 activates its own pump to get into the nucleus

Cdc25B cooperates with Cdc25A to induce mitosis but has a unique role in activating cyclin B1–Cdk1 at the centrosome

Cdc25 phosphatases are essential for the activation of mitotic cyclin–Cdks, but the precise roles of the three mammalian isoforms (A, B, and C) are unclear. Using RNA interference to reduce the expression of each Cdc25 isoform in HeLa and HEK293 cells, we observed that Cdc25A and -B are both needed for mitotic entry, whereas Cdc25C alone cannot induce mitosis. We found that the G2 delay caused by small interfering RNA to Cdc25A or -B was accompanied by reduced activities of both cyclin B1–Cdk1 and cyclin A–Cdk2 complexes and a delayed accumulation of cyclin B1 protein. Further, three-dimensional time-lapse microscopy and quantification of Cdk1 phosphorylation versus cyclin B1 levels in individual cells revealed that Cdc25A and -B exert specific functions in the initiation of mitosis: Cdc25A may play a role in chromatin condensation, whereas Cdc25B specifically activates cyclin B1–Cdk1 on centrosomes

DNA replication determines timing of mitosis by restricting CDK1 and PLK1 activation

To maintain genome stability, cells need to replicate their DNA before dividing. The kinases CDK1 and PLK1 drive mitotic entry and become active when bulk DNA synthesis is completed at the S/G2 transition. Here, we have tested the hypothesis that DNA replication controls activation of mitotic kinases. Using an optimized double-degron system, we find that human cells unable to initiate DNA replication in S-phase promptly activate CDK1 and PLK1 and prematurely enter mitosis. In the presence of DNA replication, inhibition of CHK1 and p38 leads to premature activation of CDK1 and PLK1. While CDK2 activity promotes DNA replication, activation of CDK1 in S-phase induces severe replication stress. We propose that mitotic kinase activation is governed by a CDK2- and DNA replication-dependent feed-forward loop that ensures timely cell division while preserving genome stability. DNA replication thus functions as a break that coordinates cell cycle activities and determines cell cycle duration

Downstream high-speed plasma jet generation as a direct consequence of shock reformation

Shocks are one of nature's most powerful particle accelerators and have been connected to relativistic electron acceleration and cosmic rays. Upstream shock observations include wave generation, wave-particle interactions and magnetic compressive structures, while at the shock and downstream, particle acceleration, magnetic reconnection and plasma jets can be observed. Here, using Magnetospheric Multiscale (MMS) we show in-situ evidence of high-speed downstream flows (jets) generated at the Earth's bow shock as a direct consequence of shock reformation. Jets are observed downstream due to a combined effect of upstream plasma wave evolution and an ongoing reformation cycle of the bow shock. This generation process can also be applicable to planetary and astrophysical plasmas where collisionless shocks are commonly found. Several mechanisms exist for formation of jets observed in Earth's magnetosheath. Here, the authors show evidence of high-speed downstream flows generated at the Earth's bow shock as a direct consequence of shock reformation, which is different than the proposed mechanisms.Peer reviewe

Dayside response of the magnetosphere to a small shock compression: Van Allen Probes, Magnetospheric MultiScale, and GOES-13.

Observations from Magnetospheric MultiScale (~8 Re) and Van Allen Probes (~5 and 4 Re) show that the initial dayside response to a small interplanetary shock is a double-peaked dawnward electric field, which is distinctly different from the usual bipolar (dawnward and then duskward) signature reported for large shocks. The associated E × B flow is radially inward. The shock compressed the magnetopause to inside 8 Re, as observed by Magnetospheric MultiScale (MMS), with a speed that is comparable to the E × B flow. The magnetopause speed and the E × B speeds were significantly less than the propagation speed of the pulse from MMS to the Van Allen Probes and GOES-13, which is consistent with the MHD fast mode. There were increased fluxes of energetic electrons up to several MeV. Signatures of drift echoes and response to ULF waves also were seen. These observations demonstrate that even very weak shocks can have significant impact on the radiation belts

Structure of the Current Sheet in the 11 July 2017 Electron Diffusion Region Event.

The structure of the current sheet along the Magnetospheric Multiscale (MMS) orbit is examined during the 11 July 2017 Electron Diffusion Region (EDR) event. The location of MMS relative to the X-line is deduced and used to obtain the spatial changes in the electron parameters. The electron velocity gradient values are used to estimate the reconnection electric field sustained by nongyrotropic pressure. It is shown that the observations are consistent with theoretical expectations for an inner EDR in 2-D reconnection. That is, the magnetic field gradient scale, where the electric field due to electron nongyrotropic pressure dominates, is comparable to the gyroscale of the thermal electrons at the edge of the inner EDR. Our approximation of the MMS observations using a steady state, quasi-2-D, tailward retreating X-line was valid only for about 1.4 s. This suggests that the inner EDR is localized; that is, electron outflow jet braking takes place within an ion inertia scale from the X-line. The existence of multiple events or current sheet processes outside the EDR may play an important role in the geometry of reconnection in the near-Earth magnetotail

Near-Earth plasma sheet boundary dynamics during substorm dipolarization.

We report on the large-scale evolution of dipolarization in the near-Earth plasma sheet during an intense (AL ~ -1000 nT) substorm on August 10, 2016, when multiple spacecraft at radial distances between 4 and 15 R E were present in the night-side magnetosphere. This global dipolarization consisted of multiple short-timescale (a couple of minutes) B z disturbances detected by spacecraft distributed over 9 MLT, consistent with the large-scale substorm current wedge observed by ground-based magnetometers. The four spacecraft of the Magnetospheric Multiscale were located in the southern hemisphere plasma sheet and observed fast flow disturbances associated with this dipolarization. The high-time-resolution measurements from MMS enable us to detect the rapid motion of the field structures and flow disturbances separately. A distinct pattern of the flow and field disturbance near the plasma boundaries was found. We suggest that a vortex motion created around the localized flows resulted in another field-aligned current system at the off-equatorial side of the BBF-associated R1/R2 systems, as was predicted by the MHD simulation of a localized reconnection jet. The observations by GOES and Geotail, which were located in the opposite hemisphere and local time, support this view. We demonstrate that the processes of both Earthward flow braking and of accumulated magnetic flux evolving tailward also control the dynamics in the boundary region of the near-Earth plasma sheet.Graphical AbstractMultispacecraft observations of dipolarization (left panel). Magnetic field component normal to the current sheet (BZ) observed in the night side magnetosphere are plotted from post-midnight to premidnight region: a GOES 13, b Van Allen Probe-A, c GOES 14, d GOES 15, e MMS3, g Geotail, h Cluster 1, together with f a combined product of energy spectra of electrons from MMS1 and MMS3 and i auroral electrojet indices. Spacecraft location in the GSM X-Y plane (upper right panel). Colorcoded By disturbances around the reconnection jets from the MHD simulation of the reconnection by Birn and Hesse (1996) (lower right panel). MMS and GOES 14-15 observed disturbances similar to those at the location indicated by arrows

Modeling Kelvin-Helmholtz instability-driven turbulence with hybrid simulations of Alfv\'enic turbulence

Magnetospheric Multiscale (MMS) observations of plasma turbulence generated by a Kelvin-Helmholtz (KH) event at the Earth's magnetopause are compared with a high-resolution two-dimensional (2D) hybrid direct numerical simulation (DNS) of decaying plasma turbulence driven by large-scale balanced Alfv\'enic fluctuations. The simulation, set up with four observation-driven physical parameters (ion and electron betas, turbulence strength, and injection scale) exhibits a quantitative agreement on the spectral, intermittency, and cascade-rate properties with in situ observations, despite the different driving mechanisms. Such agreement demonstrates a certain universality of the turbulent cascade from magnetohydrodynamic (MHD) to sub-ion scales, whose properties are mainly determined by the selected parameters, also indicating that the KH instability-driven turbulence has a quasi-2D nature. The validity of the Taylor hypothesis in the sub-ion spatial range suggests that the fluctuations at sub-ion scales have predominantly low frequencies, consistent with a kinetic Alfv\'en wave-like nature or with quasi-static structures. Finally, the third-order structure function analysis indicates that the cascade rate of the turbulence generated by a KH event in the magnetopause is an order of magnitude larger than in the ambient magnetosheath.Comment: 11 pages, 6 figures, submitted to The Astrophysical Journa