Uma proposta de universidade corporativa pautada nos pilares educação corporativa, gestão do conhecimento e gestão da inovação

Introduction: Due to the constant changes in the corporate scenario, processes and products tend to be rethought. Admitting education as an element of organizational support, this article aims to discuss the importance of creating a Corporate University - UC, based on the Corporate Education - CE, Knowledge Management - KM and Innovation Management - IM pillars. Method: The research adopted a bibliographic review and semi-structured interviews, in a sample of three companies that already have UC. The collected data were submitted to discourse analysis. Results: There was confirmation of the importance of the defined pillars, with definition of steps for the implementation of the UC. Conclusion: The need to align the offer of educational actions to the organization's strategies was realized, protecting the knowledge already built and generating opportunities for innovation in companies, in order to adjust them to the demands of the market and customers. New research is proposed in private and multinational institutions, investigating not only UC practices, but also the peculiarities of KM and IM in different contexts.Introdução: Devido às constantes mudanças do cenário corporativo, processos e produtos tendem a ser repensados. Admitindo a educação como elemento de sustentação organizacional, este artigo visa discutir a importância da criação de uma Universidade Corporativa – UC, com base nos Pilares Educação Corporativa – EC, Gestão do Conhecimento - GC e Gestão da Inovação – GI. Método: A pesquisa adotou a revisão bibliográfica e entrevistas semiestruturadas em uma amostra de três empresas que já possuem UC. Os dados coletados foram submetidos à análise de discurso. Resultados: Houve a confirmação da importância dos Pilares delimitados, com definição de etapas para a concretização da UC. Conclusão: Percebeu-se a necessidade de alinhar a oferta de ações educacionais às estratégias da organização, proteger os conhecimentos já construídos e gerar oportunidades para a inovação nas empresas, a fim de ajustá-las às demandas do mercado e clientes. Propõem-se novas pesquisas em instituições privadas e multinacionais, investigando não somente práticas de UC, mas também as peculiaridades de GC e GI em diferentes contextos

LL-37 boosts immunosuppressive function of placenta-derived mesenchymal stromal cells

Background: Although promising for graft-versus-host disease (GvHD) treatment, MSC therapy still faces important challenges. For instance, increasing MSC migratory capacity as well as potentializing immune response suppression are of interest. For GvHD management, preventing opportunistic infections is also a valuable strategy, since immunocompromised patients are easy targets for infections. LL-37 is a host defense peptide (HDP) that has been deeply investigated due to its immunomodulatory function. In this scenario, the combination of MSC and LL-37 may result in a robust combination to be clinically used. Methods: In the present study, the effects of LL-37 upon the proliferation and migratory capacity of human placenta-derived MSCs (pMSCs) were assessed by MTT and wound scratch assays. The influence of LL-37 over the immunosuppressive function of pMSCs was then investigated using CFSE cell division kit. Flow cytometry and real-time PCR were used to investigate the molecular mechanisms involved in the effects observed. Results: LL-37 had no detrimental effects over MSC proliferation and viability, as assessed by MTT assay. Moreover, the peptide promoted increased migratory behavior of pMSCs and enhanced their immunomodulatory function over activated human PBMCs. Strikingly, our data shows that LL-37 treatment leads to increased TLR3 levels, as shown by flow cytometry, and to an increased expression of factors classically related to immunosuppression, namely IDO, IL-10, TGF-β, IL-6, and IL-1β. Conclusions: Taken together, our observations may serve as groundwork for the development of new therapeutic strategies based on the combined use of LL-37 and MSCs, which may provide patients not only with an enhanced immunosuppression regime, but also with an agent to prevent opportunistic infections

Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi

In earlier studies, we reported that a heterologous prime-boost regimen using recombinant plasmid DNA followed by replication-defective adenovirus vector, both containing Trypanosoma cruzi genes encoding trans-sialidase (TS) and amastigote surface protein (ASP) 2, provided protective immunity against experimental infection with a reticulotropic strain of this human protozoan parasite. Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB/c) mice challenged with myotropic parasite strains (Brazil and Colombian). Initially, we determined that the coadministration during priming of a DNA plasmid containing the murine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptible male mice against acute lethal infections with these parasites. the prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain. Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block. Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by T. cruzi and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Escola Paulista Med, Ctr Terapia Celular & Mol CTCMol, BR-04044010 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044010 São Paulo, BrazilFiocruz MS, Inst Oswaldo Cruz, Lab Biol Celular, BR-21040360 Rio de Janeiro, RJ, BrazilFiocruz MS, Ctr Pesquisas Goncalo Moniz, BR-40296710 Salvador, BA, BrazilHosp Sao Rafael, BR-41253190 Salvador, BA, BrazilUNIFESP, Inst Saude Soc, Dept Biociencias, BR-11015020 Santos, SP, BrazilFiocruz MS, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, BrazilUniv Massachusetts, Sch Med, Dept Med, Div Infect Dis & Immunol, Worcester, MA 01655 USAUniv Fed Santa Catarina, Dept Microbiol Immunol & Parasitol, BR-88040900 Florianopolis, SC, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Ctr Terapia Celular & Mol CTCMol, BR-04044010 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044010 São Paulo, BrazilUNIFESP, Inst Saude Soc, Dept Biociencias, BR-11015020 Santos, SP, BrazilFAPESP: 2009/06820-4FAPESP: 2013/13668/0FAPESP: 2012/22514-3Web of Scienc

Produção e Reintrodução de mudas de Pimenta do reino (Piper nigrum L.) na agricultura familiar de Serraria-PB / Production and Reintroduction of Chili pepper (Piper nigrum L.) seedlings in the family farm of Serraria-PB

A pimenta-do-reino (Piper nigrum L.), cultura de exploração milenar, é uma das especiarias mais importantes e consumidas mundialmente. A microrregião do Brejo Paraibano é uma área promissora na produção de pimenta-do-reino, com climas e solos propícios para a produção; o município de Serraria-PB em particular, possui a prática do cultivo da pimenta-do-reino, porém com o passar do tempo essa cultura foi substituída, devido a dificuldades encontradas para cultivo, colheita e comercialização. Desta maneira, objetivou-se despertar o interesse na reintrodução da pimenta-do-reino como importante cultivar da agricultura local, apresentando a Pimenta-do-reino enquanto vocação ambiental, tecnológica e econômica para a agricultura familiar de Serraria-PB, além de distribuir mudas de pimenta-do-reino com os os mesmos, testando experimentalmente e definindo a melhor forma de propagação assexuada das variedades locais, comparando o potencial do extrato enraizador a base de tiririca com os demais tratamentos. Para isto, o trabalho se caracteriza prioritariamente por duas etapas, uma de socialização de práticas agroecológicas e produção de mudas de pimenta-do-reino, através dos métodos de DRP, e a segunda etapa de experimentação, a qual se subdividiu em dois experimentos, conduzidos por um delineamento inteiramente casualizado (DIC), sob os tratamentos: experimento 1: estacas com e sem nó e diferentes concentrações de extrato de enraizador natural comparado ao enraizador sintético e experimento 2: diferentes concentrações de composto orgânico (0%, 25%, 50% e 75%), com e sem aplicação de microrganismos eficientes. As estacas de pimenta do reino com nó influencia positivamente no comprimento da raiz, contudo, afeta o diâmetro da raiz; não houve diferença significativa nas doses de enraizadores, tanto natural, como sintético, sendo indicado a aplicação de enraizadores naturais; a concentração de composto orgânico acima de 50% prejudica na quantidade de folhas das mudas de pimenta do reino

Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats

Research Areas: Science & Technology - Other TopicsThis study tested the hypothesis that naturally and industrially produced trans-fatty acids can exert distinct efects on metabolic parameters and on gut microbiota of rats. Wistar rats were randomized into three groups according to the diet: CONT-control, with 5% soybean oil and normal amount of fat; HVF-20% of hydrogenated vegetable fat (industrial); and RUM-20% of ruminant fat (natural). After 53 days of treatment, serum biochemical markers, fatty acid composition of liver, heart and adipose tissue, histology and hepatic oxidative parameters, as well as gut microbiota composition were evaluated. HVF diet intake reduced triglycerides (≈ 39.39%) and VLDL levels (≈ 39.49%). Transfatty acids levels in all tissue were higher in HVF group. However, RUM diet intake elevated amounts of anti-infammatory cytokine IL-10 (≈ 14.7%) compared to CONT, but not to HVF. Furthermore, RUM intake led to higher concentrations of stearic acid and conjugated linoleic acid in all tissue; this particular diet was associated with a hepatoprotective efect. The microbial gut communities were signifcantly diferent among the groups. Our results show that ruminant fat reversed the hepatic steatosis normally caused by high fat diets, which may be related to the remodelling of the gut microbiota and its anti-infammatory potential.info:eu-repo/semantics/publishedVersio

Respirometry and Ruminant Nutrition

The gaseous exchange between an organism and the environment is measured by respirometry or indirect calorimetry. Once the oxygen consumption (O2) and the production of carbon dioxide (CO2) and methane (CH4) are known, the energy losses by gas and heat can be calculated. Energy metabolism and methane production have been studied in the Calorimetry and Metabolism Laboratory of the Federal University of Minas Gerais, located in Belo Horizonte, Minas Gerais, Brazil. Animals used are mainly Zebu cattle and their crossbreeds that represent most beef and dairy cattle breed grazed on tropical pastures. System calibration and routine work are addressed in this text. The results obtained on respirometric chambers are expressed in net energy (NE), which can be net energy for maintenance (NEm), lactation (NEL), weight gain (NEg), and pregnancy (NEp). NE is, in fact, what is used by the animal for maintenance and each productive function. The values of k (conversion efficiency of ME into NE) for maintenance (km), milk (kL), weight gain or growth (kg), and pregnancy (kp) are determined. Thanks to the peculiarity of the respirometric technique, the same animal can be evaluated several times, in different physiological states and planes of nutrition

Multidisciplinary approach in breast cancer.

Breast cancer is the most common neoplasm among women. Worldwide, there will be about 2.1 million newly diagnosed female breast cancer cases in 2018, accounting for almost 1 in 4 cancer cases among women. The disease is the most frequently diagnosed cancer in the vast majority of the countries. The purpose of this article is to report the positive experience of a multidisciplinary team in the care of women with breast cancer and their family members. Our approach that is part of the Cancer Patient Support Center (CPSC) at the public health (IPSEMG) in Brazil has been developed taking into account a broader concept of health care. We value not only individual dimensions in patient care, but also common ones, we recognize the importance of dealing with non-biological aspects of the disease, such as socioeconomic, political and cultural facets and our service is focused on health promotion rather than merely on curative treatment. Among the advantages of our approach, we highlight the facilitated accessibility to health services, the patient-centered communication and shared decision making, and the strong bonds between health professionals, patients, and family members. As part of CPSC`s activities, we emphasize the services provided by ?Aconchego? (?Warmth?), that is our breast cancer support group at public health in Brazil

High-Fat Diet Induces Apoptosis of Hypothalamic Neurons

Consumption of dietary fats is amongst the most important environmental factors leading to obesity. In rodents, the consumption of fat-rich diets blunts leptin and insulin anorexigenic signaling in the hypothalamus by a mechanism dependent on the in situ activation of inflammation. Since inflammatory signal transduction can lead to the activation of apoptotic signaling pathways, we evaluated the effect of high-fat feeding on the induction of apoptosis of hypothalamic cells. Here, we show that consumption of dietary fats induce apoptosis of neurons and a reduction of synaptic inputs in the arcuate nucleus and lateral hypothalamus. This effect is dependent upon diet composition, and not on caloric intake, since pair-feeding is not sufficient to reduce the expression of apoptotic markers. The presence of an intact TLR4 receptor, protects cells from further apoptotic signals. In diet-induced inflammation of the hypothalamus, TLR4 exerts a dual function, on one side activating pro-inflammatory pathways that play a central role in the development of resistance to leptin and insulin, and on the other side restraining further damage by controlling the apoptotic activity