Validated Method for the Determination of Five Membered Heterocyclic Polar Compound Imidazole in Drug Substances Using Capillary Electrophoresis and UV Detection

A simple and sensitive method based on capillary electrophoresis with UV detection was developed, optimized, and validated for the determination of five membered heterocyclic polar compound, imidazole in pharmaceutical drug substances. Separation was achieved on bare fused silica capillary using a simple running buffer, potassium dihydrogen phosphate at pH 4.0. Capillary temperature set at 25°C. The applied voltage was 25 kV across the capillary and the samples were injected by hydrodynamically at a pressure of 50 mbar for 5 s. Analyte was monitored at 210 nm. The achieved limit of detection value was 0.005%w/w, limit of quantification value was 0.014%w/w, and the average accuracy value was 98.4% for imidazole. The aim of present study is to develop a specific and sensitive method for the determination of imidazole to overcome the void volume and sample matrix interferences

Cytotoxic and microbial studies of mixed metal complexes

Breast cancer and its treatment have become a prominent and challenging problem today. The increasing multidrug resistance to microbial pathogens is the root cause of breast cancer. Women suffering from cancer showed high levels of E. coli and S. aureus. In the last few decades, there has been a considerable need in the medical field for the discovery of new compounds endowed with antimicrobial activity, despite the fact that several antibiotics and chemotherapy drugs are currently accessible. Substantial research was conducted, particularly on transition complexes as metal-based drugs in pharmacological applications to provide therapeutic options. The synthesis, characterization, antibacterial activity, and cytotoxic activity of copper complexes with specific ligands of amino acids such as tyrosine and arginine are discussed in this work