Boosted output performance of triboelectric nanogenerator via electric double layer effect

For existing triboelectric nanogenerators ( TENGs), it is important to explore unique methods to further enhance the output power under realistic environments to speed up their commercialization. We report here a practical TENG composed of three layers, in which the key layer, an electric double layer, is inserted between a top layer, made of Al/polydimethylsiloxane, and a bottom layer, made of Al. The efficient charge separation in the middle layer, based on Volta's electrophorus, results from sequential contact configuration of the TENG and direct electrical connection of the middle layer to the earth. A sustainable and enhanced output performance of 1.22mA and 46.8 mW cm(-2) under low frequency of 3 Hz is produced, giving over 16-fold enhancement in output power and corresponding to energy conversion efficiency of 22.4%. Finally, a portable power-supplying system, which provides enough d.c. power for charging a smart watch or phone battery, is also successfully developed.ope

On the Role of the Difference in Surface Tensions Involved in the Allosteric Regulation of NHE-1 Induced by Low to Mild Osmotic Pressure, Membrane Tension and Lipid Asymmetry

The sodium-proton exchanger 1 (NHE-1) is a membrane transporter that exchanges Na+ for H+ ion across the membrane of eukaryotic cells. It is cooperatively activated by intracellular protons, and this allosteric regulation is modulated by the biophysical properties of the plasma membrane and related lipid environment. Consequently, NHE-1 is a mechanosensitive transporter that responds to osmotic pressure, and changes in membrane composition. The purpose of this study was to develop the relationship between membrane surface tension, and the allosteric balance of a mechanosensitive transporter such as NHE-1. In eukaryotes, the asymmetric composition of membrane leaflets results in a difference in surface tensions that is involved in the creation of a reservoir of intracellular vesicles and membrane buds contributing to buffer mechanical constraints. Therefore, we took this phenomenon into account in this study and developed a set of relations between the mean surface tension, membrane asymmetry, fluid phase endocytosis and the allosteric equilibrium constant of the transporter. We then used the experimental data published on the effects of osmotic pressure and membrane modification on the NHE-1 allosteric constant to fit these equations. We show here that NHE-1 mechanosensitivity is more based on its high sensitivity towards the asymmetry between the bilayer leaflets compared to mean global membrane tension. This compliance to membrane asymmetry is physiologically relevant as with their slower transport rates than ion channels, transporters cannot respond as high pressure-high conductance fast-gating emergency valves

The WEBT Campaign on the Intermediate BL Lac Object 3C66A in 2007-2008

Prompted by a high optical state in September 2007, the Whole Earth Blazar Telescope (WEBT) consortium organized an intensive optical, near-IR (JHK) and radio observing campaign on the intermediate BL Lac object 3C 66A throughout the fall and winter of 2007 -- 2008. The source remained in a high optical state throughout the observing period and exhibited several bright flares on time scales of ~ 10 days. This included an exceptional outburst around September 15 - 20, 2007, reaching a peak brightness at R ~ 13.4. Our campaign revealed microvariability with flux changes up to |dR/dt| ~ 0.02 mag/hr. Our observations do not reveal evidence for systematic spectral variability or spectral lags. We infer a value of the magnetic field in the emission region of B ~ 19 e_B^{2/7} \tau_h^{-6/7} D_1^{13/7} G. From the lack of systematic spectral variability, we can derive an upper limit on the Doppler factor, D <= 28 \tau_h^{-1/8} e_B^{3/16}. This is in agreement with superluminal motion measurements of \beta_{app} \le 27 and argues against models with very high Lorentz factors of \Gamma > 50, required for a one-zone SSC interpretation of some high-frequency-peaked BL Lac objects detected at TeV gamma-ray energies.Comment: Accepted for publication in The Astrophysical Journa

Urothelial Plaque Formation in Post-Golgi Compartments

Urothelial plaques are specialized membrane domains in urothelial superficial (umbrella) cells, composed of highly ordered uroplakin particles. We investigated membrane compartments involved in the formation of urothelial plaques in mouse umbrella cells. The Golgi apparatus did not contain uroplakins organized into plaques. In the post-Golgi region, three distinct membrane compartments containing uroplakins were characterized: i) Small rounded vesicles, located close to the Golgi apparatus, were labelled weakly with anti-uroplakin antibodies and they possessed no plaques; we termed them “uroplakin-positive transporting vesicles” (UPTVs). ii) Spherical-to-flattened vesicles, termed “immature fusiform vesicles” (iFVs), were uroplakin-positive in their central regions and contained small urothelial plaques. iii) Flattened “mature fusiform vesicles” (mFVs) contained large plaques, which were densely labelled with anti-uroplakin antibodies. Endoytotic marker horseradish peroxidase was not found in these post-Golgi compartments. We propose a detailed model of de novo urothelial plaque formation in post-Golgi compartments: UPTVs carrying individual 16-nm particles detach from the Golgi apparatus and subsequently fuse into iFV. Concentration of 16-nm particles into plaques and removal of uroplakin-negative membranes takes place in iFVs. With additional fusions and buddings, iFVs mature into mFVs, each carrying two urothelial plaques toward the apical surface of the umbrella cell

Electrochemically synthesized polymers in molecular imprinting for chemical sensing

This critical review describes a class of polymers prepared by electrochemical polymerization that employs the concept of molecular imprinting for chemical sensing. The principal focus is on both conducting and nonconducting polymers prepared by electropolymerization of electroactive functional monomers, such as pristine and derivatized pyrrole, aminophenylboronic acid, thiophene, porphyrin, aniline, phenylenediamine, phenol, and thiophenol. A critical evaluation of the literature on electrosynthesized molecularly imprinted polymers (MIPs) applied as recognition elements of chemical sensors is presented. The aim of this review is to highlight recent achievements in analytical applications of these MIPs, including present strategies of determination of different analytes as well as identification and solutions for problems encountered

Gene Expression during the Generation and Activation of Mouse Neutrophils: Implication of Novel Functional and Regulatory Pathways

As part of the Immunological Genome Project (ImmGen), gene expression was determined in unstimulated (circulating) mouse neutrophils and three populations of neutrophils activated in vivo, with comparison among these populations and to other leukocytes. Activation conditions included serum-transfer arthritis (mediated by immune complexes), thioglycollate-induced peritonitis, and uric acid-induced peritonitis. Neutrophils expressed fewer genes than any other leukocyte population studied in ImmGen, and down-regulation of genes related to translation was particularly striking. However, genes with expression relatively specific to neutrophils were also identified, particularly three genes of unknown function: Stfa2l1, Mrgpr2a and Mrgpr2b. Comparison of genes up-regulated in activated neutrophils led to several novel findings: increased expression of genes related to synthesis and use of glutathione and of genes related to uptake and metabolism of modified lipoproteins, particularly in neutrophils elicited by thioglycollate; increased expression of genes for transcription factors in the Nr4a family, only in neutrophils elicited by serum-transfer arthritis; and increased expression of genes important in synthesis of prostaglandins and response to leukotrienes, particularly in neutrophils elicited by uric acid. Up-regulation of genes related to apoptosis, response to microbial products, NFkB family members and their regulators, and MHC class II expression was also seen, in agreement with previous studies. A regulatory model developed from the ImmGen data was used to infer regulatory genes involved in the changes in gene expression during neutrophil activation. Among 64, mostly novel, regulatory genes predicted to influence these changes in gene expression, Irf5 was shown to be important for optimal secretion of IL-10, IP-10, MIP-1α, MIP-1β, and TNF-α by mouse neutrophils in vitro after stimulation through TLR9. This data-set and its analysis using the ImmGen regulatory model provide a basis for additional hypothesis-based research on the importance of changes in gene expression in neutrophils in different conditions

The whole earth blazar telescope campaign on the intermediate BL Lac object 3C 66A in 2007-2008

Prompted by a high optical state in 2007 September, the Whole Earth Blazar Telescope consortium organized an intensive optical, near-IR (JHK) and radio observing campaign on the intermediate BL Lac object 3C 66A throughout the fall and winter of 2007-2008. In this paper, we present data from 28 observatories in 12 countries, covering the observing season from late 2007 July through 2008 February. The source remained in a high optical state throughout the observing period and exhibited several bright flares on timescales of 10 days. This included an exceptional outburst around 2007 September 15-20, reaching a peak brightness at R 13.4. Our campaign revealed microvariability with flux changes up to |dR/dt| 0.02 mag hr-1. Our observations do not reveal evidence for systematic spectral variability in the overall high state covered by our campaign, in agreement with previous results. In particular, we do not find evidence for spectral hysteresis in 3C 66A for which hints were found in an earlier campaign in a somewhat lower flux state. We also did not find any evidence for spectral lags in the discrete correlation functions between different optical bands. We infer a value of the magnetic field in the emission region of B 19 e 2/7B τ-6/7h D 13/71 G, where eB is the magnetic field equipartition fraction, τh is the shortest observed variability timescale in units of hours, and D 1 is the Doppler factor in units of 10. From the lack of systematic spectral variability, we can derive an upper limit on the Doppler factor, D ≤ 28 τ-1/8h e 3/16B. This is in perfect agreement with superluminal motion measurements with the VLBI/VLBA of βapp ≤ 27 and argues against models with very high Lorentz factors of Γ ≳ 50, required for a one-zone synchrotron-self-Compton interpretation of some high-frequency-peaked BL Lac objects detected at TeV γ-ray energies. © 2009 The American Astronomical Society

Motivational Interviewing as Evidence-Based Practice? An Example from Sexual Risk Reduction Interventions Targeting Adolescents and Young Adults

This paper critically examines sexual risk reduction interventions, more specifically how they are evaluated and the implications that this has for sexual health policy. The focus is on motivational interviewing (MI) interventions which aim to promote protective behaviors related to sexual risk on the part of young people. MI has become increasingly popular, largely due to it being a highly flexible counseling approach that may, with adequate staff training, and fidelity in implementation, be tailored to many different settings (e.g., health care, schools and in community work). Following a scoping review that comprised 34 papers, of which 29 were unique studies, the range and type of existing research were examined. The results show a wide range of study designs and evaluation procedures, MI conceptualizations, modes of MI delivery, and the particular sub-populations of youth and sexual risk behaviors targeted. While this makes it difficult to draw any generalized conclusions about “what works” in prevention, it provides important insights about the complexity of sexual risk behavior as well as complex behavioral treatment approaches like MI. We therefore problematize the political drive to implement evidence-based methods without adequate resource allocation and contextual adaptation