212 research outputs found

    The Predictive Potentiality of Salivary Microbiome for the Recurrence of Early Childhood Caries

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    The aim of this study was to investigate the variation of the salivary microbiota in the recurrence of early childhood caries (ECC), and to explore and verify the potential microbial indicators of ECC recurrence. Saliva samples from kindergarten children were tracked every 6 months for 1 year. Finally, in total 28 children and 84 samples were placed on the analysis phase: 7 children with ECC recurrence made up the ECC-recurrence (ER) group, 6 children without ECC recurrence constituted the non-ECC-recurrence (NER) group, and 15 children who kept ECC-free were set as the ECC-free (EF) group. DNA amplicons of the V3-V4 hypervariable region of the bacterial 16S rDNA were generated and sequencing was performed using Illumina MiSeq PE250 platform. No statistically significant differences of the Shannon indices were found in both cross-sectional and longitudinal comparisons. Furthermore, both principal coordinates analysis (PCoA) and heatmap plots demonstrated that the salivary microbial community structure might have potentiality to predict ECC recurrence at an early phase. The relative abundance of Fusobacterium, Prevotella, Leptotrichia, and Capnocytophaga differed significantly between the ER and NER groups at baseline. The values of area under the curve (AUC) of the four genera and their combined synthesis in the prediction for ECC recurrence were 0.857, 0.833, 0.786, 0.833, and 0.952, respectively. The relative abundance of Fusobacterium, Prevotella, Leptotrichia, and Capnocytophaga and their combination showed satisfactory accuracy in the prediction for ECC recurrence, indicating that salivary microbiome had predictive potentiality for recurrence of this disease. These findings might facilitate more effective strategy to be taken in the management of the recurrence of ECC

    Distinct Salmonella Enteritidis lineages associated with enterocolitis in high-income settings and invasive disease in low-income settings.

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    An epidemiological paradox surrounds Salmonella enterica serovar Enteritidis. In high-income settings, it has been responsible for an epidemic of poultry-associated, self-limiting enterocolitis, whereas in sub-Saharan Africa it is a major cause of invasive nontyphoidal Salmonella disease, associated with high case fatality. By whole-genome sequence analysis of 675 isolates of S. Enteritidis from 45 countries, we show the existence of a global epidemic clade and two new clades of S. Enteritidis that are geographically restricted to distinct regions of Africa. The African isolates display genomic degradation, a novel prophage repertoire, and an expanded multidrug resistance plasmid. S. Enteritidis is a further example of a Salmonella serotype that displays niche plasticity, with distinct clades that enable it to become a prominent cause of gastroenteritis in association with the industrial production of eggs and of multidrug-resistant, bloodstream-invasive infection in Africa.This work was supported by the Wellcome Trust. We would like to thank the members of the Pathogen Informatics Team and the core sequencing teams at the Wellcome Trust Sanger Institute (Cambridge, UK). We are grateful to D. Harris for work in managing the sequence data

    Study of CP violation in B0 → DK⋆(892)0 decays with D → Kπ(ππ), ππ(ππ), and KK final states

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    A measurement of CP-violating observables associated with the interference of B0 → D0K⋆ (892)0 and B0 → D¯ 0K⋆ (892)0 decay amplitudes is performed in the D0 → K∓π ±(π +π −), D0 → π +π −(π +π −), and D0 → K+K− fnal states using data collected by the LHCb experiment corresponding to an integrated luminosity of 9 fb−1 . CP-violating observables related to the interference of B0 s → D0K¯ ⋆ (892)0 and B0 s → D¯ 0K¯ ⋆ (892)0 are also measured, but no evidence for interference is found. The B0 observables are used to constrain the parameter space of the CKM angle γ and the hadronic parameters r DK⋆ B0 and δ DK⋆ B0 with inputs from other measurements. In a combined analysis, these measurements allow for four solutions in the parameter space, only one of which is consistent with the world average

    Parameter Sensitivity Analysis and Optimization of the Single-Layer Urban Canopy Model in the Megacity of Shanghai

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    In order to meet the demand of more refined urban weather forecast, it is of great practical significance to improve and optimize the single-layer urban canopy model (SLUCM) suitable for the megacity of Shanghai. In this paper, based on the offline SLUCM model driven by a whole-year surface flux observation data in the Shanghai central business district, a series of parameter sensitivity tests are carried out by using the one at a time (OAT) method, the relative importance and a set of optimized parameters of the SLUCM suitable for high-density urban area are established, and the improvement of simulation is evaluated. The results show that SLUCM well reproduces the seasonal mean diurnal patterns of the net all-wave radiation flux (Q∗) and sensible heat flux (QH) but underestimates their magnitudes. Both Q∗ and QH are linearly sensitive to the albedo, and most sensitive to the roof albedo, the second to the wall albedo, but relatively insensitive to the road albedo. The sensitivity of Q∗ and QH to emissivity is not as strong as that of albedo, and the variation trend is also linear. Similar to albedo, Q∗ and QH are most sensitive to roof emissivity. The effect of thermal parameters (heat capacity and conductivity) on fluxes is logarithmic. The sensitivity of surface fluxes to geometric parameters has no specific variation pattern. After parameter optimization, RMSE of Q∗ decreases by about 3.4–18.7 Wm−2 in four seasons. RMSE of the longwave radiation (L↑) decreases by about 1.2–7.87 Wm−2. RMSE of QH decreases by about 2–5 Wm−2. This study provides guidance for future development of the urban canopy model parameterizations and urban climate risk response

    The weakening effect of urbanization on tropical cyclone surface winds : An observational study for Shanghai

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    The observation-reanalysis difference method (OMR) and wind profile fitting method were used to analyze the influence urbanization has on the near-surface wind speed in Shanghai during tropical cyclone events. The data used include daily wind speed data from the Shanghai Meteorological Observatory Station collected from 1991 to 2020, hourly wind speed data from 100 m high wind towers from 2017 to 2019, and reanalysis data that covered the same period. The results indicated that during tropical cyclone occurrence, the wind speed of the OMR in the central urban region was significantly lower than that in the suburban area, and the OMR declined more obviously over the year, down to −8 m/s in 2020. Urbanization leads to the increase of average wind weakening rate to be higher than the maximum wind weakening rate, causing the maximum weakening rate of the central urban region to the average wind over 80%, while maximum wind speed was less than 80%. The weakening rate of both the average and maximum wind speeds during tropical cyclone impacts is greater than the rate that the annual average wind speed was reduced. A logarithmic variation was visible in the wind profile of the island station during tropical cyclone occurrence, with an average friction velocity U∗ of 0.9389 m/s and an average rough length Z0 of 0.4915 m. The wind speed during tropical cyclone events was higher than the three-year average wind speed within each layer. The suburban stations show a linear variation, and every hundred meters of height results in an increase of 5–6 m/s in the wind speed change rate. At 100 m of altitude, the wind speed in the suburban region is reduced by approximately 40%

    Numerical Studies on the Influence of Building Morphology on Urban Canopy Wind Speed

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    Abstract Buildings increase the urban surface roughness and reduce near‐surface wind speeds in the urban canopy due to the drag effect. Urban heat storage and other effects cause urban warming as well, which decreases the urban boundary layer stability and enhances the turbulence exchange between upper and lower layer. As upper momentum is transported downward, the wind speed of urban canopy increases. Quantitative descriptions of these mechanisms are still lacking currently. This paper presents high‐resolution numerical simulation results of a mega city, Shanghai, China from 2016 to 2020 using the building effect parameterization in WRF (WRF‐BEP) with urban morphological parameters. The dynamic and thermal effects of building morphology on urban canopy wind speed were separated and their quantitative expression functions were given. The results indicate that the influence of building morphology on urban canopy wind speed is mainly dynamic resulting in a wind speed attenuation of approximately 50% and nearly constant. The thermal effect of building morphology on urban canopy wind speed increases with the urban heat island intensity, and the thermal effect could increase urban canopy wind speed by about 30% under the condition of strong urban heat island. The relative contributions of the dynamic and thermal effects of building morphology to urban canopy wind speed change with the wind speed. As wind speed increases, the contribution of the thermal effect of building morphology to urban canopy wind speed gradually decreases. This paper provides a quantitative relationship between the urban canopy wind speed variation and urban morphology, as well as urban heat island intensity
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