Coal monthly bulletin Special Number/1977

Using hundreds of XMM-Newton and Chandra archival observations and nearly a thousand RXTE observations, we have generated a comprehensive library of the known pulsars in the Small and Large Magellanic Clouds (SMC and LMC). The pulsars are detected multiple times across the full parameter spaces of X-ray luminosity (LX = 1031 − 38 erg s− 1) and spin period (P < 1 s to P > 1,000 s), and the library enables time-domain studies at a range of energy scales. The high time resolution and sensitivity of the European Photon Imaging cameras are complemented by the angular resolution of Chandra and the regular monitoring of RXTE. Our processing pipeline uses the latest calibration files and software to generate a suite of useful products for each pulsar detection: event lists, high-time-resolution light curves, periodograms, spectra, and complete histories of math formula, the pulsed fraction, and so on, in the broad (0.2–12 keV), soft (0.2–2 keV), and hard (2–12 keV) energy bands. After combining the observations from these telescopes, we found that 27 pulsars show long-term spin up and 24 long-term spin down. We also used the faintest and brightest sources to map out the lower and upper boundaries of accretion-powered X-ray emission: the propeller line and the Eddington line, respectively. We are in the process of comparing the observed pulse profiles to geometric models of X-ray emission in order to constrain the physical parameters of the pulsars. Finally, we are preparing a public release of the library so that it can be used by others in the astronomical community

The XMM-Newton survey of the Small Magellanic Cloud: XMMUJ005011.2-730026 = SXP214, a Be/X-ray binary pulsar

In the course of the XMM-Newton survey of the Small Magellanic Cloud (SMC), a region to the east of the emission nebula N19 was observed in November 2009. To search for new candidates for high mass X-ray binaries the EPIC PN and MOS data of the detected point sources were investigated and their spectral and temporal characteristics identified. A new transient (XMMUJ005011.2-730026= SXP214) with a pulse period of 214.05 s was discovered; the source had a hard X-ray spectrum with power-law index of ~0.65. The accurate X-ray source location permits the identification of the X-ray source with a ~15th magnitude Be star, thereby confirming this system as a new Be/X-ray binary.Comment: 8 pages 11 figures. Accepted for publication in MNRA

The Search for High-Mass X-ray Binaries in the Phoenix Dwarf Galaxy

We report on the first X-ray images of the Phoenix dwarf galaxy, taken with \emph{XMM-Newton} in July 2009. This local group dwarf galaxy shares similarities with the Small Magellanic Cloud (SMC) including a burst of star formation $\sim$50 Myr ago. The SMC has an abundance of High Mass X-ray Binaries (HMXBs) and so we have investigated the possibility of an HMXB population in Phoenix with the intention of furthering the understanding of the HMXB-star formation rate relation. The data from the combined European Photon Imaging Cameras (EPIC) were used to distinguish between different source classes (foreground stars, background galaxies, AGN and supernova remnants) using EPIC hardness ratios and correlations with optical and radio catalogues. Of the 81 X-ray sources in the field of view, six are foreground stars, four are galaxies and one is an AGN. The remaining sources with optical counterparts have log($\frac{f_X}{f_{opt}}$) consistent with AGN in the local universe. Further investigation of five sources in the field of view suggests they are all background AGN. Their position behind the gas cloud associated with Phoenix makes them a possible tool for further probing the metallicity of this region. We find no evidence for any HMXBs in Phoenix at this time. This rules out the existence of the X-ray persistent supergiant X-ray binary systems. However the transient nature of the Be/X-ray binaries means we cannot rule out a population of these sources but can conclude that it is not extensive.Comment: 13 pages, 4 figures, 4 tables, Accepted for publication in MNRA

The Orbital Solution and Spectral Classification of the High-Mass X-Ray Binary IGR J01054-7253 in the Small Magellanic Cloud

We present X-ray and optical data on the Be/X-ray binary (BeXRB) pulsar IGR J01054-7253 = SXP11.5 in the Small Magellanic Cloud (SMC). Rossi X-ray Timing Explorer (RXTE) observations of this source in a large X-ray outburst reveal an 11.483 +/- 0.002s pulse period and show both the accretion driven spin-up of the neutron star and the motion of the neutron star around the companion through Doppler shifting of the spin period. Model fits to these data suggest an orbital period of 36.3 +/- 0.4d and Pdot of (4.7 +/- 0.3) x 10^{-10} ss^{-1}. We present an orbital solution for this system, making it one of the best described BeXRB systems in the SMC. The observed pulse period, spin-up and X-ray luminosity of SXP11.5 in this outburst are found to agree with the predictions of neutron star accretion theory. Timing analysis of the long-term optical light curve reveals a periodicity of 36.70 +/- 0.03d, in agreement with the orbital period found from the model fit to the X-ray data. Using blue-end spectroscopic observations we determine the spectral type of the counterpart to be O9.5-B0 IV-V. This luminosity class is supported by the observed V-band magnitude. Using optical and near-infrared photometry and spectroscopy, we study the circumstellar environment of the counterpart in the months after the X-ray outburst.Comment: 12 pages, 13 figures and 3 tables. This paper has been accepted for publication in MNRA

Μαγγελάνος (ιταλ. Magellano) / Fabio Turchetti και Luca Congedo

Μετάφραση από τα ιταλικά θεατρικού έργο των Fabio Turchetti και Luca Congedo  βασισμένο σε χειρόγραφο του Antonio Pigafetta, με τίτλο “Relazione del primo viaggio intorno al mondo”, όπου περιγράφεται λεπτομερώς η πρώτη αποστολή περίπλου της Γης του Μαγγελάνου. Στόχος του κειμένου είναι η ανάδειξη του συναισθηματικού κόσμου και της εσωτερικής πάλης που βίωσε ο Μαγγελάνος ανάμεσα στις καθιερωμένες αξίες που είχε ο ίδιος ως Ευρωπαίος πολίτης του 16ου αι. και στην επαφή του με το διαφορετικό του νέου κόσμου που ανακαλύπτει.  Το έργο μεταφράστηκε για τις ανάγκες μουσικοθεατρικής παράστασης  που δόθηκε στα Ιωάννινα, με την υποστήριξη του Πνευματικού Κέντρου Ιωαννίνων και της Περιφέρειας της Απουλίας (Ιταλία) με τη συμμετοχή των: Luca Congedo, Fabio Turchetti, Vincenzo Urso, Hermes Mangialardo και Ελένης Σοκορέλη

An experimentally validated network of nine haematopoietic transcription factors reveals mechanisms of cell state stability.

Transcription factor (TF) networks determine cell-type identity by establishing and maintaining lineage-specific expression profiles, yet reconstruction of mammalian regulatory network models has been hampered by a lack of comprehensive functional validation of regulatory interactions. Here, we report comprehensive ChIP-Seq, transgenic and reporter gene experimental data that have allowed us to construct an experimentally validated regulatory network model for haematopoietic stem/progenitor cells (HSPCs). Model simulation coupled with subsequent experimental validation using single cell expression profiling revealed potential mechanisms for cell state stabilisation, and also how a leukaemogenic TF fusion protein perturbs key HSPC regulators. The approach presented here should help to improve our understanding of both normal physiological and disease processes.Research in the authors’ laboratories was supported by Bloodwise, The Wellcome Trust, Cancer Research UK, the Biotechnology and Biological Sciences Research Council, the National Institute of Health Research, the Medical Research Council, the MRC Molecular Haematology Unit (Oxford) core award, a Weizmann-UK “Making Connections” grant (Oxford) and core support grants by the Wellcome Trust to the Cambridge Institute for Medical Research (100140) and Wellcome Trust–MRC Cambridge Stem Cell Institute (097922).This is the final version of the article. It first appeared from eLife via http://dx.doi.org/10.7554/eLife.1146

The XMM-Newton survey of the Small Magellanic Cloud: XMMUJ010633.1-731543 and XMMUJ010743.1-715953, two new Be/X-ray binary systems

In the course of the XMM-Newton survey of the Small Magellanic Cloud (SMC), two new bright X-ray sources were discovered exhibiting the spectral characteris- tics of High Mass X-ray Binaries - but revealing only weak evidence for pulsations in just one of the objects(at 153s in XMMUJ010743.1-715953). The accurate X- ray source locations permit the identification of these X-ray source with Be stars, thereby strongly suggesting these systems are new Be/X-ray binaries. From blue spectra the proposed classification for XMMUJ010633.1-731543 is B0.5-1Ve and for XMMUJ010743.1-715953 it is B2IV-Ve.Comment: MNRAS (accepted), 12 pages, 17 figures, 4 table

Single-cell analyses of regulatory network perturbations using enhancer-targeting TALEs suggest novel roles for PU.1 during haematopoietic specification.

Transcription factors (TFs) act within wider regulatory networks to control cell identity and fate. Numerous TFs, including Scl (Tal1) and PU.1 (Spi1), are known regulators of developmental and adult haematopoiesis, but how they act within wider TF networks is still poorly understood. Transcription activator-like effectors (TALEs) are a novel class of genetic tool based on the modular DNA-binding domains of Xanthomonas TAL proteins, which enable DNA sequence-specific targeting and the manipulation of endogenous gene expression. Here, we report TALEs engineered to target the PU.1-14kb and Scl+40kb transcriptional enhancers as efficient new tools to perturb the expression of these key haematopoietic TFs. We confirmed the efficiency of these TALEs at the single-cell level using high-throughput RT-qPCR, which also allowed us to assess the consequences of both PU.1 activation and repression on wider TF networks during developmental haematopoiesis. Combined with comprehensive cellular assays, these experiments uncovered novel roles for PU.1 during early haematopoietic specification. Finally, transgenic mouse studies confirmed that the PU.1-14kb element is active at sites of definitive haematopoiesis in vivo and PU.1 is detectable in haemogenic endothelium and early committing blood cells. We therefore establish TALEs as powerful new tools to study the functionality of transcriptional networks that control developmental processes such as early haematopoiesis.Research in the authors’ laboratories was supported by Leukaemia and Lymphoma Research, The Wellcome Trust, Cancer Research UK, the Biotechnology and Biological Sciences Research Council, the National Institute of Health Research, the Medical Research Council and core support grants by the Wellcome Trust to the Cambridge Institute for Medical Research and Wellcome Trust–MRC Cambridge Stem Cell Institute. V.K.S.K. was supported by a Japan Society for the Promotion of Science (JSPS) Research Fellowship for Young Scientists.This is the final version. It was first published by the Company of Biologists http://dev.biologists.org/content/141/20/4018.long

Polygenic Risk Scores for Prediction of Breast Cancer and Breast Cancer Subtypes

Stratification of women according to their risk of breast cancer based on polygenic risk scores (PRSs) could improve screening and prevention strategies. Our aim was to develop PRSs, optimized for prediction of estrogen receptor (ER)-specific disease, from the largest available genome-wide association dataset and to empirically validate the PRSs in prospective studies. The development dataset comprised 94,075 case subjects and 75,017 control subjects of European ancestry from 69 studies, divided into training and validation sets. Samples were genotyped using genome-wide arrays, and single-nucleotide polymorphisms (SNPs) were selected by stepwise regression or lasso penalized regression. The best performing PRSs were validated in an independent test set comprising 11,428 case subjects and 18,323 control subjects from 10 prospective studies and 190,040 women from UK Biobank (3,215 incident breast cancers). For the best PRSs (313 SNPs), the odds ratio for overall disease per 1 standard deviation in ten prospective studies was 1.61 (95%CI: 1.57-1.65) with area under receiver-operator curve (AUC) = 0.630 (95%CI: 0.628-0.651). The lifetime risk of overall breast cancer in the top centile of the PRSs was 32.6%. Compared with women in the middle quintile, those in the highest 1% of risk had 4.37- and 2.78-fold risks, and those in the lowest 1% of risk had 0.16- and 0.27-fold risks, of developing ER-positive and ER-negative disease, respectively. Goodness-of-fit tests indicated that this PRS was well calibrated and predicts disease risk accurately in the tails of the distribution. This PRS is a powerful and reliable predictor of breast cancer risk that may improve breast cancer prevention programs.NovartisEli Lilly and CompanyAstraZenecaAbbViePfizer UKCelgeneEisaiGenentechMerck Sharp and DohmeRocheCancer Research UKGovernment of CanadaArray BioPharmaGenome CanadaNational Institutes of HealthEuropean CommissionMinistère de l'Économie, de l’Innovation et des Exportations du QuébecSeventh Framework ProgrammeCanadian Institutes of Health Researc