Diseño electrónico con panel solar para extender la vida de un nodo en una red inalámbrica de sensores (WSN)

En el presente trabajo se realizó el diseño de un sistema de recolección de energía de bajo costo, con un panel solar fotovoltaico (tipo monocristalino) y baterías recargables Ni-MH para lograr la autonomía energética de una placa NodeMCU v1.0/V3, dispositivo que es ideal para aplicaciones en redes WSN, ya que cuenta con un módulo Wi-Fi ESP-12E, para la comunicación inalámbrica con otros nodos. Por medio de instrumentos de medición como osciloscopios y multímetros se determina la corriente promedio con la que funciona el NodeMCU y con este valor se realizó el diseño del sistema de recolección de energía con el panel solar y baterías Ni-MH. Tras varias pruebas de funcionamiento se obtuvieron resultados exitosos al lograr el funcionamiento continuo (auto sostenible) del NodeMCU, para su uso en múltiples aplicaciones de IoT (Internet of Things).Actividad solar

Differential Engraftment of Parental A20 PD-L1 WT and PD-L1 KO Leukemia Cells in Semiallogeneic Recipients in the Context of PD-L1/PD-1 Interaction and NK Cell-Mediated Hybrid Resistance

[EN] The contribution of natural killer (NK) cells to tumor rejection in the context of programmed death-ligand 1/programmed death 1 (PD-L1/PD-1) blockade is a matter of intense debate. To elucidate the role of PD-L1 expression on tumor cells and the functional consequences of engaging PD-1 receptor on cytotoxic cells, PD-L1 expression was genetically inactivated and WT or PD-L1-deficient parental tumor cells were adoptively transferred intravenously into F1 recipients. The engraftment of PD-L1-deficient A20 tumor cells in the spleen and liver of F1 recipients was impaired compared with A20 PD-L1 WT tumor counterparts. To elucidate the mechanism responsible for this differential tumor engraftment and determine the relevance of the role of the PD-L1/PD-1 pathway in the interplay of tumor cells/NK cells, a short-term competitive tumor implantation assay in the peritoneal cavity of semiallogeneic F1 recipients was designed. The results presented herein showed that NK cells killed target tumor cells with similar efficiency regardless of PD-L1 expression, whereas PD-L1 expression on A20 tumor cells conferred significant tumor protection against rejection by CD8 T cells confirming the role of the co-inhibitory receptor PD-1 in the modulation of their cytotoxic activity. In summary, PD-L1 expression on A20 leukemia tumor cells modulates CD8 T-cell-mediated responses to tumor-specific antigens but does not contribute to inhibit NK cell-mediated hybrid resistance, which correlates with the inability to detect PD-1 expression on NK cells neither under steady-state conditions nor under inflammatory conditions.S

Chemotactic TEG3 Cells' Guiding Platforms Based on PLA Fibers Functionalized With the SDF-1 alpha/CXCL12 Chemokine for Neural Regeneration Therapy

Following spinal cord injury, olfactory ensheathing cell (OEC) transplantation is a promising therapeutic approach in promoting functional improvement. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical concentration differences. Here we compare the attachment, morphology, and directionality of an OEC-derived cell line, TEG3 cells, seeded on functionalized nanoscale meshes of Poly(l/dl-lactic acid; PLA) nanofibers. The size of the nanofibers has a strong effect on TEG3 cell adhesion and migration, with the PLA nanofibers having a 950 nm diameter being the ones that show the best results. TEG3 cells are capable of adopting a bipolar morphology on 950 nm fiber surfaces, as well as a highly dynamic behavior in migratory terms. Finally, we observe that functionalized nanofibers, with a chemical concentration increment of SDF-1α/CXCL12, strongly enhance the migratory characteristics of TEG3 cells over inhibitory substrates

Tau aggregation

Here we revisit tau protein aggregation at primary, secondary, tertiary and quaternary structures. In addition, the presence of non-aggregated tau protein, which has been recently discovered, is also commented o

Abating the Feminization of Poverty: Changing the Rules Governing Post - Decree Modification of Child Support Obligations

El crecimiento estimado para el mercado mundial de espacios de coworking será de 17% para el año 2020, respecto del año anterior (Desmag, 2018), lo cual muestra el dinamismo de esta nueva forma de trabajar a nivel mundial. En el 2013 se registró la primera empresa de coworking en el Perú denominada Comunal, luego ingresaron al mercado otras marcas internacionales y locales. El punto en común entre todas las opciones actuales es que sus modelos de negocios están enfocados en generar comodidad, modernidad y ahorro a sus arrendatarios.  El presente Plan de Negocios tiene como objetivo desarrollar una nueva propuesta de espacios compartidos, denominada Prime Coworking. Este espacio generará ahorro a emprendedores, pequeñas y medianas empresas, siguiendo las tendencias locales e internacionales. Esto se logrará ofreciendo ambientes cómodos, modernos e implementados para la actividad empresarial, y sobre todo que contribuyan a incentivar la retención de sus trabajadores mediante capacitaciones en habilidades blandas. Por último, con la evaluación del proyecto se concluye que el negocio es viable, dado que para una inversión total de 1 007 160 soles se genera un valor actual neto de 338 mil soles. La inversión será financiada con un aporte de los socios de 705 010 soles que generará un valor para el accionista de 311 000 soles, para un periodo de valoración de 5 años y con un payback de 3.6 años.Coworking spaces global market growth is estimated to be 17% YoY by 2020, (Desmag, 2018), which shows the increasing demand for this new way of working worldwide. In 2013, the first coworking company in Peru called Comunal was registered, then other international and local brands entered the local market. The common point among all market competitors is that their business models are focused on generating comfort, a trendy style and operating savings to their tenants. This Business Plan aims to develop a new proposal for shared spaces, called Prime Coworking, that follows local and international trends and generates savings to entrepreneurs, small and medium enterprises. It does this by offering comfortable, modern and fully implemented environments for doing business and especially by encouraging the engagement of their employees through training in soft skills. Lastly, after our project evaluation it is concluded that the business is profitable, given a total investment of 1 007 160 soles, and an NPV of 338 thousand soles. This investment will be financed with equity of 705 010 soles in shares that will generate a shareholder value of 311 000 soles, for a period of 5 years with a payback of 3.6 years.Trabajo de investigació

Impact of moderate coronary atherosclerosis on long-term left ventricular remodeling after aortic valve replacement

Background: The role of coronary atherosclerosis (CA+) in ventricular remodeling after aortic valve replacement (AVR) for isolated aortic stenosis (AS) is not well defined. We sought to evaluate the impact of not revascularized moderate coronary atherosclerosis in long-term left ventricular (LV) remodeling after AVR. Methods: We assessed by coronariography the coronary artery disease in 66 patients referred for AVR and evaluated morphological and functional LV data by echocardiography both preoperatively and postoperatively (3 ± 1.2 years). Results: In patients without coronary atherosclerosis, hypertrophy regression was more intense and the absolute reverse remodeling was higher in LV mass index (–55.8 ± 36 g/m2 vs –28.4 ± 34 g/m2, p = 0.004), reduction of LV dimensions (LV end-diastolic diameter [LVEDD]: –4.1 ± 7.4 mm vs –2.2 ± 8.3 mm, p = 0.04), and regression of wall thickness (interventricular septum [IVS]: –3.3 ± 2.6 mm vs –1.6 ± 2.2 mm, p = 0.01; and posterior wall thickness [PWT]: –2.1 ± 2.1 mm vs 0.6 ± 2.1 mm, p = 0.012). Conclusions: After AVR for AS, not revascularized moderate coronary atherosclerosis determines a long-term lesser degree of LV hypertrophy regression and a worse absolute reverse remodeling of LV mass index, LVEDD, IVS and PWT. (Cardiol J 2011; 18, 3: 277–281

New functions of Semaphorin 3E and its receptor PlexinD1 during developing and adult hippocampal formation

The development and maturation of cortical circuits relies on the coordinated actions of long and short range axonal guidance cues. In this regard, the class 3 semaphorins and their receptors have been seen to be involved in the development and maturation of the hippocampal connections. However, although the role of most of their family members have been described, very few data about the participation of Semaphorin 3E (Sema3E) and its receptor PlexinD1 during the development and maturation of the entorhino-hippocampal (EH) connection are available. In the present study, we focused on determining their roles both during development and in adulthood. We determined a relevant role for Sema3E/PlexinD1 in the layer-specific development of the EH connection. Indeed, mice lacking Sema3E/PlexinD1 signalling showed aberrant layering of entorhinal axons in the hippocampus during embryonic and perinatal stages. In addition, absence of Sema3E/PlexinD1 signalling results in further changes in postnatal and adult hippocampal formation, such as numerous misrouted ectopic mossy fibers. More relevantly, we describe how subgranular cells express PlexinD1 and how the absence of Sema3E induces a dysregulation of the proliferation of dentate gyrus progenitors leading to the presence of ectopic cells in the molecular layer. Lastly, Sema3E mutant mice displayed increased network excitability both in the dentate gyrus and the hippocampus proper

Sustained benefit of left ventricular remodelling after valve replacement for aortic stenosis

Background: Valve replacement for aortic stenosis (AS) determines negative ventricular remodelling. We used cross sectional and Doppler echocardiography to check how rapidly it occurs and to assess if these changes are sustained over time. Methods: We evaluated in 34 patients subjected to aortic valve replacement for AS morphological and functional (ejection fraction and E:A ratio) left ventricular data by echocardiography prior to surgery and 2 postoperative studies: early after surgery (pQ1) and at mid-term evolution (pQ2). Results: Left ventricular mass index was reduced at pQ1 (from 152 &#177; 47 g/m2 to 113 &#177; 31 g/m2; p < 0.01) as well as end-diastolic (from 51.3 mm to 48.3 mm; p < 0.03), end-systolic (from 32.2 mm to 29.4 mm; p < 0.02), interventricular septum (from 12.9 mm to 10.3 mm; p < 0.01), and posterior wall (from 12.5 mm to 11 mm; p < 0.01) dimensions. Left ventricular ejection fraction (from 61.2% to 65.2%; p < 0.04) and E:A ratio (from 0.94 to 0.98; p < 0.01) increased significantly at pQ1. There were no significant differences in measurements between pQ1 and pQ2. Conclusions: Aortic valve replacement surgery leads to a rapid negative left ventricular remodelling during the first 7 months, including a decrease in myocardial hypertrophy and an improvement in systolic and diastolic function. These beneficial hemodynamic changes are sustained for at least 3 years