Bryo-Activities: A Review on How Bryophytes Are Contributing to the Arsenal of Natural Bioactive Compounds against Fungi

Usually regarded as less evolved than their more recently diverged vascular sisters, which currently dominate vegetation landscape, bryophytes seem having nothing to envy to the defensive arsenal of other plants, since they had acquired a suite of chemical traits that allowed them to adapt and persist on land. In fact, these closest modern relatives of the ancestors to the earliest terrestrial plants proved to be marvelous chemists, as they traditionally were a popular remedy among tribal people all over the world, that exploit their pharmacological properties to cure the most different diseases. The phytochemistry of bryophytes exhibits a stunning assortment of biologically active compounds such as lipids, proteins, steroids, organic acids, alcohols, aliphatic and aromatic compounds, polyphenols, terpenoids, acetogenins and phenylquinones, thus it is not surprising that substances obtained from various species belonging to such ancestral plants are widely employed as antitumor, antipyretic, insecticidal and antimicrobial. This review explores in particular the antifungal potential of the three Bryophyta divisions\u2014mosses (Musci), hornworts (Anthocerotae) and liverworts (Hepaticae)\u2014to be used as a sources of interesting bioactive constituents for both pharmaceutical and agricultural areas, providing an updated overview of the latest relevant insights

The Low End of the Initial Mass Function in Young LMC Clusters: I. The Case of R136

We report the result of a study in which we have used very deep broadband V and I WFPC2 images of the R136 cluster in the Large Magellanic Cloud from the HST archive, to sample the luminosity function below the detection limit of 2.8 Mo previously reached. In these new deeper images, we detect stars down to a limiting magnitude of m_F555W = 24.7 (~ 1 magnitude deeper than previous works), and identify a population of red stars evenly distributed in the surrounding of the R136 cluster. A comparison of our color-magnitude diagram with recentely computed evolutionary tracks indicates that these red objects are pre-main sequence stars in the mass range 0.6 - 3 Mo. We construct the initial mass function (IMF) in the 1.35 - 6.5 Mo range and find that, after correcting for incompleteness, the IMF shows a definite flattening below ~ 2 Mo. We discuss the implications of this result for the R136 cluster and for our understanding of starburst galaxies formation and evolution in general.Comment: 29 pages, 6 tables, 11 figures included + 3 external files, accepted for publication by Ap.

Serological evidence of Toscana virus infection in Portuguese patients.

SUMMARYToscana virus (TOSV) is an emergingPhlebovirusof growing interest as a human pathogen in the Mediterranean Basin. In Portugal, however, little is known about the prevalence of TOSV infection. The aim of this work was to perform a seroprevalence study in patients with requests for laboratory diagnosis of vector-borne viruses. A total of 538 patients with and without neurological signs from 2004 to 2008 were studied by in-house indirect immunofluorescence assay and commercial enzyme-linked immunosorbent assays. A prevalence of 4·2% for IgG antibodies was found in the group of patients with neurological signs. Five (3%) of these had recent infections. In the group with no neurological signs, the IgG prevalence was 1·3%. Two samples, belonging to two patients, were also confirmed with plaque reduction neutralization tests with the TOSV ISS. Phl.3 Italian strain. This work showed that TOSV is present and causing disease from north to south in Portugal. The probable circulation of different phlebovirus serotypes in Portugal emphasizes the need for further studies

Elevated soluble receptor for advanced glycation end product levels in patients with acute coronary syndrome and positive cardiac troponin I

Objectives High levels of soluble receptor for advanced glycation end products (sRAGE) have been shown to have an atheroprotective role; however, no data are available on this molecule in acute coronary syndromes (ACS). We evaluated sRAGE levels in patients with non-ST segment elevation ACS (NSTE-ACS) or with chronic stable angina. Methods We studied 265 patients, 190 of whom had NSTE-ACS and 75 had chronic stable angina. Results Plasma sRAGE values were comparable in the two groups (P= 0.19). However, in the patients with NSTEACS,sRAGE levels were significantly higher in patients with cardiac troponin-I (cTnI) of more than or equal to 0.04 lg/l compared with those with cTnI of less than 0.04 lg/l [758 pg/ml (493-1536 pg/ml) vs. 454 pg/ml (167-899 pg/ml); P = 0.0037]. A significant correlation(r= 0.323, P = 0.0045) was found between sRAGE and cTnI levels in patients with NSTE-ACS.Conclusion Plasma sRAGE levels are elevated in patients with NSTE-ACS with positive cTnI, suggesting that they could be related to myocardial cell damage

Epidemiologia ambientale ben temperata: etica, sociologia e storia in un progetto di citizen science

The article presents the research performed by an Italian multi-disciplinary team jointly with a group of citizen scientists. The research was part of the project CitieS-Health (Citizen Science for Urban Environment and Health) funded by the EU under the Horizon2020 Programme. Aria di Ricerca in Valle del Serchio , one of the project five pilot studies, was performed in eight municipalities of Valle del Serchio (Lucca, Tuscany) and addressed the prevalence of chronic kidney diseases in the context of potential industrial pollution on health and, in particular, the presence of heavy metals in the environment. Since long, health and environmental issues have been of concern for the local residents who over the years promoted many initiatives to ensure adequate monitoring and appropriate policy interventions. Inspired by the PNS (Post-Normal Science) approach, and in particular the idea of Extended Peer Community, the study addressed local concerns and expectations integrating a multiplicity of competences, knowledges and perspectives. Researchers from Social Sciences and Humanities – namely history, sociology and ethics – collaborated with others from the health sector – epidemiology in particular – and with citizens in all the phases of the work: from the definition of the research objective to the collection, analysis and dissemination of the research results, and the suggestion of policy recommendations

Measles and rubella in Italy, e-learning course for health care workers

Introduction. Since 2003, strategic plans for the elimination of measles and congenitalrubella have been adopted in the World Health Organization European Region. In Italy,a network of reference laboratories for measles and rubella (MoRoNet) has been recentlyimplemented to ensure high-quality laboratory investigation for the confirmation ofcases and outbreaks. Training among health care workers (HCWs) is one of the tasks ofMoRoNet and an e-learning course was produced to improve the knowledge on internationaland national elimination plans and laboratory surveillance for measles and rubella.Methods. The course, based on the problem based learning methodology, was offeredfree of charge. Data about all participants and those who completed the course havebeen collected and analysed.Results. 5822 participants enrolled and 3995 (69%) completed the course; comparisonbetween pre- and post-test shows a significant improvement in knowledge. The averagescore obtained from the satisfaction questionnaire is 4.5 out of 5.Discussion and conclusions. Course’s results are satisfactory, and data show a significantimprovement in knowledge among participants. Most of them were satisfied withcontent, learning methodology and platform. Moreover, this course represents one ofthe possible strategies to overcome resistance and mistrust about vaccinations amongHCWs

Phleboviruses detection in Phlebotomus perniciosus from a human leishmaniasis focus in South-West Madrid region, Spain

Phlebotomus-borne (PhB-) viruses are distributed in large areas of the Old World and are widespread throughout the Mediterranean basin, where recent investigations have indicated that virus diversity is higher than initially suspected. Some of these viruses are causes of meningitis, encephalitis and febrile illnesses. In order to monitor the viral presence and the infection rate of PhB-viruses in a recently identified and well characterized human zoonotic leishmaniasis focus in southwestern Madrid, Spain, a sand fly collection was carried out. Methods Sand fly insects were collected in four stations using CDC light traps during 2012–2013 summer seasons. Screening for Phlebovirus presence both via isolation on Vero cells and via polymerase chain reaction (PCR), using degenerated primers targeting a portion of the L segment, was performed. The serological identity and phylogenetic relationships on the three genomic segments of the viral isolates were carried out. Results Six viral isolates belonging to different serological complexes of the genus Phlebovirus were obtained from fifty pools on a total of 963 P. perniciosus (202 females). Phylogenetic analysis and serological assays allowed the identification of two isolates of Toscana virus (TOSV) B genotype, three isolates strongly related to Italian Arbia virus (ARBV), and one isolate of a novel putative Phlebovirus related to the recently characterized Arrabida virus in South Portugal, tentatively named Arrabida-like virus. Positive male sand fly pools suggested that transovarial or venereal transmission could occur under natural conditions. Conclusions Our findings highlighted the presence of different Phlebovirus species in the South-West area of the Madrid Autonomous Community where an outbreak of cutaneous and visceral human leishmaniasis has been recently described. The evidence of viral species never identified before in Spain, as ARBV and Arrabida-like virus, and TOSV B genotype focus stability was demonstrated. Environmental aspects such as climate change, growing urbanization, socio-economic development could have contributed to the genesis of this wide ecological niche of PhB-viruses and Leishmania spp. The potential role of vertebrates as reservoir for the phleboviruses identified and the possibility of Phleboviruses-Leishmania co-infection in the same sand fly should be assessed. Furthermore the PhB-viruses impact on human health should be implemented.This study was funded by EU grant FP7-261504 EDENext and is catalogued by the EDENext Steering Committee as EDENext432 (http://www.edenext.eu). The contents of this publication are the sole responsibility of the authors and do not necessarily reflect the views of the European Commission.S

Lung and Heart Diseases Are Better Predicted by Pack-Years than by Smoking Status or Duration of Smoking Cessation in HIV Patients

BACKGROUND: The objective of this study was to assess the relationship of pack-years smoking and time since smoking cessation with risk of lung and heart disease. METHODS: We investigated the history of lung and heart disease in 903 HIV-infected patients who had undergone thoracic computed tomography (CT) imaging stratified by smoking history. Multimorbidity lung and heart disease (MLHD) was defined as the presence of 65 2 clinical or subclinical lung abnormalities and at least one heart abnormality. RESULTS: Among 903 patients, 23.7% had never smoked, 28.7% were former smokers and 47.6% were current smokers. Spirometry indicated chronic obstructive pulmonary disease in 11.4% of patients and MLHD was present in 53.6%. Age, male sex, greater pack-years smoking history and smoking cessation less than 5 years earlier vs. more than 10 years earlier (OR 2.59, 95% CI 1.27-5.29, p = 0.009) were independently associated with CT detected subclinical lung and heart disease. Pack-years smoking history was more strongly associated with MLHD than smoking status (p<0.001). CONCLUSIONS: MLHD is common even among HIV-infected patients who never smoked and pack- years smoking history is a stronger predictor than current smoking status of MLHD. A detailed pack-years smoking history should be routinely obtained and smoking cessation strategies implemente