95 research outputs found
Retour d'expérience sur les bassins d'orage en réseau unitaire: une solution pour limiter les rejets de temps de pluie
International audienceLe dĂ©veloppement des systĂšmes d'assainissement s'appuie souvent sur des structures construites il y a plusieurs dĂ©cennies. Ces systĂšmes font face Ă une augmentation des niveaux d'exigence qui leur sont assignĂ©s. Dans le viseur de l'arrĂȘtĂ© de 2015 notamment, les performances par temps de pluie des systĂšmes de collecte. Un retour d'expĂ©riences sur les bassins d'orage menĂ© par le Cerema fait le point sur les interrogations et les solutions trouvĂ©es par les gestionnaires. CONTEXTE DE L'ETUDE En collaboration avec l'AFB, le GEMCEA, le MinistĂšre de la transition Ă©cologique et solidaire et les Agences de l'eau, le Cerema a rĂ©alisĂ© un vaste retour d'expĂ©riences sur les bassins d'orage implantĂ©s sur les rĂ©seaux d'assainissement unitaires. L'objet de ce retour d'expĂ©riences Ă©tait de voir, sur le terrain, comment les gestionnaires/exploitants avaient eu recours Ă cette technique pour optimiser le fonctionnement de leur systĂšme, notamment par temps de pluie, et de collecter des informations techniques et financiĂšres quant au fonctionnement, Ă la conception, au dimensionnement de ce type d'ouvrages. A travers une recherche bibliographique et de nombreux entretiens rĂ©alisĂ©s auprĂšs de 17 maĂźtres d'ouvrage/exploitants, ce travail a aussi permis indirectement de recueillir les tĂ©moignages des personnes interrogĂ©es quant aux enjeux et aux problĂ©matiques auxquelles les services peuvent ĂȘtre confrontĂ©s, aux difficultĂ©s techniques rencontrĂ©es, souvent liĂ©es Ă leur contexte local, et Ă la façon dont les collectivitĂ©s ont cherchĂ© Ă y remĂ©dier notamment par la construction de bassins d'orage. Le projet ayant dĂ©butĂ© au 2nd semestre 2013 et s'Ă©tant terminĂ© en 2018, nous avons ainsi pu ĂȘtre les tĂ©moins, un peu malgrĂ© nous, des interrogations des diffĂ©rents acteurs vis-Ă -vis des Ă©volutions de la rĂ©glementation en matiĂšre d'assainissement : autosurveillance, diagnostic permanent, choix du critĂšre de conformitĂ© du systĂšme de collecte, ⊠ainsi que des consĂ©quences des lois NOTRe 5 et MAPTAM 6 sur la rĂ©partition des compĂ©tences Ă court et moyen termes. Cette communication fait volontairement le choix de se focaliser sur les Ă©lĂ©ments recueillis qui concernent les enjeux et opportunitĂ©s de l'autosurveillance et du diagnostic permanent, les dĂ©marches mises en place par les gestionnaires pour optimiser le fonctionnement de leur systĂšme et rĂ©pondre aux nouveaux critĂšres de conformitĂ© fixĂ©s par l'arrĂȘtĂ© de 2015
Integrating landscape genomics and spatially explicit approaches to detect loci under selection in clinal populations
Uncovering the genetic basis of adaptation hinges on the ability to detect loci under selection. However, population genomics outlier approaches to detect selected loci may be inappropriate for clinal populations or those with unclear population structure because they require that individuals be clustered into populations. An alternate approach, landscape genomics, uses individual-based approaches to detect loci under selection and reveal potential environmental drivers of selection. We tested four landscape genomics methods on a simulated clinal population to determine their effectiveness at identifying a locus under varying selection strengths along an environmental gradient. We found all methods produced very low type I error rates across all selection strengths, but elevated type II error rates under âweakâ selection. We then applied these methods to an AFLP genome scan of an alpine plant, Campanula barbata, and identified five highly supported candidate loci associated with precipitation variables. These loci also showed spatial autocorrelation and cline patterns indicative of selection along a precipitation gradient. Our results suggest that landscape genomics in combination with other spatial analyses provides a powerful approach for identifying loci potentially under selection and explaining spatially complex interactions between species and their environment
Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4.
The mucosa of the female reproductive tract plays a pivotal role in host defence. Pregnancy must alter immunological mechanisms at this interface to protect the conceptus. We sought to determine how estradiol (E2) alters the immune-responsiveness of cervical epithelial cells to ligand stimulation of Toll-like receptor (TLR)-2 and -4. Human ectocervical epithelial cells (HECECs) were cultured and co-incubated with two concentrations of E2 and peptidoglycan (PGN) or lipopolysaccharide (LPS) over durations that ranged between 10 minutes and 18 hours. Cytometric Bead Array was performed to quantify eight cytokines in the supernatant fluid. In response to PGN, HECECs co-incubated with E2 released lesser quantities of IL-1Ă and IFNÎł, higher levels of RANTES, and variable levels of IL-6 and IL-8 than those not exposed to E2. In contrast, HECECs co-incubated with LPS and E2 secreted increased levels of IL-1Ă, IL-6, IL-8, and IFNÎł at 2 and 18 hours than HECECs not exposed to E2, and reduced levels of RANTES at same study time-points. Estradiol alters the immune-responsiveness of cultured HECECs to TLR2 and TLR4 ligands in a complex fashion that appears to vary with bacterial ligand, TLR subtype, and duration of exposure. Our observations are consistent with the functional complexity that this mucosal interface requires for its immunological roles
Seasonality of Leaf and Fig Production in Ficus squamosa, a Fig Tree with Seeds Dispersed by Water
The phenology of plants reflects selection generated by seasonal climatic factors and interactions with other plants and animals, within constraints imposed by their phylogenetic history. Fig trees (Ficus) need to produce figs year-round to support their short-lived fig wasp pollinators, but this requirement is partially de-coupled in dioecious species, where female trees only develop seeds, not pollinator offspring. This allows female trees to concentrate seed production at more favorable times of the year. Ficus squamosa is a riparian species whose dispersal is mainly by water, rather than animals. Seeds can float and travel in long distances. We recorded the leaf and reproductive phenology of 174 individuals for three years in Chiang Mai, Northern Thailand. New leaves were produced throughout the year. Fig production occurred year-round, but with large seasonal variations that correlated with temperature and rainfall. Female and male trees initiated maximal fig crops at different times, with production in female trees confined mainly to the rainy season and male figs concentrating fig production in the preceding months, but also often bearing figs continually. Ficus squamosa concentrates seed production by female plants at times when water levels are high, favouring dispersal by water, and asynchronous flowering within male trees allow fig wasps to cycle there, providing them with potential benefits by maintaining pollinators for times when female figs become available to pollinate
Local flexibility in feeding behaviour and contrasting microhabitat use of an omnivore across latitudes
As the environment is getting warmer and species are redistributed, consumers can be forced to adjust their interactions with available prey, and this could have cascading effects within food webs. To better understand the capacity for foraging flexibility, our study aimed to determine the diet variability of an ectotherm omnivore inhabiting kelp forests, the sea urchin Echinus esculentus, along its entire latitudinal distribution in the northeast Atlantic. Using a combination of gut content and stable isotope analyses, we determined the diet and trophic position of sea urchins at sites in Portugal (42° N), France (49° N), southern Norway (63° N), and northern Norway (70° N), and related these results to the local abundance and distribution of putative food items. With mean estimated trophic levels ranging from 2.4 to 4.6, omnivory and diet varied substantially within and between sites but not across latitudes. Diet composition generally reflected prey availability within epiphyte or understorey assemblages, with local affinities demonstrating that the sea urchin adjusts its foraging to match the small-scale distribution of food items. A net âpreferenceâ for epiphytic food sources was found in northern Norway, where understorey food was limited compared to other regions. We conclude that diet change may occur in response to food source redistribution at multiple spatial scales (microhabitats, sites, regions). Across these scales, the way that key consumers alter their foraging in response to food availability can have important implication for food web dynamics and ecosystem functions along current and future environmental gradients
Pathogen-Mediated Proteolysis of the Cell Death Regulator RIPK1 and the Host Defense Modulator RIPK2 in Human Aortic Endothelial Cells
Porphyromonas gingivalis is the primary etiologic agent of periodontal disease that is associated with other human chronic inflammatory diseases, including atherosclerosis. The ability of P. gingivalis to invade and persist within human aortic endothelial cells (HAEC) has been postulated to contribute to a low to moderate chronic state of inflammation, although how this is specifically achieved has not been well defined. In this study, we demonstrate that P. gingivalis infection of HAEC resulted in the rapid cleavage of receptor interacting protein 1 (RIPK1), a mediator of tumor necrosis factor (TNF) receptor-1 (TNF-R1)-induced cell activation or death, and RIPK2, a key mediator of both innate immune signaling and adaptive immunity. The cleavage of RIPK1 or RIPK2 was not observed in cells treated with apoptotic stimuli, or cells stimulated with agonists to TNF-R1, nucleotide oligomerization domain receptor 1(NOD1), NOD2, Toll-like receptor 2 (TLR2) or TLR4. P. gingivalis-induced cleavage of RIPK1 and RIPK2 was inhibited in the presence of a lysine-specific gingipain (Kgp) inhibitor. RIPK1 and RIPK2 cleavage was not observed in HAEC treated with an isogenic mutant deficient in the lysine-specific gingipain, confirming a role for Kgp in the cleavage of RIPK1 and RIPK2. Similar proteolysis of poly (ADP-ribose) polymerase (PARP) was observed. We also demonstrated direct proteolysis of RIPK2 by P. gingivalis in a cell-free system which was abrogated in the presence of a Kgp-specific protease inhibitor. Our studies thus reveal an important role for pathogen-mediated modification of cellular kinases as a potential strategy for bacterial persistence within target host cells, which is associated with low-grade chronic inflammation, a hallmark of pathogen-mediated chronic inflammatory disorders
Global urban environmental change drives adaptation in white clover.
Urbanization transforms environments in ways that alter biological evolution. We examined whether urban environmental change drives parallel evolution by sampling 110,019 white clover plants from 6169 populations in 160 cities globally. Plants were assayed for a Mendelian antiherbivore defense that also affects tolerance to abiotic stressors. Urban-rural gradients were associated with the evolution of clines in defense in 47% of cities throughout the world. Variation in the strength of clines was explained by environmental changes in drought stress and vegetation cover that varied among cities. Sequencing 2074 genomes from 26 cities revealed that the evolution of urban-rural clines was best explained by adaptive evolution, but the degree of parallel adaptation varied among cities. Our results demonstrate that urbanization leads to adaptation at a global scale
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