Editorial Introduction: 'Encountering Australia, Confronting Catastrophe'

'Encountering Australia, Confronting Catastrophe'. These articles are based on papers presented at one of two consecutive conferences on Australia held in Europe during the northern summer of 2014. The European Association for Studies on Australia held a conference at the Monash University Prato Centre, Italy, in September that explored sites of contact, connection and exchange between Australia and the world, with particular emphasis on Europe, and with the aim of highlighting the importance of cross cultural dialogue. The conference drew on diverse modes and ideas of encountering Australia- imaginatively, theoretically, institutionally, politically, socially, historically, pedagogically and symbolically. Themed sessions included ‘Ecocultural Encounters' and ‘Ecopoetics', but animated discussion of our natural-cultural environment, whether in terms of climate change, migration and mobility, histories of war and violence, or national and collective memory, was evident across the varied foci. Tellingly, this conference was preceded by a symposium in the same month, hosted by the Australian Studies Centre at the University of Copenhagen, on ‘Cultural Response to Environmental Disaster in Australia.'

A transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in mice

Background: Fish currently supplies only 40% of the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) required to allow all individuals globally to meet the minimum intake recommendation of 500 mg/d. Therefore, alternative sustainable sources are needed. Objective: The main objective was to investigate the ability of genetically engineered Camelina sativa (20% EPA) oil (CO) to enrich tissue EPA and DHA relative to an EPA-rich fish oil (FO) in mammals. Methods: Six-week-old male C57BL/6J mice were fed for 10 wk either a palm oil–containing control (C) diet or diets supplemented with EPA-CO or FO, with the C, low-EPA CO (COL), high-EPA CO (COH), low-EPA FO (FOL), and high-EPA FO (FOH) diets providing 0, 0.4, 3.4, 0.3, and 2.9 g EPA/kg diet, respectively. Liver, muscle, and brain were collected for fatty acid analysis, and blood glucose and serum lipids were quantified. The expression of selected hepatic genes involved in EPA and DHA biosynthesis and in modulating their cellular impact was determined. Results: The oils were well tolerated, with significantly greater weight gain in the COH and FOH groups relative to the C group (P < 0.001). Significantly lower (36–38%) blood glucose concentrations were evident in the FOH and COH mice relative to C mice (P < 0.01). Hepatic EPA concentrations were higher in all EPA groups relative to the C group (P < 0.001), with concentrations of 0.0, 0.4, 2.9, 0.2, and 3.6 g/100 g liver total lipids in the C, COL, COH, FOL, and FOH groups, respectively. Comparable dose-independent enrichments of liver DHA were observed in mice fed CO and FO diets (P < 0.001). Relative to the C group, lower fatty acid desaturase 1 (Fads1) expression (P < 0.005) was observed in the COH and FOH groups. Higher fatty acid desaturase 2 (Fads2), peroxisome proliferator–activated receptor α (Ppara), and peroxisome proliferator–activated receptor γ (Pparg) (P < 0.005) expressions were induced by CO. No impact of treatment on liver X receptor α (Lxra) or sterol regulatory element-binding protein 1c (Srebp1c) was evident. Conclusions: Oil from transgenic Camelina is a bioavailable source of EPA in mice. These data provide support for the future assessment of this oil in a human feeding trial

Melt growth and microstructural control of single crystal YBCO high temperature superconducting materials

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 1997.Includes bibliographical references (leaves 254-263).by Karina E.A. Rigby.Ph.D

Facilitated Peptide Transport via the Mucosal Epithelium

A hallmark of autoimmunity is the breakdown of tolerance and generation of effector responses against self-antigens. Re-establishment of tolerance in autoimmune disorders was always the most desired treatment option; however, despite many efforts, clinical trials have been largely unsuccessful. This also applies to the generation of oral tolerance, which seems to be a default response type of the mucosa-associated lymphoid tissues to harmless antigens. In this study, we report improved efficacy of oral tolerance induction by coupling antigen with the newly identified mucosal carrier peptide 13C. Antigen coupled to 13C is efficiently taken up in the gastrointestinal tract and could be visualized in cells of the lamina propria. Oral, rectal, or nasal treatment effectively induced the proliferation of antigen-specific T cells with some increase in the frequency of regulatory T cells. In a model of delayed-type hypersensitivity, especially intrarectal tolerization treatment resulted in reduced footpad swelling, demonstrating a moderate tolerogenic effect of mucosal treatment with 13C coupled antigen. Coupling of antigens to a transmucosal carrier, therefore, is a promising tool to improve the efficacy of vaccination via mucosal surfaces

Neutral gas properties of Lyman continuum emitting galaxies:Column densities and covering fractions from UV absorption lines

The processes allowing the escape of ionizing photons from galaxies into the intergalactic medium are poorly known. To understand how Lyman continuum (LyC) photons escape galaxies, we constrain the HI covering fractions and column densities using ultraviolet HI and metal absorption lines of 18 star-forming galaxies which have Lyman series observations. Nine of these galaxies are confirmed LyC emitters. We fit the stellar continuum, dust attenuation, metal, and HI properties to consistently determine the UV attenuation, as well as the column densities and covering factors of neutral hydrogen and metals. We use synthetic interstellar absorption lines to explore the systematics of our measurements. Then we apply our method to the observed UV spectra of low-redshift and z-2 galaxies. The observed HI lines are found to be saturated in all galaxies. An indirect approach using OI column densities and the observed O/H abundances yields HI column densities of 18.6 to 20 cm-2. These columns are too high to allow the escape of ionizing photons. We find that the known LyC leakers have HI covering fractions less than unity. Ionizing photons escape through optically thin holes/channels in a clumpy interstellar medium. Our simulations confirm that the HI covering fractions are accurately recovered. The SiII and HI covering fractions scale linearly, in agreement with observations from stacked Lyman break galaxy spectra at z-3. Thus, with an empirical correction, the SiII absorption lines can also be used to determine the HI coverage. Finally, we show that a consistent fitting of dust attenuation, continuum and absorption lines is required to properly infer the covering fraction of neutral gas and subsequently to infer the escape fraction of ionizing radiation. These measurements can estimate the LyC escape fraction, as we demonstrate in a companion paper

Differential effects of EPA vs. DHA on postprandial vascular function and the plasma oxylipin profile in men

Our objective was to investigate the impact of EPA versus DHA, on arterial stiffness and reactivity, and underlying mechanisms (with a focus on plasma oxylipins), in the postprandial state. In a 3-arm cross-over acute test meal trial men (n=26, 35-55y) at increased CVD risk, received a high fat (42.4g) test meal providing 4.16 g of EPA or DHA or control oil in random order. At 0 h and 4 h, blood samples were collected to quantify plasma fatty acids, LCn-3PUFAs derived oxylipins, nitrite and hydrogen sulfide and serum lipids and glucose. Vascular function was assessed using blood pressure, Reactive Hyperaemia Index (RHI), Pulse Wave Velocity and Augmentation Index (AIx). The DHA-rich oil significantly reduced AIx by 13% (P=0.047) with the decrease following EPA-rich oil intervention not reaching statistical significance. Both interventions increased EPA and DHA derived oxylipins in the acute postprandial state, with an (1.3 fold) increase in 19,20-DiHDPA evident after DHA intervention (P < 0.001). In conclusion, a single dose of DHA significantly improved postprandial arterial stiffness as assessed by AIx, which if sustained would be associated with a significant decrease in CVD risk. The observed increases in oxylipins provide a mechanistic insight for the AIx effect

Anthocyanins do not influence long-chain n-3 fatty acid status:Studies in cells, rodents and humans

Increased tissue status of the long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) is associated with cardiovascular and cognitive benefits. Limited epidemiological and animal data suggest that flavonoids, and specifically anthocyanins, may increase EPA and DHA levels, potentially by increasing their synthesis from the shorter-chain n-3 PUFA, α-linolenic acid. Using complimentary cell, rodent and human studies we investigated the impact of anthocyanins and anthocyanin-rich foods/extracts on plasma and tissue EPA and DHA levels and on the expression of fatty acid desaturase 2 (FADS2), which represents the rate limiting enzymes in EPA and DHA synthesis. In experiment 1, rats were fed a standard diet containing either palm oil or rapeseed oil supplemented with pure anthocyanins for 8 weeks. Retrospective fatty acid analysis was conducted on plasma samples collected from a human randomized controlled trial where participants consumed an elderberry extract for 12 weeks (experiment 2). HepG2 cells were cultured with α-linolenic acid with or without select anthocyanins and their in vivo metabolites for 24 h and 48 h (experiment 3). The fatty acid composition of the cell membranes, plasma and liver tissues were analyzed by gas chromatography. Anthocyanins and anthocyanin-rich food intake had no significant impact on EPA or DHA status or FADS2 gene expression in any model system. These data indicate little impact of dietary anthocyanins on n-3 PUFA distribution and suggest that the increasingly recognized benefits of anthocyanins are unlikely to be the result of a beneficial impact on tissue fatty acid status