Mutations In RAD27 Define A Potential Link Between G₁ Cyclins And DNA Replication

The yeast Saccharomyces cerevisiae has three G1 cyclin (CLN) genes with overlapping functions. To analyze the functions of the various CLN genes, we examined mutations that result in lethality in conjunction with loss of cln1 and cln2. We have isolated alleles of RAD27/ERC11/YKL510, the yeast homolog of the gene encoding flap endonuclease 1, FEN-1.cln1 cln2 rad27/erc11 cells arrest in S phase; this cell cycle arrest is suppressed by the expression of CLN1 or CLN2 but not by that of CLN3 or the hyperactive CLN3-2. rad27/erc11 mutants are also defective in DNA damage repair, as determined by their increased sensitivity to a DNA-damaging agent, increased mitotic recombination rates, and increased spontaneous mutation rates. Unlike the block in cell cycle progression, these phenotypes are not suppressed by CLN1 or CLN2. CLN1 and CLN2 may activate an RAD27/ERC11-independent pathway specific for DNA synthesis that CLN3 is incapable of activating. Alternatively, CLN1 and CLN2 may be capable of overriding a checkpoint response which otherwise causes cln1 cln2 rad27/erc11 cells to arrest. These results imply that CLN1 and CLN2 have a role in the regulation of DNA replication. Consistent with this, GAL-CLN1 expression in checkpoint-deficient, mec1-1 mutant cells results in both cell death and increased chromosome loss among survivors, suggesting that CLN1 overexpression either activates defective DNA replication or leads to insensitivity to DNA damage

A Comparative Study of Attitudes of First Grade Children in Two Reading Programs--Individualized and Basal

Many studies have been done comparing individualized and basal reading programs, but few have employed a valid attitude scale to evaluate attitudes toward reading at the primary level

introduction tensions of empire: colonial control and visions of rule

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136470/1/ae.1989.16.4.02a00010.pd

Humanities Heritage 3D Visualization: Theory and Practice

The past thirty years have seen a tremendous upsurge in the use of digital modeling in archaeology as well as in projects and applications based in the humanities. Digital representations can create new forms of visual knowledge, which in turn can lead to new research streams. As such, digital models have become increasingly important tools in a wide range of applications. Despite the power of these models as representational and visualization tools, the tools themselves are often difficult to use and not easily accessible by the novice. This proposal is for a 'digital toolbox' that bridges this gap. A unique feature of this institute is the incorporation of visits to Arkansas State University heritage sites modeled by the University's Center for Digital Initiatives. Beyond this, in bringing together an impressive group of lecturers working in the digital humanities, the institute itself would create an important resource in the form of a community of scholars, encouraging future collaborations between individuals and universities

Aerogel-Based Antennas for Aerospace and Terrestrial Applications

Systems and methods for lightweight, customizable antenna with improved performance and mechanical properties are disclosed. In some aspects, aerogels can be used, for example, as a substrate for antenna fabrication. The reduced weight and expense, as well as the increased ability to adapt antenna designs, permits a systems to mitigate a variety of burdens associated with antennas while providing added benefits

Potential flow artifacts associated with benthic experimental gear: Deep-sea mudbox examples

In response to the growing recognition of the potential effects of near-bed hydrodynamics on various benthic processes, flume studies were conducted to document fine-scale flow patterns over several types of mudboxes that have been used to study colonization by deep-sea organisms. Mudboxes are typically filled with natural sediments or sediment treatments and placed in the field to observe how timing, larval supply and sediment composition may affect larval settlement. This study addresses potential hydrodynamic biases of mudbox structures as obstructions to the near-bed flow. Detailed velocity profiles were made over two types of “free vehicle” mudboxes that could be deployed and recovered from a surface vessel. One of these (“Old Free Vehicle”) was not designed with regard for potential hydrodynamic biases whereas the other (“New Free Vehicle”) was designed specifically to minimize flow disturbances and maintain a realistic boundary-layer flow over the mudbox sediments. Flume velocity profiles also were made over two smaller mudboxes designed to be deployed by a submersible, one (“Flush Sediment Tray”) which was designed to be placed flush with the ocean bottom, thus minimizing flow disturbance, and another (“Raised Mudbox”) which was not. Flume simulations indicated that the Old Free Vehicle and the Raised Mudbox cause considerable disturbance to the near-bed flow regime; flows over the mudbox sediment surface differed markedly from those predicted for the natural seabed and those observed over the flume bed in the absence of the mudboxes. Flow accelerations, growing secondary boundary layers and eddy formation were observed over these mudbox sediments, and vertical velocity profiles varied considerably in the along-channel direction. The alternative mudbox designs (New Free Vehicle and Flush Sediment Tray) were largely successful in reducing or eliminating these flow artifacts. Boundary-layer flows over both the New Free Vehicle and the Flush Sediment Tray were much more uniform, and velocity profiles over the sediment surfaces were very similar to those in the empty flume channel and those predicted for a natural deep-sea habitat. In addition, there was no evidence of eddy formation and other major flow disturbances. These flume studies underscore the benefit of considering potential hydrodynamic effects in designing benthic experimental sampling gear to reduce potential flow disturbances that may bias data collections and confound data interpretation

Multisystem reactions during egg oral food challenges may be associated with less severe reactions on initial presentation

In this study, we assessed whether multisystem reactions to egg and extensively-heated (EH) egg during OFCs were associated with a history of multisystem reactions. Records of children, who underwent OFC to egg or EH egg over a five-year period were reviewed. Of the 120 challenges, 26 (21.67 %) failed, with 38.4 % (10/26) having multisystem reactions. Of the 13 who had multisystem reactions on initial presentation, only two (15.4 %) had a similar OFC outcome. Eighty percent (8/10) of those who had a multisystem OFC reaction had a less severe initial presentation. Initial and OFC multisystem reactions were not associated with each other

Flight nurse turnover: where they go, why, and what they do

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29462/1/0000545.pd

The Role of Experiential Value from Product Presentation on Consumer Responses towards an Apparel Website

Offering enhanced visual images of apparel products on a website, such as alternative views, image enlargement, and views of a product on a model, is important because product presentation has been found to facilitate positive consumer responses resulting from both hedonic or utilitarian value