8 research outputs found
Epithelial expression of extracellular matrix metalloproteinase inducer/CD147 and matrix metalloproteinase-2 in neoplasms and precursor lesions derived from cutaneous squamous cells: An immunohistochemical study
WOS: 000326996100003PubMed: 23948694Extracellular matrix metalloproteinase inducer (CD147) is a transmembrane glycoprotein involved in the regulation of matrix metalloproteinases (MMPs). The study investigated CD147 and MMP-2 expression in epidermis of cutaneous squamous lesions. CD147 and MMP-2 expressions were evaluated immunohistochemically in 44 specimens: 18 actinic keratoses (AK), 6 squamous cell carcinomas in situ (SCCIS), 13 squamous cell carcinomas (SCC; peritumoral and invasive portions assessed), and 7 normal skins. Patterns of expression were assessed, with MMP-2 in nuclei (MMP-2n) and cytoplasm (MMP-2c) evaluated separately. The expression of each marker was quantified using a calculated immunohistochemical/histologic score (H-score). Correlations were analyzed for the marker H-scores in each study group. Associations between H-scores and histopathologic parameters were also evaluated. CD147 H-score was the highest in SCC (invasive islands), followed by AK, SCCIS, and control specimens, respectively. MMP-2n and MMP-2c H-scores were the highest in AK, followed by SCCIS, SCC, and control specimens, respectively. MMP-2c and MMP-2n H-scores were significantly higher in peritumoral epidermis than in invasive islands of SCC. MMP-2c and CD147 H-scores were positively correlated in the peritumoral SCCs. CD147 H-score was positively correlated with tumor differentiation in SCC. The findings suggest that overexpression of CD147 plays a role in the development of SCC. (C) 2013 Elsevier GmbH. All rights reserved.Kirikkale University Scientific Research CommitteeKirikkale UniversityThe project was supported by Kirikkale University Scientific Research Committee
Polyclonal aptamer libraries as binding entities on a graphene FET based biosensor for the discrimination of apo- and holo- retinol binding protein 4
Oligonucleotide DNA aptamers represent an emergently important class of binding entities towards as different analytes as small molecules or even whole cells. Without the canonical isolation of individual aptamers following the SELEX process already the focused polyclonal libraries prepared by this in vitro evolution and selection can directly be used to label their dedicated analytes and to serve as binding molecules on surfaces. Here we report the first instance of a sensor able to discriminate between loaded and unloaded retinol binding protein 4 (RBP4), an important biomarker for the prediction of diabetes and kidney disease. The sensor relies purely on two aptamer libraries tuned such, that they discriminate between the protein isoforms, requiring no further sample labelling to detect RBP4 in both state. The evolution, binding properties of the libraries and the functionalization of graphene FET sensor chips are presented as well as the functionality of the resulting biosensor
Activity of Single Insect Olfactory Receptors Triggered by Airborne Compounds Recorded in Self-Assembled Tethered Lipid Bilayer Nanoarchitectures
Membrane proteins are among the most difficult to study
as they
are embedded in the cellular membrane, a complex and fragile environment
with limited experimental accessibility. To study membrane proteins
outside of these environments, model systems are required that replicate
the fundamental properties of the cellular membrane without its complexity.
We show here a self-assembled lipid bilayer nanoarchitecture on a
solid support that is stable for several days at room temperature
and allows the measurement of insect olfactory receptors at the single-channel
level. Using an odorant binding protein, we capture airborne ligands
and transfer them to an olfactory receptor from Drosophila
melanogaster (OR22a) complex embedded in the lipid
membrane, reproducing the complete olfaction process in which a ligand
is captured from air and transported across an aqueous reservoir by
an odorant binding protein and finally triggers a ligand-gated ion
channel embedded in a lipid bilayer, providing direct evidence for
ligand capture and olfactory receptor triggering facilitated by odorant
binding proteins. This model system presents a significantly more
user-friendly and robust platform to exploit the extraordinary sensitivity
of insect olfaction for biosensing. At the same time, the platform
offers a new opportunity for label-free studies of the olfactory signaling
pathways of insects, which still have many unanswered questions
Development of an electro-mechanic control system for seed-metering unit of single seed corn planters Part I: Design and laboratory simulation
Characteristics of food allergy in children: National multicenter study
Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI) -- JUN 01-05, 2019 -- Lisbon, PORTUGALWOS: 000480254003261European Acad Allergy & Clin Immuno