The Mechanical Development of the Piccolo

The purpose of this document is to explore the mechanical development of the piccolo in relation to the flute. Although there have been several studies conducted about the mechanical development of the flute, the piccolo has never received this special attention and deserves to have its story told. The piccolo has gained prominence as an integral part of the orchestra and has also become a popular solo instrument. Mechanically, the piccolo has developed slowly in relation to the flute from a one-key instrument to the modern Boehm system in use today.The first step in this project began with an investigation of the writings that already exist on this subject, such as dictionaries, encyclopedias, books, and dissertations. Next, interviews were conducted with museum curators, instrument makers, and performers, and historical instrument collections throughout the world were examined. After careful review of these materials, a complete mechanical development of the piccolo has been developed.Each chapter of this document examines the mechanical improvements in terms of the tube and materials, the headjoint, and the mechanism during each stylistic period. With careful examination of the physical properties of each instrument, the reasons for these changes will become clear. The following chapters will include pertinent information regarding the similarities and differences between the piccolo and the flute, the role and importance of families of flutemakers, as well as shed light on reasons why composers became interested in scoring for these small flutes

Rural, Cultural & Global Health: As Seen Through the Eyes of Doctoral Students at the University of New Mexico

When our early UNM College of Nursing PhD students wrote Volumes 1-3 of this compilation a decade ago, our program was new and growing, and most of the students lived here in New Mexico or in neighboring states. Much has changed since the initial volumes emerged from our course entitled Rural and Cultural Health (Nursing 611), a graduate elective. The world has embraced enormous linkages through technology. Refugee and migration patterns the world over have more people displaced and seeking a permanent home than ever before. International, national, regional and local contexts, conflicts, politics, health-related policies, economic struggles, racial/ethnic diversity, other kinds of human diversity, and all dimensions of health care have undergone tremendous change and continue to do so at a rapid pace. Students from our own and other universities now take this course online and contribute their insights and experience. Nursing 611 has evolved into an 8-week summer elective offered in even-numbered years, focused on three components of health care: global health, cultural health, and rural health. The students, who represent diversity in heritage, geographic location, and clinical/research interests, were asked to produce 3 briefs, or short papers (each 3-4 pages long), on the three components (global, cultural, and rural health). They were encouraged to write in an engaging style, even resembling the tone of a Ted Talk, to interest readers. This collection represents their focused writing in the three areas. We believe that the state of New Mexico holds unique cultures, characteristics, and problems, yet the health disparities, inequities, population trends, migration patterns, and general state of rural/cultural health the world over are more similar than different. Whatever your own disciplinary connections, we invite you to explore the critical issues presented in the following collection of short essays. In this class, we have defined both culture and rurality broadly and in multiple contexts. Much remains to be done, both locally and globally, to improve the health status of our varied populations and communities. Please join us in the analysis and resolution of the health challenges, inequities, and unresolved needs that characterize many rural and cultural settings.https://digitalrepository.unm.edu/rural-cultural-health/1005/thumbnail.jp

Observation of vanishing charge dispersion of a nearly-open superconducting island

Isolation from the environment determines the extent to which charge is confined on an island, which manifests as Coulomb oscillations such as charge dispersion. We investigate the charge dispersion of a nanowire transmon hosting a quantum dot in the junction. We observe rapid suppression of the charge dispersion with increasing junction transparency, consistent with the predicted scaling law which incorporates two branches of the Josephson potential. We find improved qubit coherence times at the point of highest suppression, suggesting novel approaches for building charge-insensitive qubits

The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells

An in-depth proteomic comparison of human-induced pluripotent stem cells, and their parent fibroblast cells, with embryonic stem cells shows that the reprogramming process comprehensively remodels protein expression levels, creating cells that closely resemble natural stem cells

Exhaustive search for epistatic effects on the human methylome

Studies assessing the existence and magnitude of epistatic effects on complex human traits provide inconclusive results. The study of such effects is complicated by considerable increase in computational burden, model complexity, and model uncertainty, which in concert decrease model stability. An additional source introducing significant uncertainty with regard to the detection of robust epistasis is the biological distance between the genetic variation and the trait under study. Here we studied CpG methylation, a genetically complex molecular trait that is particularly close to genomic variation, and performed an exhaustive search for two-locus epistatic effects on the CpG-methylation signal in two cohorts of healthy young subjects. We detected robust epistatic effects for a small number of CpGs (N = 404). Our results indicate that epistatic effects explain only a minor part of variation in DNA-CpG methylation. Interestingly, these CpGs were more likely to be associated with gene-expression of nearby genes, as also shown by their overrepresentation in DNase I hypersensitivity sites and underrepresentation in CpG islands. Finally, gene ontology analysis showed a significant enrichment of these CpGs in pathways related to HPV-infection and cancer

Common epigenetic variation in a European population of mentally healthy young adults

DNA methylation represents an important link between structural genetic variation and complex phenotypes. The study of genome-wide CpG methylation and its relation to traits relevant to psychiatry has become increasingly important. Here, we analyzed quality metrics of 394,043 CpG sites in two samples of 568 and 319 mentally healthy young adults. For 25% of all CpGs we observed medium to large common epigenetic variation. These CpGs were overrepresented in open sea and shore regions, as well as in intergenic regions. They also showed a strong enrichment of significant hits in association analyses. Furthermore, a significant proportion of common DNA methylation is at least partially genetically driven and thus may be observed similarly across tissues. These findings could be of particular relevance for studies of complex neuropsychiatric traits, which often rely on proxy tissues

Aproveitamento de um resíduo fibroso de soja para a produção de goma xantana em cultivo semi-sólido

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Ubiquitylation of the ER-Shaping Protein Lunapark via the CRL3KLHL12 Ubiquitin Ligase Complex

Summary: Cullin-RING ligases (CRLs) control key cellular processes by promoting ubiquitylation of a multitude of soluble cytosolic and nuclear proteins. Subsets of CRL complexes are recruited and activated locally at cellular membranes; however, few CRL functions and substrates at these distinct cellular compartments are known. Here, we use a proteomic screen to identify proteins that are ubiquitylated at cellular membranes and found that Lunapark, an endoplasmic reticulum (ER)-shaping protein localized to ER three-way junctions, is ubiquitylated by the CRL3KLHL12 ubiquitin ligase. We demonstrate that Lunapark interacts with mechanistic target of rapamycin complex-1 (mTORC1), a central cellular regulator that coordinates growth and metabolism with environmental conditions. We show that mTORC1 binds Lunapark specifically at three-way junctions, and lysosomes, where mTORC1 is activated, make contact with three-way junctions where Lunapark resides. Inhibition of Lunapark ubiquitylation results in neurodevelopmental defects indicating that KLHL12-dependent ubiquitylation of Lunapark is required for normal growth and development