A bioinformatic analysis identifies circadian expression of splicing factors and time-dependent alternative splicing events in the HD-MY-Z cell line

The circadian clock regulates key cellular processes and its dysregulation is associated to several pathologies including cancer. Although the transcriptional regulation of gene expression by the clock machinery is well described, the role of the clock in the regulation of post-transcriptional processes, including splicing, remains poorly understood. In the present work, we investigated the putative interplay between the circadian clock and splicing in a cancer context. For this, we applied a computational pipeline to identify oscillating genes and alternatively spliced transcripts in time-course high-throughput data sets from normal cells and tissues, and cancer cell lines. We investigated the temporal phenotype of clock-controlled genes and splicing factors, and evaluated their impact in alternative splice patterns in the Hodgkin Lymphoma cell line HD-MY-Z. Our data points to a connection between clock-controlled genes and splicing factors, which correlates with temporal alternative splicing in several genes in the HD-MY-Z cell line. These include the genes DPYD, SS18, VIPR1 and IRF4, involved in metabolism, cell cycle, apoptosis and proliferation. Our results highlight a role for the clock as a temporal regulator of alternative splicing, which may impact malignancy in this cellular model

Real wave propagation in the isotropic relaxed micromorphic model

For the recently introduced isotropic relaxed micromorphic generalized continuum model, we show that under the assumption of positive definite energy, planar harmonic waves have real velocity. We also obtain a necessary and sufficient condition for real wave velocity which is weaker than positive-definiteness of the energy. Connections to isotropic linear elasticity and micropolar elasticity are established. Notably, we show that strong ellipticity does not imply real wave velocity in micropolar elasticity, while it does in isotropic linear elasticity

Formulación del plan de manejo de residuos sólidos peligrosos de la Empresa Coats Cadena S.A.

La formulación del proyecto plan de gestión de residuos sólidos peligrosos generados en Coats Cadena S.A. se hace necesario para dar continuidad al manejo que la empresa da al aspecto ambiental y sus impactos en recursos naturales y a su vez, dar cumplimiento a los lineamientos ambientales vigentes. Un aspecto positivo de la mpresa Coats Cadena S.A. es que cuenta con un Programa de Gestión y Manejo Integral de Residuos Sólidos (PGIRS) elaborado en el año 1998, el cual constituye la herramienta base en el manejo de sus Residuos. Debido a la entrada en vigencia del Decreto 4741 de 2005 por el cual se reglamenta la prevención y manejo de residuos o desechos peligrosos, se hace necesario la actualización de dicho programa con los nuevos lineamientos exigidos. Dentro de la política de Medio Ambiente de Coats Cadena S.A. en el numeral 1 enuncia; “Asegurar que sus operaciones cumplan con todas las legislaciones existentes y los estándares ambientales definidos por el grupo”. Teniendo en cuenta lo anterior y el Decreto 4741, este proyecto incluirá lineamientos para: • Elaborar un plan de manejo de los residuos o desechos peligrosos que se generan en los diferentes procesos productivos. • Identificar las características de peligrosidad de cada uno de los residuos o desechos peligrosos que se generen. • Garantizar que el envasado o empacado, embalado y etiquetado de sus residuos o desechos peligrosos, se realice conforme a la normatividad vigente. NTC 1692. (Art. 10 Decreto 4741 de 2005). • Dar cumplimiento a lo establecido en el decreto 1609 de 2002 y suministrar al transportista de los residuos o desechos peligrosos las respectivas hojas de seguridad. • Atender cualquier accidente o eventualidad que se presente mediante un plan de emergencia. • Exigir y conservar la documentación de ley a cada uno de los receptores. • Garantizar la correcta contratación de servicios de almacenamiento, aprovechamiento, tratamiento y disposición final de los residuos y desechos generados, de conformidad con la normatividad ambiental vigente. Este proyecto se presenta en la modalidad de monografía de compilación de tipo investigativo

Alexina de Magalhães e Lavinia Raymond: mediações sobre cultura popular e folclore negro em dois tempos

This study discusses how widely-disseminated interpretations of racism and black folklore can be transformed via ethnographic research, which we understand as cultural mediation. Alexina de Magalhães Pinto and Lavinia Costa Raymond were two white intellectuals, professors, and authors who, although forgotten nowadays, projected themselves in the intellectual and academic worlds of their time. They were active during two decisive moments in debates on racial issues in Brazil: from 1900 to 1920 and from 1935 to 1955. In their own (although initial and ambiguous) ways, they each confronted ideas about the disappearance of Afro-Brazilian cultural expressions, instead focusing on evidence of the ways in which the descendants of the enslaved maintained their cultural heritage.Este artigo discute como teses interpretativas muito compartilhadas sobre o racismo e o folclore negro podem se transformar a partir do trabalho de pesquisa etnográfica, aqui entendido como uma prática de mediação cultural. Alexina de Magalhães Pinto e Lavinia Costa Raymond foram duas intelectuais brancas, professoras e autoras, que apesar de hoje esquecidas, projetaram-se no mundo letrado e acadêmico de seu tempo. Atuaram em dois momentos decisivos, mesmo que distintos, para os debates sobre a questão racial no Brasil: as décadas de 1900 a 1920 e de 1935 a 1955. Enfrentaram, cada uma a sua maneira, ainda que de forma inicial e ambígua, as teses sobre o desaparecimento das expressões culturais afro-brasileiras, encontrando caminhos para a valorização do patrimônio cultural construído pelos descendentes de escravizados

Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures

<p>Abstract</p> <p>Background</p> <p>Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of lingocellulosic materials is essential for the success of these processes.</p> <p>Results</p> <p>Conversion of arabinose and glucose to hydrogen, by extreme thermophilic, anaerobic, mixed cultures was studied in continuous (70°C, pH 5.5) and batch (70°C, pH 5.5 and pH 7) assays. Two expanded granular sludge bed (EGSB) reactors, R_araband R_gluc, were continuously fed with arabinose and glucose, respectively. No significant differences in reactor performance were observed for arabinose and glucose organic loading rates (OLR) ranging from 4.3 to 7.1 kgCOD m^-3d^-1. However, for an OLR of 14.2 kgCOD m^-3d^-1, hydrogen production rate and hydrogen yield were higher in R_arabthan in R_gluc(average hydrogen production rate of 3.2 and 2.0 LH₂L^-1d^-1and hydrogen yield of 1.10 and 0.75 molH₂mol^-1_substratefor R_araband R_gluc, respectively). Lower hydrogen production in R_glucwas associated with higher lactate production. Denaturing gradient gel electrophoresis (DGGE) results revealed no significant difference on the bacterial community composition between operational periods and between the reactors. Increased hydrogen production was observed in batch experiments when hydrogen partial pressure was kept low, both with arabinose and glucose as substrate. Sugars were completely consumed and hydrogen production stimulated (62% higher) when pH 7 was used instead of pH 5.5.</p> <p>Conclusions</p> <p>Continuous hydrogen production rate from arabinose was significantly higher than from glucose, when higher organic loading rate was used. The effect of hydrogen partial pressure on hydrogen production from glucose in batch mode was related to the extent of sugar utilization and not to the efficiency of substrate conversion to hydrogen. Furthermore, at pH 7.0, sugars uptake, hydrogen production and yield were higher than at pH 5.5, with both arabinose and glucose as substrates.</p

A Bioinformatics Approach

By regulating the timing of cellular processes, the circadian clock provides a way to adapt physiology and behaviour to the geophysical time. In mammals, a light-entrainable master clock located in the suprachiasmatic nucleus (SCN) controls peripheral clocks that are present in virtually every body cell. Defective circadian timing is associated with several pathologies such as cancer and metabolic and sleep disorders. To better understand the circadian regulation of cellular processes, we developed a bioinformatics pipeline encompassing the analysis of high-throughput data sets and the exploitation of published knowledge by text-mining. We identified 118 novel potential clock- regulated genes and integrated them into an existing high-quality circadian network, generating the to-date most comprehensive network of circadian regulated genes (NCRG). To validate particular elements in our network, we assessed publicly available ChIP-seq data for BMAL1, REV-ERBα/β and RORα/γ proteins and found strong evidence for circadian regulation of Elavl1, Nme1, Dhx6, Med1 and Rbbp7 all of which are involved in the regulation of tumourigenesis. Furthermore, we identified Ncl and Ddx6, as targets of RORγ and REV-ERBα, β, respectively. Most interestingly, these genes were also reported to be involved in miRNA regulation; in particular, NCL regulates several miRNAs, all involved in cancer aggressiveness. Thus, NCL represents a novel potential link via which the circadian clock, and specifically RORγ, regulates the expression of miRNAs, with particular consequences in breast cancer progression. Our findings bring us one step forward towards a mechanistic understanding of mammalian circadian regulation, and provide further evidence of the influence of circadian deregulation in cancer

REDES NEURAIS APLICADAS À CLASSIFICAÇÃO DE TRÁFEGO DE REDES DE COMPUTADORES UTILIZANDO OS PROTOCOLOS TCP E UDP

This article presents the use of the Artificial Neural Network (ANN) on measurement and classification of traffic data using TCP and UDP transport protocols. I has been used the Adaline and Perceptron ANN to classify a data network traffic in the following categories: Source IP, destination IP, source port, destination port, type protocol (TCP or UDP and if the( connection started from the internal or external network. The implemented system allowed the traffic classification process can be done in a more dynamic way. Thus, the use of Artificial Neural Networks for classification and measurement of traffic on computer networks, using TCP and UDP transport protocols, was considered a productive way and applicable to the problem of classification of traffic data.Este artigo apresenta o uso da Rede Neural Artificial (RNA) na mensuração e classificação de dados trafegados utilizando os protocolos de transporte TCP e UDP. Foram utilizadas as RNAs Adaline e Perceptron  para classificar o tráfego de uma rede de dados nas seguintes categorias: IP de origem, IP de destino, porta de origem, porta de destino, tipo do protocolo (TCP ou UDP) e se a conexão partiu da rede interna ou externa. O sistema implementado permitiu que o processo  de classificação do tráfego seja feito de forma mais dinâmica. Assim, o uso de Redes Neurais Artificiais para a classificação e mensuração de tráfego de redes de computadores, utilizando protocolos de transporte TCP e UDP, foi considerado uma maneira produtiva e aplicável ao problema de classificação de dados trafegados