Generación de vínculos interinstitucionales y redes en el centro bonaerense a partir del proyecto de extensión “Promoción de la Agroecología, otro modelo de producción es posible”

La demanda social hacia sistemas sustentables de producción requiere de una articulación interinstitucional que permita dar respuestas en el diseño, transición y acompañamiento para su transformación. En este proceso las instituciones públicas participantes efectúan aportes desde la investigación, extensión y transferencia, en diálogo con las necesidades y prácticas de los propios productores y sus organizaciones, desde una perspectiva de trabajo colaborativa y participativa. Esta presentación se centra en compartir parte de los resultados de la ejecución del proyecto de extensión titulado: “Promoción de la Agroecología, otro modelo de producción es posible” desarrollado durante el período 2020-2021 en el contexto de pandemia Covid19.EEA BalcarceFil: Block, Karina Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina.Fil: Wynne, María Josefina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina.Fil: Menici, María Daniela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina.Fil: Zamora, Martín Sergio. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina.Fil: Vázquez, Patricia. Actividad Privada; ArgentinaFil: Iturralde Elortegui, María del Rosario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Olavarria; Argentina.Fil: Giaquinta, Alejandro Enzo. Ministerio de Desarrollo Agrario. Chacra Experimental Blanca Grande; Argentina.Fil: Farrando, Luis Adrián. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina.Fil: Martinefsky, María Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Olavarria; Argentina.Fil: Oyesqui, Lía Ana Eloisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Olavarria; Argentina.Fil: Andrich, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Olavarria; Argentina

Total phenolic content and antioxidant activity of different streams resulting from pilot-plant processes to obtain Amaranthus mantegazzianus protein concentrates

Antioxidant properties of different pilot-plant process streams to obtain Amaranthus mantegazzianus protein concentrates (APC) were evaluated. Conventional process (CP) (alkaline extraction and isoelectric precipitation) and two alternative processes (APs): (1) acid pre-treatment stage combined with isoelectric precipitation and (2) acid pre-treatment stage combined with ultrafiltration were applied at pilot-plant scale to obtain APC. Methanol and water extracts of APC and other fractions obtained in the processes were evaluated by Folin-Ciocalteau method in order to determine total phenolic content and by DPPH radical scavenging activity method to determine antioxidant activity. Acid pre-treatment stage and ultrafiltration caused an effective removal of phenolic compounds yielding on the one hand APC with lower phenolic content than the ones obtained by CP. On the other hand, the acid extract and the whey obtained presented high phenolic content and antioxidant activity and could be used as additives to increased this parameters in food. Finally, evaluated processes could be used to obtain several products (concentrates, whey, extracts) with different phenolic content and antioxidant activity suitable for different applications in food industry. (C) 2013 Elsevier Ltd. All rights reserved.1226267Projects: CAI + D type IIUNL [PI 57-283]ANCYPT - UNL [PICTO 35831, PICTO 36237]UNL [PI 57-283]ANCYPT - UNL [PICTO 35831, PICTO 36237

Comparison between isoelectric precipitation and ultrafiltration processes to obtain Amaranth mantegazzianus protein concentrates at pilot plant scale

The aim of this study was to compare protein yield, protein concentration and physicochemical characteristics of Amaranth mantegazzianus protein concentrates (APC) obtained at pilot-scale by a conventional process (CP) (alkaline extraction and isoelectric precipitation) and two alternative processes (AP): (1) acid pre-treatment process combined with isoelectric precipitation and (2) acid pre-treatment process combined with ultrafiltration. Although AP resulted in higher protein concentration, protein yield was lower than in CP. SDS-PAGE and size-exclusion chromatography showed high molecular weight fractions only for isoelectric precipitation concentrates (obtained by CP and AP). The amino acids concentration, especially phenylalanine, isoleucine and methionine, increased in all protein concentrates respect to the amaranth flour. Particularly, the product obtained by ultrafiltration was rich in phenylalanine and lysine, and presented no limiting amino acid with respect to the recommendation of the Food and Agriculture Organization of the United Nations (FAO). In conclusion, process (2) improved protein concentration and nutritional quality (balanced amino acid composition) of A. mantegazzianus protein concentrates respect to CP and process (1), suggesting that the ultrafiltration process is a viable alternative to conventional process and a promising method for obtaining protein concentrates. (C) 2012 Elsevier Ltd. All rights reserved.112428829

Total phenolic content and antioxidant activity of different streams resulting from pilot-plant processes to obtain amaranthus mantegazzianus protein concentrates

Antioxidant properties of different pilot-plant process streams to obtain Amaranthus mantegazzianus protein concentrates (APC) were evaluated. Conventional process (CP) (alkaline extraction and isoelectric precipitation) and two alternative processes (APs): (1) acid pre-treatment stage combined with isoelectric precipitation and (2) acid pre-treatment stage combined with ultrafiltration were applied at pilot-plant scale to obtain APC. Methanol and water extracts of APC and other fractions obtained in the processes were evaluated by Folin–Ciocalteau method in order to determine total phenolic content and by DPPH radical scavenging activity method to determine antioxidant activity. Acid pre-treatment stage and ultrafiltration caused an effective removal of phenolic compounds yielding on the one hand APC with lower phenolic content than the ones obtained by CP. On the other hand, the acid extract and the whey obtained presented high phenolic content and antioxidant activity and could be used as additives to increased this parameters in food. Finally, evaluated processes could be used to obtain several products (concentrates, whey, extracts) with different phenolic content and antioxidant activity suitable for different applications in food industry122626

Total phenolic content and antioxidant activity of different streams resulting from pilot-plant processes to obtain Amaranthus mantegazzianus protein concentrates

Antioxidant properties of different pilot-plant process streams to obtain Amaranthus mantegazzianus protein concentrates (APC) were evaluated. Conventional process (CP) (alkaline extraction and isoelectric precipitation) and two alternative processes (APs): (1) acid pre-treatment stage combined with isoelectric precipitation and (2) acid pre-treatment stage combined with ultrafiltration were applied at pilot-plant scale to obtain APC. Methanol and water extracts of APC and other fractions obtained in the processes were evaluated by Folin–Ciocalteau method in order to determine total phenolic content and by DPPH radical scavenging activity method to determine antioxidant activity. Acid pre-treatment stage and ultrafiltration caused an effective removal of phenolic compounds yielding on the one hand APC with lower phenolic content than the ones obtained by CP. On the other hand, the acid extract and the whey obtained presented high phenolic content and antioxidant activity and could be used as additives to increased this parameters in food. Finally, evaluated processes could be used to obtain several products (concentrates, whey, extracts) with different phenolic content and antioxidant activity suitable for different applications in food industry.Fil: Castel, María Virginia. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Andrich, Oscar. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaFil: Netto, Flávia M.. Universidade Estadual de Campinas. Facultad de Engenharia de Alimentos. Departamento de Tecnología de Alimentos; BrasilFil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Carrara, Carlos Roberto. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentin

Proyecto de Extensión Promoción de la Agroecología: Un diagnóstico adaptado para un contexto diferente

El proyecto de extensión: “Promoción de la agroecología, otro modelo de producción es posible” es de carácter regional y se desarrolla en el centro de la provincia de Buenos Aires, Argentina. El objetivo de este trabajo fue diagnosticar la situación actual de la agroecología en la región, es decir, intereses, necesidades, dificultades y beneficios tanto en la producción como en el consumo de alimentos. El proyecto se realiza desde una metodología de investigación acción participativa y la modalidad elegida para caracterizar la situación actual de la agroecología en la región de influencia de la UNCPBA (Universidad Nacional del Centro de la Provincia de Buenos Aires) fue la de diagnóstico participativo. Las circunstancias vinculadas a la pandemia modificaron la modalidad del diagnóstico inicialmente planteada mediante un taller presencial con los diferentes actores de interés, a la modalidad virtual a través de una encuesta online de manera colaborativa entre las Instituciones participantes y el grupo de productores. Los principales resultados arrojan una alta receptividad, respondieron 492 personas entre productores, técnicos, docentes, estudiantes y otras ocupaciones. Esto demuestra un gran interés en la producción y el consumo de productos agroecológicos.The extension project, “Promotion of agroecology, different production model is possible” has a regional in nature and takes place in the centre of the province of Buenos Aires, Argentina. The objective of this work was to diagnose the current situation of agroecology in the region, such as interests, needs, difficulties and benefits in both food production and food consumption. The project is carried out from a participatory action research methodology and the modality which was initially proposed to characterize the current situation of agroecology in the region of influence of the UNCPBA (National University of the Centre of the Province of Buenos Aires) was that of participatory diagnosis. The circumstances related to the pandemic changed the diagnostic modality initially proposed through a face-to-face workshop with the different stakeholders, through an online collaborative survey between the participating institutions and the group of producers. The main results show a high receptivity, 492 people responded, including producers, technicians, teachers, students and other occupations. This shows a great interest in the production and consumption of agro-ecological products.Fil: Block, Karina Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Iturralde Elortegui, María del Rosario Margarita. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Zamora, Martín Sergio. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Martinefsky, María Julia. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Wynne, María Josefina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Oyesqui, Lía Ana Eloísa. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Andrich , Santiago. No especifíca;Fil: Giaquinta, Alejandro Enzo. No especifíca;Fil: Vazquez, Patricia Susana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Humanas. Centro de Estudios Sociales de América Latina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; ArgentinaFil: Farrando, Luis Adrián. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaVIII Congreso Latinoamericano de AgroecologíaMontevideoUruguayUniversidad de la República. Facultad de Agronomía. Departamento de Sistemas AmbientalesSociedad Científica Latinoamericana de Agroecologí

Characterization by high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry of the lipid fraction of Spirulina platensis pressurized ethanol extract

Microalgae have been suggested as a potential source for new functional ingredients, making possible the development of new functional foods from natural origin. Among the natural ingredients, polyunsaturated fatty acids (PUFAs) have generally been identified as an interesting group of compounds with biological activity, mainly related to their anti-inflammatory properties. In this regard, the use of environmentally friendly extraction procedures (e.g. pressurized liquid extraction, PLE) to obtain such natural ingredients is also becoming necessary. In this work, an exhaustive characterization of the lipid fraction of a pressurized ethanolic extract of the microalga Spirulina platensis is carried out. To achieve this objective high-performance liquid chromatography (HPLC) coupled to quadrupole time-of-flight mass spectrometry (QTOF-MS) is employed. The use of the QTOF analyzer allows the selection and isolation of precursor ions as well as providing the high efficiency, sensitivity and mass accuracy required. By means of this powerful hyphenated technique, it was possible to identify several polar lipids in an extract of S. platensis (some of them, to our knowledge, described for the first time in this work), including four free fatty acids, four monogalactosyl monoacylglycerols, three phosphatidylglycerols and two sulfoquinovosyl diacylglycerols.M.H. thanks MCYT for a FPI grant. This work was financed by Spanish Ministry of Education (AGL2005-06726-C04-01/ 02) and Comunidad Auto´noma de Madrid (S-0505/AGR/ 000153) projects.Peer reviewe