181 research outputs found
Primary sand-dune plant community and soil properties during the west-coast India monsoon
A seven-station interrupted belt transect was established that followed a previously observed plant zonation pattern across an aggrading primary coastal dune system in the dry tropical region of west-coast India. The dominant weather pattern is monsoon from June to November, followed by hot and dry winter months when rainfall is scarce. Physical and chemical soil characteristics in each of the stations were analysed on five separate occasions, the first before the onset of monsoon, three during and the last post-monsoon. The plant community pattern was confirmed by quadrat survey. A pH gradient decreased with distance from the shoreline. Nutrient concentrations were deficient, increasing only in small amounts until the furthest station inland. At that location, there was a distinct and abrupt pedological transition zone from psammite to humic soils. There was a significant increase over previous stations in mean organic matter, ammonium nitrate and soil-water retention, although the increase in real terms was small. ANOVA showed significant variation in electrical conductivity, phosphorus, calcium, magnesium and sodium concentrations over time. There was no relationship between soil chemistry characteristics and plant community structure over the transect. Ipomoea pes-caprae and Spinifex littoreus were restricted to the foredunes, the leguminous forb Alysicarpus vaginalis and Perotis indica to the two stations furthest from the strand. Ischaemum indicum, a C4 perennial grass species adopting an ephemeral strategy was, in contrast, ubiquitous to all stations
Characterization of Roman glass tesserae from the Coriglia excavation site (Italy) via energy-dispersive X-ray fluorescence spectrometry and Raman spectroscopy
The combined use of handheld energy-dispersive X-ray fluorescence spectrometry, Raman spectroscopy, and micro-energy-dispersive X-ray fluorescence spectrometry permitted the characterization of Roman glass tesserae excavation from the Coriglia (Italy) archeological site. Analyses of ten different glass colors were conducted as spot analyses on intact samples and as both spot analyses and line scans on select cross-sectioned samples. The elemental and molecular information gained from these spectral measurements allowed for the qualitative chemical characterization of the bulk glass, decolorants, opacifiers, and coloring agents. The use of an antimony opacifier in many of the samples supports the late Imperial phasing as determined through numismatic, fresco, ceramics, and architectural evidence. And dealinization of the exterior glass layers caused by the burial environment was confirmed
Real structured singular value synthesis using the scaled Popov criterion
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/77238/1/AIAA-21537-608.pd
Generalized Cross-Validation as a Method of Hyperparameter Search for MTGV Regularization
The concept of generalized cross-validation (GCV) is applied to modified
total generalized variation (MTGV) regularization. Current implementations of
the MTGV regularization rely on manual (or semi-manual) hyperparameter
optimization, which is both time-consuming and subject to bias. The combination
of MTGV-regularization and GCV allows for a straightforward hyperparameter
search during regularization. This significantly increases the efficiency of
the MTGV-method, because it limits the number of hyperparameters, which have to
be tested and, improves the practicality of MTGV regularization as a standard
technique for inversion of NMR signals. The combined method is applied to
simulated and experimental NMR data and the resulting reconstructed
distributions are presented. It is shown that for all data sets studied the
proposed combination of MTGV and GCV minimizes the GCV score allowing an
optimal hyperparameter choice
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De novo formation of an aggregation pheromone precursor by an isoprenyl diphosphate synthase-related terpene synthase in the harlequin bug.
Insects use a diverse array of specialized terpene metabolites as pheromones in intraspecific interactions. In contrast to plants and microbes, which employ enzymes called terpene synthases (TPSs) to synthesize terpene metabolites, limited information from few species is available about the enzymatic mechanisms underlying terpene pheromone biosynthesis in insects. Several stink bugs (Hemiptera: Pentatomidae), among them severe agricultural pests, release 15-carbon sesquiterpenes with a bisabolene skeleton as sex or aggregation pheromones. The harlequin bug, Murgantia histrionica, a specialist pest of crucifers, uses two stereoisomers of 10,11-epoxy-1-bisabolen-3-ol as a male-released aggregation pheromone called murgantiol. We show that MhTPS (MhIDS-1), an enzyme unrelated to plant and microbial TPSs but with similarity to trans-isoprenyl diphosphate synthases (IDS) of the core terpene biosynthetic pathway, catalyzes the formation of (1S,6S,7R)-1,10-bisaboladien-1-ol (sesquipiperitol) as a terpene intermediate in murgantiol biosynthesis. Sesquipiperitol, a so-far-unknown compound in animals, also occurs in plants, indicating convergent evolution in the biosynthesis of this sesquiterpene. RNAi-mediated knockdown of MhTPS mRNA confirmed the role of MhTPS in murgantiol biosynthesis. MhTPS expression is highly specific to tissues lining the cuticle of the abdominal sternites of mature males. Phylogenetic analysis suggests that MhTPS is derived from a trans-IDS progenitor and diverged from bona fide trans-IDS proteins including MhIDS-2, which functions as an (E,E)-farnesyl diphosphate (FPP) synthase. Structure-guided mutagenesis revealed several residues critical to MhTPS and MhFPPS activity. The emergence of an IDS-like protein with TPS activity in M. histrionica demonstrates that de novo terpene biosynthesis evolved in the Hemiptera in an adaptation for intraspecific communication
Identification and characterization of Src SH3 ligands from phage-displayed random peptide libraries.
We have used the Src homology 3 (SH3) domain to screen two phage-displayed random peptide libraries, each containing 2 x 10(8) unique members, and have identified a series of high affinity peptide ligands. The peptides possess similar proline-rich regions, which yield a consensus Src SH3-binding motif of RPLPPLP. We have confirmed this motif by screening a phage-displayed peptide library biased for SH3 ligands and identifying the same consensus sequence. Binding studies using synthetic peptides suggest that the RPLPPLP motif is important for SH3 binding and confers specificity for the Src SH3 domain, and that residues which flank the motif may also contribute to binding. Peptides that contain the RPLPPLP motif compete Src, but not Abl or phospholipase C gamma, SH3 interactions with SH3-binding proteins from cell lysates (IC50 = 1-5 microM). Furthermore, RPLPPLP-related peptides are able to accelerate progesterone-induced maturation of Xenopus laevis oocytes. A similar acceleration has been observed in oocytes treated with activated, but not normal, Xenopus Src, suggesting the possibility that the peptides are able to antagonize the negative regulation of Src activity by Src SH3 in vivo
Identification of Novel Human WW Domain-containing Proteins by Cloning of Ligand Targets
A recently described protein module consisting of 35-40 semiconserved residues, termed the WW domain, has been identified in a number of diverse proteins including dystrophin and Yes-associated protein (YAP). Two putative ligands of YAP, termed WBP-1 and WBP-2, have been found previously to contain several short peptide regions consisting of PPPPY residues (PY motif) that mediate binding to the WW domain of YAP. Although the function(s) of the WW domain remain to be elucidated, these observations strongly support a role for the WW domain in protein-protein interactions. Here we report the isolation of three novel human cDNAs encoding a total of nine WW domains, using a newly developed approach termed COLT (cloning of ligand targets), in which the rapid cloning of modular protein domains is accomplished by screening cDNA expression libraries with specific peptide ligands. Two of the new genes identified appear to be members of a family of proteins, including Rsp5 and Nedd-4, which have ubiquitin-protein ligase activity. In addition, we demonstrate that peptides corresponding to PY and PY-like motifs present in several known signaling or regulatory proteins, including RasGAP, AP-2, p53BP-2 (p53-binding protein-2), interleukin-6 receptor-alpha, chloride channel CLCN5, and epithelial sodium channel ENaC, can selectively bind to certain of these novel WW domains
The Fading Optical Counterpart of GRB~970228, Six Months and One Year Later
We report on observations of the fading optical counterpart of the gamma-ray
burst GRB 970228, made with the Hubble Space Telescope STIS CCD approximately
six months after outburst and with the HST/NICMOS and Keck/NIRC approximately
one year after outburst. The unresolved counterpart is detected by STIS at
V=28.0 +/- 0.25, consistent with a continued power-law decline with exponent
-1.14 +/- 0.05. The counterpart is located within, but near the edge of, a
faint extended source with diameter ~0."8 and integrated magnitude V=25.8 +/-
0.25. A reanalysis of HST and NTT observations performed shortly after the
burst shows no evidence of proper motion of the point source or fading of the
extended emission. Only the extended source is visible in the NICMOS images
with a magnitude of H=23.3 +/- 0.1. The Keck observations find K = 22.8 +/-
0.3. Several distinct and independent means of deriving the foreground
extinction in the direction of GRB 970228 all agree with A_V = 0.75 +/- 0.2.
After adjusting for Galactic extinction, we find that the size of the observed
extended emission is consistent with that of galaxies of comparable magnitude
found in the Hubble Deep Field (HDF) and other deep HST images. Only 2% of the
sky is covered by galaxies of similar or greater surface brightness; therefore
the extended source is almost certainly the host galaxy. Additionally, we find
that the extinction-corrected V - H and V - K colors of the host are as blue as
any galaxy of comparable or brighter magnitude in the HDF. Taken in concert
with recent observations of GRB 970508, GRB 971214, and GRB 980703 our work
suggests that all four GRBs with spectroscopic identification or deep
multicolor broad-band imaging of the host lie in rapidly star-forming galaxies.Comment: 24 pages, Latex, 4 PostScript figures, to appear in the May 10 issue
of The Astrophysical Journal (Note: displayed abstract is abridged
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