A Checklist of the Stoneflies, or Plecoptera, of Indiana

Examination of specimens and published records has indicated the presence of 61 species of Plecoptera in Indiana. Annotations include the recognition of another 14 species which should eventually be taken within the state, references to species keys and descriptions, and comments concerning doubtfully recorded species

Inversion of Parahermitian matrices

Parahermitian matrices arise in broadband multiple-input multiple-output (MIMO) systems or array processing, and require inversion in some instances. In this paper, we apply a polynomial eigenvalue decomposition obtained by the sequential best rotation algorithm to decompose a parahermitian matrix into a product of two paraunitary, i.e.lossless and easily invertible matrices, and a diagonal polynomial matrix. The inversion of the overall parahermitian matrix therefore reduces to the inversion of auto-correlation sequences in this diagonal matrix. We investigate a number of different approaches to obtain this inversion, and and assessment of the numerical stability and complexity of the inversion process

Long range experimental hydrologic forecasting for the eastern U.S.

We explore a strategy for long-range hydrologic forecasting that uses ensemble climate model forecasts as input to a macroscale hydrologic model to produce runoff and streamflow forecasts at spatial and temporal scales appropriate for water management. Monthly ensemble climate model forecasts produced by the National Centers for Environmental Prediction/Climate Prediction Center global spectral model (GSM) are bias corrected, downscaled to 1/8° horizontal resolution, and disaggregated to a daily time step for input to the Variable Infiltration Capacity hydrologic model. Bias correction is effected by evaluating the GSM ensemble forecast variables as percentiles relative to the GSM model climatology and then extracting the percentiles\u27 associated variable values instead from the observed climatology. The monthly meteorological forecasts are then interpolated to the finer hydrologic model scale, at which a daily signal that preserves the forecast anomaly is imposed through resampling of the historic record. With the resulting monthly runoff and streamflow forecasts for the East Coast and Ohio River basin, we evaluate the bias correction and resampling approaches during the southeastern United States drought from May to August 2000 and also for the El Niño conditions of December 1997 to February 1998. For the summer 2000 study period, persistence in anomalous initial hydrologic states predominates in determining the hydrologic forecasts. In contrast, the El Niño-condition hydrologic forecasts derive direction both from the climate model forecast signal and the antecedent land surface state. From a qualitative standpoint the hydrologic forecasting strategy appears successful in translating climate forecast signals to hydrologic variables of interest for water management

Functional profiles of orphan membrane transporters in the life cycle of the malaria parasite

Assigning function to orphan membrane transport proteins and prioritizing candidates for detailed biochemical characterization remain fundamental challenges and are particularly important for medically relevant pathogens, such as malaria parasites. Here we present a comprehensive genetic analysis of 35 orphan transport proteins of Plasmodium berghei during its life cycle in mice and Anopheles mosquitoes. Six genes, including four candidate aminophospholipid transporters, are refractory to gene deletion, indicative of essential functions. We generate and phenotypically characterize 29 mutant strains with deletions of individual transporter genes. Whereas seven genes appear to be dispensable under the experimental conditions tested, deletion of any of the 22 other genes leads to specific defects in life cycle progression in vivo and/or host transition. Our study provides growing support for a potential link between heavy metal homeostasis and host switching and reveals potential targets for rational design of new intervention strategies against malaria

ARGES: an Expert System for Fault Diagnosis Within Space-Based ECLS Systems

ARGES (Atmospheric Revitalization Group Expert System) is a demonstration prototype expert system for fault management for the Solid Amine, Water Desorbed (SAWD) CO2 removal assembly, associated with the Environmental Control and Life Support (ECLS) System. ARGES monitors and reduces data in real time from either the SAWD controller or a simulation of the SAWD assembly. It can detect gradual degradations or predict failures. This allows graceful shutdown and scheduled maintenance, which reduces crew maintenance overhead. Status and fault information is presented in a user interface that simulates what would be seen by a crewperson. The user interface employs animated color graphics and an object oriented approach to provide detailed status information, fault identification, and explanation of reasoning in a rapidly assimulated manner. In addition, ARGES recommends possible courses of action for predicted and actual faults. ARGES is seen as a forerunner of AI-based fault management systems for manned space systems

Copper-transporting ATPase is important for malaria parasite fertility

Homeostasis of the trace element copper is essential to all eukaryotic life. Copper serves as a cofactor in metalloenzymes and catalyses electron transfer reactions as well as the generation of potentially toxic reactive oxygen species. Here, we describe the functional characterization of an evolutionarily highly conserved, predicted copper-transporting P-type ATPase (CuTP) in the murine malaria model parasite Plasmodium berghei. Live imaging of a parasite line expressing a fluorescently tagged CuTP demonstrated that CuTP is predominantly located in vesicular bodies of the parasite. A P. berghei loss-of-function mutant line was readily obtained and showed no apparent defect in in vivo blood stage growth. Parasite transmission through the mosquito vector was severely affected, but not entirely abolished. We show that male and female gametocytes are abundant in cutp− parasites, but activation of male microgametes and exflagellation were strongly impaired. This specific defect could be mimicked by addition of the copper chelator neocuproine to wild-type gametocytes. A cross-fertilization assay demonstrated that female fertility was also severely abrogated. In conclusion, we provide experimental genetic and pharmacological evidence that a healthy copper homeostasis is critical to malaria parasite fertility of both genders of gametocyte and, hence, to transmission to the mosquito vector