18 research outputs found
Elucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin
The interaction of peroxidized cardiolipin with
ferrocytochrome c induces two kinetically and chemically
distinct processes. The first is a rapid oxidation of ferrocytochrome
c, followed by a slower, irreversible disruption
of heme c. The oxidation of ferrocytochrome c by peroxidized
cardiolipin is explained by a Fenton-type reaction.
Heme scission is a consequence of the radical-mediated
reactions initiated by the interaction of ferric heme iron
with peroxidized cardiolipin. Simultaneously with the
heme c disruption, generation of hydroxyl radical is
detected by EPR spectroscopy using the spin trapping
technique. The resulting apocytochrome c sediments as a
heterogeneous mixture of high aggregates, as judged by
sedimentation analysis. Both the oxidative process and the
destructive process were suppressed by nonionic detergents
and/or high ionic strength. The mechanism for generating
radicals and heme rupture is presented