Räumliche Variabilität und zeitliche Trends des Trockenstressrisikos von Kiefernstandorten in Mecklenburg-Vorpommern

Die Bodenwasserverfügbarkeit bestimmt zu einem hohen Maße die Vitalität von Bäumen und Waldstandorten. In einigen Teilen Euro-pas wurde in den letzten Jahrzehnten eine häufigere Anzahl von Trockenperioden beobachtet, die bereits das Baumwachstum negativ beeinflusst haben. Es wurden langfristige Trends von Bodentrockenheit unter Kiefernstandorten entlang des klimatischen Gradienten in Mecklenburg-Vorpommern mit dem physikalisch basierten Modell LWF-BROOK90 untersucht. Die Ergebnisse zeigen eine zunehmende Anzahl von Trockenstresstagen ostwärts mit abnehmenden Niederschlagssummen. Die klimatischen Unterschiede innerhalb des Landes werden teilweise von den spezifischen Standortbedingungen und Bodeneigenschaften überprägt. Bodentrockenheit hat in den letzten Jahrzehnten deutlich zugenommen, wobei die östlichen Standorte stärker als die westlichen betroffen sind. Die zunehmende Bodentrockenheit konnte auf einen erhöhten Verdunstungsanspruch der Atmosphäre zurückgeführt werden aufgrund höherer Temperaturen in Kombination mit leicht abnehmenden Niederschlägen während der Sommermonate. Um die negativen Auswirkungen künftiger klimatischer Änderungen zu minimieren, sollten Anpassungsmaßnahmen vorzugsweise im Osten des Landes durchgeführt werden

Photosynthesis in C3-C4 intermediate Moricandia species.

Evolution of C4 photosynthesis is not distributed evenly in the plant kingdom. Particularly interesting is the situation in the Brassicaceae, because the family contains no C4 species, but several C3-C4 intermediates, mainly in the genus Moricandia Investigation of leaf anatomy, gas exchange parameters, the metabolome, and the transcriptome of two C3-C4 intermediate Moricandia species, M. arvensis and M. suffruticosa, and their close C3 relative M. moricandioides enabled us to unravel the specific C3-C4 characteristics in these Moricandia lines. Reduced CO2 compensation points in these lines were accompanied by anatomical adjustments, such as centripetal concentration of organelles in the bundle sheath, and metabolic adjustments, such as the balancing of C and N metabolism between mesophyll and bundle sheath cells by multiple pathways. Evolution from C3 to C3-C4 intermediacy was probably facilitated first by loss of one copy of the glycine decarboxylase P-protein, followed by dominant activity of a bundle sheath-specific element in its promoter. In contrast to recent models, installation of the C3-C4 pathway was not accompanied by enhanced activity of the C4 cycle. Our results indicate that metabolic limitations connected to N metabolism or anatomical limitations connected to vein density could have constrained evolution of C4 in Moricandia

Manipulating photorespiration to increase plant productivity:recent advances and perspectives for crop improvement

Recycling of the 2-phosphoglycolate generated by the oxygenase reaction of Rubisco requires a complex and energy-consuming set of reactions collectively known as the photorespiratory cycle. Several approaches aimed at reducing the rates of photorespiratory energy or carbon loss have been proposed, based either on screening for natural variation or by means of genetic engineering. Recent work indicates that plant yield can be substantially improved by the alteration of photorespiratory fluxes or by engineering artificial bypasses to photorespiration. However, there is also evidence indicating that, under certain environmental and/or nutritional conditions, reduced photorespiratory capacity may be detrimental to plant performance. Here we summarize recent advances obtained in photorespiratory engineering and discuss prospects for these advances to be transferred to major crops to help address the globally increasing demand for food and biomass production

Photorespiration Is Crucial for Dynamic Response of Photosynthetic Metabolism and Stomatal Movement to Altered CO2 Availability

Eisenhut M, Bräutigam A, Timm S, et al. Photorespiration Is Crucial for Dynamic Response of Photosynthetic Metabolism and Stomatal Movement to Altered CO2 Availability. Molecular Plant. 2017;10(1):47-61.The photorespiratory pathway or photorespiration is an essential process in oxygenic photosynthetic organisms, which can reduce the efficiency of photosynthetic carbon assimilation and is hence frequently considered as a wasteful process. By comparing the response of the wild-type plants and mutants impaired in photorespiration to a shift in ambient CO2 concentrations, we demonstrate that photorespiration also plays a beneficial role during short-term acclimation to reduced CO2 availability. The wild-type plants responded with few differentially expressed genes, mostly involved in drought stress, which is likely a consequence of enhanced opening of stomata and concomitant water loss upon a shift toward low CO2. In contrast, mutants with impaired activity of photorespiratory enzymes were highly stressed and not able to adjust stomatal conductance to reduced external CO2 availability. The transcriptional response of mutant plants was congruent, indicating a general reprogramming to deal with the consequences of reduced CO2 availability, signaled by enhanced oxygenation of ribulose-1,5-bisphosphate and amplified by the artificially impaired photorespiratory metabolism. Central in this reprogramming was the pronounced reallocation of resources from growth processes to stress responses. Taken together, our results indicate that unrestricted photorespiratory metabolism is a prerequisite for rapid physiological acclimation to a reduction in CO2 availability

Impact of Filters to Reduce Phosphorus Losses: Field Observations and Modelling Tests in Tile-Drained Lowland Catchments

In this study, we analyzed Dissolved Reactive Phosphorus (DRP) and Total Phosphorus (TP) concentration dynamics over two years in surface waters of five nested catchments in northeastern Germany. Based on this, we constructed a filter box filled with iron-coated sand for Phosphorus (P) removal at the edge of a tile-drained field. Results of the filter box experiment were used for a model scenario analysis aiming at evaluating the P removal potential at catchment scale. DRP and TP concentrations were generally low but they exceeded occasionally target values. Results of the filter box experiment indicated that 28% of the TP load could be retained but the DRP load reduction was negligible. We assume that DRP could not be reduced due to short residence times and high flow dynamics. Instead, particulate P fractions were probably retained mechanically by the filter material. The scenario analysis revealed that the P removal potential of such filters are highest in areas, in which tile drainage water is the dominant P source. At a larger spatial scale, in which other P (point) sources are likewise important, edge-of-field P filters can only be one part of an integrated catchment strategy involving a variety of measures to reduce P losses

Hydro-Meteorological Trends in the Upper Omo-Ghibe River Basin, Ethiopia

This study aimed to assess trends of hydro-meteorological variables in the Upper Omo-Ghibe river basin, Ethiopia. Data records from eleven rainfall, eight air temperature, and five streamflow stations between 1981 to 2008 were investigated. The trends and change points were evaluated for different periods of time depending on data availability. Mann-Kendall and Pettit tests were used to identify trends and change points at a 5% significance level. The tests were applied to mean annual, monthly and seasonal time scales. Rainfall exhibited statistically decreasing trends at a mean annual time scale, while seasonal rainfall depicted heterogeneous results in both directions. For the majority of the stations, air temperature showed statistically significant increasing trends. The magnitude of change in temperature for mean annual, wet and dry season has increased about 0.48, 0.46, and 0.61 °C per decade for Jimma station. The Pettit test revealed that the late 1980s and 1990s were the change points. There is generally a decreasing trend in streamflow. The decline in annual rainfall and rise in temperature affected the streamflow negatively. Overall, the results indicate that trend sand change point times varied considerably across the stations and catchments. The identified significant trends can help to support planning decisions for water management

Evolution of Photorespiratory Glycolate Oxidase among Archaeplastida

Photorespiration has been shown to be essential for all oxygenic phototrophs in the present-day oxygen-containing atmosphere. The strong similarity of the photorespiratory cycle in cyanobacteria and plants led to the hypothesis that oxygenic photosynthesis and photorespiration co-evolved in cyanobacteria, and then entered the eukaryotic algal lineages up to land plants via endosymbiosis. However, the evolutionary origin of the photorespiratory enzyme glycolate oxidase (GOX) is controversial, which challenges the common origin hypothesis. Here, we tested this hypothesis using phylogenetic and biochemical approaches with broad taxon sampling. Phylogenetic analysis supported the view that a cyanobacterial GOX-like protein of the 2-hydroxy-acid oxidase family most likely served as an ancestor for GOX in all eukaryotes. Furthermore, our results strongly indicate that GOX was recruited to the photorespiratory metabolism at the origin of Archaeplastida, because we verified that Glaucophyta, Rhodophyta, and Streptophyta all express GOX enzymes with preference for the substrate glycolate. Moreover, an ancestral protein synthetically derived from the node separating all prokaryotic from eukaryotic GOX-like proteins also preferred glycolate over l-lactate. These results support the notion that a cyanobacterial ancestral protein laid the foundation for the evolution of photorespiratory GOX enzymes in modern eukaryotic phototrophs

Evolution of photorespiration from cyanobacteria to land plants, considering protein phylogenies and acquisition of carbon concentrating mechanisms

Photorespiration is intimately linked to oxygenic photosynthesis. Here, the evolution of photorespiration in different lineages of oxygenic phototrophs and its relation to the appearance of carbon concentrating mechanisms is discussed.Photorespiration and oxygenic photosynthesis are intimately linked processes. It has been shown that under the present day atmospheric conditions cyanobacteria and all eukaryotic phototrophs need functional photorespiration to grow autotrophically. The question arises as to when this essential partnership evolved, i.e. can we assume a coevolution of both processes from the beginning or did photorespiration evolve later to compensate for the generation of 2-phosphoglycolate (2PG) due to Rubisco's oxygenase reaction? This question is mainly discussed here using phylogenetic analysis of proteins involved in the 2PG metabolism and the acquisition of different carbon concentrating mechanisms (CCMs). The phylogenies revealed that the enzymes involved in the photorespiration of vascular plants have diverse origins, with some proteins acquired from cyanobacteria as ancestors of the chloroplasts and others from heterotrophic bacteria as ancestors of mitochondria in the plant cell. Only phosphoglycolate phosphatase was found to originate from Archaea. Notably glaucophyte algae, the earliest branching lineage of Archaeplastida, contain more photorespiratory enzymes of cyanobacterial origin than other algal lineages or land plants indicating a larger initial contribution of cyanobacterial-derived proteins to eukaryotic photorespiration. The acquisition of CCMs is discussed as a proxy for assessing the timing of periods when photorespiratory activity may have been enhanced. The existence of CCMs also had marked influence on the structure and function of photorespiration. Here, we discuss evidence for an early and continuous coevolution of photorespiration, CCMs and photosynthesis starting from cyanobacteria via algae, to land plants